Restriction map for
pFN21AA1006
(Linear) (Six-base) MAPSORT of: KIAA1006.seq Check: 2770 from: 1 to: 3558
KIAA1006.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AccI GT'mk_AC
Cuts at: 0 2845 3558
Size: 2845 713
Acc65I G'GTAC_C
Cuts at: 0 3548 3558
Size: 3548 10
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1660 3558
Size: 1660 1898
AfeI AGC'GCT
Cuts at: 0 2227 3558
Size: 2227 1331
AflIII A'CryG_T
Cuts at: 0 1516 3558
Size: 1516 2042
AlwNI CAG_nnn'CTG
Cuts at: 0 1455 2975 3558
Size: 1455 1520 583
Fragments arranged by size:
1520 1455 583
ApaLI G'TGCA_C
Cuts at: 0 3413 3558
Size: 3413 145
ApoI r'AATT_y
Cuts at: 0 146 454 1075 1111 1420 1624 2430 3140
Size: 146 308 621 36 309 204 806 710
Cuts at: 3140 3558
Size: 418
Fragments arranged by size:
806 710 621 418 309 308 204 146
36
AseI AT'TA_AT
Cuts at: 0 2722 3558
Size: 2722 836
AvaI C'yCGr_G
Cuts at: 0 289 3558
Size: 289 3269
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 683 716 3558
Size: 683 33 2842
Fragments arranged by size:
2842 683 33
BamHI G'GATC_C
Cuts at: 0 2162 2751 3558
Size: 2162 589 807
Fragments arranged by size:
2162 807 589
BanI G'GyrC_C
Cuts at: 0 370 3548 3558
Size: 370 3178 10
Fragments arranged by size:
3178 370 10
BanII G_rGCy'C
Cuts at: 0 3296 3558
Size: 3296 262
BbsI GAAGACnn'nnnn_
Cuts at: 0 1014 1541 1843 2014 2358 2871 3022 3558
Size: 1014 527 302 171 344 513 151 536
Fragments arranged by size:
1014 536 527 513 344 302 171 151
BciVI GTATCCnnnnn_n'
Cuts at: 0 1312 3558
Size: 1312 2246
BclI T'GATC_A
Cuts at: 0 359 3558
Size: 359 3199
BfrBI ATG'CAT
Cuts at: 0 1029 3558
Size: 1029 2529
BglI GCCn_nnn'nGGC
Cuts at: 0 2980 3558
Size: 2980 578
BglII A'GATC_T
Cuts at: 0 1152 1734 3558
Size: 1152 582 1824
Fragments arranged by size:
1824 1152 582
BlpI GC'TnA_GC
Cuts at: 0 1944 3558
Size: 1944 1614
Bme1580I G_kGCm'C
Cuts at: 0 3417 3558
Size: 3417 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 2367 3558
Size: 2367 1191
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3262 3310 3558
Size: 3262 48 248
Fragments arranged by size:
3262 248 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1844 2448 3407 3558
Size: 1165 679 604 959 151
Fragments arranged by size:
1165 959 679 604 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2242 3232 3558
Size: 2242 990 326
Fragments arranged by size:
2242 990 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 288 2084 3558
Size: 288 1796 1474
Fragments arranged by size:
1796 1474 288
BsaBI GATnn'nnATC
Cuts at: 0 773 3558
Size: 773 2785
BsaWI w'CCGG_w
Cuts at: 0 1266 3558
Size: 1266 2292
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 285 315 1707 1737 2532 2562 3558
Size: 285 30 1392 30 795 30 996
Fragments arranged by size:
1392 996 795 285 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 36 1364 2072 3558
Size: 36 1328 708 1486
Fragments arranged by size:
1486 1328 708 36
BseYI C'CCAG_C
Cuts at: 0 1745 2339 3558
Size: 1745 594 1219
Fragments arranged by size:
1745 1219 594
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 819 2250 3509 3558
Size: 353 466 1431 1259 49
Fragments arranged by size:
1431 1259 466 353 49
BsiHKAI G_wGCw'C
Cuts at: 0 1371 1780 3296 3417 3558
Size: 1371 409 1516 121 141
Fragments arranged by size:
1516 1371 409 141 121
BsmI GAATG_Cn'
Cuts at: 0 1859 3558
Size: 1859 1699
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3132 3558
Size: 3132 426
Bsp1286I G_dGCh'C
Cuts at: 0 1371 1780 3296 3417 3558
Size: 1371 409 1516 121 141
Fragments arranged by size:
1516 1371 409 141 121
BspEI T'CCGG_A
Cuts at: 0 1266 3558
Size: 1266 2292
BspHI T'CATG_A
Cuts at: 0 777 1221 3008 3558
Size: 777 444 1787 550
Fragments arranged by size:
1787 777 550 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 952 3558
Size: 952 2606
BsrDI GCAATG_nn'
Cuts at: 0 1983 2102 3558
Size: 1983 119 1456
Fragments arranged by size:
1983 1456 119
BsrGI T'GTAC_A
Cuts at: 0 633 1457 3558
Size: 633 824 2101
Fragments arranged by size:
2101 824 633
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1252 2975 3421 3558
Size: 1252 1723 446 137
Fragments arranged by size:
1723 1252 446 137
BstBI TT'CG_AA
Cuts at: 0 2364 3558
Size: 2364 1194
BstXI CCAn_nnnn'nTGG
Cuts at: 0 811 2257 3558
Size: 811 1446 1301
Fragments arranged by size:
1446 1301 811
BstYI r'GATC_y
Cuts at: 0 1152 1734 2162 2751 3558
Size: 1152 582 428 589 807
Fragments arranged by size:
1152 807 589 582 428
BstZ17I GTA'TAC
Cuts at: 0 2846 3558
Size: 2846 712
BtgI C'CryG_G
Cuts at: 0 1211 2074 3558
Size: 1211 863 1484
Fragments arranged by size:
1484 1211 863
BtsI GCAGTG_nn'
Cuts at: 0 63 75 2262 2884 2976 3558
Size: 63 12 2187 622 92 582
Fragments arranged by size:
2187 622 582 92 63 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1876 1911 2936 2971 3558
Size: 1876 35 1025 35 587
Fragments arranged by size:
1876 1025 587 35 35
EaeI y'GGCC_r
Cuts at: 0 1336 1843 3558
Size: 1336 507 1715
Fragments arranged by size:
1715 1336 507
EarI CTCTTCn'nnn_
Cuts at: 0 3284 3455 3558
Size: 3284 171 103
Fragments arranged by size:
3284 171 103
EcoICRI GAG'CTC
Cuts at: 0 3294 3558
Size: 3294 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1660 1844 2448 3407 3558
Size: 1165 495 184 604 959 151
Fragments arranged by size:
1165 959 604 495 184 151
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3353 3558
Size: 3353 205
EcoO109I rG'GnC_Cy
Cuts at: 0 2344 3558
Size: 2344 1214
EcoRV GAT'ATC
Cuts at: 0 1867 3558
Size: 1867 1691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 869 1894 2294 3558
Size: 869 1025 400 1264
Fragments arranged by size:
1264 1025 869 400
FspI TGC'GCA
Cuts at: 0 3338 3558
Size: 3338 220
HaeII r_GCGC'y
Cuts at: 0 2229 3383 3558
Size: 2229 1154 175
Fragments arranged by size:
2229 1154 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 563 595 700 732 1914 1946 3558
Size: 563 32 105 32 1182 32 1612
Fragments arranged by size:
1612 1182 563 105 32 32 32
HincII GTy'rAC
Cuts at: 0 3152 3558
Size: 3152 406
HindIII A'AGCT_T
Cuts at: 0 998 3305 3558
Size: 998 2307 253
Fragments arranged by size:
2307 998 253
HpaI GTT'AAC
Cuts at: 0 3152 3558
Size: 3152 406
KpnI G_GTAC'C
Cuts at: 0 3552 3558
Size: 3552 6
MfeI C'AATT_G
Cuts at: 0 596 764 1888 3558
Size: 596 168 1124 1670
Fragments arranged by size:
1670 1124 596 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 2299 2544 3023 3558
Size: 495 1804 245 479 535
Fragments arranged by size:
1804 535 495 479 245
MscI TGG'CCA
Cuts at: 0 1338 3558
Size: 1338 2220
MslI CAynn'nnrTG
Cuts at: 0 326 809 1406 1552 2255 3013 3512 3558
Size: 326 483 597 146 703 758 499 46
Fragments arranged by size:
758 703 597 499 483 326 146 46
MspA1I CmG'CkG
Cuts at: 0 337 3147 3558
Size: 337 2810 411
Fragments arranged by size:
2810 411 337
NcoI C'CATG_G
Cuts at: 0 1211 3558
Size: 1211 2347
NdeI CA'TA_TG
Cuts at: 0 1608 1950 2736 3558
Size: 1608 342 786 822
Fragments arranged by size:
1608 822 786 342
NsiI A_TGCA'T
Cuts at: 0 1031 3558
Size: 1031 2527
NspI r_CATG'y
Cuts at: 0 1241 1520 2124 2987 3192 3511 3558
Size: 1241 279 604 863 205 319 47
Fragments arranged by size:
1241 863 604 319 279 205 47
PciI A'CATG_T
Cuts at: 0 1516 3558
Size: 1516 2042
PflMI CCAn_nnn'nTGG
Cuts at: 0 1345 3558
Size: 1345 2213
PfoI T'CCnGG_A
Cuts at: 0 1860 2346 3348 3558
Size: 1860 486 1002 210
Fragments arranged by size:
1860 1002 486 210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2277 2309 3558
Size: 2277 32 1249
Fragments arranged by size:
2277 1249 32
PpuMI rG'GwC_Cy
Cuts at: 0 2344 3558
Size: 2344 1214
PsiI TTA'TAA
Cuts at: 0 1622 3558
Size: 1622 1936
PstI C_TGCA'G
Cuts at: 0 129 1451 3474 3558
Size: 129 1322 2023 84
Fragments arranged by size:
2023 1322 129 84
PvuII CAG'CTG
Cuts at: 0 337 3558
Size: 337 3221
SacI G_AGCT'C
Cuts at: 0 3296 3558
Size: 3296 262
SapI GCTCTTCn'nnn_
Cuts at: 0 3284 3558
Size: 3284 274
ScaI AGT'ACT
Cuts at: 0 1049 1283 2015 3558
Size: 1049 234 732 1543
Fragments arranged by size:
1543 1049 732 234
SexAI A'CCwGG_T
Cuts at: 0 297 3354 3558
Size: 297 3057 204
Fragments arranged by size:
3057 297 204
SfcI C'TryA_G
Cuts at: 0 125 343 695 1292 1447 3181 3470 3558
Size: 125 218 352 597 155 1734 289 88
Fragments arranged by size:
1734 597 352 289 218 155 125 88
SmlI C'TyrA_G
Cuts at: 0 2221 3247 3558
Size: 2221 1026 311
Fragments arranged by size:
2221 1026 311
SpeI A'CTAG_T
Cuts at: 0 1927 3558
Size: 1927 1631
SphI G_CATG'C
Cuts at: 0 2124 2987 3558
Size: 2124 863 571
Fragments arranged by size:
2124 863 571
SspI AAT'ATT
Cuts at: 0 549 971 1583 3558
Size: 549 422 612 1975
Fragments arranged by size:
1975 612 549 422
StyI C'CwwG_G
Cuts at: 0 374 1211 1362 3558
Size: 374 837 151 2196
Fragments arranged by size:
2196 837 374 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2704 2723 3558
Size: 2704 19 835
Fragments arranged by size:
2704 835 19
TatI w'GTAC_w
Cuts at: 0 633 1047 1281 1457 2013 3558
Size: 633 414 234 176 556 1545
Fragments arranged by size:
1545 633 556 414 234 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 285 317 3558
Size: 285 32 3241
Fragments arranged by size:
3241 285 32
XbaI T'CTAG_A
Cuts at: 0 2446 3558
Size: 2446 1112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 325 2577 2741 3558
Size: 325 2252 164 817
Fragments arranged by size:
2252 817 325 164
XmnI GAAnn'nnTTC
Cuts at: 0 102 3558
Size: 102 3456
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AlwNI ApaLI ApoI
AseI AvaI BaeI BamHI BanI BanII BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MmeI MscI MslI MspA1I NcoI NdeI
NsiI NspI PciI PflMI PfoI PpiI PpuMI PsiI
PstI PvuII SacI SapI ScaI SexAI SfcI SmlI
SpeI SphI SspI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AsiSI AvrII BbeI BbvCI BcgI
BmgBI BmtI Bpu10I BsaAI BsaHI BsiEI BsiWI BsrBI
BsrFI BssHII BssSI BstEII Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EciI EcoRI FseI FspAI KasI
MluI NaeI NarI NgoMIV NheI NotI NruI PacI
PasI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI StuI SwaI Tth111I XhoI
XmaI ZraI