Restriction map for
pFN21AA1041
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BcgI BcnI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BseAI
BsgI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT14I FauI FseI
FspI HaeII HgaI Hin1I HindIII
KasI KpnI KspI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PleI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1432 GTAGAC GT^[AC][GT]AC
AgeI 756 ACCGGT A^CCGGT
AseI 271 ATTAAT AT^TAAT
AsnI 271 ATTAAT AT^TAAT
AspHI 1014 GAGCAC G[AT]GC[AT]^C
BbvCI 799 CCTCAGC CCTCAGC(-5/-2)
BciVI 1336 GTATCC GTATCC(6/5)
BclI 648 TGATCA T^GATCA
BfaI 1664 CTAG C^TAG
BglI 489 GCCTACTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 939 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1101 GGTCTC GGTCTC(1/5)
BsaWI 756 ACCGGT [AT]^CCGG[AT]
BseRI 797 CTCCTC GAGGAG(10/8)
BsiEI 1156 CGACCG CG[GA][CT]^CG
BsiHKAI 1014 GAGCAC G[AT]GC[AT]^C
BsmI 168 GAATGC GAATGC(1/-1)
Bsp1407I 1652 TGTACA T^GTACA
BsrFI 756 ACCGGT [GA]^CCGG[CT]
BsrGI 1652 TGTACA T^GTACA
BstAPI 1779 GCACATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 86 GGTTACC G^GT[ACGT]ACC
BstPI 86 GGTTACC G^GT[ACGT]ACC
BstXI 1629 CCAACACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 484 GCGC GCG^C
Cfr10I 756 ACCGGT [GA]^CCGG[CT]
DraII 445 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 14 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 496 CGGCCA [CT]^GGCC[GA]
EarI 504 GAAGAG CTCTTC(1/4)
EcoO109I 445 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 86 GGTTACC G^GT[ACGT]ACC
EcoRI 369 GAATTC G^AATTC
EcoT22I 1609 ATGCAT ATGCA^T
FbaI 648 TGATCA T^GATCA
HapII 757 CCGG C^CGG
HhaI 484 GCGC GCG^C
HinP1I 484 GCGC G^CGC
HpaI 1302 GTTAAC GTT^AAC
HpaII 757 CCGG C^CGG
Ksp632I 504 GAAGAG CTCTTC(1/4)
MaeI 1664 CTAG C^TAG
MamI 939 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1859 CAATTG C^AATTG
MspI 757 CCGG C^CGG
MunI 1859 CAATTG C^AATTG
NsiI 1609 ATGCAT ATGCA^T
PinAI 756 ACCGGT A^CCGGT
PpuMI 445 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 271 ATTAAT AT^TAAT
SexAI 1502 ACCAGGT A^CC[AT]GGT
SfcI 518 CTGTAG C^T[GA][CT]AG
SgrAI 755 CACCGGTG C[GA]^CCGG[CT]G
SmlI 1483 CTCAAG C^T[CT][GA]AG
SspBI 1652 TGTACA T^GTACA
XcmI 1152 CCATCGACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 198 GGACC G^G[AT]CC
446 GGTCC
BanI 1314 GGCACC G^G[CT][GA]CC
1603 GGTGCC
BglII 870 AGATCT A^GATCT
1479 AGATCT
BmyI 509 GGGCAC G[AGT]GC[ACT]^C
1014 GAGCAC
BsaJI 435 CCCTGG C^C[ACGT][ACGT]GG
1698 CCCTGG
BsmFI 969 GTCCC GGGAC(10/14)
1233 GTCCC
Bsp1286I 509 GGGCAC G[AGT]GC[ACT]^C
1014 GAGCAC
BstYI 870 AGATCT [GA]^GATC[CT]
1479 AGATCT
HincII 926 GTCAAC GT[CT]^[GA]AC
1302 GTTAAC
HindII 926 GTCAAC GT[CT]^[GA]AC
1302 GTTAAC
MaeII 73 ACGT A^CGT
1300 ACGT
MflI 870 AGATCT [GA]^GATC[CT]
1479 AGATCT
MslI 220 CACAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1057 CATGGCAGTG
PflMI 702 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1693 CCACCCCCTGG
Van91I 702 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1693 CCACCCCCTGG
XhoII 870 AGATCT [GA]^GATC[CT]
1479 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BpmI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I