Restriction map for
pFN21AA0840
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AlwI AocI
ApaI ApoI AscI AseI AsnI
AspI AvrII BamHI BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BlnI BsaHI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI EarI
Eco81I EcoNI EcoRI EcoT22I FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI Ksp632I MaeI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tsp509I Tth111I XbaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 1058 GGTACC G^GTACC
AflIII 548 ACGTGT A^C[GA][CT]GT
AhdI 647 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 262 GTGCAC G^TGCAC
Aor51HI 1436 AGCGCT AGC^GCT
ApaLI 262 GTGCAC G^TGCAC
Asp700I 1275 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1058 GGTACC G^GTACC
AspEI 647 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 143 TGCGCA TGC^GCA
BanI 1058 GGTACC G^G[CT][GA]CC
BanII 213 GGGCTC G[GA]GC[CT]^C
BbsI 124 GAAGAC GAAGAC(2/6)
BciVI 1243 GTATCC GTATCC(6/5)
BglI 473 GCCTGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 371 AGATCT A^GATCT
BpuAI 124 GAAGAC GAAGAC(2/6)
BsaAI 1108 TACGTG [CT]AC^GT[GA]
BsaBI 813 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 225 CTCCTC GAGGAG(10/8)
BsiEI 1080 CGGCCG CG[GA][CT]^CG
BsmBI 247 GAGACG CGTCTC(1/5)
Bsp1407I 611 TGTACA T^GTACA
BsrBI 295 CCGCTC CCGCTC(-3/-3)
BsrGI 611 TGTACA T^GTACA
BssHII 410 GCGCGC G^CGCGC
BstEII 1086 GGTCACC G^GT[ACGT]ACC
BstPI 1086 GGTCACC G^GT[ACGT]ACC
BstXI 488 CCATCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 371 AGATCT [GA]^GATC[CT]
DraIII 1174 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1387 GACTGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1080 CGGCCG C^GGCCG
Eam1105I 647 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1080 CGGCCG C^GGCCG
Eco47III 1436 AGCGCT AGC^GCT
Eco52I 1080 CGGCCG C^GGCCG
EcoO65I 1086 GGTCACC G^GT[ACGT]ACC
EcoRV 1222 GATATC GAT^ATC
FspI 143 TGCGCA TGC^GCA
HaeII 1436 AGCGCT [GA]GCGC^[CT]
KpnI 1058 GGTACC GGTAC^C
KspI 451 CCGCGG CCGC^GG
MamI 813 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 371 AGATCT [GA]^GATC[CT]
MseI 785 TTAA T^TAA
NspI 140 GCATGC [GA]CATG^[CT]
PaeR7I 48 CTCGAG C^TCGAG
PpuMI 446 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 397 CAGCTG CAG^CTG
SacII 451 CCGCGG CCGC^GG
ScaI 1118 AGTACT AGT^ACT
SexAI 953 ACCTGGT A^CC[AT]GGT
SmaI 770 CCCGGG CCC^GGG
SphI 140 GCATGC GCATG^C
SspBI 611 TGTACA T^GTACA
TfiI 186 GAATC G^A[AT]TC
Tru9I 785 TTAA T^TAA
XcmI 818 CCATCCGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 48 CTCGAG C^TCGAG
XhoII 371 AGATCT [GA]^GATC[CT]
XmaCI 770 CCCGGG C^CCGGG
XmaI 770 CCCGGG C^CCGGG
XmnI 1275 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AvaI 48 CTCGAG C^[CT]CG[GA]G
770 CCCGGG
BalI 254 TGGCCA TGG^CCA
1112 TGGCCA
BlpI 984 GCTGAGC GC^T[ACGT]AGC
1296 GCTCAGC
Bpu1102I 984 GCTGAGC GC^T[ACGT]AGC
1296 GCTCAGC
BsaI 484 GAGACC GGTCTC(1/5)
1395 GGTCTC
BsoBI 48 CTCGAG C^[CT]CG[GA]G
770 CCCGGG
BspMI 754 ACCTGC ACCTGC(4/8)
911 ACCTGC
BsrFI 344 ACCGGC [GA]^CCGG[CT]
1324 ACCGGC
BsrI 648 ACTGG ACTGG(1/-1)
624 CCAGT
BstAPI 524 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1433 GCAAGCGCTGC
CelII 984 GCTGAGC GC^T[ACGT]AGC
1296 GCTCAGC
Cfr10I 344 ACCGGC [GA]^CCGG[CT]
1324 ACCGGC
DraII 446 GGGACCC [GA]G^G[ACGT]CC[CT]
1406 AGGCCCT
EcoO109I 446 GGGACCC [GA]G^G[ACGT]CC[CT]
1406 AGGCCCT
EcoT14I 257 CCATGG C^C[AT][AT]GG
362 CCATGG
MluNI 254 TGGCCA TGG^CCA
1112 TGGCCA
MscI 254 TGGCCA TGG^CCA
1112 TGGCCA
NcoI 257 CCATGG C^CATGG
362 CCATGG
PflMI 357 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1173 CCACGGTGTGG
PstI 585 CTGCAG CTGCA^G
1122 CTGCAG
SfcI 585 CTGCAG C^T[GA][CT]AG
1122 CTGCAG
StyI 257 CCATGG C^C[AT][AT]GG
362 CCATGG
TaqI 49 TCGA T^CGA
1448 TCGA
TthHB8I 49 TCGA T^CGA
1448 TCGA
Van91I 357 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1173 CCACGGTGTGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
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AccII AciI AfaI AluI AlwNI
AspHI AvaII BbvI BcnI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StuI TseI Tsp45I TspRI