(Circular) (Six-base) MAPSORT of: pFN21AA0917.seq Check: 9067 from: 1 to: 6631 pFN21AA0917 6631 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4441 278 Size: 53 83 186 936 2905 2468 Fragments arranged by size: 2905 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2271 3974 1937 Size: 334 1703 4594 Fragments arranged by size: 4594 1703 334 Acc65I G'GTAC_C Cuts at: 3952 3952 Size: 6631 AclI AA'CG_TT Cuts at: 1563 4758 5131 1563 Size: 3195 373 3063 Fragments arranged by size: 3195 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2310 2880 4694 6078 6346 1632 Size: 678 570 1814 1384 268 1917 Fragments arranged by size: 1917 1814 1384 678 570 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6434 AflIII A'CryG_T Cuts at: 1203 5530 6507 1203 Size: 4327 977 1327 Fragments arranged by size: 4327 1327 977 AgeI A'CCGG_T Cuts at: 5462 5462 Size: 6631 AhdI GACnn_n'nnGTC Cuts at: 5360 5360 Size: 6631 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6631 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6631 AlwNI CAG_nnn'CTG Cuts at: 3338 4374 5946 3338 Size: 1036 1572 4023 Fragments arranged by size: 4023 1572 1036 ApaLI G'TGCA_C Cuts at: 2846 4688 5844 2846 Size: 1842 1156 3633 Fragments arranged by size: 3633 1842 1156 ApoI r'AATT_y Cuts at: 1493 2086 2353 2975 3940 4166 4318 6427 1493 Size: 593 267 622 965 226 152 2109 1697 Fragments arranged by size: 2109 1697 965 622 593 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 5185 160 Size: 872 4153 1606 Fragments arranged by size: 4153 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 AvaI C'yCGr_G Cuts at: 1870 1943 3956 1870 Size: 73 2013 4545 Fragments arranged by size: 4545 2013 73 BamHI G'GATC_C Cuts at: 3961 4328 3961 Size: 367 6264 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3952 5407 618 Size: 325 217 627 2165 1455 1842 Fragments arranged by size: 2165 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3950 1391 Size: 2559 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5660 BbvCI CC'TCA_GC Cuts at: 3514 3514 Size: 6631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4822 4856 1903 Size: 34 2885 34 3678 Fragments arranged by size: 3678 2885 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4523 5733 4523 Size: 1210 5421 BclI T'GATC_A Cuts at: 1548 1548 Size: 6631 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5242 136 Size: 107 122 71 631 605 337 3233 1525 Fragments arranged by size: 3233 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6626 6626 Size: 6631 BlpI GC'TnA_GC Cuts at: 3144 4048 3144 Size: 904 5727 Bme1580I G_kGCm'C Cuts at: 1790 2850 4692 5848 1790 Size: 1060 1842 1156 2573 Fragments arranged by size: 2573 1842 1156 1060 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6631 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4362 5320 442 Size: 416 53 83 399 24 2945 958 1753 Fragments arranged by size: 2945 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6631 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2898 3336 5291 1239 Size: 1659 438 1955 2579 Fragments arranged by size: 2579 1955 1659 438 Bpu10I CC'TnA_GC Cuts at: 3514 3514 Size: 6631 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3203 4756 5621 5919 3203 Size: 1553 865 298 3915 Fragments arranged by size: 3915 1553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5294 882 Size: 617 3795 2219 Fragments arranged by size: 3795 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4422 493 Size: 711 3218 2702 Fragments arranged by size: 3218 2702 711 BsaBI GATnn'nnATC Cuts at: 2244 6625 2244 Size: 4381 2250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4438 4820 5455 275 Size: 53 83 186 936 2905 382 635 1451 Fragments arranged by size: 2905 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2965 5064 5462 5736 5883 6294 2965 Size: 2099 398 274 147 411 3302 Fragments arranged by size: 3302 2099 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2112 2142 3662 3692 1178 Size: 30 165 30 709 30 1520 30 4117 Fragments arranged by size: 4117 1520 709 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2166 3810 1186 Size: 980 1644 4007 Fragments arranged by size: 4007 1644 980 BseYI C'CCAG_C Cuts at: 5834 6330 5834 Size: 496 6135 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2439 1163 Size: 573 703 5355 Fragments arranged by size: 5355 703 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4081 4842 5870 1540 Size: 275 186 2080 761 1028 2301 Fragments arranged by size: 2301 2080 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2850 3055 4692 4777 5848 2850 Size: 205 1637 85 1071 3633 Fragments arranged by size: 3633 1637 1071 205 85 BsmI GAATG_Cn' Cuts at: 4142 4235 4142 Size: 93 6538 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2850 3055 3950 4692 4777 5848 1391 Size: 399 1060 205 895 742 85 1071 2174 Fragments arranged by size: 2174 1071 1060 895 742 399 205 85 BspEI T'CCGG_A Cuts at: 6294 6294 Size: 6631 BspHI T'CATG_A Cuts at: 3573 4520 6250 3573 Size: 947 1730 3954 Fragments arranged by size: 3954 1730 947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3986 844 Size: 3142 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4518 1394 Size: 429 2695 3507 Fragments arranged by size: 3507 2695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3739 5126 5300 65 Size: 1183 2491 1387 174 1396 Fragments arranged by size: 2491 1396 1387 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5275 5462 1700 Size: 110 3465 187 2869 Fragments arranged by size: 3465 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6631 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6631 BssSI C'ACGA_G Cuts at: 3567 4691 5703 3567 Size: 1124 1012 4495 Fragments arranged by size: 4495 1124 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2417 794 Size: 1623 5008 BstBI TT'CG_AA Cuts at: 2323 5435 2323 Size: 3112 3519 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6631 BstYI r'GATC_y Cuts at: 1972 2245 2317 3961 4328 4713 4730 6182 6268 Size: 273 72 1644 367 385 17 1452 86 Cuts at: 6268 6280 6626 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1644 1452 385 367 346 273 86 72 17 12 BstZ17I GTA'TAC Cuts at: 2272 2272 Size: 6631 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5140 Fragments arranged by size: 5140 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5892 Fragments arranged by size: 5892 683 56 BtsI GCAGTG_nn' Cuts at: 2676 3222 3880 4147 4941 4961 2676 Size: 546 658 267 794 20 4346 Fragments arranged by size: 4346 794 658 546 267 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6631 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6596 DraI TTT'AAA Cuts at: 2352 2871 3935 2352 Size: 519 1064 5048 Fragments arranged by size: 5048 1064 519 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5638 809 Size: 507 4322 1802 Fragments arranged by size: 4322 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2158 2749 3522 Size: 54 1425 50 275 18 328 591 773 Cuts at: 3522 4078 4967 6596 8 Size: 556 889 1629 43 Fragments arranged by size: 1629 1425 889 773 591 556 328 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4078 1537 Size: 275 2266 4090 Fragments arranged by size: 4090 2266 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2600 3321 4561 1353 Size: 1247 721 1240 3423 Fragments arranged by size: 3423 1247 1240 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5218 5592 5738 387 Size: 1064 138 3629 374 146 1280 Fragments arranged by size: 3629 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2880 2898 3336 4694 5291 6078 Size: 393 678 570 18 438 1358 597 787 Cuts at: 6078 6346 1239 Size: 268 1524 Fragments arranged by size: 1524 1358 787 678 597 570 438 393 268 18 EcoNI CCTnn'n_nnAGG Cuts at: 3342 3342 Size: 6631 EcoO109I rG'GnC_Cy Cuts at: 2769 2769 Size: 6631 EcoRI G'AATT_C Cuts at: 3940 4318 3940 Size: 378 6253 EcoRV GAT'ATC Cuts at: 2545 2545 Size: 6631 FspI TGC'GCA Cuts at: 5137 5137 Size: 6631 HaeII r_GCGC'y Cuts at: 1678 5778 1678 Size: 4100 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 3314 3346 3662 Size: 32 135 32 584 32 1126 32 316 Cuts at: 3662 3694 5272 5304 5346 5378 1373 Size: 32 1578 32 42 32 2626 Fragments arranged by size: 2626 1578 1126 584 316 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2587 2788 3829 3975 4221 669 Size: 988 281 649 201 1041 146 246 3079 Fragments arranged by size: 3079 1041 988 649 281 246 201 146 HindIII A'AGCT_T Cuts at: 748 3438 748 Size: 2690 3941 HpaI GTT'AAC Cuts at: 4221 4221 Size: 6631 KpnI G_GTAC'C Cuts at: 3956 3956 Size: 6631 MfeI C'AATT_G Cuts at: 4230 4230 Size: 6631 MluI A'CGCG_T Cuts at: 6507 6507 Size: 6631 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5745 5929 1305 Size: 4440 184 2007 Fragments arranged by size: 4440 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2160 2751 10 Size: 54 1425 343 328 591 3890 Fragments arranged by size: 3890 1425 591 343 328 54 MslI CAynn'nnrTG Cuts at: 518 1166 2558 3122 3213 4589 4948 5107 518 Size: 648 1392 564 91 1376 359 159 2042 Fragments arranged by size: 2042 1392 1376 648 564 359 159 91 MspA1I CmG'CkG Cuts at: 1606 2918 3158 4047 4724 5872 6117 1606 Size: 1312 240 889 677 1148 245 2120 Fragments arranged by size: 2120 1312 1148 889 677 245 240 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5140 Fragments arranged by size: 5140 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6631 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6631 NotI GC'GGCC_GC Cuts at: 4078 4078 Size: 6631 NspI r_CATG'y Cuts at: 1132 1229 3989 5534 1132 Size: 97 2760 1545 2229 Fragments arranged by size: 2760 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6631 PciI A'CATG_T Cuts at: 5530 5530 Size: 6631 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6631 PmeI GTTT'AAAC Cuts at: 3935 3935 Size: 6631 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4700 4732 6213 6245 4700 Size: 32 1481 32 5086 Fragments arranged by size: 5086 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6631 PsiI TTA'TAA Cuts at: 4201 4201 Size: 6631 PstI C_TGCA'G Cuts at: 830 2269 3875 3983 830 Size: 1439 1606 108 3478 Fragments arranged by size: 3478 1606 1439 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6631 PvuII CAG'CTG Cuts at: 2918 3158 2918 Size: 240 6391 SalI G'TCGA_C Cuts at: 1936 3973 1936 Size: 2037 4594 SbfI CC_TGCA'GG Cuts at: 3983 3983 Size: 6631 ScaI AGT'ACT Cuts at: 4879 6591 4879 Size: 1712 4919 SexAI A'CCwGG_T Cuts at: 3615 3615 Size: 6631 SfcI C'TryA_G Cuts at: 826 1045 2265 2580 3700 3871 3979 5114 5795 Size: 219 1220 315 1120 171 108 1135 681 Cuts at: 5795 5986 826 Size: 191 1471 Fragments arranged by size: 1471 1220 1135 1120 681 315 219 191 171 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6631 SmaI CCC'GGG Cuts at: 1872 3958 1872 Size: 2086 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3182 4735 5636 5898 820 Size: 197 926 1239 1553 901 262 1553 Fragments arranged by size: 1553 1553 1239 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4422 493 Size: 3929 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6631 SphI G_CATG'C Cuts at: 1132 3989 1132 Size: 2857 3774 SspI AAT'ATT Cuts at: 5 52 2299 4555 5 Size: 47 2247 2256 2081 Fragments arranged by size: 2256 2247 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6631 StyI C'CwwG_G Cuts at: 513 1065 2004 2436 4070 513 Size: 552 939 432 1634 3074 Fragments arranged by size: 3074 1634 939 552 432 SwaI ATTT'AAAT Cuts at: 2352 2352 Size: 6631 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2606 4658 4675 4828 5013 5458 325 Size: 1255 1026 2052 17 153 185 445 1498 Fragments arranged by size: 2052 1498 1255 1026 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2854 4877 6589 Size: 274 80 33 51 1443 877 2023 1712 Cuts at: 6589 96 Size: 138 Fragments arranged by size: 2023 1712 1443 877 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6591 6623 6591 Size: 32 6599 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6631 XbaI T'CTAG_A Cuts at: 3319 3967 3319 Size: 648 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3550 3784 3550 Size: 234 6397 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6631 XmaI C'CCGG_G Cuts at: 1870 3956 1870 Size: 2086 4545 XmnI GAAnn'nnTTC Cuts at: 3024 4760 3024 Size: 1736 4895 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4439 276 Size: 53 83 186 936 2905 2468 Fragments arranged by size: 2905 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI