Restriction map for
pFN21AA1226
(Circular) (Six-base) MAPSORT of: pFN21AA1226.seq Check: 1415 from: 1 to: 6817
pFN21AA1226 6817 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3621 4627 278
Size: 53 83 186 936 2085 1006 2468
Fragments arranged by size:
2468 2085 1006 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4160 1937
Size: 2223 4594
Acc65I G'GTAC_C
Cuts at: 4138 4138
Size: 6817
AclI AA'CG_TT
Cuts at: 1563 4944 5317 1563
Size: 3381 373 3063
Fragments arranged by size:
3381 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 3195 4880 6264 6532 1632
Size: 458 1105 1685 1384 268 1917
Fragments arranged by size:
1917 1685 1384 1105 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6620
AflIII A'CryG_T
Cuts at: 1203 5716 6693 1203
Size: 4513 977 1327
Fragments arranged by size:
4513 1327 977
AgeI A'CCGG_T
Cuts at: 5648 5648
Size: 6817
AhdI GACnn_n'nnGTC
Cuts at: 5546 5546
Size: 6817
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6817
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3872 4061 1184
Size: 2688 189 3940
Fragments arranged by size:
3940 2688 189
AlwNI CAG_nnn'CTG
Cuts at: 2278 3413 4560 6132 2278
Size: 1135 1147 1572 2963
Fragments arranged by size:
2963 1572 1147 1135
ApaLI G'TGCA_C
Cuts at: 3525 4874 6030 3525
Size: 1349 1156 4312
Fragments arranged by size:
4312 1349 1156
ApoI r'AATT_y
Cuts at: 1493 3019 4126 4352 4504 6613 1493
Size: 1526 1107 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 1107 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5371 160
Size: 872 4339 1606
Fragments arranged by size:
4339 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
AvaI C'yCGr_G
Cuts at: 1870 1943 4142 1870
Size: 73 2199 4545
Fragments arranged by size:
4545 2199 73
BamHI G'GATC_C
Cuts at: 4147 4514 4147
Size: 367 6450
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 3583 4138 5593 618
Size: 325 217 627 229 1567 555 1455 1842
Fragments arranged by size:
1842 1567 1455 627 555 325 229 217
BanII G_rGCy'C
Cuts at: 1391 2196 2538 4136 1391
Size: 805 342 1598 4072
Fragments arranged by size:
4072 1598 805 342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2109 3713 928
Size: 971 210 1604 4032
Fragments arranged by size:
4032 1604 971 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5008 5042 1903
Size: 34 3071 34 3678
Fragments arranged by size:
3678 3071 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2350 2783 3881 4709 5919 2350
Size: 433 1098 828 1210 3248
Fragments arranged by size:
3248 1210 1098 828 433
BclI T'GATC_A
Cuts at: 1548 3757 1548
Size: 2209 4608
BfrBI ATG'CAT
Cuts at: 3514 3514
Size: 6817
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5428 136
Size: 107 122 71 631 605 3756 1525
Fragments arranged by size:
3756 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6812 6812
Size: 6817
BlpI GC'TnA_GC
Cuts at: 4234 4234
Size: 6817
Bme1580I G_kGCm'C
Cuts at: 1790 3529 4878 6034 1790
Size: 1739 1349 1156 2573
Fragments arranged by size:
2573 1739 1349 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6817
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4548 5506 442
Size: 416 53 83 399 24 3131 958 1753
Fragments arranged by size:
3131 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3350 3828 5477 1239
Size: 2111 478 1649 2579
Fragments arranged by size:
2579 2111 1649 478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3101 3128 4942 5807 6105 3101
Size: 27 1814 865 298 3813
Fragments arranged by size:
3813 1814 865 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3368 5480 882
Size: 617 1869 2112 2219
Fragments arranged by size:
2219 2112 1869 617
BsaAI yAC'GTr
Cuts at: 493 1204 3508 4608 493
Size: 711 2304 1100 2702
Fragments arranged by size:
2702 2304 1100 711
BsaBI GATnn'nnATC
Cuts at: 6811 6811
Size: 6817
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3618 4624 5006 5641
Size: 53 83 186 936 2085 1006 382 635
Cuts at: 5641 275
Size: 1451
Fragments arranged by size:
2085 1451 1006 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4114 5250 5648 5922 6069 6480 4114
Size: 1136 398 274 147 411 4451
Fragments arranged by size:
4451 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6592
Fragments arranged by size:
6592 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2091 2204 2436 1186
Size: 905 113 232 5567
Fragments arranged by size:
5567 905 232 113
BseYI C'CCAG_C
Cuts at: 2331 6020 6516 2331
Size: 3689 496 2632
Fragments arranged by size:
3689 2632 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 1163
Size: 573 486 5758
Fragments arranged by size:
5758 573 486
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4267 5028 6056 1540
Size: 275 186 2266 761 1028 2301
Fragments arranged by size:
2301 2266 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2671 2939 3529 4878 4963 6034 2196
Size: 475 268 590 1349 85 1071 2979
Fragments arranged by size:
2979 1349 1071 590 475 268 85
BsmI GAATG_Cn'
Cuts at: 2238 3041 4328 4421 2238
Size: 803 1287 93 4634
Fragments arranged by size:
4634 1287 803 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2538 2671 2939 3529 4136 4878
Size: 399 406 342 133 268 590 607 742
Cuts at: 4878 4963 6034 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 607 590 406 399 342
268 133 85
BspEI T'CCGG_A
Cuts at: 6480 6480
Size: 6817
BspHI T'CATG_A
Cuts at: 2757 3493 4706 6436 2757
Size: 736 1213 1730 3138
Fragments arranged by size:
3138 1730 1213 736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3437 4172 844
Size: 2593 735 3489
Fragments arranged by size:
3489 2593 735
BsrBI CCG'CTC
Cuts at: 1394 1823 4704 1394
Size: 429 2881 3507
Fragments arranged by size:
3507 2881 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2118 5312 5486 65
Size: 1183 870 3194 174 1396
Fragments arranged by size:
3194 1396 1183 870 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5461 5648 1700
Size: 110 3651 187 2869
Fragments arranged by size:
3651 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6817
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6817
BssSI C'ACGA_G
Cuts at: 3419 4877 5889 3419
Size: 1458 1012 4347
Fragments arranged by size:
4347 1458 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3413 794
Size: 2619 4198
BstBI TT'CG_AA
Cuts at: 5621 5621
Size: 6817
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6817
BstXI CCAn_nnnn'nTGG
Cuts at: 3905 3905
Size: 6817
BstYI r'GATC_y
Cuts at: 1972 2155 3082 4025 4147 4514 4899 4916 6368
Size: 183 927 943 122 367 385 17 1452
Cuts at: 6368 6454 6466 6812 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 943 927 385 367 346 183
122 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2484 2484
Size: 6817
BtgI C'CryG_G
Cuts at: 513 1065 3933 513
Size: 552 2868 3397
Fragments arranged by size:
3397 2868 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3573 3765 487
Size: 683 56 2347 192 3539
Fragments arranged by size:
3539 2347 683 192 56
BtsI GCAGTG_nn'
Cuts at: 2858 2925 3414 4333 5127 5147 2858
Size: 67 489 919 794 20 4528
Fragments arranged by size:
4528 919 794 489 67 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3147 3182 529
Size: 35 2583 35 4164
Fragments arranged by size:
4164 2583 35 35
DraI TTT'AAA
Cuts at: 3958 4121 3958
Size: 163 6654
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5824 809
Size: 507 4508 1802
Fragments arranged by size:
4508 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2874 3895 4264
Size: 54 1425 50 275 18 1044 1021 369
Cuts at: 4264 5153 6782 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1044 1021 889 369 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4264 1537
Size: 275 2452 4090
Fragments arranged by size:
4090 2452 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2080 2109 2676 3771 4747 1353
Size: 727 29 567 1095 976 3423
Fragments arranged by size:
3423 1095 976 727 567 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5404 5778 5924 387
Size: 1064 138 3815 374 146 1280
Fragments arranged by size:
3815 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2194 2194
Size: 6817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 3195 3350 3828 4880 5477 6264
Size: 393 458 1105 155 478 1052 597 787
Cuts at: 6264 6532 1239
Size: 268 1524
Fragments arranged by size:
1524 1105 1052 787 597 478 458 393
268 155
EcoNI CCTnn'n_nnAGG
Cuts at: 2624 2624
Size: 6817
EcoRI G'AATT_C
Cuts at: 4126 4504 4126
Size: 378 6439
EcoRV GAT'ATC
Cuts at: 3911 3911
Size: 6817
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3169 3169
Size: 6817
FspI TGC'GCA
Cuts at: 5323 5323
Size: 6817
HaeII r_GCGC'y
Cuts at: 1678 5964 1678
Size: 4286 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 3220 3252 3572
Size: 32 135 32 514 32 1102 32 320
Cuts at: 3572 3604 3961 3993 5458 5490 5532 5564 1373
Size: 32 357 32 1465 32 42 32 2626
Fragments arranged by size:
2626 1465 1102 514 357 320 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3712 4161 4407 669
Size: 988 281 1774 449 246 3079
Fragments arranged by size:
3079 1774 988 449 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6817
HpaI GTT'AAC
Cuts at: 4407 4407
Size: 6817
KpnI G_GTAC'C
Cuts at: 4142 4142
Size: 6817
MfeI C'AATT_G
Cuts at: 3159 4416 3159
Size: 1257 5560
MluI A'CGCG_T
Cuts at: 6693 6693
Size: 6817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2209 3967 5931 6115 1305
Size: 904 1758 1964 184 2007
Fragments arranged by size:
2007 1964 1758 904 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2876 3897 10
Size: 54 1425 343 1044 1021 2930
Fragments arranged by size:
2930 1425 1044 1021 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3854 3938 4043 4775 5134 5293 518
Size: 648 2688 84 105 732 359 159 2042
Fragments arranged by size:
2688 2042 732 648 359 159 105 84
MspA1I CmG'CkG
Cuts at: 1606 3951 4233 4910 6058 6303 1606
Size: 2345 282 677 1148 245 2120
Fragments arranged by size:
2345 2120 1148 677 282 245
NcoI C'CATG_G
Cuts at: 513 1065 3933 513
Size: 552 2868 3397
Fragments arranged by size:
3397 2868 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6817
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6817
NotI GC'GGCC_GC
Cuts at: 4264 4264
Size: 6817
NsiI A_TGCA'T
Cuts at: 3516 3516
Size: 6817
NspI r_CATG'y
Cuts at: 1132 1229 2339 2903 4048 4112 4175 5720 1132
Size: 97 1110 564 1145 64 63 1545 2229
Fragments arranged by size:
2229 1545 1145 1110 564 97 64 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6817
PciI A'CATG_T
Cuts at: 5716 5716
Size: 6817
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3986 1108
Size: 2878 3939
PfoI T'CCnGG_A
Cuts at: 2057 2057
Size: 6817
PmeI GTTT'AAAC
Cuts at: 4121 4121
Size: 6817
PmlI CAC'GTG
Cuts at: 3508 3508
Size: 6817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4886 4918 6399 6431 4886
Size: 32 1481 32 5272
Fragments arranged by size:
5272 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6817
PsiI TTA'TAA
Cuts at: 3651 4387 3651
Size: 736 6081
PstI C_TGCA'G
Cuts at: 830 2274 4169 830
Size: 1444 1895 3478
Fragments arranged by size:
3478 1895 1444
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6817
PvuII CAG'CTG
Cuts at: 3951 3951
Size: 6817
SacI G_AGCT'C
Cuts at: 2196 2196
Size: 6817
SalI G'TCGA_C
Cuts at: 1936 4159 1936
Size: 2223 4594
SapI GCTCTTCn'nnn_
Cuts at: 2676 2676
Size: 6817
SbfI CC_TGCA'GG
Cuts at: 4169 4169
Size: 6817
ScaI AGT'ACT
Cuts at: 5065 6777 5065
Size: 1712 5105
SfcI C'TryA_G
Cuts at: 826 1045 2270 3285 4165 5300 5981 6172 826
Size: 219 1225 1015 880 1135 681 191 1471
Fragments arranged by size:
1471 1225 1135 1015 880 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6817
SmaI CCC'GGG
Cuts at: 1872 4144 1872
Size: 2272 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3116 3143 4921 5822 6084 820
Size: 197 926 1173 27 1778 901 262 1553
Fragments arranged by size:
1778 1553 1173 926 901 262 197 27
SnaBI TAC'GTA
Cuts at: 493 4608 493
Size: 4115 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6817
SphI G_CATG'C
Cuts at: 1132 4112 4175 1132
Size: 2980 63 3774
Fragments arranged by size:
3774 2980 63
SspI AAT'ATT
Cuts at: 5 52 3825 3902 4741 5
Size: 47 3773 77 839 2081
Fragments arranged by size:
3773 2081 839 77 47
StuI AGG'CCT
Cuts at: 1517 4104 1517
Size: 2587 4230
StyI C'CwwG_G
Cuts at: 513 1065 2652 2868 2988 3326 3933 4256 513
Size: 552 1587 216 120 338 607 323 3074
Fragments arranged by size:
3074 1587 607 552 338 323 216 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2981 3350 3598 4844 4861 5014 5199
Size: 1255 1401 369 248 1246 17 153 185
Cuts at: 5199 5644 325
Size: 445 1498
Fragments arranged by size:
1498 1401 1255 1246 445 369 248 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2369 2554 5063
Size: 274 80 33 51 1443 392 185 2509
Cuts at: 5063 6775 96
Size: 1712 138
Fragments arranged by size:
2509 1712 1443 392 274 185 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 2733 2765 3238 3270 3546 3578 6777
Size: 32 625 32 473 32 276 32 3199
Cuts at: 6777 6809 2076
Size: 32 2084
Fragments arranged by size:
3199 2084 625 473 276 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2909 1316
Size: 1593 5224
XbaI T'CTAG_A
Cuts at: 2959 4153 2959
Size: 1194 5623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2791 3781 2497
Size: 294 990 5533
Fragments arranged by size:
5533 990 294
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6817
XmaI C'CCGG_G
Cuts at: 1870 4142 1870
Size: 2272 4545
XmnI GAAnn'nnTTC
Cuts at: 4946 4946
Size: 6817
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3619 4625 276
Size: 53 83 186 936 2085 1006 2468
Fragments arranged by size:
2468 2085 1006 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI