(Circular) (Six-base) MAPSORT of: pFN21AA1006.seq Check: 8990 from: 1 to: 8263 pFN21AA1006 8263 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6073 278 Size: 53 83 186 936 4537 2468 Fragments arranged by size: 4537 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4850 5606 1937 Size: 2913 756 4594 Fragments arranged by size: 4594 2913 756 Acc65I G'GTAC_C Cuts at: 5553 5584 5553 Size: 31 8232 AclI AA'CG_TT Cuts at: 1563 6390 6763 1563 Size: 4827 373 3063 Fragments arranged by size: 4827 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3665 6326 7710 7978 1632 Size: 2033 2661 1384 268 1917 Fragments arranged by size: 2661 2033 1917 1384 268 AfeI AGC'GCT Cuts at: 4232 4232 Size: 8263 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8066 AflIII A'CryG_T Cuts at: 1203 3521 7162 8139 1203 Size: 2318 3641 977 1327 Fragments arranged by size: 3641 2318 1327 977 AgeI A'CCGG_T Cuts at: 7094 7094 Size: 8263 AhdI GACnn_n'nnGTC Cuts at: 6992 6992 Size: 8263 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8263 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8263 AlwNI CAG_nnn'CTG Cuts at: 3460 4980 6006 7578 3460 Size: 1520 1026 1572 4145 Fragments arranged by size: 4145 1572 1520 1026 ApaLI G'TGCA_C Cuts at: 5418 6320 7476 5418 Size: 902 1156 6205 Fragments arranged by size: 6205 1156 902 ApoI r'AATT_y Cuts at: 1493 2151 2459 3080 3116 3425 3629 4435 5145 Size: 658 308 621 36 309 204 806 710 Cuts at: 5145 5572 5798 5950 8059 1493 Size: 427 226 152 2109 1697 Fragments arranged by size: 2109 1697 806 710 658 621 427 309 308 226 204 152 36 AseI AT'TA_AT Cuts at: 160 1032 4727 6817 160 Size: 872 3695 2090 1606 Fragments arranged by size: 3695 2090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8263 AvaI C'yCGr_G Cuts at: 1870 1943 2294 5588 1870 Size: 73 351 3294 4545 Fragments arranged by size: 4545 3294 351 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2688 2721 2688 Size: 33 8230 BamHI G'GATC_C Cuts at: 4167 4756 5593 5960 4167 Size: 589 837 367 6470 Fragments arranged by size: 6470 837 589 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2375 5553 5584 7039 618 Size: 325 217 627 588 3178 31 1455 1842 Fragments arranged by size: 3178 1842 1455 627 588 325 217 31 BanII G_rGCy'C Cuts at: 1391 5301 5582 1391 Size: 3910 281 4072 Fragments arranged by size: 4072 3910 281 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3019 3546 3848 4019 4363 4876 5027 Size: 971 1120 527 302 171 344 513 151 Cuts at: 5027 928 Size: 4164 Fragments arranged by size: 4164 1120 971 527 513 344 302 171 151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6454 6488 1903 Size: 34 4517 34 3678 Fragments arranged by size: 4517 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3317 6155 7365 3317 Size: 2838 1210 4215 Fragments arranged by size: 4215 2838 1210 BclI T'GATC_A Cuts at: 1548 2364 1548 Size: 816 7447 BfrBI ATG'CAT Cuts at: 3034 3034 Size: 8263 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4985 6874 136 Size: 107 122 71 631 605 3313 1889 1525 Fragments arranged by size: 3313 1889 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3157 3739 8258 3157 Size: 582 4519 3162 Fragments arranged by size: 4519 3162 582 BlpI GC'TnA_GC Cuts at: 3949 5680 3949 Size: 1731 6532 Bme1580I G_kGCm'C Cuts at: 1790 5422 6324 7480 1790 Size: 3632 902 1156 2573 Fragments arranged by size: 3632 2573 1156 902 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8263 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4372 5994 6952 Size: 416 53 83 399 24 2955 1622 958 Cuts at: 6952 442 Size: 1753 Fragments arranged by size: 2955 1753 1622 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8263 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5267 5315 5267 Size: 48 8215 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3170 3849 4453 5412 6923 1239 Size: 1931 679 604 959 1511 2579 Fragments arranged by size: 2579 1931 1511 959 679 604 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4247 5237 6388 7253 7551 4247 Size: 990 1151 865 298 4959 Fragments arranged by size: 4959 1151 990 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2293 4089 6926 882 Size: 617 794 1796 2837 2219 Fragments arranged by size: 2837 2219 1796 794 617 BsaAI yAC'GTr Cuts at: 493 1204 6054 493 Size: 711 4850 2702 Fragments arranged by size: 4850 2702 711 BsaBI GATnn'nnATC Cuts at: 2778 8257 2778 Size: 5479 2784 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6070 6452 7087 275 Size: 53 83 186 936 4537 382 635 1451 Fragments arranged by size: 4537 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3271 6696 7094 7368 7515 7926 3271 Size: 3425 398 274 147 411 3608 Fragments arranged by size: 3608 3425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2290 2320 3712 3742 4537 Size: 30 165 30 887 30 1392 30 795 Cuts at: 4537 4567 1178 Size: 30 4874 Fragments arranged by size: 4874 1392 887 795 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 3369 4077 1186 Size: 855 1328 708 5372 Fragments arranged by size: 5372 1328 855 708 BseYI C'CCAG_C Cuts at: 3750 4344 7466 7962 3750 Size: 594 3122 496 4051 Fragments arranged by size: 4051 3122 594 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2358 2824 4255 5514 1163 Size: 573 622 466 1431 1259 3912 Fragments arranged by size: 3912 1431 1259 622 573 466 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5713 6474 7502 1540 Size: 275 186 3712 761 1028 2301 Fragments arranged by size: 3712 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3376 3785 5301 5422 6324 6409 7480 3376 Size: 409 1516 121 902 85 1071 4159 Fragments arranged by size: 4159 1516 1071 902 409 121 85 BsmI GAATG_Cn' Cuts at: 3864 5774 5867 3864 Size: 1910 93 6260 Fragments arranged by size: 6260 1910 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5137 5137 Size: 8263 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3376 3785 5301 5422 5582 6324 6409 Size: 399 1586 409 1516 121 160 742 85 Cuts at: 6409 7480 1391 Size: 1071 2174 Fragments arranged by size: 2174 1586 1516 1071 742 409 399 160 121 85 BspEI T'CCGG_A Cuts at: 3271 7926 3271 Size: 4655 3608 BspHI T'CATG_A Cuts at: 2782 3226 5013 6152 7882 2782 Size: 444 1787 1139 1730 3163 Fragments arranged by size: 3163 1787 1730 1139 444 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 5618 844 Size: 2113 2661 3489 Fragments arranged by size: 3489 2661 2113 BsrBI CCG'CTC Cuts at: 1394 1823 6150 1394 Size: 429 4327 3507 Fragments arranged by size: 4327 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3988 4107 6758 6932 65 Size: 1183 2740 119 2651 174 1396 Fragments arranged by size: 2740 2651 1396 1183 174 119 BsrFI r'CCGG_y Cuts at: 1700 1810 6907 7094 1700 Size: 110 5097 187 2869 Fragments arranged by size: 5097 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2638 3462 96 Size: 2542 824 4897 Fragments arranged by size: 4897 2542 824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8263 BssSI C'ACGA_G Cuts at: 6323 7335 6323 Size: 1012 7251 BstAPI GCAn_nnn'nTGC Cuts at: 794 3257 4980 5426 794 Size: 2463 1723 446 3631 Fragments arranged by size: 3631 2463 1723 446 BstBI TT'CG_AA Cuts at: 4369 7067 4369 Size: 2698 5565 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8263 BstXI CCAn_nnnn'nTGG Cuts at: 2816 4262 2816 Size: 1446 6817 BstYI r'GATC_y Cuts at: 1972 3157 3739 4167 4756 5593 5960 6345 6362 Size: 1185 582 428 589 837 367 385 17 Cuts at: 6362 7814 7900 7912 8258 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1185 837 589 582 428 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4851 4851 Size: 8263 BtgI C'CryG_G Cuts at: 513 1065 3216 4079 513 Size: 552 2151 863 4697 Fragments arranged by size: 4697 2151 863 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7524 Fragments arranged by size: 7524 683 56 BtsI GCAGTG_nn' Cuts at: 2068 2080 4267 4889 4981 5779 6573 6593 2068 Size: 12 2187 622 92 798 794 20 3738 Fragments arranged by size: 3738 2187 798 794 622 92 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8263 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3881 3916 4941 4976 529 Size: 35 3317 35 1025 35 3816 Fragments arranged by size: 3816 3317 1025 35 35 35 DraI TTT'AAA Cuts at: 5567 5567 Size: 8263 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7270 809 Size: 507 5954 1802 Fragments arranged by size: 5954 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3341 3848 5710 Size: 54 1425 50 275 18 1511 507 1862 Cuts at: 5710 6599 8228 8 Size: 889 1629 43 Fragments arranged by size: 1862 1629 1511 1425 889 507 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5710 1537 Size: 275 3898 4090 Fragments arranged by size: 4090 3898 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5289 5460 6193 1353 Size: 3936 171 733 3423 Fragments arranged by size: 3936 3423 733 171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6850 7224 7370 387 Size: 1064 138 5261 374 146 1280 Fragments arranged by size: 5261 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5299 5299 Size: 8263 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3170 3665 3849 4453 5412 6326 6923 Size: 393 1538 495 184 604 959 914 597 Cuts at: 6923 7710 7978 1239 Size: 787 268 1524 Fragments arranged by size: 1538 1524 959 914 787 604 597 495 393 268 184 EcoNI CCTnn'n_nnAGG Cuts at: 5358 5358 Size: 8263 EcoO109I rG'GnC_Cy Cuts at: 4349 4349 Size: 8263 EcoRI G'AATT_C Cuts at: 5572 5950 5572 Size: 378 7885 EcoRV GAT'ATC Cuts at: 3872 3872 Size: 8263 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2874 3899 4299 2874 Size: 1025 400 6838 Fragments arranged by size: 6838 1025 400 FspI TGC'GCA Cuts at: 5343 6769 5343 Size: 1426 6837 HaeII r_GCGC'y Cuts at: 1678 4234 5388 7410 1678 Size: 2556 1154 2022 2531 Fragments arranged by size: 2556 2531 2022 1154 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2568 2600 2705 2737 3919 Size: 32 135 32 996 32 105 32 1182 Cuts at: 3919 3951 6904 6936 6978 7010 1373 Size: 32 2953 32 42 32 2626 Fragments arranged by size: 2953 2626 1182 996 135 105 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5157 5607 5853 669 Size: 988 281 3219 450 246 3079 Fragments arranged by size: 3219 3079 988 450 281 246 HindIII A'AGCT_T Cuts at: 748 3003 5310 748 Size: 2255 2307 3701 Fragments arranged by size: 3701 2307 2255 HpaI GTT'AAC Cuts at: 5157 5853 5157 Size: 696 7567 KpnI G_GTAC'C Cuts at: 5557 5588 5557 Size: 31 8232 MfeI C'AATT_G Cuts at: 2601 2769 3893 5862 2601 Size: 168 1124 1969 5002 Fragments arranged by size: 5002 1969 1124 168 MluI A'CGCG_T Cuts at: 8139 8139 Size: 8263 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2500 4304 4549 5028 7377 7561 1305 Size: 1195 1804 245 479 2349 184 2007 Fragments arranged by size: 2349 2007 1804 1195 479 245 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3343 10 Size: 54 1425 343 1511 4930 Fragments arranged by size: 4930 1511 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2331 2814 3411 3557 4260 5018 5517 Size: 648 1165 483 597 146 703 758 499 Cuts at: 5517 6221 6580 6739 518 Size: 704 359 159 2042 Fragments arranged by size: 2042 1165 758 704 703 648 597 499 483 359 159 146 MspA1I CmG'CkG Cuts at: 1606 2342 5152 5679 6356 7504 7749 1606 Size: 736 2810 527 677 1148 245 2120 Fragments arranged by size: 2810 2120 1148 736 677 527 245 NcoI C'CATG_G Cuts at: 513 1065 3216 513 Size: 552 2151 5560 Fragments arranged by size: 5560 2151 552 NdeI CA'TA_TG Cuts at: 387 3613 3955 4741 387 Size: 3226 342 786 3909 Fragments arranged by size: 3909 3226 786 342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8263 NotI GC'GGCC_GC Cuts at: 5710 5710 Size: 8263 NsiI A_TGCA'T Cuts at: 3036 3036 Size: 8263 NspI r_CATG'y Cuts at: 1132 1229 3246 3525 4129 4992 5197 5516 5621 Size: 97 2017 279 604 863 205 319 105 Cuts at: 5621 7166 1132 Size: 1545 2229 Fragments arranged by size: 2229 2017 1545 863 604 319 279 205 105 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8263 PciI A'CATG_T Cuts at: 3521 7162 3521 Size: 3641 4622 PflMI CCAn_nnn'nTGG Cuts at: 1108 3350 1108 Size: 2242 6021 PfoI T'CCnGG_A Cuts at: 3865 4351 5353 3865 Size: 486 1002 6775 Fragments arranged by size: 6775 1002 486 PmeI GTTT'AAAC Cuts at: 5567 5567 Size: 8263 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4282 4314 6332 6364 7845 7877 4282 Size: 32 2018 32 1481 32 4668 Fragments arranged by size: 4668 2018 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4349 4349 Size: 8263 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8263 PsiI TTA'TAA Cuts at: 3627 5833 3627 Size: 2206 6057 PstI C_TGCA'G Cuts at: 830 2134 3456 5479 5615 830 Size: 1304 1322 2023 136 3478 Fragments arranged by size: 3478 2023 1322 1304 136 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8263 PvuII CAG'CTG Cuts at: 2342 2342 Size: 8263 SacI G_AGCT'C Cuts at: 5301 5301 Size: 8263 SalI G'TCGA_C Cuts at: 1936 5605 1936 Size: 3669 4594 SapI GCTCTTCn'nnn_ Cuts at: 5289 5289 Size: 8263 SbfI CC_TGCA'GG Cuts at: 5615 5615 Size: 8263 ScaI AGT'ACT Cuts at: 3054 3288 4020 6511 8223 3054 Size: 234 732 2491 1712 3094 Fragments arranged by size: 3094 2491 1712 732 234 SexAI A'CCwGG_T Cuts at: 2302 5359 2302 Size: 3057 5206 SfcI C'TryA_G Cuts at: 826 1045 2130 2348 2700 3297 3452 5186 5475 Size: 219 1085 218 352 597 155 1734 289 Cuts at: 5475 5611 6746 7427 7618 826 Size: 136 1135 681 191 1471 Fragments arranged by size: 1734 1471 1135 1085 681 597 352 289 219 218 191 155 136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8263 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8263 SmaI CCC'GGG Cuts at: 1872 5590 1872 Size: 3718 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4226 5252 6367 7268 7530 820 Size: 197 926 2283 1026 1115 901 262 1553 Fragments arranged by size: 2283 1553 1115 1026 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6054 493 Size: 5561 2702 SpeI A'CTAG_T Cuts at: 152 3932 152 Size: 3780 4483 SphI G_CATG'C Cuts at: 1132 4129 4992 5621 1132 Size: 2997 863 629 3774 Fragments arranged by size: 3774 2997 863 629 SspI AAT'ATT Cuts at: 5 52 2554 2976 3588 6187 5 Size: 47 2502 422 612 2599 2081 Fragments arranged by size: 2599 2502 2081 612 422 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8263 StyI C'CwwG_G Cuts at: 513 1065 2379 3216 3367 5702 513 Size: 552 1314 837 151 2335 3074 Fragments arranged by size: 3074 2335 1314 837 552 151 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4709 4728 6290 6307 6460 6645 7090 Size: 1255 3129 19 1562 17 153 185 445 Cuts at: 7090 325 Size: 1498 Fragments arranged by size: 3129 1562 1498 1255 445 185 153 19 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2638 3052 3286 Size: 274 80 33 51 1443 661 414 234 Cuts at: 3286 3462 4018 6509 8221 96 Size: 176 556 2491 1712 138 Fragments arranged by size: 2491 1712 1443 661 556 414 274 234 176 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2290 2322 8223 8255 2290 Size: 32 5901 32 2298 Fragments arranged by size: 5901 2298 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8263 XbaI T'CTAG_A Cuts at: 4451 5599 4451 Size: 1148 7115 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2330 4582 4746 2330 Size: 2252 164 5847 Fragments arranged by size: 5847 2252 164 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8263 XmaI C'CCGG_G Cuts at: 1870 5588 1870 Size: 3718 4545 XmnI GAAnn'nnTTC Cuts at: 2107 6392 2107 Size: 4285 3978 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6071 276 Size: 53 83 186 936 4537 2468 Fragments arranged by size: 4537 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI