Restriction map for
pFN21AA0838
(Circular) (Six-base) MAPSORT of: pFN21AA0838.seq Check: 1819 from: 1 to: 6712
pFN21AA0838 6712 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4522 278
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4055 1937
Size: 2118 4594
Acc65I G'GTAC_C
Cuts at: 4033 4033
Size: 6712
AclI AA'CG_TT
Cuts at: 1563 3134 4839 5212 1563
Size: 1571 1705 373 3063
Fragments arranged by size:
3063 1705 1571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2469 2840 3375 4775 6159 6427 1632
Size: 837 371 535 1400 1384 268 1917
Fragments arranged by size:
1917 1400 1384 837 535 371 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6515
AflIII A'CryG_T
Cuts at: 1203 2343 5611 6588 1203
Size: 1140 3268 977 1327
Fragments arranged by size:
3268 1327 1140 977
AgeI A'CCGG_T
Cuts at: 5543 5543
Size: 6712
AhdI GACnn_n'nnGTC
Cuts at: 2677 5441 2677
Size: 2764 3948
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6712
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2503 2535 2503
Size: 32 6680
AlwNI CAG_nnn'CTG
Cuts at: 2251 2407 4455 6027 2251
Size: 156 2048 1572 2936
Fragments arranged by size:
2936 2048 1572 156
ApaI G_GGCC'C
Cuts at: 2147 2147
Size: 6712
ApaLI G'TGCA_C
Cuts at: 4769 5925 4769
Size: 1156 5556
ApoI r'AATT_y
Cuts at: 1493 2477 2768 3065 3166 3546 3812 3915 4021
Size: 984 291 297 101 380 266 103 106
Cuts at: 4021 4247 4399 6508 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 984 380 297 291 266 226
152 106 103 101
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2118 2279 2330 4037 1870
Size: 73 80 95 161 51 1707 4545
Fragments arranged by size:
4545 1707 161 95 80 73 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2826 2859 2826
Size: 33 6679
BamHI G'GATC_C
Cuts at: 4042 4409 4042
Size: 367 6345
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2308 4033 5488 618
Size: 325 217 627 521 1725 1455 1842
Fragments arranged by size:
1842 1725 1455 627 521 325 217
BanII G_rGCy'C
Cuts at: 1391 2147 2205 3353 4031 1391
Size: 756 58 1148 678 4072
Fragments arranged by size:
4072 1148 756 678 58
BbeI G_GCGC'C
Cuts at: 2312 2312
Size: 6712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2846 3428 928
Size: 971 947 582 4212
Fragments arranged by size:
4212 971 947 582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4903 4937 1903
Size: 34 2966 34 3678
Fragments arranged by size:
3678 2966 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4604 5814 4604
Size: 1210 5502
BclI T'GATC_A
Cuts at: 1548 3646 1548
Size: 2098 4614
BfrBI ATG'CAT
Cuts at: 3385 3385
Size: 6712
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2306 5323 136
Size: 107 122 71 631 605 634 3017 1525
Fragments arranged by size:
3017 1525 634 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2238 2596 6707 2238
Size: 358 4111 2243
Fragments arranged by size:
4111 2243 358
BlpI GC'TnA_GC
Cuts at: 4129 4129
Size: 6712
Bme1580I G_kGCm'C
Cuts at: 1790 2089 2147 4773 5929 1790
Size: 299 58 2626 1156 2573
Fragments arranged by size:
2626 2573 1156 299 58
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6712
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4443 5401 442
Size: 416 53 83 399 24 3026 958 1753
Fragments arranged by size:
3026 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6712
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2091 2091
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2751 2850 3033 3471 3714 5372 1239
Size: 1512 99 183 438 243 1658 2579
Fragments arranged by size:
2579 1658 1512 438 243 183 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3933 4837 5702 6000 3933
Size: 904 865 298 4645
Fragments arranged by size:
4645 904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3513 5375 882
Size: 617 2014 1862 2219
Fragments arranged by size:
2219 2014 1862 617
BsaAI yAC'GTr
Cuts at: 493 1204 4503 493
Size: 711 3299 2702
Fragments arranged by size:
3299 2702 711
BsaBI GATnn'nnATC
Cuts at: 6706 6706
Size: 6712
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2113 2309 4519 4901
Size: 53 83 186 936 580 196 2210 382
Cuts at: 4901 5536 275
Size: 635 1451
Fragments arranged by size:
2210 1451 936 635 580 382 196 186
83 53
BsaWI w'CCGG_w
Cuts at: 5145 5543 5817 5964 6375 5145
Size: 398 274 147 411 5482
Fragments arranged by size:
5482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2724 2754 3006 3036 1178
Size: 30 165 30 1321 30 252 30 4854
Fragments arranged by size:
4854 1321 252 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3501 3504 1186
Size: 2315 3 4394
Fragments arranged by size:
4394 2315 3
BseYI C'CCAG_C
Cuts at: 2251 3458 5915 6411 2251
Size: 1207 2457 496 2552
Fragments arranged by size:
2552 2457 1207 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3690 3872 1163
Size: 573 1954 182 4003
Fragments arranged by size:
4003 1954 573 182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2054 2135 2139 4162 4923 5951
Size: 275 186 53 81 4 2023 761 1028
Cuts at: 5951 1540
Size: 2301
Fragments arranged by size:
2301 2023 1028 761 275 186 81 53
4
BsiHKAI G_wGCw'C
Cuts at: 2267 3260 4773 4858 5929 2267
Size: 993 1513 85 1071 3050
Fragments arranged by size:
3050 1513 1071 993 85
BsmI GAATG_Cn'
Cuts at: 2821 3385 3465 4223 4316 2821
Size: 564 80 758 93 5217
Fragments arranged by size:
5217 758 564 93 80
BsmBI CGTCTCn'nnnn_
Cuts at: 2330 2330
Size: 6712
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2147 2205 2267 3260 3353 4031
Size: 399 299 58 58 62 993 93 678
Cuts at: 4031 4773 4858 5929 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 993 742 678 399 299 93
85 62 58 58
BspEI T'CCGG_A
Cuts at: 6375 6375
Size: 6712
BspHI T'CATG_A
Cuts at: 4601 6331 4601
Size: 1730 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 4067 844
Size: 1205 2018 3489
Fragments arranged by size:
3489 2018 1205
BsrBI CCG'CTC
Cuts at: 1394 1823 4599 1394
Size: 429 2776 3507
Fragments arranged by size:
3507 2776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2886 5207 5381 65
Size: 1183 1638 2321 174 1396
Fragments arranged by size:
2321 1638 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2109 2134 2194 5356 5543 1700
Size: 110 250 49 25 60 3162 187 2869
Fragments arranged by size:
3162 2869 250 187 110 60 49 25
BsrGI T'GTAC_A
Cuts at: 96 2801 96
Size: 2705 4007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6712
BssSI C'ACGA_G
Cuts at: 4772 5784 4772
Size: 1012 5700
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6712
BstBI TT'CG_AA
Cuts at: 3364 3712 5516 3364
Size: 348 1804 4560
Fragments arranged by size:
4560 1804 348
BstEII G'GTnAC_C
Cuts at: 1184 2098 1184
Size: 914 5798
BstXI CCAn_nnnn'nTGG
Cuts at: 3292 3490 3292
Size: 198 6514
BstYI r'GATC_y
Cuts at: 1972 2238 2596 4042 4409 4794 4811 6263 6349
Size: 266 358 1446 367 385 17 1452 86
Cuts at: 6349 6361 6707 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1446 385 367 358 346 266
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2380 2380
Size: 6712
BtgI C'CryG_G
Cuts at: 513 1065 2139 2171 3873 513
Size: 552 1074 32 1702 3352
Fragments arranged by size:
3352 1702 1074 552 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5973
Fragments arranged by size:
5973 683 56
BtsI GCAGTG_nn'
Cuts at: 3884 4228 5022 5042 3884
Size: 344 794 20 5554
Fragments arranged by size:
5554 794 344 20
ClaI AT'CG_AT
Cuts at: 1554 2923 1554
Size: 1369 5343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 2605 3913 4016 2605
Size: 1308 103 5301
Fragments arranged by size:
5301 1308 103
DraIII CAC_nnn'GTG
Cuts at: 2106 2106
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5719 809
Size: 507 4403 1802
Fragments arranged by size:
4403 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2132 2136 2192
Size: 54 1425 50 275 18 302 4 56
Cuts at: 2192 2763 4159 5048 6677 8
Size: 571 1396 889 1629 43
Fragments arranged by size:
1629 1425 1396 889 571 302 275 56
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2132 2136 4159 1537
Size: 275 320 4 2023 4090
Fragments arranged by size:
4090 2023 320 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2565 3628 4642 1353
Size: 1212 1063 1014 3423
Fragments arranged by size:
3423 1212 1063 1014
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 5299 5673 5819 387
Size: 1064 138 624 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 624 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 2751 2840 2850 3033 3375 3471
Size: 393 837 282 89 10 183 342 96
Cuts at: 3471 3714 4775 5372 6159 6427 1239
Size: 243 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 837 787 597 393 342 282
268 243 183 96 89 10
EcoO109I rG'GnC_Cy
Cuts at: 2038 2215 2038
Size: 177 6535
EcoRI G'AATT_C
Cuts at: 3546 4021 4399 3546
Size: 475 378 5859
Fragments arranged by size:
5859 475 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3738 3738
Size: 6712
FseI GG_CCGG'CC
Cuts at: 2138 2138
Size: 6712
FspI TGC'GCA
Cuts at: 5218 5218
Size: 6712
HaeII r_GCGC'y
Cuts at: 1678 2312 5859 1678
Size: 634 3547 2531
Fragments arranged by size:
3547 2531 634
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2254 2286 2663 2695 5353
Size: 32 135 32 682 32 377 32 2658
Cuts at: 5353 5385 5427 5459 1373
Size: 32 42 32 2626
Fragments arranged by size:
2658 2626 682 377 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 4056 4302 669
Size: 988 281 699 1419 246 3079
Fragments arranged by size:
3079 1419 988 699 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6712
HpaI GTT'AAC
Cuts at: 4302 4302
Size: 6712
KasI G'GCGC_C
Cuts at: 2308 2308
Size: 6712
KpnI G_GTAC'C
Cuts at: 4037 4037
Size: 6712
MfeI C'AATT_G
Cuts at: 2442 3018 4311 2442
Size: 576 1293 4843
Fragments arranged by size:
4843 1293 576
MluI A'CGCG_T
Cuts at: 2343 6588 2343
Size: 4245 2467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2911 3097 5826 6010 1305
Size: 1606 186 2729 184 2007
Fragments arranged by size:
2729 2007 1606 186 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2765 10
Size: 54 1425 343 933 3957
Fragments arranged by size:
3957 1425 933 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2697 2925 3221 3266 3390 3591 3872
Size: 648 1531 228 296 45 124 201 281
Cuts at: 3872 3903 4670 5029 5188 518
Size: 31 767 359 159 2042
Fragments arranged by size:
2042 1531 767 648 359 296 281 228
201 159 124 45 31
MspA1I CmG'CkG
Cuts at: 1606 2141 2297 3253 3748 4128 4805 5953 6198
Size: 535 156 956 495 380 677 1148 245
Cuts at: 6198 1606
Size: 2120
Fragments arranged by size:
2120 1148 956 677 535 495 380 245
156
NaeI GCC'GGC
Cuts at: 2062 2111 2136 2196 2062
Size: 49 25 60 6578
Fragments arranged by size:
6578 60 49 25
NarI GG'CG_CC
Cuts at: 2309 2309
Size: 6712
NcoI C'CATG_G
Cuts at: 513 1065 3873 513
Size: 552 2808 3352
Fragments arranged by size:
3352 2808 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6712
NgoMIV G'CCGG_C
Cuts at: 2060 2109 2134 2194 2060
Size: 49 25 60 6578
Fragments arranged by size:
6578 60 49 25
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6712
NotI GC'GGCC_GC
Cuts at: 4159 4159
Size: 6712
NsiI A_TGCA'T
Cuts at: 3387 3387
Size: 6712
NspI r_CATG'y
Cuts at: 1132 1229 3400 4070 5615 1132
Size: 97 2171 670 1545 2229
Fragments arranged by size:
2229 2171 1545 670 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6712
PciI A'CATG_T
Cuts at: 5611 5611
Size: 6712
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3332 1108
Size: 2224 4488
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 6294 6326 4781
Size: 32 1481 32 5167
Fragments arranged by size:
5167 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2038 2038
Size: 6712
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6712
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PspOMI G'GGCC_C
Cuts at: 2143 2143
Size: 6712
PstI C_TGCA'G
Cuts at: 830 3790 4064 830
Size: 2960 274 3478
Fragments arranged by size:
3478 2960 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
PvuII CAG'CTG
Cuts at: 3253 3253
Size: 6712
SacII CC_GC'GG
Cuts at: 2142 2142
Size: 6712
SalI G'TCGA_C
Cuts at: 1936 4054 1936
Size: 2118 4594
SbfI CC_TGCA'GG
Cuts at: 4064 4064
Size: 6712
ScaI AGT'ACT
Cuts at: 3345 4960 6672 3345
Size: 1615 1712 3385
Fragments arranged by size:
3385 1712 1615
SfcI C'TryA_G
Cuts at: 826 1045 2499 3786 4060 5195 5876 6067 826
Size: 219 1454 1287 274 1135 681 191 1471
Fragments arranged by size:
1471 1454 1287 1135 681 274 219 191
SfoI GGC'GCC
Cuts at: 2310 2310
Size: 6712
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 2060 1700
Size: 360 6352
SmaI CCC'GGG
Cuts at: 1872 2281 2332 4039 1872
Size: 409 51 1707 4545
Fragments arranged by size:
4545 1707 409 51
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3948 4816 5717 5979 820
Size: 197 926 2005 868 901 262 1553
Fragments arranged by size:
2005 1553 926 901 868 262 197
SnaBI TAC'GTA
Cuts at: 493 4503 493
Size: 4010 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 4070 1132
Size: 2938 3774
SspI AAT'ATT
Cuts at: 5 52 4636 5
Size: 47 4584 2081
Fragments arranged by size:
4584 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6712
StyI C'CwwG_G
Cuts at: 513 1065 2094 2319 3849 3873 4151 513
Size: 552 1029 225 1530 24 278 3074
Fragments arranged by size:
3074 1530 1029 552 278 225 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4739 4756 4909 5094 5539 325
Size: 1255 3159 17 153 185 445 1498
Fragments arranged by size:
3159 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2801 3343 3939
Size: 274 80 33 51 1443 824 542 596
Cuts at: 3939 4958 6670 96
Size: 1019 1712 138
Fragments arranged by size:
1712 1443 1019 824 596 542 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6672 6704 6672
Size: 32 6680
Tth111I GACn'n_nGTC
Cuts at: 1316 3074 1316
Size: 1758 4954
XbaI T'CTAG_A
Cuts at: 3761 4048 3761
Size: 287 6425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6712
XmaI C'CCGG_G
Cuts at: 1870 2279 2330 4037 1870
Size: 409 51 1707 4545
Fragments arranged by size:
4545 1707 409 51
XmnI GAAnn'nnTTC
Cuts at: 3360 4841 3360
Size: 1481 5231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4520 276
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbvCI Bpu10I BsiWI BstZ17I
EcoICRI EcoNI EcoRV FspAI NruI PacI PfoI PmlI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI XcmI