Restriction map for
pFN21AA1006
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII AccII AclI AcyI AflII
AgeI AhdI AocI ApaI AscI
AspEI AspI AvrII BbeI BbrPI
BbvCI BcgI BfrI BlnI BsaAI
BsaHI BsiEI BsiWI BspDI BsrBI
BsrFI BssHII BssSI BstEII BstPI
BstUI Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
FseI HgaI Hin1I KasI KspI
MluI MvnI NaeI NarI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I StuI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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Acc65I 3548 GGTACC G^GTACC
AccI 2844 GTATAC GT^[AC][GT]AC
AccIII 1266 TCCGGA T^CCGGA
AflIII 1516 ACATGT A^C[GA][CT]GT
Alw44I 3413 GTGCAC G^TGCAC
Aor51HI 2225 AGCGCT AGC^GCT
ApaLI 3413 GTGCAC G^TGCAC
AseI 2721 ATTAAT AT^TAAT
AsnI 2721 ATTAAT AT^TAAT
Asp700I 98 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3548 GGTACC G^GTACC
AvaI 289 CTCGGG C^[CT]CG[GA]G
AviII 3336 TGCGCA TGC^GCA
BalI 1336 TGGCCA TGG^CCA
BanII 3292 GAGCTC G[GA]GC[CT]^C
BciVI 1318 GGATAC GTATCC(6/5)
BclI 359 TGATCA T^GATCA
BcnI 462 CCGGG CC^[GC]GG
BglI 2974 GCCTGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1943 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1943 GCTCAGC GC^T[ACGT]AGC
BsaBI 769 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1266 TCCGGA [AT]^CCGG[AT]
BseAI 1266 TCCGGA T^CCGGA
BsmBI 3126 CGTCTC CGTCTC(1/5)
BsmI 1853 GAATGC GAATGC(1/-1)
BsoBI 289 CTCGGG C^[CT]CG[GA]G
BspEI 1266 TCCGGA T^CCGGA
BspLU11I 1516 ACATGT A^CATGT
BspMI 943 ACCTGC ACCTGC(4/8)
Bst1107I 2844 GTATAC GTA^TAC
BstBI 2363 TTCGAA TT^CGAA
BstZ17I 2844 GTATAC GTA^TAC
CelII 1943 GCTCAGC GC^T[ACGT]AGC
DraII 2343 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eco47III 2225 AGCGCT AGC^GCT
EcoNI 3349 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2343 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1865 GATATC GAT^ATC
EcoT22I 1027 ATGCAT ATGCA^T
FbaI 359 TGATCA T^GATCA
FspI 3336 TGCGCA TGC^GCA
HincII 3150 GTTAAC GT[CT]^[GA]AC
HindII 3150 GTTAAC GT[CT]^[GA]AC
HpaI 3150 GTTAAC GTT^AAC
KpnI 3548 GGTACC GGTAC^C
MaeII 2304 ACGT A^CGT
MamI 769 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1336 TGGCCA TGG^CCA
MroI 1266 TCCGGA T^CCGGA
MscI 1336 TGGCCA TGG^CCA
NciI 462 CCGGG CC^[GC]GG
NcoI 1211 CCATGG C^CATGG
NsiI 1027 ATGCAT ATGCA^T
NspV 2363 TTCGAA TT^CGAA
PflMI 1339 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2343 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2721 ATTAAT AT^TAAT
PvuII 335 CAGCTG CAG^CTG
SacI 3292 GAGCTC GAGCT^C
SapI 3289 GAAGAGC GCTCTTC(1/4)
SfuI 2363 TTCGAA TT^CGAA
SpeI 1927 ACTAGT A^CTAGT
Tsp45I 2054 GTGAC ^GT[GC]AC
Van91I 1339 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2446 TCTAGA T^CTAGA
XmnI 98 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 1450 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2970 CAGTGCCTG
BamHI 2162 GGATCC G^GATCC
2751 GGATCC
BanI 370 GGTGCC G^G[CT][GA]CC
3548 GGTACC
BglII 1152 AGATCT A^GATCT
1734 AGATCT
BsaI 294 GAGACC GGTCTC(1/5)
2078 GGTCTC
Bsp1407I 633 TGTACA T^GTACA
1457 TGTACA
BsrDI 2103 CATTGC GCAATG(2/0)
1976 GCAATG
BsrGI 633 TGTACA T^GTACA
1457 TGTACA
BstXI 804 CCATTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2250 CCATGTAAATGG
DsaI 1211 CCATGG C^C[GA][CT]GG
2074 CCACGG
EaeI 1336 TGGCCA [CT]^GGCC[GA]
1843 CGGCCA
EarI 3289 GAAGAG CTCTTC(1/4)
3460 GAAGAG
FauI 3326 CCCGC CCCGC(4/6)
118 GCGGG
HaeII 2225 AGCGCT [GA]GCGC^[CT]
3379 GGCGCT
HindIII 998 AAGCTT A^AGCTT
3305 AAGCTT
Ksp632I 3289 GAAGAG CTCTTC(1/4)
3460 GAAGAG
MspA1I 335 CAGCTG C[AC]G^C[GT]G
3145 CAGCGG
SexAI 297 ACCTGGT A^CC[AT]GGT
3354 ACCAGGT
SmlI 2221 CTTGAG C^T[CT][GA]AG
3247 CTCAAG
SphI 2120 GCATGC GCATG^C
2983 GCATGC
SspBI 633 TGTACA T^GTACA
1457 TGTACA
TaqI 2085 TCGA T^CGA
2364 TCGA
TthHB8I 2085 TCGA T^CGA
2364 TCGA
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspI MunI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SspI StyI TfiI Tru9I TseI
Tsp509I TspRI XcmI XhoII