Restriction map for
pFN21AA1139
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AhdI AscI AseI AsnI
Asp718I AspEI AspI AviII BbrPI
BciVI BclI BfrI BsaI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KpnI KspI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 469 GAATTT [GA]^AATT[CT]
AgeI 49 ACCGGT A^CCGGT
Alw44I 1703 GTGCAC G^TGCAC
Aor51HI 861 AGCGCT AGC^GCT
ApaLI 1703 GTGCAC G^TGCAC
ApoI 469 GAATTT [GA]^AATT[CT]
Asp700I 783 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2466 CCTAGG C^CTAGG
BamHI 845 GGATCC G^GATCC
BbeI 1907 GGCGCC GGCGC^C
BbsI 444 GAAGAC GAAGAC(2/6)
BglII 1421 AGATCT A^GATCT
BlnI 2466 CCTAGG C^CTAGG
BpuAI 444 GAAGAC GAAGAC(2/6)
BsaAI 753 TACGTA [CT]AC^GT[GA]
BsmBI 2634 CGTCTC CGTCTC(1/5)
BsrDI 239 GCAATG GCAATG(2/0)
BssHII 306 GCGCGC G^CGCGC
BstAPI 822 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1596 GGTGACC G^GT[ACGT]ACC
BstPI 1596 GGTGACC G^GT[ACGT]ACC
DraIII 2717 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3450 GACCAGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3489 GAAGAG CTCTTC(1/4)
Eco47III 861 AGCGCT AGC^GCT
EcoO65I 1596 GGTGACC G^GT[ACGT]ACC
HindIII 2221 AAGCTT A^AGCTT
KasI 1907 GGCGCC G^GCGCC
Ksp632I 3489 GAAGAG CTCTTC(1/4)
MseI 2969 TTAA T^TAA
NarI 1907 GGCGCC GG^CGCC
NheI 3354 GCTAGC G^CTAGC
NspI 245 GCATGC [GA]CATG^[CT]
PinAI 49 ACCGGT A^CCGGT
SapI 3489 GAAGAGC GCTCTTC(1/4)
SexAI 1337 ACCAGGT A^CC[AT]GGT
SfoI 1907 GGCGCC GGC^GCC
SmaI 1014 CCCGGG CCC^GGG
SnaBI 753 TACGTA TAC^GTA
SphI 245 GCATGC GCATG^C
StuI 836 AGGCCT AGG^CCT
Tru9I 2969 TTAA T^TAA
Tsp509I 470 AATT ^AATT
XmaCI 1014 CCCGGG C^CCGGG
XmaI 1014 CCCGGG C^CCGGG
XmnI 783 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BcgI 888 CGATCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3560 GCACCATGTTCG
BsaBI 636 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1935 GATGGCCATC
BsiEI 951 CGGCCG CG[GA][CT]^CG
3257 CGGCCG
BssSI 676 CACGAG CACGAG(-5/-1)
973 CACGAG
BstXI 1281 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1757 CCATGGGGCTGG
EagI 951 CGGCCG C^GGCCG
3257 CGGCCG
EclXI 951 CGGCCG C^GGCCG
3257 CGGCCG
Eco52I 951 CGGCCG C^GGCCG
3257 CGGCCG
MamI 636 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1935 GATGGCCATC
SacI 187 GAGCTC GAGCT^C
1260 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII