(Circular) (Six-base) MAPSORT of: pFN21AA1139.seq Check: 6710 from: 1 to: 8311 pFN21AA1139 8311 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3188 4087 4771 3188 Size: 899 684 6728 Fragments arranged by size: 6728 899 684 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6121 278 Size: 53 83 186 936 4585 2468 Fragments arranged by size: 4585 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5654 1937 Size: 3717 4594 Acc65I G'GTAC_C Cuts at: 5632 5632 Size: 8311 AclI AA'CG_TT Cuts at: 1563 6438 6811 1563 Size: 4875 373 3063 Fragments arranged by size: 4875 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2876 6374 7758 8026 1632 Size: 1244 3498 1384 268 1917 Fragments arranged by size: 3498 1917 1384 1244 268 AfeI AGC'GCT Cuts at: 2868 2868 Size: 8311 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8114 AflIII A'CryG_T Cuts at: 1203 7210 8187 1203 Size: 6007 977 1327 Fragments arranged by size: 6007 1327 977 AgeI A'CCGG_T Cuts at: 2054 7142 2054 Size: 5088 3223 AhdI GACnn_n'nnGTC Cuts at: 7040 7040 Size: 8311 AleI CACnn'nnGTG Cuts at: 1166 2520 1166 Size: 1354 6957 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2267 2385 2607 3141 1184 Size: 1083 118 222 534 6354 Fragments arranged by size: 6354 1083 534 222 118 AlwNI CAG_nnn'CTG Cuts at: 2023 2068 2629 2833 3322 3349 3460 4811 4970 Size: 45 561 204 489 27 111 1351 159 Cuts at: 4970 5446 6054 7626 2023 Size: 476 608 1572 2708 Fragments arranged by size: 2708 1572 1351 608 561 489 476 204 159 111 45 27 ApaI G_GGCC'C Cuts at: 4356 4594 4689 4710 4779 5426 4356 Size: 238 95 21 69 647 7241 Fragments arranged by size: 7241 647 238 95 69 21 ApaLI G'TGCA_C Cuts at: 3708 6368 7524 3708 Size: 2660 1156 4495 Fragments arranged by size: 4495 2660 1156 ApoI r'AATT_y Cuts at: 1493 2474 5620 5846 5998 8107 1493 Size: 981 3146 226 152 2109 1697 Fragments arranged by size: 3146 2109 1697 981 226 152 AseI AT'TA_AT Cuts at: 160 1032 6865 160 Size: 872 5833 1606 Fragments arranged by size: 5833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8311 AvaI C'yCGr_G Cuts at: 1870 1943 3019 3168 3821 4554 4830 4917 5120 Size: 73 1076 149 653 733 276 87 203 Cuts at: 5120 5636 1870 Size: 516 4545 Fragments arranged by size: 4545 1076 733 653 516 276 203 149 87 73 AvrII C'CTAG_G Cuts at: 4471 4471 Size: 8311 BamHI G'GATC_C Cuts at: 2850 5641 6008 2850 Size: 2791 367 5153 Fragments arranged by size: 5153 2791 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3912 5287 5504 5522 5632 Size: 325 217 627 2125 1375 217 18 110 Cuts at: 5632 7087 618 Size: 1455 1842 Fragments arranged by size: 2125 1842 1455 1375 627 325 217 217 110 18 BanII G_rGCy'C Cuts at: 1391 2111 2196 3269 4289 4356 4594 4682 4689 Size: 720 85 1073 1020 67 238 88 7 Cuts at: 4689 4710 4752 4779 5127 5426 5630 1391 Size: 21 42 27 348 299 204 4072 Fragments arranged by size: 4072 1073 1020 720 348 299 238 204 88 85 67 42 27 21 7 BbeI G_GCGC'C Cuts at: 3916 3916 Size: 8311 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2456 928 Size: 971 557 6783 Fragments arranged by size: 6783 971 557 BbvCI CC'TCA_GC Cuts at: 3179 3899 4437 4631 3179 Size: 720 538 194 6859 Fragments arranged by size: 6859 720 538 194 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2882 2916 5554 5588 6502 6536 1903 Size: 34 945 34 2638 34 914 34 3678 Fragments arranged by size: 3678 2638 945 914 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6203 7413 6203 Size: 1210 7101 BclI T'GATC_A Cuts at: 1548 1548 Size: 8311 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2299 2321 2991 Size: 107 122 71 631 605 627 22 670 Cuts at: 2991 4416 4589 4714 6922 136 Size: 1425 173 125 2208 1525 Fragments arranged by size: 2208 1525 1425 670 631 627 605 173 125 122 107 71 22 BglII A'GATC_T Cuts at: 3426 8306 3426 Size: 4880 3431 BlpI GC'TnA_GC Cuts at: 3497 3645 3650 5224 5728 3497 Size: 148 5 1574 504 6080 Fragments arranged by size: 6080 1574 504 148 5 Bme1580I G_kGCm'C Cuts at: 1790 2996 3278 3618 3712 4114 4211 4356 4516 Size: 1206 282 340 94 402 97 145 160 Cuts at: 4516 4594 4689 4710 4779 4935 5292 5426 5525 Size: 78 95 21 69 156 357 134 99 Cuts at: 5525 6372 7528 1790 Size: 847 1156 2573 Fragments arranged by size: 2573 1206 1156 847 402 357 340 282 160 156 145 134 99 97 95 94 78 69 21 BmgBI CAC'GTC Cuts at: 1318 2743 3442 1318 Size: 1425 699 6187 Fragments arranged by size: 6187 1425 699 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3722 5378 5421 Size: 416 53 83 399 24 2305 1656 43 Cuts at: 5421 6042 7000 442 Size: 621 958 1753 Fragments arranged by size: 2305 1753 1656 958 621 416 399 83 53 43 24 BmtI G_CTAG'C Cuts at: 1056 5363 1056 Size: 4307 4004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2309 2393 2555 2747 3354 3482 3533 Size: 971 99 84 162 192 607 128 51 Cuts at: 3533 3716 3977 5082 5172 5468 6971 1239 Size: 183 261 1105 90 296 1503 2579 Fragments arranged by size: 2579 1503 1105 971 607 296 261 192 183 162 128 99 90 84 51 Bpu10I CC'TnA_GC Cuts at: 2217 3179 3386 3899 4224 4386 4406 4437 4631 Size: 962 207 513 325 162 20 31 194 Cuts at: 4631 5355 2217 Size: 724 5173 Fragments arranged by size: 5173 962 724 513 325 207 194 162 31 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 2855 5267 6436 7301 7599 2471 Size: 384 2412 1169 865 298 3183 Fragments arranged by size: 3183 2412 1169 865 384 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6974 882 Size: 617 5475 2219 Fragments arranged by size: 5475 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2760 6102 493 Size: 711 1556 3342 2702 Fragments arranged by size: 3342 2702 1556 711 BsaBI GATnn'nnATC Cuts at: 2645 3944 8305 2645 Size: 1299 4361 2651 Fragments arranged by size: 4361 2651 1299 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2453 3913 4820 5577 Size: 53 83 186 936 920 1460 907 757 Cuts at: 5577 5598 6118 6500 7135 275 Size: 21 520 382 635 1451 Fragments arranged by size: 1460 1451 936 920 907 757 635 520 382 186 83 53 21 BsaWI w'CCGG_w Cuts at: 2054 2751 3931 4521 6744 7142 7416 7563 7974 Size: 697 1180 590 2223 398 274 147 411 Cuts at: 7974 2054 Size: 2391 Fragments arranged by size: 2391 2223 1180 697 590 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3689 3719 4056 4086 1178 Size: 30 165 30 2286 30 337 30 5403 Fragments arranged by size: 5403 2286 337 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 3806 3986 4554 4808 4811 4814 5085 Size: 1210 1410 180 568 254 3 3 271 Cuts at: 5085 5270 5382 1186 Size: 185 112 4115 Fragments arranged by size: 4115 1410 1210 568 271 254 185 180 112 3 3 BseYI C'CCAG_C Cuts at: 2496 2636 3193 3315 3671 3844 3973 4091 4160 Size: 140 557 122 356 173 129 118 69 Cuts at: 4160 4175 4549 4649 4772 5115 5126 5168 5334 Size: 15 374 100 123 343 11 42 166 Cuts at: 5334 5436 5528 7514 8010 2496 Size: 102 92 1986 496 2797 Fragments arranged by size: 2797 1986 557 496 374 356 343 173 166 140 129 123 122 118 102 100 92 69 42 15 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2246 2345 2355 2567 5314 5460 Size: 573 345 165 99 10 212 2747 146 Cuts at: 5460 1163 Size: 4014 Fragments arranged by size: 4014 2747 573 345 212 165 146 99 10 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2959 5265 5761 6522 7550 1540 Size: 275 186 958 2306 496 761 1028 2301 Fragments arranged by size: 2306 2301 1028 958 761 496 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 3269 3712 5411 6372 6457 7528 2196 Size: 1073 443 1699 961 85 1071 2979 Fragments arranged by size: 2979 1699 1073 1071 961 443 85 BsmI GAATG_Cn' Cuts at: 5822 5915 5822 Size: 93 8218 BsmBI CGTCTCn'nnnn_ Cuts at: 4645 4645 Size: 8311 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 2196 2996 3269 3278 3618 3712 Size: 399 321 85 800 273 9 340 94 Cuts at: 3712 4114 4211 4289 4356 4516 4594 4682 4689 Size: 402 97 78 67 160 78 88 7 Cuts at: 4689 4710 4752 4779 4935 5127 5292 5411 5426 Size: 21 42 27 156 192 165 119 15 Cuts at: 5426 5525 5630 6372 6457 7528 1391 Size: 99 105 742 85 1071 2174 Fragments arranged by size: 2174 1071 800 742 402 399 340 321 273 192 165 160 156 119 105 99 97 94 88 85 85 78 78 67 42 27 21 15 9 7 BspEI T'CCGG_A Cuts at: 2751 3931 4521 7974 2751 Size: 1180 590 3453 3088 Fragments arranged by size: 3453 3088 1180 590 BspHI T'CATG_A Cuts at: 6200 7930 6200 Size: 1730 6581 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3188 3699 4087 4741 4771 5666 844 Size: 2344 511 388 654 30 895 3489 Fragments arranged by size: 3489 2344 895 654 511 388 30 BsrBI CCG'CTC Cuts at: 1394 1823 3414 4086 4216 6198 1394 Size: 429 1591 672 130 1982 3507 Fragments arranged by size: 3507 1982 1591 672 429 130 BsrDI GCAATG_nn' Cuts at: 65 1248 2251 6806 6980 65 Size: 1183 1003 4555 174 1396 Fragments arranged by size: 4555 1396 1183 1003 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 2283 3251 3542 3915 4392 6955 Size: 110 244 229 968 291 373 477 2563 Cuts at: 6955 7142 1700 Size: 187 2869 Fragments arranged by size: 2869 2563 968 477 373 291 244 229 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8311 BssHII G'CGCG_C Cuts at: 1925 2311 1925 Size: 386 7925 BssSI C'ACGA_G Cuts at: 2681 2978 6371 7383 2681 Size: 297 3393 1012 3609 Fragments arranged by size: 3609 3393 1012 297 BstAPI GCAn_nnn'nTGC Cuts at: 794 2833 794 Size: 2039 6272 BstBI TT'CG_AA Cuts at: 7115 7115 Size: 8311 BstEII G'GTnAC_C Cuts at: 1184 3601 1184 Size: 2417 5894 BstXI CCAn_nnnn'nTGG Cuts at: 3293 3769 3293 Size: 476 7835 BstYI r'GATC_y Cuts at: 1972 2352 2850 3426 4171 5641 6008 6393 6410 Size: 380 498 576 745 1470 367 385 17 Cuts at: 6410 7862 7948 7960 8306 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1470 1452 745 576 498 385 380 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2845 3579 4812 2845 Size: 734 1233 6344 Fragments arranged by size: 6344 1233 734 BtgI C'CryG_G Cuts at: 513 1065 2004 3286 3762 3989 4041 5396 513 Size: 552 939 1282 476 227 52 1355 3428 Fragments arranged by size: 3428 1355 1282 939 552 476 227 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3640 487 Size: 683 56 2414 5158 Fragments arranged by size: 5158 2414 683 56 BtsI GCAGTG_nn' Cuts at: 2528 2898 3226 3253 3320 3522 4516 5006 5018 Size: 370 328 27 67 202 994 490 12 Cuts at: 5018 5066 5827 6621 6641 2528 Size: 48 761 794 20 4198 Fragments arranged by size: 4198 994 794 761 490 370 328 202 67 48 27 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8276 DraI TTT'AAA Cuts at: 5615 5615 Size: 8311 DraIII CAC_nnn'GTG Cuts at: 4727 4727 Size: 8311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5461 7318 809 Size: 507 4145 1857 1802 Fragments arranged by size: 4145 1857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2479 2602 2956 Size: 54 1425 50 275 18 649 123 354 Cuts at: 2956 3283 3402 3446 3591 3774 3921 3942 4038 Size: 327 119 44 145 183 147 21 96 Cuts at: 4038 4129 4246 4347 4499 5037 5262 5758 6647 Size: 91 117 101 152 538 225 496 889 Cuts at: 6647 8276 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 649 538 496 354 327 275 225 183 152 147 145 123 119 117 101 96 91 54 50 44 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2956 5262 5758 1537 Size: 275 1144 2306 496 4090 Fragments arranged by size: 4090 2306 1144 496 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5489 6241 1353 Size: 4136 752 3423 Fragments arranged by size: 4136 3423 752 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3878 6898 7272 7418 387 Size: 1064 138 2289 3020 374 146 1280 Fragments arranged by size: 3020 2289 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2194 3267 2194 Size: 1073 7238 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2309 2393 2555 2747 2876 3354 Size: 393 578 99 84 162 192 129 478 Cuts at: 3354 3482 3533 3716 3977 5082 5172 5468 6374 Size: 128 51 183 261 1105 90 296 906 Cuts at: 6374 6971 7758 8026 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1105 906 787 597 578 478 393 296 268 261 192 183 162 129 128 99 90 84 51 EcoNI CCTnn'n_nnAGG Cuts at: 3884 4202 4248 5282 3884 Size: 318 46 1034 6913 Fragments arranged by size: 6913 1034 318 46 EcoO109I rG'GnC_Cy Cuts at: 2020 3583 3877 3895 4253 4353 4604 4685 4706 Size: 1563 294 18 358 100 251 81 21 Cuts at: 4706 4707 4808 2020 Size: 1 101 5523 Fragments arranged by size: 5523 1563 358 294 251 101 100 81 21 18 1 EcoRI G'AATT_C Cuts at: 5620 5998 5620 Size: 378 7933 FspI TGC'GCA Cuts at: 6817 6817 Size: 8311 HaeII r_GCGC'y Cuts at: 1678 2678 2777 2870 3916 4393 4630 4894 5366 Size: 1000 99 93 1046 477 237 264 472 Cuts at: 5366 5535 7458 1678 Size: 169 1923 2531 Fragments arranged by size: 2531 1923 1046 1000 477 472 264 237 169 99 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2601 2625 2633 Size: 32 135 32 566 32 431 24 8 Cuts at: 2633 2657 6952 6984 7026 7058 1373 Size: 24 4295 32 42 32 2626 Fragments arranged by size: 4295 2626 566 431 135 42 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2230 2437 3814 5655 5901 669 Size: 988 281 292 207 1377 1841 246 3079 Fragments arranged by size: 3079 1841 1377 988 292 281 246 207 HindIII A'AGCT_T Cuts at: 748 4226 748 Size: 3478 4833 HpaI GTT'AAC Cuts at: 5901 5901 Size: 8311 KasI G'GCGC_C Cuts at: 3912 3912 Size: 8311 KpnI G_GTAC'C Cuts at: 5636 5636 Size: 8311 MfeI C'AATT_G Cuts at: 5910 5910 Size: 8311 MluI A'CGCG_T Cuts at: 8187 8187 Size: 8311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2445 5177 7425 7609 1305 Size: 1140 2732 2248 184 2007 Fragments arranged by size: 2732 2248 2007 1140 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2604 3285 3404 3923 3944 Size: 54 1425 343 772 681 119 519 21 Cuts at: 3944 4131 4248 5039 10 Size: 187 117 791 3282 Fragments arranged by size: 3282 1425 791 772 681 519 343 187 119 117 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2520 2933 3599 6269 6628 6787 518 Size: 648 1354 413 666 2670 359 159 2042 Fragments arranged by size: 2670 2042 1354 666 648 413 359 159 MspA1I CmG'CkG Cuts at: 1606 2260 2500 2961 3258 3457 3511 3739 3750 Size: 654 240 461 297 199 54 228 11 Cuts at: 3750 3983 4164 4904 5060 5338 5474 5593 5727 Size: 233 181 740 156 278 136 119 134 Cuts at: 5727 6404 7552 7797 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 740 677 654 461 297 278 245 240 233 228 199 181 156 136 134 119 54 11 NaeI GCC'GGC Cuts at: 2285 3253 3544 3917 2285 Size: 968 291 373 6679 Fragments arranged by size: 6679 968 373 291 NarI GG'CG_CC Cuts at: 3913 3913 Size: 8311 NcoI C'CATG_G Cuts at: 513 1065 2004 3286 3762 4041 513 Size: 552 939 1282 476 279 4783 Fragments arranged by size: 4783 1282 939 552 476 279 NdeI CA'TA_TG Cuts at: 387 387 Size: 8311 NgoMIV G'CCGG_C Cuts at: 2283 3251 3542 3915 2283 Size: 968 291 373 6679 Fragments arranged by size: 6679 968 373 291 NheI G'CTAG_C Cuts at: 1052 5359 1052 Size: 4307 4004 NotI GC'GGCC_GC Cuts at: 5758 5758 Size: 8311 NspI r_CATG'y Cuts at: 1132 1229 2254 5669 7214 1132 Size: 97 1025 3415 1545 2229 Fragments arranged by size: 3415 2229 1545 1025 97 PasI CC'CwG_GG Cuts at: 1348 4282 4307 4329 4682 5204 1348 Size: 2934 25 22 353 522 4455 Fragments arranged by size: 4455 2934 522 353 25 22 PciI A'CATG_T Cuts at: 7210 7210 Size: 8311 PflMI CCAn_nnn'nTGG Cuts at: 1108 4319 4673 5084 1108 Size: 3211 354 411 4335 Fragments arranged by size: 4335 3211 411 354 PfoI T'CCnGG_A Cuts at: 3107 3107 Size: 8311 PmeI GTTT'AAAC Cuts at: 5615 5615 Size: 8311 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6380 6412 7893 7925 6380 Size: 32 1481 32 6766 Fragments arranged by size: 6766 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 3583 4253 2020 Size: 1563 670 6078 Fragments arranged by size: 6078 1563 670 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8311 PsiI TTA'TAA Cuts at: 5881 5881 Size: 8311 PspOMI G'GGCC_C Cuts at: 4352 4590 4685 4706 4775 5422 4352 Size: 238 95 21 69 647 7241 Fragments arranged by size: 7241 647 238 95 69 21 PstI C_TGCA'G Cuts at: 830 3064 3540 3801 3888 5013 5663 830 Size: 2234 476 261 87 1125 650 3478 Fragments arranged by size: 3478 2234 1125 650 476 261 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8311 PvuII CAG'CTG Cuts at: 2500 3457 3511 3739 3983 5338 5474 5593 2500 Size: 957 54 228 244 1355 136 119 5218 Fragments arranged by size: 5218 1355 957 244 228 136 119 54 SacI G_AGCT'C Cuts at: 2196 3269 2196 Size: 1073 7238 SalI G'TCGA_C Cuts at: 1936 5653 1936 Size: 3717 4594 SapI GCTCTTCn'nnn_ Cuts at: 5489 5489 Size: 8311 SbfI CC_TGCA'GG Cuts at: 5663 5663 Size: 8311 ScaI AGT'ACT Cuts at: 6559 8271 6559 Size: 1712 6599 SexAI A'CCwGG_T Cuts at: 3342 3342 Size: 8311 SfcI C'TryA_G Cuts at: 826 1045 3060 3154 3536 3797 3884 3978 4031 Size: 219 2015 94 382 261 87 94 53 Cuts at: 4031 5009 5659 6794 7475 7666 826 Size: 978 650 1135 681 191 1471 Fragments arranged by size: 2015 1471 1135 978 681 650 382 261 219 191 94 94 87 53 SfoI GGC'GCC Cuts at: 3914 3914 Size: 8311 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8311 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8311 SmaI CCC'GGG Cuts at: 1872 3021 5638 1872 Size: 1149 2617 4545 Fragments arranged by size: 4545 2617 1149 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 2870 5282 6415 7316 7578 Size: 197 926 543 384 2412 1133 901 262 Cuts at: 7578 820 Size: 1553 Fragments arranged by size: 2412 1553 1133 926 901 543 384 262 197 SnaBI TAC'GTA Cuts at: 493 2760 6102 493 Size: 2267 3342 2702 Fragments arranged by size: 3342 2702 2267 SpeI A'CTAG_T Cuts at: 152 152 Size: 8311 SphI G_CATG'C Cuts at: 1132 2254 5669 1132 Size: 1122 3415 3774 Fragments arranged by size: 3774 3415 1122 SspI AAT'ATT Cuts at: 5 52 6235 5 Size: 47 6183 2081 Fragments arranged by size: 6183 2081 47 StuI AGG'CCT Cuts at: 1517 2843 1517 Size: 1326 6985 StyI C'CwwG_G Cuts at: 513 1065 2004 3286 3762 3924 4041 4249 4471 Size: 552 939 1282 476 162 117 208 222 Cuts at: 4471 4689 5143 5508 5750 513 Size: 218 454 365 242 3074 Fragments arranged by size: 3074 1282 939 552 476 454 365 242 222 218 208 162 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2720 3097 3449 5348 6338 6355 6508 Size: 1255 1140 377 352 1899 990 17 153 Cuts at: 6508 6693 7138 325 Size: 185 445 1498 Fragments arranged by size: 1899 1498 1255 1140 990 445 377 352 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6557 8269 96 Size: 274 80 33 51 1443 4580 1712 138 Fragments arranged by size: 4580 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3353 3385 3689 3721 4056 4088 8271 8303 3353 Size: 32 304 32 335 32 4183 32 3361 Fragments arranged by size: 4183 3361 335 304 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8311 XbaI T'CTAG_A Cuts at: 5647 5647 Size: 8311 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2144 2428 3769 3952 5464 5548 2144 Size: 284 1341 183 1512 84 4907 Fragments arranged by size: 4907 1512 1341 284 183 84 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8311 XmaI C'CCGG_G Cuts at: 1870 3019 5636 1870 Size: 1149 2617 4545 Fragments arranged by size: 4545 2617 1149 XmnI GAAnn'nnTTC Cuts at: 2792 6440 2792 Size: 3648 4663 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6119 276 Size: 53 83 186 936 4585 2468 Fragments arranged by size: 4585 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI