(Circular) (Six-base) MAPSORT of: pFN21AA1181.seq Check: 1418 from: 1 to: 5575 pFN21AA1181 5575 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2217 3385 278 Size: 53 83 186 936 681 1168 2468 Fragments arranged by size: 2468 1168 936 681 186 83 53 AccI GT'mk_AC Cuts at: 1937 2918 1937 Size: 981 4594 Acc65I G'GTAC_C Cuts at: 2896 2896 Size: 5575 AclI AA'CG_TT Cuts at: 1563 3702 4075 1563 Size: 2139 373 3063 Fragments arranged by size: 3063 2139 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2002 2591 3638 5022 5290 1632 Size: 370 589 1047 1384 268 1917 Fragments arranged by size: 1917 1384 1047 589 370 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5378 AflIII A'CryG_T Cuts at: 1203 2397 4474 5451 1203 Size: 1194 2077 977 1327 Fragments arranged by size: 2077 1327 1194 977 AgeI A'CCGG_T Cuts at: 4406 4406 Size: 5575 AhdI GACnn_n'nnGTC Cuts at: 4304 4304 Size: 5575 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5575 AlwNI CAG_nnn'CTG Cuts at: 2749 2837 3318 4890 2749 Size: 88 481 1572 3434 Fragments arranged by size: 3434 1572 481 88 ApaLI G'TGCA_C Cuts at: 3632 4788 3632 Size: 1156 4419 ApoI r'AATT_y Cuts at: 1493 2884 3110 3262 5371 1493 Size: 1391 226 152 2109 1697 Fragments arranged by size: 2109 1697 1391 226 152 AseI AT'TA_AT Cuts at: 160 1032 4129 160 Size: 872 3097 1606 Fragments arranged by size: 3097 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 AvaI C'yCGr_G Cuts at: 1870 1943 2516 2900 1870 Size: 73 573 384 4545 Fragments arranged by size: 4545 573 384 73 BamHI G'GATC_C Cuts at: 2905 3272 2905 Size: 367 5208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 2896 4351 618 Size: 325 217 627 257 852 1455 1842 Fragments arranged by size: 1842 1455 852 627 325 257 217 BanII G_rGCy'C Cuts at: 1391 2136 2172 2894 1391 Size: 745 36 722 4072 Fragments arranged by size: 4072 745 722 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4604 BbvCI CC'TCA_GC Cuts at: 2708 2708 Size: 5575 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3766 3800 1903 Size: 34 50 34 1745 34 3678 Fragments arranged by size: 3678 1745 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4677 3467 Size: 1210 4365 BclI T'GATC_A Cuts at: 1548 1548 Size: 5575 BfrBI ATG'CAT Cuts at: 2823 2872 2823 Size: 49 5526 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2670 2840 4186 Size: 107 122 71 631 605 998 170 1346 Cuts at: 4186 136 Size: 1525 Fragments arranged by size: 1525 1346 998 631 605 170 122 107 71 BglII A'GATC_T Cuts at: 5570 5570 Size: 5575 BlpI GC'TnA_GC Cuts at: 2992 2992 Size: 5575 Bme1580I G_kGCm'C Cuts at: 1790 2049 2583 3636 4792 1790 Size: 259 534 1053 1156 2573 Fragments arranged by size: 2573 1156 1053 534 259 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5575 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3306 4264 442 Size: 416 53 83 399 24 1889 958 1753 Fragments arranged by size: 1889 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4235 1239 Size: 2996 2579 Bpu10I CC'TnA_GC Cuts at: 2708 2708 Size: 5575 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3700 4565 4863 3700 Size: 865 298 4412 Fragments arranged by size: 4412 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4238 882 Size: 617 2739 2219 Fragments arranged by size: 2739 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2398 3366 493 Size: 711 1194 968 2702 Fragments arranged by size: 2702 1194 968 711 BsaBI GATnn'nnATC Cuts at: 5569 5569 Size: 5575 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2214 3382 3764 4399 Size: 53 83 186 936 681 1168 382 635 Cuts at: 4399 275 Size: 1451 Fragments arranged by size: 1451 1168 936 681 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2138 4008 4406 4680 4827 5238 2138 Size: 1870 398 274 147 411 2475 Fragments arranged by size: 2475 1870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2447 2477 1178 Size: 30 165 30 1044 30 4276 Fragments arranged by size: 4276 1044 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5575 BseYI C'CCAG_C Cuts at: 2421 2860 4778 5274 2421 Size: 439 1918 496 2722 Fragments arranged by size: 2722 1918 496 439 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 1163 Size: 573 492 4510 Fragments arranged by size: 4510 573 492 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3025 3786 4814 1540 Size: 275 186 1024 761 1028 2301 Fragments arranged by size: 2301 1028 1024 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2136 2172 3636 3721 4792 2136 Size: 36 1464 85 1071 2919 Fragments arranged by size: 2919 1464 1071 85 36 BsmI GAATG_Cn' Cuts at: 3086 3179 3086 Size: 93 5482 BsmBI CGTCTCn'nnnn_ Cuts at: 2731 2731 Size: 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2136 2172 2583 2894 3636 3721 Size: 399 259 87 36 411 311 742 85 Cuts at: 3721 4792 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 311 259 87 85 36 BspEI T'CCGG_A Cuts at: 5238 5238 Size: 5575 BspHI T'CATG_A Cuts at: 3464 5194 3464 Size: 1730 3845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 844 Size: 2086 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3462 1394 Size: 429 1639 3507 Fragments arranged by size: 3507 1639 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4070 4244 65 Size: 1183 2822 174 1396 Fragments arranged by size: 2822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2804 4219 4406 1700 Size: 110 994 1415 187 2869 Fragments arranged by size: 2869 1415 994 187 110 BsrGI T'GTAC_A Cuts at: 96 2751 96 Size: 2655 2920 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5575 BssSI C'ACGA_G Cuts at: 3635 4647 3635 Size: 1012 4563 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5575 BstBI TT'CG_AA Cuts at: 4379 4379 Size: 5575 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5575 BstXI CCAn_nnnn'nTGG Cuts at: 2548 2548 Size: 5575 BstYI r'GATC_y Cuts at: 1972 2322 2853 2905 3272 3657 3674 5126 5212 Size: 350 531 52 367 385 17 1452 86 Cuts at: 5212 5224 5570 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 531 385 367 350 346 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2497 2583 513 Size: 552 1432 86 3505 Fragments arranged by size: 3505 1432 552 86 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4836 Fragments arranged by size: 4836 683 56 BtsI GCAGTG_nn' Cuts at: 2245 3091 3885 3905 2245 Size: 846 794 20 3915 Fragments arranged by size: 3915 846 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5575 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5540 DraI TTT'AAA Cuts at: 2879 2879 Size: 5575 DraIII CAC_nnn'GTG Cuts at: 2296 2411 2296 Size: 115 5460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4582 809 Size: 507 3266 1802 Fragments arranged by size: 3266 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2637 3022 3911 Size: 54 1425 50 275 18 807 385 889 Cuts at: 3911 5540 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 807 385 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3022 1537 Size: 275 1210 4090 Fragments arranged by size: 4090 1210 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2111 2120 2126 3505 1353 Size: 758 9 6 1379 3423 Fragments arranged by size: 3423 1379 758 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4162 4536 4682 387 Size: 1064 138 2573 374 146 1280 Fragments arranged by size: 2573 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2134 2170 2134 Size: 36 5539 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2591 3638 4235 5022 5290 1239 Size: 393 370 589 1047 597 787 268 1524 Fragments arranged by size: 1524 1047 787 597 589 393 370 268 EcoO109I rG'GnC_Cy Cuts at: 2053 2380 2790 2053 Size: 327 410 4838 Fragments arranged by size: 4838 410 327 EcoRI G'AATT_C Cuts at: 2884 3262 2884 Size: 378 5197 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2482 2482 Size: 5575 FspI TGC'GCA Cuts at: 4081 4081 Size: 5575 HaeII r_GCGC'y Cuts at: 1678 4722 1678 Size: 3044 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4216 4248 4290 4322 1373 Size: 32 135 32 2644 32 42 32 2626 Fragments arranged by size: 2644 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2919 3165 669 Size: 988 281 981 246 3079 Fragments arranged by size: 3079 988 981 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5575 HpaI GTT'AAC Cuts at: 3165 3165 Size: 5575 KpnI G_GTAC'C Cuts at: 2900 2900 Size: 5575 MfeI C'AATT_G Cuts at: 3174 3174 Size: 5575 MluI A'CGCG_T Cuts at: 5451 5451 Size: 5575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 4689 4873 1305 Size: 1260 2124 184 2007 Fragments arranged by size: 2124 2007 1260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2639 10 Size: 54 1425 343 807 2946 Fragments arranged by size: 2946 1425 807 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3533 3892 4051 518 Size: 648 2367 359 159 2042 Fragments arranged by size: 2367 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2199 2329 2614 2749 2860 2991 3668 4816 Size: 593 130 285 135 111 131 677 1148 Cuts at: 4816 5061 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 593 285 245 135 131 130 111 NaeI GCC'GGC Cuts at: 2806 2806 Size: 5575 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5023 NdeI CA'TA_TG Cuts at: 387 387 Size: 5575 NgoMIV G'CCGG_C Cuts at: 2804 2804 Size: 5575 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5575 NotI GC'GGCC_GC Cuts at: 3022 3022 Size: 5575 NsiI A_TGCA'T Cuts at: 2825 2874 2825 Size: 49 5526 NspI r_CATG'y Cuts at: 1132 1229 2450 2876 2933 4478 1132 Size: 97 1221 426 57 1545 2229 Fragments arranged by size: 2229 1545 1221 426 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5575 PciI A'CATG_T Cuts at: 4474 4474 Size: 5575 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5575 PfoI T'CCnGG_A Cuts at: 2811 2811 Size: 5575 PmeI GTTT'AAAC Cuts at: 2879 2879 Size: 5575 PmlI CAC'GTG Cuts at: 2398 2398 Size: 5575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2348 2380 3644 3676 5157 5189 2348 Size: 32 1264 32 1481 32 2734 Fragments arranged by size: 2734 1481 1264 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2053 2380 2790 2053 Size: 327 410 4838 Fragments arranged by size: 4838 410 327 PshAI GACnn'nnGTC Cuts at: 1613 2217 2447 1613 Size: 604 230 4741 Fragments arranged by size: 4741 604 230 PsiI TTA'TAA Cuts at: 2147 3145 2147 Size: 998 4577 PstI C_TGCA'G Cuts at: 830 2927 830 Size: 2097 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5575 PvuII CAG'CTG Cuts at: 2860 2860 Size: 5575 SacI G_AGCT'C Cuts at: 2136 2172 2136 Size: 36 5539 SalI G'TCGA_C Cuts at: 1936 2917 1936 Size: 981 4594 SbfI CC_TGCA'GG Cuts at: 2927 2927 Size: 5575 ScaI AGT'ACT Cuts at: 3823 5535 3823 Size: 1712 3863 SfcI C'TryA_G Cuts at: 826 1045 2035 2480 2659 2923 4058 4739 4930 Size: 219 990 445 179 264 1135 681 191 Cuts at: 4930 826 Size: 1471 Fragments arranged by size: 1471 1135 990 681 445 264 219 191 179 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5575 SmaI CCC'GGG Cuts at: 1872 2902 1872 Size: 1030 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3679 4580 4842 820 Size: 197 926 1736 901 262 1553 Fragments arranged by size: 1736 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3366 493 Size: 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5575 SphI G_CATG'C Cuts at: 1132 2933 1132 Size: 1801 3774 SspI AAT'ATT Cuts at: 5 52 3499 5 Size: 47 3447 2081 Fragments arranged by size: 3447 2081 47 StuI AGG'CCT Cuts at: 1517 2843 1517 Size: 1326 4249 StyI C'CwwG_G Cuts at: 513 1065 2049 3014 513 Size: 552 984 965 3074 Fragments arranged by size: 3074 984 965 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3602 3619 3772 3957 4402 325 Size: 1255 2022 17 153 185 445 1498 Fragments arranged by size: 2022 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2628 2727 2751 Size: 274 80 33 51 1443 651 99 24 Cuts at: 2751 3821 5533 96 Size: 1070 1712 138 Fragments arranged by size: 1712 1443 1070 651 274 138 99 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2447 2479 5535 5567 2447 Size: 32 3056 32 2455 Fragments arranged by size: 3056 2455 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5575 XbaI T'CTAG_A Cuts at: 2911 2911 Size: 5575 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5575 XmaI C'CCGG_G Cuts at: 1870 2900 1870 Size: 1030 4545 XmnI GAAnn'nnTTC Cuts at: 3704 3704 Size: 5575 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2215 3383 276 Size: 53 83 186 936 681 1168 2468 Fragments arranged by size: 2468 1168 936 681 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI