(Circular) (Six-base) MAPSORT of: pFN21AA1065.seq Check: 3469 from: 1 to: 6628 pFN21AA1065 6628 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4438 278 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3877 3971 1937 Size: 1940 94 4594 Fragments arranged by size: 4594 1940 94 Acc65I G'GTAC_C Cuts at: 3152 3949 3152 Size: 797 5831 AclI AA'CG_TT Cuts at: 1563 4755 5128 1563 Size: 3192 373 3063 Fragments arranged by size: 3192 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2822 3114 3328 4691 6075 6343 1632 Size: 710 480 292 214 1363 1384 268 1917 Fragments arranged by size: 1917 1384 1363 710 480 292 268 214 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6431 AflIII A'CryG_T Cuts at: 1203 2275 3285 5527 6504 1203 Size: 1072 1010 2242 977 1327 Fragments arranged by size: 2242 1327 1072 1010 977 AgeI A'CCGG_T Cuts at: 5459 5459 Size: 6628 AhdI GACnn_n'nnGTC Cuts at: 5357 5357 Size: 6628 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6628 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2387 1184 Size: 1203 5425 AlwNI CAG_nnn'CTG Cuts at: 2320 2671 3688 4371 5943 2320 Size: 351 1017 683 1572 3005 Fragments arranged by size: 3005 1572 1017 683 351 ApaI G_GGCC'C Cuts at: 2108 2440 2641 3041 3071 3548 3845 2108 Size: 332 201 400 30 477 297 4891 Fragments arranged by size: 4891 477 400 332 297 201 30 ApaLI G'TGCA_C Cuts at: 4685 5841 4685 Size: 1156 5472 ApoI r'AATT_y Cuts at: 1493 2958 3383 3937 4163 4315 6424 1493 Size: 1465 425 554 226 152 2109 1697 Fragments arranged by size: 2109 1697 1465 554 425 226 152 AseI AT'TA_AT Cuts at: 160 1032 5182 160 Size: 872 4150 1606 Fragments arranged by size: 4150 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 AvaI C'yCGr_G Cuts at: 1870 1943 2766 3034 3953 1870 Size: 73 823 268 919 4545 Fragments arranged by size: 4545 919 823 268 73 BamHI G'GATC_C Cuts at: 3958 4325 3958 Size: 367 6261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3152 3187 3625 3949 5404 Size: 325 217 627 1365 35 438 324 1455 Cuts at: 5404 618 Size: 1842 Fragments arranged by size: 1842 1455 1365 627 438 325 324 217 35 BanII G_rGCy'C Cuts at: 1391 2108 2440 2579 2641 3041 3063 3071 3342 Size: 717 332 139 62 400 22 8 271 Cuts at: 3342 3548 3716 3845 3947 1391 Size: 206 168 129 102 4072 Fragments arranged by size: 4072 717 400 332 271 206 168 139 129 102 62 22 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5657 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4819 4853 1903 Size: 34 2882 34 3678 Fragments arranged by size: 3678 2882 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4520 5730 4520 Size: 1210 5418 BclI T'GATC_A Cuts at: 1548 1548 Size: 6628 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2724 3647 5239 Size: 107 122 71 631 605 1052 923 1592 Cuts at: 5239 136 Size: 1525 Fragments arranged by size: 1592 1525 1052 923 631 605 122 107 71 BglII A'GATC_T Cuts at: 6623 6623 Size: 6628 BlpI GC'TnA_GC Cuts at: 2877 4045 2877 Size: 1168 5460 Bme1580I G_kGCm'C Cuts at: 1790 2108 2440 2621 2641 3041 3071 3192 3520 Size: 318 332 181 20 400 30 121 328 Cuts at: 3520 3548 3845 4689 5845 1790 Size: 28 297 844 1156 2573 Fragments arranged by size: 2573 1156 844 400 332 328 318 297 181 121 30 28 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6628 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3598 3710 3848 Size: 416 53 83 399 24 2181 112 138 Cuts at: 3848 4359 5317 442 Size: 511 958 1753 Fragments arranged by size: 2181 1753 958 511 416 399 138 112 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6628 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2998 2998 Size: 6628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2503 2819 3216 3244 5288 1239 Size: 1264 316 397 28 2044 2579 Fragments arranged by size: 2579 2044 1264 397 316 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2390 3484 4753 5618 5916 2390 Size: 1094 1269 865 298 3102 Fragments arranged by size: 3102 1269 1094 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5291 882 Size: 617 3792 2219 Fragments arranged by size: 3792 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4419 493 Size: 711 3215 2702 Fragments arranged by size: 3215 2702 711 BsaBI GATnn'nnATC Cuts at: 6622 6622 Size: 6628 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4435 4817 5452 275 Size: 53 83 186 936 2902 382 635 1451 Fragments arranged by size: 2902 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5061 5459 5733 5880 6291 5061 Size: 398 274 147 411 5398 Fragments arranged by size: 5398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 1178 Size: 30 165 30 1140 30 5233 Fragments arranged by size: 5233 1140 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 2672 2846 1186 Size: 1207 279 174 4968 Fragments arranged by size: 4968 1207 279 174 BseYI C'CCAG_C Cuts at: 2283 2622 2644 2736 3029 3266 3509 3681 5831 Size: 339 22 92 293 237 243 172 2150 Cuts at: 5831 6327 2283 Size: 496 2584 Fragments arranged by size: 2584 2150 496 339 293 243 237 172 92 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6055 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4078 4839 5867 1540 Size: 275 186 2077 761 1028 2301 Fragments arranged by size: 2301 2077 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3342 3637 4689 4774 5845 3342 Size: 295 1052 85 1071 4125 Fragments arranged by size: 4125 1071 1052 295 85 BsmI GAATG_Cn' Cuts at: 4139 4232 4139 Size: 93 6535 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2440 2579 2621 2641 3041 3063 Size: 399 318 332 139 42 20 400 22 Cuts at: 3063 3071 3192 3342 3520 3548 3637 3716 3845 Size: 8 121 150 178 28 89 79 129 Cuts at: 3845 3947 4689 4774 5845 1391 Size: 102 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 400 399 332 318 178 150 139 129 121 102 89 85 79 42 28 22 20 8 BspEI T'CCGG_A Cuts at: 6291 6291 Size: 6628 BspHI T'CATG_A Cuts at: 2172 4517 6247 2172 Size: 2345 1730 2553 Fragments arranged by size: 2553 2345 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3983 844 Size: 3139 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4515 1394 Size: 429 2692 3507 Fragments arranged by size: 3507 2692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3855 5123 5297 65 Size: 1183 2607 1268 174 1396 Fragments arranged by size: 2607 1396 1268 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5272 5459 1700 Size: 110 3462 187 2869 Fragments arranged by size: 3462 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2226 96 Size: 2130 4498 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6628 BssSI C'ACGA_G Cuts at: 4688 5700 4688 Size: 1012 5616 BstAPI GCAn_nnn'nTGC Cuts at: 794 3632 794 Size: 2838 3790 BstBI TT'CG_AA Cuts at: 5432 5432 Size: 6628 BstEII G'GTnAC_C Cuts at: 1184 3580 1184 Size: 2396 4232 BstXI CCAn_nnnn'nTGG Cuts at: 2485 2822 3449 3772 3902 2485 Size: 337 627 323 130 5211 Fragments arranged by size: 5211 627 337 323 130 BstYI r'GATC_y Cuts at: 1972 3958 4325 4710 4727 6179 6265 6277 6623 Size: 1986 367 385 17 1452 86 12 346 Cuts at: 6623 1972 Size: 1977 Fragments arranged by size: 1986 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3878 3878 Size: 6628 Bsu36I CC'TnA_GG Cuts at: 2604 2894 2604 Size: 290 6338 BtgI C'CryG_G Cuts at: 513 1065 2099 2203 2593 3042 3179 3765 3789 Size: 552 1034 104 390 449 137 586 24 Cuts at: 3789 513 Size: 3352 Fragments arranged by size: 3352 1034 586 552 449 390 137 104 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5889 Fragments arranged by size: 5889 683 56 BtsI GCAGTG_nn' Cuts at: 2294 3520 3874 4144 4938 4958 2294 Size: 1226 354 270 794 20 3964 Fragments arranged by size: 3964 1226 794 354 270 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6628 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6593 DraI TTT'AAA Cuts at: 3932 3932 Size: 6628 DraIII CAC_nnn'GTG Cuts at: 3632 3632 Size: 6628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2014 5635 809 Size: 507 698 3621 1802 Fragments arranged by size: 3621 1802 698 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3140 4075 4964 Size: 54 1425 50 275 18 1310 935 889 Cuts at: 4964 6593 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1310 935 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4075 1537 Size: 275 2263 4090 Fragments arranged by size: 4090 2263 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2823 3347 4558 1353 Size: 1470 524 1211 3423 Fragments arranged by size: 3423 1470 1211 524 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5215 5589 5735 387 Size: 1064 138 3626 374 146 1280 Fragments arranged by size: 3626 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3340 3340 Size: 6628 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2503 2819 2822 3114 3216 3244 Size: 393 710 161 316 3 292 102 28 Cuts at: 3244 3328 4691 5288 6075 6343 1239 Size: 84 1363 597 787 268 1524 Fragments arranged by size: 1524 1363 787 710 597 393 316 292 268 161 102 84 28 3 EcoNI CCTnn'n_nnAGG Cuts at: 3750 3750 Size: 6628 EcoO109I rG'GnC_Cy Cuts at: 2104 2436 2637 2638 3038 3067 3330 3545 3842 Size: 332 201 1 400 29 263 215 297 Cuts at: 3842 2104 Size: 4890 Fragments arranged by size: 4890 400 332 297 263 215 201 29 1 EcoRI G'AATT_C Cuts at: 3937 4315 3937 Size: 378 6250 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2852 2852 Size: 6628 FspI TGC'GCA Cuts at: 5134 5134 Size: 6628 HaeII r_GCGC'y Cuts at: 1678 2237 2683 5775 1678 Size: 559 446 3092 2531 Fragments arranged by size: 3092 2531 559 446 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5269 5301 5343 5375 1373 Size: 32 135 32 3697 32 42 32 2626 Fragments arranged by size: 3697 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3658 3972 4218 669 Size: 988 281 1720 314 246 3079 Fragments arranged by size: 3079 1720 988 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6628 HpaI GTT'AAC Cuts at: 3658 4218 3658 Size: 560 6068 KpnI G_GTAC'C Cuts at: 3156 3953 3156 Size: 797 5831 MfeI C'AATT_G Cuts at: 4227 4227 Size: 6628 MluI A'CGCG_T Cuts at: 3285 6504 3285 Size: 3219 3409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2988 5742 5926 1305 Size: 1683 2754 184 2007 Fragments arranged by size: 2754 2007 1683 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3142 10 Size: 54 1425 343 1310 3496 Fragments arranged by size: 3496 1425 1310 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2369 2483 3770 4586 4945 5104 Size: 648 1011 192 114 1287 816 359 159 Cuts at: 5104 518 Size: 2042 Fragments arranged by size: 2042 1287 1011 816 648 359 192 159 114 MspA1I CmG'CkG Cuts at: 1606 2644 2842 2848 3044 3113 3164 3688 3791 Size: 1038 198 6 196 69 51 524 103 Cuts at: 3791 4044 4721 5869 6114 1606 Size: 253 677 1148 245 2120 Fragments arranged by size: 2120 1148 1038 677 524 253 245 198 196 103 69 51 6 NcoI C'CATG_G Cuts at: 513 1065 2203 2593 3179 3765 513 Size: 552 1138 390 586 586 3376 Fragments arranged by size: 3376 1138 586 586 552 390 NdeI CA'TA_TG Cuts at: 387 387 Size: 6628 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6628 NotI GC'GGCC_GC Cuts at: 4075 4075 Size: 6628 NspI r_CATG'y Cuts at: 1132 1229 2225 3986 5531 1132 Size: 97 996 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 996 97 PasI CC'CwG_GG Cuts at: 1348 3063 3099 3234 1348 Size: 1715 36 135 4742 Fragments arranged by size: 4742 1715 135 36 PciI A'CATG_T Cuts at: 5527 5527 Size: 6628 PflMI CCAn_nnn'nTGG Cuts at: 1108 3099 3149 1108 Size: 1991 50 4587 Fragments arranged by size: 4587 1991 50 PfoI T'CCnGG_A Cuts at: 2076 2076 Size: 6628 PmeI GTTT'AAAC Cuts at: 3932 3932 Size: 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2039 2071 3557 3589 4697 4729 6210 6242 2039 Size: 32 1486 32 1108 32 1481 32 2425 Fragments arranged by size: 2425 1486 1481 1108 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3330 3330 Size: 6628 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6628 PsiI TTA'TAA Cuts at: 4198 4198 Size: 6628 PspOMI G'GGCC_C Cuts at: 2104 2436 2637 3037 3067 3544 3841 2104 Size: 332 201 400 30 477 297 4891 Fragments arranged by size: 4891 477 400 332 297 201 30 PstI C_TGCA'G Cuts at: 830 2667 2847 3980 830 Size: 1837 180 1133 3478 Fragments arranged by size: 3478 1837 1133 180 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6628 PvuII CAG'CTG Cuts at: 2842 2848 3164 2842 Size: 6 316 6306 Fragments arranged by size: 6306 316 6 SacI G_AGCT'C Cuts at: 3342 3342 Size: 6628 SacII CC_GC'GG Cuts at: 3045 3792 3045 Size: 747 5881 SalI G'TCGA_C Cuts at: 1936 3970 1936 Size: 2034 4594 SapI GCTCTTCn'nnn_ Cuts at: 3347 3347 Size: 6628 SbfI CC_TGCA'GG Cuts at: 3980 3980 Size: 6628 ScaI AGT'ACT Cuts at: 3127 4876 6588 3127 Size: 1749 1712 3167 Fragments arranged by size: 3167 1749 1712 SexAI A'CCwGG_T Cuts at: 3628 3628 Size: 6628 SfcI C'TryA_G Cuts at: 826 1045 2663 2843 3976 5111 5792 5983 826 Size: 219 1618 180 1133 1135 681 191 1471 Fragments arranged by size: 1618 1471 1135 1133 681 219 191 180 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6628 SmaI CCC'GGG Cuts at: 1872 3955 1872 Size: 2083 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 3499 4732 5633 5895 820 Size: 197 926 462 1094 1233 901 262 1553 Fragments arranged by size: 1553 1233 1094 926 901 462 262 197 SnaBI TAC'GTA Cuts at: 493 4419 493 Size: 3926 2702 SpeI A'CTAG_T Cuts at: 152 2819 152 Size: 2667 3961 SphI G_CATG'C Cuts at: 1132 3986 1132 Size: 2854 3774 SspI AAT'ATT Cuts at: 5 52 4552 5 Size: 47 4500 2081 Fragments arranged by size: 4500 2081 47 StuI AGG'CCT Cuts at: 1517 2609 3589 1517 Size: 1092 980 4556 Fragments arranged by size: 4556 1092 980 StyI C'CwwG_G Cuts at: 513 1065 2203 2593 3179 3765 4067 513 Size: 552 1138 390 586 586 302 3074 Fragments arranged by size: 3074 1138 586 586 552 390 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2141 2358 4655 4672 4825 5010 5455 Size: 1255 561 217 2297 17 153 185 445 Cuts at: 5455 325 Size: 1498 Fragments arranged by size: 2297 1498 1255 561 445 217 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2334 3125 Size: 274 80 33 51 1443 249 108 791 Cuts at: 3125 4874 6586 96 Size: 1749 1712 138 Fragments arranged by size: 1749 1712 1443 791 274 249 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3198 3230 6588 6620 3198 Size: 32 3358 32 3206 Fragments arranged by size: 3358 3206 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2014 1316 Size: 698 5930 XbaI T'CTAG_A Cuts at: 3964 3964 Size: 6628 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2461 2477 2504 2727 3556 2461 Size: 16 27 223 829 5533 Fragments arranged by size: 5533 829 223 27 16 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6628 XmaI C'CCGG_G Cuts at: 1870 3953 1870 Size: 2083 4545 XmnI GAAnn'nnTTC Cuts at: 2303 2327 4757 2303 Size: 24 2430 4174 Fragments arranged by size: 4174 2430 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4436 276 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI