Restriction map for
pFN21AA1162
(Linear) (Six-base) MAPSORT of: KIAA1162.seq Check: 6421 from: 1 to: 1047
KIAA1162.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 971 1047
Size: 235 736 76
Fragments arranged by size:
736 235 76
AleI CACnn'nnGTG
Cuts at: 0 991 1047
Size: 991 56
ApaI G_GGCC'C
Cuts at: 0 902 1047
Size: 902 145
ApoI r'AATT_y
Cuts at: 0 172 545 1047
Size: 172 373 502
Fragments arranged by size:
502 373 172
AvaI C'yCGr_G
Cuts at: 0 79 923 1047
Size: 79 844 124
Fragments arranged by size:
844 124 79
BanII G_rGCy'C
Cuts at: 0 902 1047
Size: 902 145
BbsI GAAGACnn'nnnn_
Cuts at: 0 392 1007 1047
Size: 392 615 40
Fragments arranged by size:
615 392 40
BclI T'GATC_A
Cuts at: 0 891 1047
Size: 891 156
BglII A'GATC_T
Cuts at: 0 804 1047
Size: 804 243
Bme1580I G_kGCm'C
Cuts at: 0 902 1047
Size: 902 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1029 1047
Size: 1029 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 1047
Size: 145 30 872
Fragments arranged by size:
872 145 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 712 748 853 856 886 958 1047
Size: 97 615 36 105 3 30 72 89
Fragments arranged by size:
615 105 97 89 72 36 30 3
BseYI C'CCAG_C
Cuts at: 0 324 978 1047
Size: 324 654 69
Fragments arranged by size:
654 324 69
Bsp1286I G_dGCh'C
Cuts at: 0 902 1047
Size: 902 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 1047
Size: 398 649
BstBI TT'CG_AA
Cuts at: 0 383 1047
Size: 383 664
BstYI r'GATC_y
Cuts at: 0 804 1047
Size: 804 243
Bsu36I CC'TnA_GG
Cuts at: 0 941 1047
Size: 941 106
BtgI C'CryG_G
Cuts at: 0 184 1047
Size: 184 863
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 827 1047
Size: 827 220
EaeI y'GGCC_r
Cuts at: 0 41 300 1047
Size: 41 259 747
Fragments arranged by size:
747 259 41
EarI CTCTTCn'nnn_
Cuts at: 0 788 830 1047
Size: 788 42 217
Fragments arranged by size:
788 217 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 346 1047
Size: 346 701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 971 1047
Size: 235 736 76
Fragments arranged by size:
736 235 76
EcoO109I rG'GnC_Cy
Cuts at: 0 76 898 899 1047
Size: 76 822 1 148
Fragments arranged by size:
822 148 76 1
EcoRI G'AATT_C
Cuts at: 0 545 1047
Size: 545 502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 793 823 1047
Size: 793 30 224
Fragments arranged by size:
793 224 30
HaeII r_GCGC'y
Cuts at: 0 15 37 1047
Size: 15 22 1010
Fragments arranged by size:
1010 22 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 498 530 1047
Size: 498 32 517
Fragments arranged by size:
517 498 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1047
Size: 158 889
MslI CAynn'nnrTG
Cuts at: 0 991 1047
Size: 991 56
MspA1I CmG'CkG
Cuts at: 0 982 1047
Size: 982 65
NcoI C'CATG_G
Cuts at: 0 184 1047
Size: 184 863
NspI r_CATG'y
Cuts at: 0 1033 1047
Size: 1033 14
PspOMI G'GGCC_C
Cuts at: 0 898 1047
Size: 898 149
PstI C_TGCA'G
Cuts at: 0 395 1047
Size: 395 652
PvuII CAG'CTG
Cuts at: 0 982 1047
Size: 982 65
SexAI A'CCwGG_T
Cuts at: 0 288 1047
Size: 288 759
SfcI C'TryA_G
Cuts at: 0 391 1047
Size: 391 656
SmaI CCC'GGG
Cuts at: 0 925 1047
Size: 925 122
SmlI C'TyrA_G
Cuts at: 0 1008 1047
Size: 1008 39
SphI G_CATG'C
Cuts at: 0 1033 1047
Size: 1033 14
StyI C'CwwG_G
Cuts at: 0 184 587 655 1047
Size: 184 403 68 392
Fragments arranged by size:
403 392 184 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 145 177 1047
Size: 145 32 870
Fragments arranged by size:
870 145 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 1047
Size: 141 906
XmaI C'CCGG_G
Cuts at: 0 923 1047
Size: 923 124
XmnI GAAnn'nnTTC
Cuts at: 0 248 1047
Size: 248 799
Enzymes that do cut:
AcuI AleI ApaI ApoI AvaI BanII BbsI BclI
BglII Bme1580I BpuEI BsaXI BseRI BseYI Bsp1286I BspMI
BstBI BstYI Bsu36I BtgI BtgZI EaeI EarI EciI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I MmeI MslI
MspA1I NcoI NspI PspOMI PstI PvuII SexAI SfcI
SmaI SmlI SphI StyI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XhoI ZraI