(Circular) (Six-base) MAPSORT of: pFN21AA0840.seq Check: 5710 from: 1 to: 6178 pFN21AA0840 6178 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3988 278 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3521 1937 Size: 1584 4594 Acc65I G'GTAC_C Cuts at: 3063 3499 3063 Size: 436 5742 AclI AA'CG_TT Cuts at: 1563 4305 4678 1563 Size: 2742 373 3063 Fragments arranged by size: 3063 2742 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4241 5625 5893 1632 Size: 2609 1384 268 1917 Fragments arranged by size: 2609 1917 1384 268 AfeI AGC'GCT Cuts at: 3443 3443 Size: 6178 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5981 AflIII A'CryG_T Cuts at: 1203 2553 5077 6054 1203 Size: 1350 2524 977 1327 Fragments arranged by size: 2524 1350 1327 977 AgeI A'CCGG_T Cuts at: 5009 5009 Size: 6178 AhdI GACnn_n'nnGTC Cuts at: 2657 4907 2657 Size: 2250 3928 AleI CACnn'nnGTG Cuts at: 1166 3098 1166 Size: 1932 4246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3152 1184 Size: 1968 4210 AlwNI CAG_nnn'CTG Cuts at: 2535 2589 3003 3379 3921 5493 2535 Size: 54 414 376 542 1572 3220 Fragments arranged by size: 3220 1572 542 414 376 54 ApaLI G'TGCA_C Cuts at: 2267 4235 5391 2267 Size: 1968 1156 3054 Fragments arranged by size: 3054 1968 1156 ApoI r'AATT_y Cuts at: 1493 3487 3713 3865 5974 1493 Size: 1994 226 152 2109 1697 Fragments arranged by size: 2109 1994 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4732 160 Size: 872 3700 1606 Fragments arranged by size: 3700 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 AvaI C'yCGr_G Cuts at: 1870 1943 2053 2775 3503 1870 Size: 73 110 722 728 4545 Fragments arranged by size: 4545 728 722 110 73 BamHI G'GATC_C Cuts at: 3508 3875 3508 Size: 367 5811 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3063 3499 4954 618 Size: 325 217 627 1276 436 1455 1842 Fragments arranged by size: 1842 1455 1276 627 436 325 217 BanII G_rGCy'C Cuts at: 1391 2222 3497 1391 Size: 831 1275 4072 Fragments arranged by size: 4072 1275 831 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2136 928 Size: 971 237 4970 Fragments arranged by size: 4970 971 237 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4369 4403 1903 Size: 34 2432 34 3678 Fragments arranged by size: 3678 2432 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3259 4070 5280 3259 Size: 811 1210 4157 Fragments arranged by size: 4157 1210 811 BclI T'GATC_A Cuts at: 1548 1548 Size: 6178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2484 4789 136 Size: 107 122 71 631 605 812 2305 1525 Fragments arranged by size: 2305 1525 812 631 605 122 107 71 BglII A'GATC_T Cuts at: 2376 6173 2376 Size: 3797 2381 BlpI GC'TnA_GC Cuts at: 2990 3302 3595 2990 Size: 312 293 5573 Fragments arranged by size: 5573 312 293 Bme1580I G_kGCm'C Cuts at: 1790 2271 4239 5395 1790 Size: 481 1968 1156 2573 Fragments arranged by size: 2573 1968 1156 481 BmgBI CAC'GTC Cuts at: 1318 2071 3100 1318 Size: 753 1029 4396 Fragments arranged by size: 4396 1029 753 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2623 3909 4867 Size: 416 53 83 399 24 1206 1286 958 Cuts at: 4867 442 Size: 1753 Fragments arranged by size: 1753 1286 1206 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2302 2485 2744 2879 3068 3284 3356 4838 Size: 1063 183 259 135 189 216 72 1482 Cuts at: 4838 1239 Size: 2579 Fragments arranged by size: 2579 1482 1063 259 216 189 183 135 72 Bpu10I CC'TnA_GC Cuts at: 2141 3068 2141 Size: 927 5251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 3278 3293 4303 5168 5466 2498 Size: 780 15 1010 865 298 3210 Fragments arranged by size: 3210 1010 865 780 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2483 3406 4841 882 Size: 617 984 923 1435 2219 Fragments arranged by size: 2219 1435 984 923 617 BsaAI yAC'GTr Cuts at: 493 1204 3115 3969 493 Size: 711 1911 854 2702 Fragments arranged by size: 2702 1911 854 711 BsaBI GATnn'nnATC Cuts at: 2822 6172 2822 Size: 3350 2828 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3985 4367 5002 275 Size: 53 83 186 936 2452 382 635 1451 Fragments arranged by size: 2452 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4611 5009 5283 5430 5841 4611 Size: 398 274 147 411 4948 Fragments arranged by size: 4948 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2178 2208 2663 2693 2960 Size: 30 165 30 775 30 455 30 267 Cuts at: 2960 2990 1178 Size: 30 4366 Fragments arranged by size: 4366 775 455 267 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2221 1186 Size: 1035 5143 BseYI C'CCAG_C Cuts at: 2165 2170 5381 5877 2165 Size: 5 3211 496 2466 Fragments arranged by size: 3211 2466 496 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2393 2858 2878 3190 1163 Size: 573 657 465 20 312 4151 Fragments arranged by size: 4151 657 573 465 312 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3628 4389 5417 1540 Size: 275 186 1087 540 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 540 275 186 BsiHKAI G_wGCw'C Cuts at: 2162 2271 2730 3459 4239 4324 5395 2162 Size: 109 459 729 780 85 1071 2945 Fragments arranged by size: 2945 1071 780 729 459 109 85 BsmI GAATG_Cn' Cuts at: 3689 3782 3689 Size: 93 6085 BsmBI CGTCTCn'nnnn_ Cuts at: 2246 2246 Size: 6178 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2222 2271 2730 3459 3497 4239 Size: 399 372 60 49 459 729 38 742 Cuts at: 4239 4324 5395 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 729 459 399 372 85 60 49 38 BspEI T'CCGG_A Cuts at: 5841 5841 Size: 6178 BspHI T'CATG_A Cuts at: 4067 5797 4067 Size: 1730 4448 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2768 2925 3533 844 Size: 1924 157 608 3489 Fragments arranged by size: 3489 1924 608 157 BsrBI CCG'CTC Cuts at: 1394 1823 2302 4065 1394 Size: 429 479 1763 3507 Fragments arranged by size: 3507 1763 479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4673 4847 65 Size: 1183 3425 174 1396 Fragments arranged by size: 3425 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2349 3329 4822 5009 1700 Size: 110 539 980 1493 187 2869 Fragments arranged by size: 2869 1493 980 539 187 110 BsrGI T'GTAC_A Cuts at: 96 2616 96 Size: 2520 3658 BssHII G'CGCG_C Cuts at: 1925 2415 1925 Size: 490 5688 BssSI C'ACGA_G Cuts at: 4238 5250 4238 Size: 1012 5166 BstAPI GCAn_nnn'nTGC Cuts at: 794 2535 3444 794 Size: 1741 909 3528 Fragments arranged by size: 3528 1741 909 BstBI TT'CG_AA Cuts at: 4982 4982 Size: 6178 BstEII G'GTnAC_C Cuts at: 1184 3091 1184 Size: 1907 4271 BstXI CCAn_nnnn'nTGG Cuts at: 2500 2500 Size: 6178 BstYI r'GATC_y Cuts at: 1972 2376 3508 3875 4260 4277 5729 5815 5827 Size: 404 1132 367 385 17 1452 86 12 Cuts at: 5827 6173 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1132 404 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2262 2367 2456 3178 3405 513 Size: 552 939 258 105 89 722 227 3286 Fragments arranged by size: 3286 939 722 552 258 227 105 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2608 2866 3058 487 Size: 683 56 1382 258 192 3607 Fragments arranged by size: 3607 1382 683 258 192 56 BtsI GCAGTG_nn' Cuts at: 2041 2890 3079 3433 3694 4488 4508 2041 Size: 849 189 354 261 794 20 3711 Fragments arranged by size: 3711 849 794 354 261 189 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6143 DraI TTT'AAA Cuts at: 3482 3482 Size: 6178 DraIII CAC_nnn'GTG Cuts at: 3184 3184 Size: 6178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3398 5185 809 Size: 507 2082 1787 1802 Fragments arranged by size: 2082 1802 1787 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 3085 3117 Size: 54 1425 50 275 18 429 826 32 Cuts at: 3117 3331 3351 3625 4514 6143 8 Size: 214 20 274 889 1629 43 Fragments arranged by size: 1629 1425 889 826 429 275 274 214 54 50 43 32 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 3625 1537 Size: 275 1273 540 4090 Fragments arranged by size: 4090 1273 540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4108 1353 Size: 2755 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2465 2921 4765 5139 5285 387 Size: 1064 138 876 456 1844 374 146 1280 Fragments arranged by size: 1844 1280 1064 876 456 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2302 2485 2744 2879 3068 3284 3356 Size: 393 670 183 259 135 189 216 72 Cuts at: 3356 4241 4838 5625 5893 1239 Size: 885 597 787 268 1524 Fragments arranged by size: 1524 885 787 670 597 393 268 259 216 189 183 135 72 EcoO109I rG'GnC_Cy Cuts at: 2452 3412 2452 Size: 960 5218 EcoRI G'AATT_C Cuts at: 3487 3865 3487 Size: 378 5800 EcoRV GAT'ATC Cuts at: 3229 3229 Size: 6178 FspI TGC'GCA Cuts at: 2150 4684 2150 Size: 2534 3644 FspAI rTGC'GCAy Cuts at: 2150 2150 Size: 6178 HaeII r_GCGC'y Cuts at: 1678 3445 5325 1678 Size: 1767 1880 2531 Fragments arranged by size: 2531 1880 1767 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2643 2675 2690 2722 4819 Size: 32 135 32 1071 32 15 32 2097 Cuts at: 4819 4851 4893 4925 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2097 1071 135 42 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 3522 3768 669 Size: 988 281 1584 246 3079 Fragments arranged by size: 3079 1584 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6178 HpaI GTT'AAC Cuts at: 3768 3768 Size: 6178 KpnI G_GTAC'C Cuts at: 3067 3503 3067 Size: 436 5742 MfeI C'AATT_G Cuts at: 3777 3777 Size: 6178 MluI A'CGCG_T Cuts at: 6054 6054 Size: 6178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2160 2706 3276 5292 5476 1305 Size: 855 546 570 2016 184 2007 Fragments arranged by size: 2016 2007 855 570 546 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 3119 10 Size: 54 1425 343 429 858 3069 Fragments arranged by size: 3069 1425 858 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2498 2733 3098 4136 4495 4654 518 Size: 648 1332 235 365 1038 359 159 2042 Fragments arranged by size: 2042 1332 1038 648 365 359 235 159 MspA1I CmG'CkG Cuts at: 1606 2404 2412 2433 2458 2925 3594 4271 5419 Size: 798 8 21 25 467 669 677 1148 Cuts at: 5419 5664 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 798 677 669 467 245 25 21 8 NcoI C'CATG_G Cuts at: 513 1065 2004 2262 2367 513 Size: 552 939 258 105 4324 Fragments arranged by size: 4324 939 552 258 105 NdeI CA'TA_TG Cuts at: 387 387 Size: 6178 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6178 NotI GC'GGCC_GC Cuts at: 3625 3625 Size: 6178 NspI r_CATG'y Cuts at: 1132 1229 2149 3536 5081 1132 Size: 97 920 1387 1545 2229 Fragments arranged by size: 2229 1545 1387 920 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6178 PciI A'CATG_T Cuts at: 5077 5077 Size: 6178 PflMI CCAn_nnn'nTGG Cuts at: 1108 2368 3184 1108 Size: 1260 816 4102 Fragments arranged by size: 4102 1260 816 PfoI T'CCnGG_A Cuts at: 2355 3387 2355 Size: 1032 5146 PmeI GTTT'AAAC Cuts at: 3482 3482 Size: 6178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4247 4279 5760 5792 4247 Size: 32 1481 32 4633 Fragments arranged by size: 4633 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2452 Size: 6178 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6178 PsiI TTA'TAA Cuts at: 3748 3748 Size: 6178 PspXI vC'TCGA_Gb Cuts at: 2053 2053 Size: 6178 PstI C_TGCA'G Cuts at: 830 2594 3131 3530 830 Size: 1764 537 399 3478 Fragments arranged by size: 3478 1764 537 399 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6178 PvuII CAG'CTG Cuts at: 2404 2404 Size: 6178 SacII CC_GC'GG Cuts at: 2459 2459 Size: 6178 SalI G'TCGA_C Cuts at: 1936 3520 1936 Size: 1584 4594 SanDI GG'GwC_CC Cuts at: 2452 2452 Size: 6178 SbfI CC_TGCA'GG Cuts at: 3530 3530 Size: 6178 ScaI AGT'ACT Cuts at: 3125 4426 6138 3125 Size: 1301 1712 3165 Fragments arranged by size: 3165 1712 1301 SexAI A'CCwGG_T Cuts at: 2958 2958 Size: 6178 SfcI C'TryA_G Cuts at: 826 1045 2590 3127 3526 4661 5342 5533 826 Size: 219 1545 537 399 1135 681 191 1471 Fragments arranged by size: 1545 1471 1135 681 537 399 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6178 SmaI CCC'GGG Cuts at: 1872 2777 3505 1872 Size: 905 728 4545 Fragments arranged by size: 4545 905 728 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 2513 3293 3308 4282 5183 Size: 197 926 110 460 780 15 974 901 Cuts at: 5183 5445 820 Size: 262 1553 Fragments arranged by size: 1553 974 926 901 780 460 262 197 110 15 SnaBI TAC'GTA Cuts at: 493 3969 493 Size: 3476 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6178 SphI G_CATG'C Cuts at: 1132 2149 3536 1132 Size: 1017 1387 3774 Fragments arranged by size: 3774 1387 1017 SspI AAT'ATT Cuts at: 5 52 4102 5 Size: 47 4050 2081 Fragments arranged by size: 4050 2081 47 StuI AGG'CCT Cuts at: 1517 2783 2871 2949 1517 Size: 1266 88 78 4746 Fragments arranged by size: 4746 1266 88 78 StyI C'CwwG_G Cuts at: 513 1065 2004 2262 2367 3617 513 Size: 552 939 258 105 1250 3074 Fragments arranged by size: 3074 1250 939 552 258 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2622 2921 2954 4205 4222 4375 4560 Size: 1255 1042 299 33 1251 17 153 185 Cuts at: 4560 5005 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1251 1042 445 299 185 153 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2081 2616 3123 Size: 274 80 33 51 1443 104 535 507 Cuts at: 3123 4424 6136 96 Size: 1301 1712 138 Fragments arranged by size: 1712 1443 1301 535 507 274 138 104 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2178 2210 6138 6170 2178 Size: 32 3928 32 2186 Fragments arranged by size: 3928 2186 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6178 XbaI T'CTAG_A Cuts at: 3514 3514 Size: 6178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2830 2830 Size: 6178 XhoI C'TCGA_G Cuts at: 1943 2053 1943 Size: 110 6068 XmaI C'CCGG_G Cuts at: 1870 2775 3503 1870 Size: 905 728 4545 Fragments arranged by size: 4545 905 728 XmnI GAAnn'nnTTC Cuts at: 3284 4307 3284 Size: 1023 5155 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3986 276 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI