(Circular) (Six-base) MAPSORT of: pFN21AA1091.seq Check: 341 from: 1 to: 8566 pFN21AA1091 8566 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4137 4137 Size: 8566 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5843 6376 278 Size: 53 83 186 936 4307 533 2468 Fragments arranged by size: 4307 2468 936 533 186 83 53 AccI GT'mk_AC Cuts at: 1937 5909 1937 Size: 3972 4594 Acc65I G'GTAC_C Cuts at: 5887 5887 Size: 8566 AclI AA'CG_TT Cuts at: 1563 6693 7066 1563 Size: 5130 373 3063 Fragments arranged by size: 5130 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2202 3291 3329 4289 4727 6629 8013 8281 Size: 570 1089 38 960 438 1902 1384 268 Cuts at: 8281 1632 Size: 1917 Fragments arranged by size: 1917 1902 1384 1089 960 570 438 268 38 AfeI AGC'GCT Cuts at: 2573 4754 2573 Size: 2181 6385 AflII C'TTAA_G Cuts at: 820 1017 3392 820 Size: 197 2375 5994 Fragments arranged by size: 5994 2375 197 AflIII A'CryG_T Cuts at: 1203 5742 7465 8442 1203 Size: 4539 1723 977 1327 Fragments arranged by size: 4539 1723 1327 977 AgeI A'CCGG_T Cuts at: 3114 7397 3114 Size: 4283 4283 AhdI GACnn_n'nnGTC Cuts at: 2107 7295 2107 Size: 5188 3378 AleI CACnn'nnGTG Cuts at: 1166 2555 4361 1166 Size: 1389 1806 5371 Fragments arranged by size: 5371 1806 1389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2468 1184 Size: 1284 7282 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5439 5471 5439 Size: 32 8534 AlwNI CAG_nnn'CTG Cuts at: 2965 5665 6309 7881 2965 Size: 2700 644 1572 3650 Fragments arranged by size: 3650 2700 1572 644 ApaLI G'TGCA_C Cuts at: 6623 7779 6623 Size: 1156 7410 ApoI r'AATT_y Cuts at: 1493 2191 3261 5875 6101 6253 8362 1493 Size: 698 1070 2614 226 152 2109 1697 Fragments arranged by size: 2614 2109 1697 1070 698 226 152 AseI AT'TA_AT Cuts at: 160 1032 7120 160 Size: 872 6088 1606 Fragments arranged by size: 6088 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8566 AvaI C'yCGr_G Cuts at: 1870 1943 2882 3822 5891 1870 Size: 73 939 940 2069 4545 Fragments arranged by size: 4545 2069 940 939 73 BamHI G'GATC_C Cuts at: 5181 5896 6263 5181 Size: 715 367 7484 Fragments arranged by size: 7484 715 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2153 2755 5887 7342 Size: 325 217 627 248 118 602 3132 1455 Cuts at: 7342 618 Size: 1842 Fragments arranged by size: 3132 1842 1455 627 602 325 248 217 118 BanII G_rGCy'C Cuts at: 1391 2033 2349 3705 4734 4910 5590 5885 1391 Size: 642 316 1356 1029 176 680 295 4072 Fragments arranged by size: 4072 1356 1029 680 642 316 295 176 BbeI G_GCGC'C Cuts at: 2039 2039 Size: 8566 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2553 3480 4840 5488 928 Size: 971 654 927 1360 648 4006 Fragments arranged by size: 4006 1360 971 927 654 648 BbvCI CC'TCA_GC Cuts at: 2703 2703 Size: 8566 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5845 5879 6757 6791 1903 Size: 34 3908 34 878 34 3678 Fragments arranged by size: 3908 3678 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6458 7668 6458 Size: 1210 7356 BclI T'GATC_A Cuts at: 1548 5763 1548 Size: 4215 4351 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2311 5717 7177 Size: 107 122 71 631 605 639 3406 1460 Cuts at: 7177 136 Size: 1525 Fragments arranged by size: 3406 1525 1460 639 631 605 122 107 71 BglII A'GATC_T Cuts at: 2439 8561 2439 Size: 6122 2444 BlpI GC'TnA_GC Cuts at: 5983 5983 Size: 8566 Bme1580I G_kGCm'C Cuts at: 1790 2920 6627 7783 1790 Size: 1130 3707 1156 2573 Fragments arranged by size: 3707 2573 1156 1130 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8566 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2034 3450 4053 Size: 416 53 83 399 24 617 1416 603 Cuts at: 4053 4081 5256 5328 6297 7255 442 Size: 28 1175 72 969 958 1753 Fragments arranged by size: 1753 1416 1175 969 958 617 603 416 399 83 72 53 28 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8566 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2401 3316 3352 5708 2401 Size: 915 36 2356 5259 Fragments arranged by size: 5259 2356 915 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2945 3146 3752 4435 5195 5855 7226 Size: 875 831 201 606 683 760 660 1371 Cuts at: 7226 1239 Size: 2579 Fragments arranged by size: 2579 1371 875 831 760 683 660 606 201 Bpu10I CC'TnA_GC Cuts at: 2703 3699 4017 2703 Size: 996 318 7252 Fragments arranged by size: 7252 996 318 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2744 3120 3706 6691 7556 7854 2744 Size: 376 586 2985 865 298 3456 Fragments arranged by size: 3456 2985 865 586 376 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2078 2514 2815 3728 7229 882 Size: 617 579 436 301 913 3501 2219 Fragments arranged by size: 3501 2219 913 617 579 436 301 BsaAI yAC'GTr Cuts at: 493 1204 6357 493 Size: 711 5153 2702 Fragments arranged by size: 5153 2702 711 BsaBI GATnn'nnATC Cuts at: 5470 8560 5470 Size: 3090 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 5840 6373 6755 Size: 53 83 186 936 503 3804 533 382 Cuts at: 6755 7390 275 Size: 635 1451 Fragments arranged by size: 3804 1451 936 635 533 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3114 5271 6999 7397 7671 7818 8229 3114 Size: 2157 1728 398 274 147 411 3451 Fragments arranged by size: 3451 2157 1728 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2882 2912 3849 3879 1178 Size: 30 165 30 1479 30 937 30 5865 Fragments arranged by size: 5865 1479 937 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4706 1186 Size: 3520 5046 BseYI C'CCAG_C Cuts at: 3040 3479 3617 4900 5208 7769 8265 3040 Size: 439 138 1283 308 2561 496 3341 Fragments arranged by size: 3341 2561 1283 496 439 308 138 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5721 1163 Size: 573 3985 4008 Fragments arranged by size: 4008 3985 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6016 6777 7805 1540 Size: 275 186 4015 761 1028 2301 Fragments arranged by size: 4015 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4734 6627 6712 7783 4734 Size: 1893 85 1071 5517 Fragments arranged by size: 5517 1893 1071 85 BsmI GAATG_Cn' Cuts at: 2298 2318 3094 3376 4081 4280 6077 6170 2298 Size: 20 776 282 705 199 1797 93 4694 Fragments arranged by size: 4694 1797 776 705 282 199 93 20 BsmBI CGTCTCn'nnnn_ Cuts at: 2617 2975 5831 2617 Size: 358 2856 5352 Fragments arranged by size: 5352 2856 358 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2349 2920 3705 4734 4910 5590 Size: 399 243 316 571 785 1029 176 680 Cuts at: 5590 5885 6627 6712 7783 1391 Size: 295 742 85 1071 2174 Fragments arranged by size: 2174 1071 1029 785 742 680 571 399 316 295 243 176 85 BspEI T'CCGG_A Cuts at: 5271 8229 5271 Size: 2958 5608 BspHI T'CATG_A Cuts at: 2649 3778 6455 8185 2649 Size: 1129 2677 1730 3030 Fragments arranged by size: 3030 2677 1730 1129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3717 4137 5921 844 Size: 2873 420 1784 3489 Fragments arranged by size: 3489 2873 1784 420 BsrBI CCG'CTC Cuts at: 1394 1823 2761 5179 6453 1394 Size: 429 938 2418 1274 3507 Fragments arranged by size: 3507 2418 1274 938 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2457 3283 3357 5810 7061 7235 65 Size: 1183 1209 826 74 2453 1251 174 1396 Fragments arranged by size: 2453 1396 1251 1209 1183 826 174 74 BsrFI r'CCGG_y Cuts at: 1700 1810 2775 3114 4485 5139 7210 7397 1700 Size: 110 965 339 1371 654 2071 187 2869 Fragments arranged by size: 2869 2071 1371 965 654 339 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8566 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8566 BssSI C'ACGA_G Cuts at: 6626 7638 6626 Size: 1012 7554 BstAPI GCAn_nnn'nTGC Cuts at: 794 5812 794 Size: 5018 3548 BstBI TT'CG_AA Cuts at: 7370 7370 Size: 8566 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8566 BstXI CCAn_nnnn'nTGG Cuts at: 3106 3217 4180 5077 3106 Size: 111 963 897 6595 Fragments arranged by size: 6595 963 897 111 BstYI r'GATC_y Cuts at: 1972 2439 3493 3549 4410 4587 4932 5181 5325 Size: 467 1054 56 861 177 345 249 144 Cuts at: 5325 5896 6263 6648 6665 8117 8203 8215 8561 Size: 571 367 385 17 1452 86 12 346 Cuts at: 8561 1972 Size: 1977 Fragments arranged by size: 1977 1452 1054 861 571 467 385 367 346 345 249 177 144 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 4522 4583 5214 5565 4522 Size: 61 631 351 7523 Fragments arranged by size: 7523 631 351 61 BtgI C'CryG_G Cuts at: 513 1065 4677 4928 513 Size: 552 3612 251 4151 Fragments arranged by size: 4151 3612 552 251 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7827 Fragments arranged by size: 7827 683 56 BtsI GCAGTG_nn' Cuts at: 2452 2714 3295 4917 5610 5733 6082 6876 6896 Size: 262 581 1622 693 123 349 794 20 Cuts at: 6896 2452 Size: 4122 Fragments arranged by size: 4122 1622 794 693 581 349 262 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8566 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8531 DraI TTT'AAA Cuts at: 5470 5870 5470 Size: 400 8166 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2060 2858 7573 809 Size: 507 744 798 4715 1802 Fragments arranged by size: 4715 1802 798 744 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2773 5044 5137 Size: 54 1425 50 275 18 943 2271 93 Cuts at: 5137 6013 6902 8531 8 Size: 876 889 1629 43 Fragments arranged by size: 2271 1629 1425 943 889 876 275 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6013 1537 Size: 275 4201 4090 Fragments arranged by size: 4201 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2415 3305 4362 5489 6496 1353 Size: 1062 890 1057 1127 1007 3423 Fragments arranged by size: 3423 1127 1062 1057 1007 890 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2037 2993 4111 4184 7153 7527 Size: 1064 138 448 956 1118 73 2969 374 Cuts at: 7527 7673 387 Size: 146 1280 Fragments arranged by size: 2969 1280 1118 1064 956 448 374 146 138 73 EcoICRI GAG'CTC Cuts at: 4732 4732 Size: 8566 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2202 2945 3146 3291 3329 3752 Size: 393 482 88 743 201 145 38 423 Cuts at: 3752 4289 4435 4727 5195 5855 6629 7226 8013 Size: 537 146 292 468 660 774 597 787 Cuts at: 8013 8281 1239 Size: 268 1524 Fragments arranged by size: 1524 787 774 743 660 597 537 482 468 423 393 292 268 201 146 145 88 38 EcoNI CCTnn'n_nnAGG Cuts at: 4268 4598 5849 4268 Size: 330 1251 6985 Fragments arranged by size: 6985 1251 330 EcoO109I rG'GnC_Cy Cuts at: 2233 4261 2233 Size: 2028 6538 EcoRI G'AATT_C Cuts at: 3261 5875 6253 3261 Size: 2614 378 5574 Fragments arranged by size: 5574 2614 378 EcoRV GAT'ATC Cuts at: 2246 2246 Size: 8566 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3296 3296 Size: 8566 FspI TGC'GCA Cuts at: 7072 7072 Size: 8566 HaeII r_GCGC'y Cuts at: 1678 2039 2575 4756 7713 1678 Size: 361 536 2181 2957 2531 Fragments arranged by size: 2957 2531 2181 536 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2977 3009 3101 Size: 32 135 32 521 32 852 32 92 Cuts at: 3101 3133 3617 3649 3662 3694 3847 3879 4402 Size: 32 484 32 13 32 153 32 523 Cuts at: 4402 4434 4550 4582 7207 7239 7281 7313 1373 Size: 32 116 32 2625 32 42 32 2626 Fragments arranged by size: 2626 2625 852 523 521 484 153 135 116 92 42 32 32 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 3829 5910 6156 669 Size: 988 281 1891 2081 246 3079 Fragments arranged by size: 3079 2081 1891 988 281 246 HindIII A'AGCT_T Cuts at: 748 5024 748 Size: 4276 4290 HpaI GTT'AAC Cuts at: 6156 6156 Size: 8566 KasI G'GCGC_C Cuts at: 2035 2035 Size: 8566 KpnI G_GTAC'C Cuts at: 5891 5891 Size: 8566 MfeI C'AATT_G Cuts at: 3918 4243 6165 3918 Size: 325 1922 6319 Fragments arranged by size: 6319 1922 325 MluI A'CGCG_T Cuts at: 8442 8442 Size: 8566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2642 3441 3637 4236 7680 7864 1305 Size: 1337 799 196 599 3444 184 2007 Fragments arranged by size: 3444 2007 1337 799 599 196 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5046 10 Size: 54 1425 343 3214 3530 Fragments arranged by size: 3530 3214 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 2639 3024 3099 3288 3581 3783 Size: 648 1389 84 385 75 189 293 202 Cuts at: 3783 4361 5745 6524 6883 7042 518 Size: 578 1384 779 359 159 2042 Fragments arranged by size: 2042 1389 1384 779 648 578 385 359 293 202 189 159 84 75 MspA1I CmG'CkG Cuts at: 1606 5248 5982 6659 7807 8052 1606 Size: 3642 734 677 1148 245 2120 Fragments arranged by size: 3642 2120 1148 734 677 245 NarI GG'CG_CC Cuts at: 2036 2036 Size: 8566 NcoI C'CATG_G Cuts at: 513 1065 4677 4928 513 Size: 552 3612 251 4151 Fragments arranged by size: 4151 3612 552 251 NdeI CA'TA_TG Cuts at: 387 387 Size: 8566 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8566 NotI GC'GGCC_GC Cuts at: 6013 6013 Size: 8566 NspI r_CATG'y Cuts at: 1132 1229 2473 2501 2661 3023 5746 5924 7469 Size: 97 1244 28 160 362 2723 178 1545 Cuts at: 7469 1132 Size: 2229 Fragments arranged by size: 2723 2229 1545 1244 362 178 160 97 28 PasI CC'CwG_GG Cuts at: 1348 2340 1348 Size: 992 7574 PciI A'CATG_T Cuts at: 5742 7465 5742 Size: 1723 6843 PflMI CCAn_nnn'nTGG Cuts at: 1108 2771 4662 4929 5017 1108 Size: 1663 1891 267 88 4657 Fragments arranged by size: 4657 1891 1663 267 88 PfoI T'CCnGG_A Cuts at: 3228 3552 3729 4164 4401 5328 5511 3228 Size: 324 177 435 237 927 183 6283 Fragments arranged by size: 6283 927 435 324 237 183 177 PmeI GTTT'AAAC Cuts at: 5870 5870 Size: 8566 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6635 6667 8148 8180 6635 Size: 32 1481 32 7021 Fragments arranged by size: 7021 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2233 2233 Size: 8566 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8566 PsiI TTA'TAA Cuts at: 6136 6136 Size: 8566 PstI C_TGCA'G Cuts at: 830 2447 2571 2676 4026 5253 5808 5918 830 Size: 1617 124 105 1350 1227 555 110 3478 Fragments arranged by size: 3478 1617 1350 1227 555 124 110 105 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8566 SacI G_AGCT'C Cuts at: 4734 4734 Size: 8566 SalI G'TCGA_C Cuts at: 1936 5908 1936 Size: 3972 4594 SapI GCTCTTCn'nnn_ Cuts at: 5489 5489 Size: 8566 SbfI CC_TGCA'GG Cuts at: 5918 5918 Size: 8566 ScaI AGT'ACT Cuts at: 6814 8526 6814 Size: 1712 6854 SfcI C'TryA_G Cuts at: 826 1045 2443 2567 2672 4022 5249 5804 5914 Size: 219 1398 124 105 1350 1227 555 110 Cuts at: 5914 7049 7730 7921 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1398 1350 1227 1135 681 555 219 191 124 110 105 SfoI GGC'GCC Cuts at: 2037 2037 Size: 8566 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8566 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8566 SmaI CCC'GGG Cuts at: 1872 5893 1872 Size: 4021 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 3135 3392 3721 6670 7571 Size: 197 926 780 412 257 329 2949 901 Cuts at: 7571 7833 820 Size: 262 1553 Fragments arranged by size: 2949 1553 926 901 780 412 329 262 257 197 SnaBI TAC'GTA Cuts at: 493 6357 493 Size: 5864 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8566 SphI G_CATG'C Cuts at: 1132 5924 1132 Size: 4792 3774 SspI AAT'ATT Cuts at: 5 52 6490 5 Size: 47 6438 2081 Fragments arranged by size: 6438 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8566 StyI C'CwwG_G Cuts at: 513 1065 3426 4677 4928 5442 6005 513 Size: 552 2361 1251 251 514 563 3074 Fragments arranged by size: 3074 2361 1251 563 552 514 251 SwaI ATTT'AAAT Cuts at: 5470 5470 Size: 8566 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2653 3975 4654 4943 5308 6593 6610 Size: 1255 1073 1322 679 289 365 1285 17 Cuts at: 6610 6763 6948 7393 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1322 1285 1255 1073 679 445 365 289 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 3802 4033 Size: 274 80 33 51 1443 147 1678 231 Cuts at: 4033 4158 6812 8524 96 Size: 125 2654 1712 138 Fragments arranged by size: 2654 1712 1678 1443 274 231 147 138 125 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3849 3881 4416 4448 8526 8558 3849 Size: 32 535 32 4078 32 3857 Fragments arranged by size: 4078 3857 535 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8566 XbaI T'CTAG_A Cuts at: 5902 5902 Size: 8566 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4297 5054 5077 4297 Size: 757 23 7786 Fragments arranged by size: 7786 757 23 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8566 XmaI C'CCGG_G Cuts at: 1870 5891 1870 Size: 4021 4545 XmnI GAAnn'nnTTC Cuts at: 5369 6695 5369 Size: 1326 7240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5841 6374 276 Size: 53 83 186 936 4307 533 2468 Fragments arranged by size: 4307 2468 936 533 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI