(Circular) (Six-base) MAPSORT of: pFN21AA1017.seq Check: 1161 from: 1 to: 7750 pFN21AA1017 7750 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5560 278 Size: 53 83 186 936 4024 2468 Fragments arranged by size: 4024 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5093 1937 Size: 3156 4594 Acc65I G'GTAC_C Cuts at: 5071 5071 Size: 7750 AclI AA'CG_TT Cuts at: 1563 5877 6250 1563 Size: 4314 373 3063 Fragments arranged by size: 4314 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2001 3263 4016 4389 5813 7197 7465 1632 Size: 369 1262 753 373 1424 1384 268 1917 Fragments arranged by size: 1917 1424 1384 1262 753 373 369 268 AfeI AGC'GCT Cuts at: 2738 2738 Size: 7750 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7553 AflIII A'CryG_T Cuts at: 1203 3940 6649 7626 1203 Size: 2737 2709 977 1327 Fragments arranged by size: 2737 2709 1327 977 AgeI A'CCGG_T Cuts at: 6581 6581 Size: 7750 AhdI GACnn_n'nnGTC Cuts at: 6479 6479 Size: 7750 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7750 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7750 AlwNI CAG_nnn'CTG Cuts at: 2147 3647 3994 4956 5493 7065 2147 Size: 1500 347 962 537 1572 2832 Fragments arranged by size: 2832 1572 1500 962 537 347 ApaI G_GGCC'C Cuts at: 4886 4886 Size: 7750 ApaLI G'TGCA_C Cuts at: 5807 6963 5807 Size: 1156 6594 ApoI r'AATT_y Cuts at: 1493 2592 2806 2930 3110 3913 3975 5059 5285 Size: 1099 214 124 180 803 62 1084 226 Cuts at: 5285 5437 7546 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1099 1084 803 226 214 180 152 124 62 AseI AT'TA_AT Cuts at: 160 1032 6304 160 Size: 872 5272 1606 Fragments arranged by size: 5272 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7750 AvaI C'yCGr_G Cuts at: 1870 1943 5075 1870 Size: 73 3132 4545 Fragments arranged by size: 4545 3132 73 BamHI G'GATC_C Cuts at: 2516 4725 5080 5447 2516 Size: 2209 355 367 4819 Fragments arranged by size: 4819 2209 367 355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2885 3335 5071 6526 618 Size: 325 217 627 1098 450 1736 1455 1842 Fragments arranged by size: 1842 1736 1455 1098 627 450 325 217 BanII G_rGCy'C Cuts at: 1391 3353 3378 4886 5069 1391 Size: 1962 25 1508 183 4072 Fragments arranged by size: 4072 1962 1508 183 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2007 3063 3177 3444 3489 3851 928 Size: 971 108 1056 114 267 45 362 4827 Fragments arranged by size: 4827 1056 971 362 267 114 108 45 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5941 5975 1903 Size: 34 4004 34 3678 Fragments arranged by size: 4004 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2050 4072 5642 6852 2050 Size: 2022 1570 1210 2948 Fragments arranged by size: 2948 2022 1570 1210 BclI T'GATC_A Cuts at: 1548 2086 2503 1548 Size: 538 417 6795 Fragments arranged by size: 6795 538 417 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6361 136 Size: 107 122 71 631 605 4689 1525 Fragments arranged by size: 4689 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4147 7745 4147 Size: 3598 4152 BlpI GC'TnA_GC Cuts at: 5167 5167 Size: 7750 Bme1580I G_kGCm'C Cuts at: 1790 4886 5811 6967 1790 Size: 3096 925 1156 2573 Fragments arranged by size: 3096 2573 1156 925 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7750 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2518 5481 6439 Size: 416 53 83 399 24 1101 2963 958 Cuts at: 6439 442 Size: 1753 Fragments arranged by size: 2963 1753 1101 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7750 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3174 3459 4670 6410 1239 Size: 1935 285 1211 1740 2579 Fragments arranged by size: 2579 1935 1740 1211 285 Bpu10I CC'TnA_GC Cuts at: 3377 4872 3377 Size: 1495 6255 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4412 4949 5875 6740 7038 4412 Size: 537 926 865 298 5124 Fragments arranged by size: 5124 926 865 537 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3361 4727 6413 882 Size: 617 1862 1366 1686 2219 Fragments arranged by size: 2219 1862 1686 1366 617 BsaAI yAC'GTr Cuts at: 493 1204 5541 493 Size: 711 4337 2702 Fragments arranged by size: 4337 2702 711 BsaBI GATnn'nnATC Cuts at: 7744 7744 Size: 7750 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5557 5939 6574 275 Size: 53 83 186 936 4024 382 635 1451 Fragments arranged by size: 4024 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6183 6581 6855 7002 7413 6183 Size: 398 274 147 411 6520 Fragments arranged by size: 6520 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3050 3080 4807 4837 1178 Size: 30 165 30 1647 30 1727 30 4091 Fragments arranged by size: 4091 1727 1647 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 3255 3461 1186 Size: 1255 814 206 5475 Fragments arranged by size: 5475 1255 814 206 BseYI C'CCAG_C Cuts at: 2052 2093 5040 6953 7449 2052 Size: 41 2947 1913 496 2353 Fragments arranged by size: 2947 2353 1913 496 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7177 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5026 5200 5961 6989 1540 Size: 275 186 3025 174 761 1028 2301 Fragments arranged by size: 3025 2301 1028 761 275 186 174 BsiHKAI G_wGCw'C Cuts at: 2380 3378 4784 5811 5896 6967 2380 Size: 998 1406 1027 85 1071 3163 Fragments arranged by size: 3163 1406 1071 1027 998 85 BsmI GAATG_Cn' Cuts at: 3237 5261 5354 3237 Size: 2024 93 5633 Fragments arranged by size: 5633 2024 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2380 3353 3378 4784 4886 5069 5811 Size: 399 590 973 25 1406 102 183 742 Cuts at: 5811 5896 6967 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1406 1071 973 742 590 399 183 102 85 25 BspEI T'CCGG_A Cuts at: 7413 7413 Size: 7750 BspHI T'CATG_A Cuts at: 5639 7369 5639 Size: 1730 6020 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3144 3431 4593 4964 5105 844 Size: 2300 287 1162 371 141 3489 Fragments arranged by size: 3489 2300 1162 371 287 141 BsrBI CCG'CTC Cuts at: 1394 1823 5637 1394 Size: 429 3814 3507 Fragments arranged by size: 3814 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2814 3528 3760 3846 6245 6419 65 Size: 1183 1566 714 232 86 2399 174 1396 Fragments arranged by size: 2399 1566 1396 1183 714 232 174 86 BsrFI r'CCGG_y Cuts at: 1700 1810 6394 6581 1700 Size: 110 4584 187 2869 Fragments arranged by size: 4584 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2200 96 Size: 2104 5646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7750 BssSI C'ACGA_G Cuts at: 3346 4783 5810 6822 3346 Size: 1437 1027 1012 4274 Fragments arranged by size: 4274 1437 1027 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2147 2569 794 Size: 1353 422 5975 Fragments arranged by size: 5975 1353 422 BstBI TT'CG_AA Cuts at: 6554 6554 Size: 7750 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7750 BstXI CCAn_nnnn'nTGG Cuts at: 4477 4846 4477 Size: 369 7381 BstYI r'GATC_y Cuts at: 1972 2516 4147 4725 5080 5447 5832 5849 7301 Size: 544 1631 578 355 367 385 17 1452 Cuts at: 7301 7387 7399 7745 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1631 1452 578 544 385 367 355 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4967 4967 Size: 7750 BtgI C'CryG_G Cuts at: 513 1065 2881 4737 4878 513 Size: 552 1816 1856 141 3385 Fragments arranged by size: 3385 1856 1816 552 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7011 Fragments arranged by size: 7011 683 56 BtsI GCAGTG_nn' Cuts at: 4449 4901 5266 6060 6080 4449 Size: 452 365 794 20 6119 Fragments arranged by size: 6119 794 452 365 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7750 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7715 DraI TTT'AAA Cuts at: 3106 5054 3106 Size: 1948 5802 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6757 809 Size: 507 5441 1802 Fragments arranged by size: 5441 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5197 6086 7715 Size: 54 1425 50 275 18 3367 889 1629 Cuts at: 7715 8 Size: 43 Fragments arranged by size: 3367 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5197 1537 Size: 275 3385 4090 Fragments arranged by size: 4090 3385 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2029 2755 3031 3123 3282 3396 4257 4654 Size: 676 726 276 92 159 114 861 397 Cuts at: 4654 5680 1353 Size: 1026 3423 Fragments arranged by size: 3423 1026 861 726 676 397 276 159 114 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6337 6711 6857 387 Size: 1064 138 4748 374 146 1280 Fragments arranged by size: 4748 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3376 3376 Size: 7750 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2001 3174 3263 3459 4016 4389 4670 Size: 393 369 1173 89 196 557 373 281 Cuts at: 4670 5813 6410 7197 7465 1239 Size: 1143 597 787 268 1524 Fragments arranged by size: 1524 1173 1143 787 597 557 393 373 369 281 268 196 89 EcoO109I rG'GnC_Cy Cuts at: 3133 3538 4992 3133 Size: 405 1454 5891 Fragments arranged by size: 5891 1454 405 EcoRI G'AATT_C Cuts at: 3110 3913 5059 5437 3110 Size: 803 1146 378 5423 Fragments arranged by size: 5423 1146 803 378 EcoRV GAT'ATC Cuts at: 2727 4297 4628 4963 2727 Size: 1570 331 335 5514 Fragments arranged by size: 5514 1570 335 331 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2645 2853 3762 3825 4021 2645 Size: 208 909 63 196 6374 Fragments arranged by size: 6374 909 208 196 63 FspI TGC'GCA Cuts at: 6256 6256 Size: 7750 HaeII r_GCGC'y Cuts at: 1678 2740 6897 1678 Size: 1062 4157 2531 Fragments arranged by size: 4157 2531 1062 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3044 3050 3076 3082 3340 Size: 32 135 32 1472 6 26 6 258 Cuts at: 3340 3360 3372 3377 3392 3409 3641 3673 4248 Size: 20 12 5 15 17 232 32 575 Cuts at: 4248 4280 4364 4396 4531 4563 6391 6423 6465 Size: 32 84 32 135 32 1828 32 42 Cuts at: 6465 6497 1373 Size: 32 2626 Fragments arranged by size: 2626 1828 1472 575 258 232 135 135 84 42 32 32 32 32 32 32 32 32 26 20 17 15 12 6 6 5 HincII GTy'rAC Cuts at: 669 1657 1938 2188 5094 5340 669 Size: 988 281 250 2906 246 3079 Fragments arranged by size: 3079 2906 988 281 250 246 HindIII A'AGCT_T Cuts at: 748 4046 4751 748 Size: 3298 705 3747 Fragments arranged by size: 3747 3298 705 HpaI GTT'AAC Cuts at: 5340 5340 Size: 7750 KpnI G_GTAC'C Cuts at: 5075 5075 Size: 7750 MfeI C'AATT_G Cuts at: 5349 5349 Size: 7750 MluI A'CGCG_T Cuts at: 7626 7626 Size: 7750 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4404 4703 6864 7048 1305 Size: 3099 299 2161 184 2007 Fragments arranged by size: 3099 2161 2007 299 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5928 Fragments arranged by size: 5928 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2556 2697 3165 3784 4844 5708 Size: 648 843 547 141 468 619 1060 864 Cuts at: 5708 6067 6226 518 Size: 359 159 2042 Fragments arranged by size: 2042 1060 864 843 648 619 547 468 359 159 141 MspA1I CmG'CkG Cuts at: 1606 2144 2513 2875 4378 4537 5166 5843 6991 Size: 538 369 362 1503 159 629 677 1148 Cuts at: 6991 7236 1606 Size: 245 2120 Fragments arranged by size: 2120 1503 1148 677 629 538 369 362 245 159 NcoI C'CATG_G Cuts at: 513 1065 2881 4878 513 Size: 552 1816 1997 3385 Fragments arranged by size: 3385 1997 1816 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7750 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7750 NotI GC'GGCC_GC Cuts at: 5197 5197 Size: 7750 NspI r_CATG'y Cuts at: 1132 1229 2793 3783 3944 5011 5108 6653 1132 Size: 97 1564 990 161 1067 97 1545 2229 Fragments arranged by size: 2229 1564 1545 1067 990 161 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7750 PciI A'CATG_T Cuts at: 3940 6649 3940 Size: 2709 5041 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7750 PfoI T'CCnGG_A Cuts at: 2335 3492 2335 Size: 1157 6593 PmeI GTTT'AAAC Cuts at: 5054 5054 Size: 7750 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5819 5851 7332 7364 5819 Size: 32 1481 32 6205 Fragments arranged by size: 6205 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3133 3538 3133 Size: 405 7345 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7750 PsiI TTA'TAA Cuts at: 2426 4574 5320 2426 Size: 2148 746 4856 Fragments arranged by size: 4856 2148 746 PspOMI G'GGCC_C Cuts at: 4882 4882 Size: 7750 PstI C_TGCA'G Cuts at: 830 2848 4567 4604 5102 830 Size: 2018 1719 37 498 3478 Fragments arranged by size: 3478 2018 1719 498 37 PvuI CG_AT'CG Cuts at: 2001 5026 2001 Size: 3025 4725 PvuII CAG'CTG Cuts at: 2144 2513 2875 4537 2144 Size: 369 362 1662 5357 Fragments arranged by size: 5357 1662 369 362 SacI G_AGCT'C Cuts at: 3378 3378 Size: 7750 SalI G'TCGA_C Cuts at: 1936 5092 1936 Size: 3156 4594 SapI GCTCTTCn'nnn_ Cuts at: 4257 4257 Size: 7750 SbfI CC_TGCA'GG Cuts at: 5102 5102 Size: 7750 ScaI AGT'ACT Cuts at: 3965 4004 5998 7710 3965 Size: 39 1994 1712 4005 Fragments arranged by size: 4005 1994 1712 39 SfcI C'TryA_G Cuts at: 826 1045 2668 2844 3533 4563 4600 4907 5098 Size: 219 1623 176 689 1030 37 307 191 Cuts at: 5098 6233 6914 7105 826 Size: 1135 681 191 1471 Fragments arranged by size: 1623 1471 1135 1030 689 681 307 219 191 191 176 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7750 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7750 SmaI CCC'GGG Cuts at: 1872 5077 1872 Size: 3205 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4391 4928 5854 6755 7017 820 Size: 197 926 2448 537 926 901 262 1553 Fragments arranged by size: 2448 1553 926 926 901 537 262 197 SnaBI TAC'GTA Cuts at: 493 5541 493 Size: 5048 2702 SpeI A'CTAG_T Cuts at: 152 3464 152 Size: 3312 4438 SphI G_CATG'C Cuts at: 1132 5011 5108 1132 Size: 3879 97 3774 Fragments arranged by size: 3879 3774 97 SspI AAT'ATT Cuts at: 5 52 3711 4678 5674 5 Size: 47 3659 967 996 2081 Fragments arranged by size: 3659 2081 996 967 47 StuI AGG'CCT Cuts at: 1517 2766 4366 4501 4667 1517 Size: 1249 1600 135 166 4600 Fragments arranged by size: 4600 1600 1249 166 135 StyI C'CwwG_G Cuts at: 513 1065 2881 3800 4878 5189 513 Size: 552 1816 919 1078 311 3074 Fragments arranged by size: 3074 1816 1078 919 552 311 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5777 5794 5947 6132 6577 325 Size: 1255 4197 17 153 185 445 1498 Fragments arranged by size: 4197 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2200 2429 3963 Size: 274 80 33 51 1443 223 229 1534 Cuts at: 3963 4002 5996 7708 96 Size: 39 1994 1712 138 Fragments arranged by size: 1994 1712 1534 1443 274 229 223 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4502 4534 4807 4839 7710 7742 4502 Size: 32 273 32 2871 32 4510 Fragments arranged by size: 4510 2871 273 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7750 XbaI T'CTAG_A Cuts at: 5086 5086 Size: 7750 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3940 4893 3940 Size: 953 6797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7750 XmaI C'CCGG_G Cuts at: 1870 5075 1870 Size: 3205 4545 XmnI GAAnn'nnTTC Cuts at: 2344 3222 5879 2344 Size: 878 2657 4215 Fragments arranged by size: 4215 2657 878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5558 276 Size: 53 83 186 936 4024 2468 Fragments arranged by size: 4024 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI