(Circular) (Six-base) MAPSORT of: pFN21AA1009.seq Check: 7896 from: 1 to: 8686 pFN21AA1009 8686 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4959 6496 278 Size: 53 83 186 936 3423 1537 2468 Fragments arranged by size: 3423 2468 1537 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3515 4227 6029 1937 Size: 1578 712 1802 4594 Fragments arranged by size: 4594 1802 1578 712 Acc65I G'GTAC_C Cuts at: 3232 6007 3232 Size: 2775 5911 AclI AA'CG_TT Cuts at: 1563 6813 7186 1563 Size: 5250 373 3063 Fragments arranged by size: 5250 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2960 3090 3222 3357 4601 5093 5375 5388 Size: 1328 130 132 135 1244 492 282 13 Cuts at: 5388 5637 6749 8133 8401 1632 Size: 249 1112 1384 268 1917 Fragments arranged by size: 1917 1384 1328 1244 1112 492 282 268 249 135 132 130 13 AflII C'TTAA_G Cuts at: 820 1017 2045 2068 820 Size: 197 1028 23 7438 Fragments arranged by size: 7438 1028 197 23 AflIII A'CryG_T Cuts at: 1203 2977 3091 7585 8562 1203 Size: 1774 114 4494 977 1327 Fragments arranged by size: 4494 1774 1327 977 114 AgeI A'CCGG_T Cuts at: 7517 7517 Size: 8686 AhdI GACnn_n'nnGTC Cuts at: 7415 7415 Size: 8686 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4827 1184 Size: 3643 5043 AlwNI CAG_nnn'CTG Cuts at: 3548 4546 4634 6429 8001 3548 Size: 998 88 1795 1572 4233 Fragments arranged by size: 4233 1795 1572 998 88 ApaLI G'TGCA_C Cuts at: 2385 3255 6743 7899 2385 Size: 870 3488 1156 3172 Fragments arranged by size: 3488 3172 1156 870 ApoI r'AATT_y Cuts at: 1493 2428 2773 2918 4123 4311 4536 4664 5000 Size: 935 345 145 1205 188 225 128 336 Cuts at: 5000 5497 5631 5995 6221 6373 8482 1493 Size: 497 134 364 226 152 2109 1697 Fragments arranged by size: 2109 1697 1205 935 497 364 345 336 226 225 188 152 145 134 128 AseI AT'TA_AT Cuts at: 160 1032 2280 3171 5333 7240 160 Size: 872 1248 891 2162 1907 1606 Fragments arranged by size: 2162 1907 1606 1248 891 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8686 AvaI C'yCGr_G Cuts at: 1870 1943 6011 1870 Size: 73 4068 4545 Fragments arranged by size: 4545 4068 73 AvrII C'CTAG_G Cuts at: 3428 3428 Size: 8686 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3577 3610 3577 Size: 33 8653 BamHI G'GATC_C Cuts at: 6016 6383 6016 Size: 367 8319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2571 3232 6007 7462 618 Size: 325 217 627 784 661 2775 1455 1842 Fragments arranged by size: 2775 1842 1455 784 661 627 325 217 BanII G_rGCy'C Cuts at: 1391 2115 2741 3314 4110 6005 1391 Size: 724 626 573 796 1895 4072 Fragments arranged by size: 4072 1895 796 724 626 573 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2700 4191 928 Size: 971 801 1491 5423 Fragments arranged by size: 5423 1491 971 801 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4055 4089 6877 6911 1903 Size: 34 2118 34 2788 34 3678 Fragments arranged by size: 3678 2788 2118 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 6578 7788 3578 Size: 3000 1210 4476 Fragments arranged by size: 4476 3000 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5177 7297 136 Size: 107 122 71 631 605 3505 2120 1525 Fragments arranged by size: 3505 2120 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3185 3973 4132 8681 3185 Size: 788 159 4549 3190 Fragments arranged by size: 4549 3190 788 159 BlpI GC'TnA_GC Cuts at: 6103 6103 Size: 8686 Bme1580I G_kGCm'C Cuts at: 1790 2389 2457 3259 6747 7903 1790 Size: 599 68 802 3488 1156 2573 Fragments arranged by size: 3488 2573 1156 802 599 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3324 6417 7375 Size: 416 53 83 399 24 1907 3093 958 Cuts at: 7375 442 Size: 1753 Fragments arranged by size: 3093 1907 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3894 4568 4724 5894 7346 1239 Size: 2655 674 156 1170 1452 2579 Fragments arranged by size: 2655 2579 1452 1170 674 156 Bpu10I CC'TnA_GC Cuts at: 2562 3313 2562 Size: 751 7935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 2742 2949 3326 3421 4071 4469 5185 5526 Size: 532 207 377 95 650 398 716 341 Cuts at: 5526 6811 7676 7974 2210 Size: 1285 865 298 2922 Fragments arranged by size: 2922 1285 865 716 650 532 398 377 341 298 207 95 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7349 882 Size: 617 5850 2219 Fragments arranged by size: 5850 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6477 493 Size: 711 5273 2702 Fragments arranged by size: 5273 2702 711 BsaBI GATnn'nnATC Cuts at: 8680 8680 Size: 8686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4956 6493 6875 7510 Size: 53 83 186 936 3423 1537 382 635 Cuts at: 7510 275 Size: 1451 Fragments arranged by size: 3423 1537 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7119 7517 7791 7938 8349 7119 Size: 398 274 147 411 7456 Fragments arranged by size: 7456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3995 4025 5928 5958 1178 Size: 30 165 30 2592 30 1903 30 3906 Fragments arranged by size: 3906 2592 1903 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2177 2834 4016 1186 Size: 847 144 657 1182 5856 Fragments arranged by size: 5856 1182 847 657 144 BseYI C'CCAG_C Cuts at: 7889 8385 7889 Size: 496 8190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5909 1163 Size: 573 4173 3940 Fragments arranged by size: 4173 3940 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6136 6897 7925 1540 Size: 275 186 4135 761 1028 2301 Fragments arranged by size: 4135 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 2389 2741 3259 3314 6747 6832 7903 2115 Size: 274 352 518 55 3433 85 1071 2898 Fragments arranged by size: 3433 2898 1071 518 352 274 85 55 BsmI GAATG_Cn' Cuts at: 4948 5977 6197 6290 4948 Size: 1029 220 93 7344 Fragments arranged by size: 7344 1029 220 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2389 2457 2741 3259 3314 4110 Size: 399 325 274 68 284 518 55 796 Cuts at: 4110 6005 6747 6832 7903 1391 Size: 1895 742 85 1071 2174 Fragments arranged by size: 2174 1895 1071 796 742 518 399 325 284 274 85 68 55 BspEI T'CCGG_A Cuts at: 8349 8349 Size: 8686 BspHI T'CATG_A Cuts at: 6575 8305 6575 Size: 1730 6956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4615 6041 844 Size: 3771 1426 3489 Fragments arranged by size: 3771 3489 1426 BsrBI CCG'CTC Cuts at: 1394 1823 6573 1394 Size: 429 4750 3507 Fragments arranged by size: 4750 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5430 7181 7355 65 Size: 1183 4182 1751 174 1396 Fragments arranged by size: 4182 1751 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5928 7330 7517 1700 Size: 110 4118 1402 187 2869 Fragments arranged by size: 4118 2869 1402 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8686 BssSI C'ACGA_G Cuts at: 6746 7758 6746 Size: 1012 7674 BstAPI GCAn_nnn'nTGC Cuts at: 794 4526 5432 794 Size: 3732 906 4048 Fragments arranged by size: 4048 3732 906 BstBI TT'CG_AA Cuts at: 4233 7490 4233 Size: 3257 5429 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8686 BstYI r'GATC_y Cuts at: 1972 3185 3973 4132 4543 6016 6383 6768 6785 Size: 1213 788 159 411 1473 367 385 17 Cuts at: 6785 8237 8323 8335 8681 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1473 1452 1213 788 411 385 367 346 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3580 3580 Size: 8686 BtgI C'CryG_G Cuts at: 513 1065 2004 4743 513 Size: 552 939 2739 4456 Fragments arranged by size: 4456 2739 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7947 Fragments arranged by size: 7947 683 56 BtsI GCAGTG_nn' Cuts at: 2059 5379 6202 6996 7016 2059 Size: 3320 823 794 20 3729 Fragments arranged by size: 3729 3320 823 794 20 ClaI AT'CG_AT Cuts at: 1554 3407 1554 Size: 1853 6833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5079 5114 529 Size: 35 4515 35 4101 Fragments arranged by size: 4515 4101 35 35 DraI TTT'AAA Cuts at: 2266 3625 5990 2266 Size: 1359 2365 4962 Fragments arranged by size: 4962 2365 1359 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7693 809 Size: 507 6377 1802 Fragments arranged by size: 6377 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2898 6133 7022 Size: 54 1425 50 275 18 1068 3235 889 Cuts at: 7022 8651 8 Size: 1629 43 Fragments arranged by size: 3235 1629 1425 1068 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6133 1537 Size: 275 4321 4090 Fragments arranged by size: 4321 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 2403 2763 3767 4577 6616 1353 Size: 657 393 360 1004 810 2039 3423 Fragments arranged by size: 3423 2039 1004 810 657 393 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7273 7647 7793 387 Size: 1064 138 5684 374 146 1280 Fragments arranged by size: 5684 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2113 2739 3312 2113 Size: 626 573 7487 Fragments arranged by size: 7487 626 573 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2960 3090 3222 3357 3894 4568 4601 Size: 393 1328 130 132 135 537 674 33 Cuts at: 4601 4724 5093 5375 5388 5637 5894 6749 7346 Size: 123 369 282 13 249 257 855 597 Cuts at: 7346 8133 8401 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1328 855 787 674 597 537 393 369 282 268 257 249 135 132 130 123 33 13 EcoNI CCTnn'n_nnAGG Cuts at: 4859 4859 Size: 8686 EcoRI G'AATT_C Cuts at: 2428 2773 5000 5995 6373 2428 Size: 345 2227 995 378 4741 Fragments arranged by size: 4741 2227 995 378 345 EcoRV GAT'ATC Cuts at: 2731 3935 4592 2731 Size: 1204 657 6825 Fragments arranged by size: 6825 1204 657 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3938 4421 4522 5567 3938 Size: 483 101 1045 7057 Fragments arranged by size: 7057 1045 483 101 FspI TGC'GCA Cuts at: 3663 7192 3663 Size: 3529 5157 HaeII r_GCGC'y Cuts at: 1678 7833 1678 Size: 6155 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2309 2341 4576 4608 4976 Size: 32 135 32 737 32 2235 32 368 Cuts at: 4976 5008 5150 5182 7327 7359 7401 7433 1373 Size: 32 142 32 2145 32 42 32 2626 Fragments arranged by size: 2626 2235 2145 737 368 142 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2394 6030 6276 669 Size: 988 281 456 3636 246 3079 Fragments arranged by size: 3636 3079 988 456 281 246 HindIII A'AGCT_T Cuts at: 748 2268 2658 748 Size: 1520 390 6776 Fragments arranged by size: 6776 1520 390 HpaI GTT'AAC Cuts at: 6276 6276 Size: 8686 KpnI G_GTAC'C Cuts at: 3236 6011 3236 Size: 2775 5911 MfeI C'AATT_G Cuts at: 6285 6285 Size: 8686 MluI A'CGCG_T Cuts at: 8562 8562 Size: 8686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3708 7800 7984 1305 Size: 2403 4092 184 2007 Fragments arranged by size: 4092 2403 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6864 Fragments arranged by size: 6864 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2319 4941 6644 7003 7162 518 Size: 648 1153 2622 1703 359 159 2042 Fragments arranged by size: 2622 2042 1703 1153 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3054 3860 4634 6102 6779 7927 8172 1606 Size: 1448 806 774 1468 677 1148 245 2120 Fragments arranged by size: 2120 1468 1448 1148 806 774 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4743 513 Size: 552 939 2739 4456 Fragments arranged by size: 4456 2739 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8686 NotI GC'GGCC_GC Cuts at: 6133 6133 Size: 8686 NspI r_CATG'y Cuts at: 1132 1229 2485 2981 3095 5756 6044 7589 1132 Size: 97 1256 496 114 2661 288 1545 2229 Fragments arranged by size: 2661 2229 1545 1256 496 288 114 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8686 PciI A'CATG_T Cuts at: 2977 3091 7585 2977 Size: 114 4494 4078 Fragments arranged by size: 4494 4078 114 PflMI CCAn_nnn'nTGG Cuts at: 1108 2683 1108 Size: 1575 7111 PmeI GTTT'AAAC Cuts at: 5990 5990 Size: 8686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6755 6787 8268 8300 6755 Size: 32 1481 32 7141 Fragments arranged by size: 7141 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8686 PsiI TTA'TAA Cuts at: 3386 5555 6256 3386 Size: 2169 701 5816 Fragments arranged by size: 5816 2169 701 PstI C_TGCA'G Cuts at: 830 3475 5425 5589 6038 830 Size: 2645 1950 164 449 3478 Fragments arranged by size: 3478 2645 1950 449 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8686 PvuII CAG'CTG Cuts at: 3054 3860 4634 3054 Size: 806 774 7106 Fragments arranged by size: 7106 806 774 SacI G_AGCT'C Cuts at: 2115 2741 3314 2115 Size: 626 573 7487 Fragments arranged by size: 7487 626 573 SalI G'TCGA_C Cuts at: 1936 6028 1936 Size: 4092 4594 SbfI CC_TGCA'GG Cuts at: 6038 6038 Size: 8686 ScaI AGT'ACT Cuts at: 2074 6934 8646 2074 Size: 4860 1712 2114 Fragments arranged by size: 4860 2114 1712 SfcI C'TryA_G Cuts at: 826 1045 3471 4995 5305 5421 5585 5636 6034 Size: 219 2426 1524 310 116 164 51 398 Cuts at: 6034 7169 7850 8041 826 Size: 1135 681 191 1471 Fragments arranged by size: 2426 1524 1471 1135 681 398 310 219 191 164 116 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8686 SmaI CCC'GGG Cuts at: 1872 6013 1872 Size: 4141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 2068 2189 2757 2964 3341 Size: 197 926 102 23 121 568 207 377 Cuts at: 3341 3400 4086 4448 5200 5541 6790 7691 7953 Size: 59 686 362 752 341 1249 901 262 Cuts at: 7953 820 Size: 1553 Fragments arranged by size: 1553 1249 926 901 752 686 568 377 362 341 262 207 197 121 102 59 23 SnaBI TAC'GTA Cuts at: 493 6477 493 Size: 5984 2702 SpeI A'CTAG_T Cuts at: 152 2098 152 Size: 1946 6740 SphI G_CATG'C Cuts at: 1132 2485 6044 1132 Size: 1353 3559 3774 Fragments arranged by size: 3774 3559 1353 SspI AAT'ATT Cuts at: 5 52 2956 3115 4617 5904 6610 5 Size: 47 2904 159 1502 1287 706 2081 Fragments arranged by size: 2904 2081 1502 1287 706 159 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8686 StyI C'CwwG_G Cuts at: 513 1065 2004 2567 3428 4743 6125 513 Size: 552 939 563 861 1315 1382 3074 Fragments arranged by size: 3074 1382 1315 939 861 563 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6713 6730 6883 7068 7513 325 Size: 1255 5133 17 153 185 445 1498 Fragments arranged by size: 5133 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2072 6932 8644 Size: 274 80 33 51 1443 95 4860 1712 Cuts at: 8644 96 Size: 138 Fragments arranged by size: 4860 1712 1443 274 138 95 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5420 5452 5928 5960 8646 8678 5420 Size: 32 476 32 2686 32 5428 Fragments arranged by size: 5428 2686 476 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8686 XbaI T'CTAG_A Cuts at: 6022 6022 Size: 8686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8686 XmaI C'CCGG_G Cuts at: 1870 6011 1870 Size: 4141 4545 XmnI GAAnn'nnTTC Cuts at: 2034 2845 4575 4986 5631 5708 6815 2034 Size: 811 1730 411 645 77 1107 3905 Fragments arranged by size: 3905 1730 1107 811 645 411 77 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4957 6494 276 Size: 53 83 186 936 3423 1537 2468 Fragments arranged by size: 3423 2468 1537 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI