Restriction map for
pFN21AA0840
(Linear) (Six-base) MAPSORT of: KIAA0840.seq Check: 770 from: 1 to: 1473
KIAA0840.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
Acc65I G'GTAC_C
Cuts at: 0 1058 1473
Size: 1058 415
AfeI AGC'GCT
Cuts at: 0 1438 1473
Size: 1438 35
AflIII A'CryG_T
Cuts at: 0 548 1473
Size: 548 925
AhdI GACnn_n'nnGTC
Cuts at: 0 652 1473
Size: 652 821
AleI CACnn'nnGTG
Cuts at: 0 1093 1473
Size: 1093 380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1147 1473
Size: 1147 326
AlwNI CAG_nnn'CTG
Cuts at: 0 530 584 998 1374 1473
Size: 530 54 414 376 99
Fragments arranged by size:
530 414 376 99 54
ApaLI G'TGCA_C
Cuts at: 0 262 1473
Size: 262 1211
AvaI C'yCGr_G
Cuts at: 0 48 770 1473
Size: 48 722 703
Fragments arranged by size:
722 703 48
BanI G'GyrC_C
Cuts at: 0 1058 1473
Size: 1058 415
BanII G_rGCy'C
Cuts at: 0 217 1473
Size: 217 1256
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 1473
Size: 131 1342
BciVI GTATCCnnnnn_n'
Cuts at: 0 1254 1473
Size: 1254 219
BglI GCCn_nnn'nGGC
Cuts at: 0 479 1473
Size: 479 994
BglII A'GATC_T
Cuts at: 0 371 1473
Size: 371 1102
BlpI GC'TnA_GC
Cuts at: 0 985 1297 1473
Size: 985 312 176
Fragments arranged by size:
985 312 176
Bme1580I G_kGCm'C
Cuts at: 0 266 1473
Size: 266 1207
BmgBI CAC'GTC
Cuts at: 0 66 1095 1473
Size: 66 1029 378
Fragments arranged by size:
1029 378 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 618 1473
Size: 618 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 480 739 874 1063 1279 1351 1473
Size: 297 183 259 135 189 216 72 122
Fragments arranged by size:
297 259 216 189 183 135 122 72
Bpu10I CC'TnA_GC
Cuts at: 0 136 1063 1473
Size: 136 927 410
Fragments arranged by size:
927 410 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1273 1288 1473
Size: 493 780 15 185
Fragments arranged by size:
780 493 185 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 478 1401 1473
Size: 478 923 72
Fragments arranged by size:
923 478 72
BsaAI yAC'GTr
Cuts at: 0 1110 1473
Size: 1110 363
BsaBI GATnn'nnATC
Cuts at: 0 817 1473
Size: 817 656
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 173 203 658 688 955 985 1473
Size: 173 30 455 30 267 30 488
Fragments arranged by size:
488 455 267 173 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 1473
Size: 216 1257
BseYI C'CCAG_C
Cuts at: 0 160 165 1473
Size: 160 5 1308
Fragments arranged by size:
1308 160 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 853 873 1185 1473
Size: 388 465 20 312 288
Fragments arranged by size:
465 388 312 288 20
BsiEI CG_ry'CG
Cuts at: 0 1083 1473
Size: 1083 390
BsiHKAI G_wGCw'C
Cuts at: 0 157 266 725 1454 1473
Size: 157 109 459 729 19
Fragments arranged by size:
729 459 157 109 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 241 1473
Size: 241 1232
Bsp1286I G_dGCh'C
Cuts at: 0 157 217 266 725 1454 1473
Size: 157 60 49 459 729 19
Fragments arranged by size:
729 459 157 60 49 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 763 920 1473
Size: 763 157 553
Fragments arranged by size:
763 553 157
BsrBI CCG'CTC
Cuts at: 0 297 1473
Size: 297 1176
BsrFI r'CCGG_y
Cuts at: 0 344 1324 1473
Size: 344 980 149
Fragments arranged by size:
980 344 149
BsrGI T'GTAC_A
Cuts at: 0 611 1473
Size: 611 862
BssHII G'CGCG_C
Cuts at: 0 410 1473
Size: 410 1063
BstAPI GCAn_nnn'nTGC
Cuts at: 0 530 1439 1473
Size: 530 909 34
Fragments arranged by size:
909 530 34
BstEII G'GTnAC_C
Cuts at: 0 1086 1473
Size: 1086 387
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 1473
Size: 495 978
BstYI r'GATC_y
Cuts at: 0 371 1473
Size: 371 1102
BtgI C'CryG_G
Cuts at: 0 257 362 451 1173 1400 1473
Size: 257 105 89 722 227 73
Fragments arranged by size:
722 257 227 105 89 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 603 861 1053 1473
Size: 603 258 192 420
Fragments arranged by size:
603 420 258 192
BtsI GCAGTG_nn'
Cuts at: 0 36 885 1074 1428 1473
Size: 36 849 189 354 45
Fragments arranged by size:
849 354 189 45 36
DraIII CAC_nnn'GTG
Cuts at: 0 1179 1473
Size: 1179 294
DrdI GACnn_nn'nnGTC
Cuts at: 0 1393 1473
Size: 1393 80
EaeI y'GGCC_r
Cuts at: 0 254 1080 1112 1326 1346 1473
Size: 254 826 32 214 20 127
Fragments arranged by size:
826 254 214 127 32 20
EagI C'GGCC_G
Cuts at: 0 1080 1473
Size: 1080 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 460 916 1473
Size: 460 456 557
Fragments arranged by size:
557 460 456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 480 739 874 1063 1279 1351 1473
Size: 297 183 259 135 189 216 72 122
Fragments arranged by size:
297 259 216 189 183 135 122 72
EcoO109I rG'GnC_Cy
Cuts at: 0 447 1407 1473
Size: 447 960 66
Fragments arranged by size:
960 447 66
EcoRV GAT'ATC
Cuts at: 0 1224 1473
Size: 1224 249
FspI TGC'GCA
Cuts at: 0 145 1473
Size: 145 1328
FspAI rTGC'GCAy
Cuts at: 0 145 1473
Size: 145 1328
HaeII r_GCGC'y
Cuts at: 0 1440 1473
Size: 1440 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 638 670 685 717 1473
Size: 638 32 15 32 756
Fragments arranged by size:
756 638 32 32 15
KpnI G_GTAC'C
Cuts at: 0 1062 1473
Size: 1062 411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 701 1271 1473
Size: 155 546 570 202
Fragments arranged by size:
570 546 202 155
MscI TGG'CCA
Cuts at: 0 256 1114 1473
Size: 256 858 359
Fragments arranged by size:
858 359 256
MslI CAynn'nnrTG
Cuts at: 0 493 728 1093 1473
Size: 493 235 365 380
Fragments arranged by size:
493 380 365 235
MspA1I CmG'CkG
Cuts at: 0 399 407 428 453 920 1473
Size: 399 8 21 25 467 553
Fragments arranged by size:
553 467 399 25 21 8
NcoI C'CATG_G
Cuts at: 0 257 362 1473
Size: 257 105 1111
Fragments arranged by size:
1111 257 105
NspI r_CATG'y
Cuts at: 0 144 1473
Size: 144 1329
PflMI CCAn_nnn'nTGG
Cuts at: 0 363 1179 1473
Size: 363 816 294
Fragments arranged by size:
816 363 294
PfoI T'CCnGG_A
Cuts at: 0 350 1382 1473
Size: 350 1032 91
Fragments arranged by size:
1032 350 91
PpuMI rG'GwC_Cy
Cuts at: 0 447 1473
Size: 447 1026
PspXI vC'TCGA_Gb
Cuts at: 0 48 1473
Size: 48 1425
PstI C_TGCA'G
Cuts at: 0 589 1126 1473
Size: 589 537 347
Fragments arranged by size:
589 537 347
PvuII CAG'CTG
Cuts at: 0 399 1473
Size: 399 1074
SacII CC_GC'GG
Cuts at: 0 454 1473
Size: 454 1019
SanDI GG'GwC_CC
Cuts at: 0 447 1473
Size: 447 1026
ScaI AGT'ACT
Cuts at: 0 1120 1473
Size: 1120 353
SexAI A'CCwGG_T
Cuts at: 0 953 1473
Size: 953 520
SfcI C'TryA_G
Cuts at: 0 585 1122 1473
Size: 585 537 351
Fragments arranged by size:
585 537 351
SmaI CCC'GGG
Cuts at: 0 772 1473
Size: 772 701
SmlI C'TyrA_G
Cuts at: 0 48 508 1288 1303 1473
Size: 48 460 780 15 170
Fragments arranged by size:
780 460 170 48 15
SphI G_CATG'C
Cuts at: 0 144 1473
Size: 144 1329
StuI AGG'CCT
Cuts at: 0 778 866 944 1473
Size: 778 88 78 529
Fragments arranged by size:
778 529 88 78
StyI C'CwwG_G
Cuts at: 0 257 362 1473
Size: 257 105 1111
Fragments arranged by size:
1111 257 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 617 916 949 1473
Size: 617 299 33 524
Fragments arranged by size:
617 524 299 33
TatI w'GTAC_w
Cuts at: 0 76 611 1118 1473
Size: 76 535 507 355
Fragments arranged by size:
535 507 355 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 173 205 1473
Size: 173 32 1268
Fragments arranged by size:
1268 173 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 825 1473
Size: 825 648
XhoI C'TCGA_G
Cuts at: 0 48 1473
Size: 48 1425
XmaI C'CCGG_G
Cuts at: 0 770 1473
Size: 770 703
XmnI GAAnn'nnTTC
Cuts at: 0 1279 1473
Size: 1279 194
Enzymes that do cut:
Acc65I AfeI AflIII AhdI AleI AlfI AlwNI ApaLI
AvaI BanI BanII BbsI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EciI Eco57MI EcoO109I EcoRV FspI FspAI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NcoI NspI
PflMI PfoI PpuMI PspXI PstI PvuII SacII SanDI
ScaI SexAI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BclI BfrBI BmtI BplI BsaHI
BsaWI BsiWI BsmI BspEI BspHI BsrDI BssSI BstBI
BstZ17I Bsu36I ClaI CspCI DraI EarI EcoICRI EcoNI
EcoRI FalI FseI HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI RsrII SacI SalI SapI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI ZraI