Restriction map for
pFN21AA1041
(Linear) (Six-base) MAPSORT of: KIAA1041.seq Check: 2436 from: 1 to: 1866
KIAA1041.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AccI GT'mk_AC
Cuts at: 0 1433 1866
Size: 1433 433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1667 1866
Size: 1667 199
AgeI A'CCGG_T
Cuts at: 0 756 1866
Size: 756 1110
ApoI r'AATT_y
Cuts at: 0 155 308 369 1647 1866
Size: 155 153 61 1278 219
Fragments arranged by size:
1278 219 155 153 61
AseI AT'TA_AT
Cuts at: 0 272 1866
Size: 272 1594
BanI G'GyrC_C
Cuts at: 0 1314 1603 1866
Size: 1314 289 263
Fragments arranged by size:
1314 289 263
BbvCI CC'TCA_GC
Cuts at: 0 800 1866
Size: 800 1066
BciVI GTATCCnnnnn_n'
Cuts at: 0 1347 1866
Size: 1347 519
BclI T'GATC_A
Cuts at: 0 648 1866
Size: 648 1218
BfrBI ATG'CAT
Cuts at: 0 1611 1866
Size: 1611 255
BglI GCCn_nnn'nGGC
Cuts at: 0 495 1866
Size: 495 1371
BglII A'GATC_T
Cuts at: 0 870 1479 1866
Size: 870 609 387
Fragments arranged by size:
870 609 387
Bme1580I G_kGCm'C
Cuts at: 0 513 1866
Size: 513 1353
BmrI ACTGGGnnnn_n'
Cuts at: 0 462 1866
Size: 462 1404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1213 1718 1808 1866
Size: 79 1134 505 90 58
Fragments arranged by size:
1134 505 90 79 58
Bpu10I CC'TnA_GC
Cuts at: 0 800 1866
Size: 800 1066
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1468 1866
Size: 1468 398
BsaI GGTCTCn'nnnn_
Cuts at: 0 1107 1866
Size: 1107 759
BsaBI GATnn'nnATC
Cuts at: 0 943 1866
Size: 943 923
BsaWI w'CCGG_w
Cuts at: 0 756 1866
Size: 756 1110
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 788 1866
Size: 788 1078
BseYI C'CCAG_C
Cuts at: 0 962 1000 1197 1244 1866
Size: 962 38 197 47 622
Fragments arranged by size:
962 622 197 47 38
BsiEI CG_ry'CG
Cuts at: 0 1159 1866
Size: 1159 707
BsiHKAI G_wGCw'C
Cuts at: 0 1018 1866
Size: 1018 848
BsmI GAATG_Cn'
Cuts at: 0 174 1866
Size: 174 1692
Bsp1286I G_dGCh'C
Cuts at: 0 513 1018 1866
Size: 513 505 848
Fragments arranged by size:
848 513 505
BsrFI r'CCGG_y
Cuts at: 0 756 1866
Size: 756 1110
BsrGI T'GTAC_A
Cuts at: 0 1652 1866
Size: 1652 214
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1785 1866
Size: 1785 81
BstEII G'GTnAC_C
Cuts at: 0 86 1866
Size: 86 1780
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1636 1866
Size: 1636 230
BstYI r'GATC_y
Cuts at: 0 870 1479 1866
Size: 870 609 387
Fragments arranged by size:
870 609 387
BtsI GCAGTG_nn'
Cuts at: 0 363 1068 1419 1866
Size: 363 705 351 447
Fragments arranged by size:
705 447 363 351
DrdI GACnn_nn'nnGTC
Cuts at: 0 20 1866
Size: 20 1846
EaeI y'GGCC_r
Cuts at: 0 496 1866
Size: 496 1370
EarI CTCTTCn'nnn_
Cuts at: 0 499 1866
Size: 499 1367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1213 1667 1718 1808 1866
Size: 79 1134 454 51 90 58
Fragments arranged by size:
1134 454 90 79 58 51
EcoO109I rG'GnC_Cy
Cuts at: 0 446 1866
Size: 446 1420
EcoRI G'AATT_C
Cuts at: 0 369 1866
Size: 369 1497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 753 785 1866
Size: 98 32 623 32 1081
Fragments arranged by size:
1081 623 98 32 32
HincII GTy'rAC
Cuts at: 0 928 1304 1866
Size: 928 376 562
Fragments arranged by size:
928 562 376
HpaI GTT'AAC
Cuts at: 0 1304 1866
Size: 1304 562
MfeI C'AATT_G
Cuts at: 0 1859 1866
Size: 1859 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 343 622 714 1073 1639 1866
Size: 237 106 279 92 359 566 227
Fragments arranged by size:
566 359 279 237 227 106 92
MslI CAynn'nnrTG
Cuts at: 0 224 1061 1866
Size: 224 837 805
Fragments arranged by size:
837 805 224
NsiI A_TGCA'T
Cuts at: 0 1613 1866
Size: 1613 253
PflMI CCAn_nnn'nTGG
Cuts at: 0 708 1699 1866
Size: 708 991 167
Fragments arranged by size:
991 708 167
PfoI T'CCnGG_A
Cuts at: 0 1734 1835 1866
Size: 1734 101 31
Fragments arranged by size:
1734 101 31
PpuMI rG'GwC_Cy
Cuts at: 0 446 1866
Size: 446 1420
PsiI TTA'TAA
Cuts at: 0 899 1866
Size: 899 967
SexAI A'CCwGG_T
Cuts at: 0 1502 1866
Size: 1502 364
SfcI C'TryA_G
Cuts at: 0 518 1866
Size: 518 1348
SgrAI Cr'CCGG_yG
Cuts at: 0 756 1866
Size: 756 1110
SmlI C'TyrA_G
Cuts at: 0 1483 1866
Size: 1483 383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 297 1037 1142 1211 1223 1866
Size: 297 740 105 69 12 643
Fragments arranged by size:
740 643 297 105 69 12
TatI w'GTAC_w
Cuts at: 0 815 1006 1652 1866
Size: 815 191 646 214
Fragments arranged by size:
815 646 214 191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1159 1866
Size: 1159 707
Enzymes that do cut:
AccI AcuI AgeI ApoI AseI BanI BbvCI BciVI
BclI BfrBI BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BsrFI BsrGI BstAPI BstEII BstXI BstYI
BtsI DrdI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I
HincII HpaI MfeI MmeI MslI NsiI PflMI PfoI
PpuMI PsiI SexAI SfcI SgrAI SmlI TaqII TatI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbsI BcgI
BlpI BmgBI BmtI BplI BsaAI BsaHI BsaXI BsgI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII
BssSI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HindIII KasI KpnI MluI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI