Restriction map for
pFN21AA0700
(Circular) (Six-base) MAPSORT of: pFN21AA0700.seq Check: 5956 from: 1 to: 8095
pFN21AA0700 8095 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2422 4786 5155 2422
Size: 2364 369 5362
Fragments arranged by size:
5362 2364 369
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4623 5905 278
Size: 53 83 186 936 3087 1282 2468
Fragments arranged by size:
3087 2468 1282 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3801 4221 5438 1937
Size: 1864 420 1217 4594
Fragments arranged by size:
4594 1864 1217 420
Acc65I G'GTAC_C
Cuts at: 2873 5302 5416 2873
Size: 2429 114 5552
Fragments arranged by size:
5552 2429 114
AclI AA'CG_TT
Cuts at: 1563 6222 6595 1563
Size: 4659 373 3063
Fragments arranged by size:
4659 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2652 2978 3650 4316 4436 4649 6158 7542
Size: 1020 326 672 666 120 213 1509 1384
Cuts at: 7542 7810 1632
Size: 268 1917
Fragments arranged by size:
1917 1509 1384 1020 672 666 326 268
213 120
AfeI AGC'GCT
Cuts at: 2810 3380 3909 4742 2810
Size: 570 529 833 6163
Fragments arranged by size:
6163 833 570 529
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7898
AflIII A'CryG_T
Cuts at: 1203 2257 2577 5130 6994 7971 1203
Size: 1054 320 2553 1864 977 1327
Fragments arranged by size:
2553 1864 1327 1054 977 320
AgeI A'CCGG_T
Cuts at: 6926 6926
Size: 8095
AhdI GACnn_n'nnGTC
Cuts at: 6824 6824
Size: 8095
AleI CACnn'nnGTG
Cuts at: 1166 2651 3366 1166
Size: 1485 715 5895
Fragments arranged by size:
5895 1485 715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4602 1184
Size: 3418 4677
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 4673 4705 3305
Size: 32 1336 32 6695
Fragments arranged by size:
6695 1336 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2707 3016 3892 3979 4018 4279 4327 4867 5176
Size: 309 876 87 39 261 48 540 309
Cuts at: 5176 5223 5838 7410 2707
Size: 47 615 1572 3392
Fragments arranged by size:
3392 1572 876 615 540 309 309 261
87 48 47 39
ApaI G_GGCC'C
Cuts at: 5008 5008
Size: 8095
ApaLI G'TGCA_C
Cuts at: 2120 5330 5336 6152 7308 2120
Size: 3210 6 816 1156 2907
Fragments arranged by size:
3210 2907 1156 816 6
ApoI r'AATT_y
Cuts at: 1493 2389 2486 2924 3065 5404 5630 5782 7891
Size: 896 97 438 141 2339 226 152 2109
Cuts at: 7891 1493
Size: 1697
Fragments arranged by size:
2339 2109 1697 896 438 226 152 141
97
AseI AT'TA_AT
Cuts at: 160 1032 6649 160
Size: 872 5617 1606
Fragments arranged by size:
5617 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8095
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 5420 1870
Size: 73 146 3331 4545
Fragments arranged by size:
4545 3331 146 73
BamHI G'GATC_C
Cuts at: 3197 5425 5792 3197
Size: 2228 367 5500
Fragments arranged by size:
5500 2228 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2072 2434 2461 2873
Size: 325 217 627 252 33 362 27 412
Cuts at: 2873 3463 3962 4028 4108 4667 5302 5416 6871
Size: 590 499 66 80 559 635 114 1455
Cuts at: 6871 618
Size: 1842
Fragments arranged by size:
1842 1455 635 627 590 559 499 412
362 325 252 217 114 80 66 33
27
BanII G_rGCy'C
Cuts at: 1391 2096 3036 4644 4818 5008 5030 5414 1391
Size: 705 940 1608 174 190 22 384 4072
Fragments arranged by size:
4072 1608 940 705 384 190 174 22
BbeI G_GCGC'C
Cuts at: 2076 3467 4032 4112 2076
Size: 1391 565 80 6059
Fragments arranged by size:
6059 1391 565 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 3344 3477 928
Size: 971 237 1208 133 5546
Fragments arranged by size:
5546 1208 971 237 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4853 4887 6286 6320 1903
Size: 34 2916 34 1399 34 3678
Fragments arranged by size:
3678 2916 1399 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5987 7197 5987
Size: 1210 6885
BclI T'GATC_A
Cuts at: 1548 4800 1548
Size: 3252 4843
BfrBI ATG'CAT
Cuts at: 3179 3179
Size: 8095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2055 6706 136
Size: 107 122 71 631 605 383 4651 1525
Fragments arranged by size:
4651 1525 631 605 383 122 107 71
BglII A'GATC_T
Cuts at: 3678 4299 8090 3678
Size: 621 3791 3683
Fragments arranged by size:
3791 3683 621
BlpI GC'TnA_GC
Cuts at: 2066 4737 5087 5512 2066
Size: 2671 350 425 4649
Fragments arranged by size:
4649 2671 425 350
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2466 3214 3844 3965 3996 5008 5334
Size: 334 342 748 630 121 31 1012 326
Cuts at: 5334 5340 6156 7312 1790
Size: 6 816 1156 2573
Fragments arranged by size:
2573 1156 1012 816 748 630 342 334
326 121 31 6
BmgBI CAC'GTC
Cuts at: 1318 2258 3513 5068 1318
Size: 940 1255 1555 4345
Fragments arranged by size:
4345 1555 1255 940
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4083 4419 5826
Size: 416 53 83 399 24 2666 336 1407
Cuts at: 5826 6784 442
Size: 958 1753
Fragments arranged by size:
2666 1753 1407 958 416 399 336 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 5259 1056
Size: 4203 3892
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3841 3841
Size: 8095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2265 2489 2585 3422 3914 4270 4349
Size: 983 43 224 96 837 492 356 79
Cuts at: 4349 4460 4505 5249 6755 1239
Size: 111 45 744 1506 2579
Fragments arranged by size:
2579 1506 983 837 744 492 356 224
111 96 79 45 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3569 3695 4949 6220 7085 7383 3569
Size: 126 1254 1271 865 298 4281
Fragments arranged by size:
4281 1271 1254 865 298 126
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2730 3295 4709 6758 882
Size: 617 1231 565 1414 2049 2219
Fragments arranged by size:
2219 2049 1414 1231 617 565
BsaAI yAC'GTr
Cuts at: 493 1204 2125 2580 3925 4552 4879 5335 5886
Size: 711 921 455 1345 627 327 456 551
Cuts at: 5886 493
Size: 2702
Fragments arranged by size:
2702 1345 921 711 627 551 456 455
327
BsaBI GATnn'nnATC
Cuts at: 8089 8089
Size: 8095
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2133 3464 4029
Size: 53 83 186 936 540 60 1331 565
Cuts at: 4029 4109 4620 5902 6284 6919 275
Size: 80 511 1282 382 635 1451
Fragments arranged by size:
1451 1331 1282 936 635 565 540 511
382 186 83 80 60 53
BsaWI w'CCGG_w
Cuts at: 6528 6926 7200 7347 7758 6528
Size: 398 274 147 411 6865
Fragments arranged by size:
6865 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3005 3035 4673 4703 1178
Size: 30 165 30 1602 30 1638 30 4570
Fragments arranged by size:
4570 1638 1602 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2651 2816 3365 3659 4052 4151 4457 4844
Size: 1465 165 549 294 393 99 306 387
Cuts at: 4844 5222 5300 1186
Size: 378 78 3981
Fragments arranged by size:
3981 1465 549 393 387 378 306 294
165 99 78
BseYI C'CCAG_C
Cuts at: 2079 3660 3995 4192 7298 7794 2079
Size: 1581 335 197 3106 496 2380
Fragments arranged by size:
3106 2380 1581 496 335 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2433 2558 2786 2826 3262 3461 3965
Size: 573 697 125 228 40 436 199 504
Cuts at: 3965 4253 4583 4598 5189 5285 5387 1163
Size: 288 330 15 591 96 102 3871
Fragments arranged by size:
3871 697 591 573 504 436 330 288
228 199 125 102 96 40 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5545 6306 7334 1540
Size: 275 186 3544 761 1028 2301
Fragments arranged by size:
3544 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2283 2781 2799 3036 3813 4286 4644 4818
Size: 159 498 18 237 777 473 358 174
Cuts at: 4818 5030 5334 5340 6156 6241 7312 2124
Size: 212 304 6 816 85 1071 2907
Fragments arranged by size:
2907 1071 816 777 498 473 358 304
237 212 174 159 85 18 6
BsiWI C'GTAC_G
Cuts at: 3345 3345
Size: 8095
BsmI GAATG_Cn'
Cuts at: 5606 5699 5606
Size: 93 8002
BsmBI CGTCTCn'nnnn_
Cuts at: 3398 3398
Size: 8095
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2124 2283 2466 2781 2799 3036
Size: 399 306 28 159 183 315 18 237
Cuts at: 3036 3214 3813 3844 3965 3996 4286 4644 4818
Size: 178 599 31 121 31 290 358 174
Cuts at: 4818 5008 5030 5334 5340 5414 6156 6241 7312
Size: 190 22 304 6 74 742 85 1071
Cuts at: 7312 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 599 399 358 315 306
304 290 237 190 183 178 174 159
121 85 74 31 31 28 22 18
6
BspEI T'CCGG_A
Cuts at: 7758 7758
Size: 8095
BspHI T'CATG_A
Cuts at: 2208 4803 5984 7714 2208
Size: 2595 1181 1730 2589
Fragments arranged by size:
2595 2589 1730 1181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2422 2691 4786 5155 5450 844
Size: 1578 269 2095 369 295 3489
Fragments arranged by size:
3489 2095 1578 369 295 269
BsrBI CCG'CTC
Cuts at: 1394 1823 2070 4096 4351 5982 1394
Size: 429 247 2026 255 1631 3507
Fragments arranged by size:
3507 2026 1631 429 255 247
BsrDI GCAATG_nn'
Cuts at: 65 1248 4584 6590 6764 65
Size: 1183 3336 2006 174 1396
Fragments arranged by size:
3336 2006 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2116 2610 3882 3937 4105 4980
Size: 110 232 74 494 1272 55 168 875
Cuts at: 4980 6739 6926 1700
Size: 1759 187 2869
Fragments arranged by size:
2869 1759 1272 875 494 232 187 168
110 74 55
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8095
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8095
BssSI C'ACGA_G
Cuts at: 2105 2276 2602 4607 6155 7167 2105
Size: 171 326 2005 1548 1012 3033
Fragments arranged by size:
3033 2005 1548 1012 326 171
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2434 2764 5344 794
Size: 1640 330 2580 3545
Fragments arranged by size:
3545 2580 1640 330
BstBI TT'CG_AA
Cuts at: 6899 6899
Size: 8095
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8095
BstXI CCAn_nnnn'nTGG
Cuts at: 4432 4432
Size: 8095
BstYI r'GATC_y
Cuts at: 1972 2160 2568 3143 3197 3678 3888 4299 5425
Size: 188 408 575 54 481 210 411 1126
Cuts at: 5425 5792 6177 6194 7646 7732 7744 8090 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1126 575 481 411 408 385
367 346 210 188 86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2401 2481 4401 4563 513
Size: 552 939 48 349 80 1920 162 4045
Fragments arranged by size:
4045 1920 939 552 349 162 80 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2875 2980 4629 487
Size: 683 56 1649 105 1649 3953
Fragments arranged by size:
3953 1649 1649 683 105 56
BtsI GCAGTG_nn'
Cuts at: 2904 3250 3844 5611 6405 6425 2904
Size: 346 594 1767 794 20 4574
Fragments arranged by size:
4574 1767 794 594 346 20
ClaI AT'CG_AT
Cuts at: 1554 2235 1554
Size: 681 7414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3226 3261 529
Size: 35 2662 35 5363
Fragments arranged by size:
5363 2662 35 35
DraI TTT'AAA
Cuts at: 5399 5399
Size: 8095
DraIII CAC_nnn'GTG
Cuts at: 2764 4609 2764
Size: 1845 6250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3287 7102 809
Size: 507 1971 3815 1802
Fragments arranged by size:
3815 1971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2612 2655 3414
Size: 54 1425 50 275 18 782 43 759
Cuts at: 3414 3939 4164 4749 5542 6431 8060 8
Size: 525 225 585 793 889 1629 43
Fragments arranged by size:
1629 1425 889 793 782 759 585 525
275 225 54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5542 1537
Size: 275 3730 4090
Fragments arranged by size:
4090 3730 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 2628 2669 2954 3008 3636 4007 6025
Size: 923 352 41 285 54 628 371 2018
Cuts at: 6025 1353
Size: 3423
Fragments arranged by size:
3423 2018 923 628 371 352 285 54
41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2819 2936 4964 5177 6682 7056
Size: 1064 138 1230 117 2028 213 1505 374
Cuts at: 7056 7202 387
Size: 146 1280
Fragments arranged by size:
2028 1505 1280 1230 1064 374 213 146
138 117
EcoICRI GAG'CTC
Cuts at: 3034 4642 4816 5028 3034
Size: 1608 174 212 6101
Fragments arranged by size:
6101 1608 212 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2265 2489 2585 2652 2978 3422
Size: 393 590 43 224 96 67 326 444
Cuts at: 3422 3650 3914 4270 4316 4349 4436 4460 4505
Size: 228 264 356 46 33 87 24 45
Cuts at: 4505 4649 5249 6158 6755 7542 7810 1239
Size: 144 600 909 597 787 268 1524
Fragments arranged by size:
1524 909 787 600 597 590 444 393
356 326 268 264 228 224 144 96
87 67 46 45 43 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 4484 5264 4484
Size: 780 7315
EcoO109I rG'GnC_Cy
Cuts at: 2680 3562 3760 4115 4146 4846 5005 5145 2680
Size: 882 198 355 31 700 159 140 5630
Fragments arranged by size:
5630 882 700 355 198 159 140 31
EcoRI G'AATT_C
Cuts at: 2389 2486 5404 5782 2389
Size: 97 2918 378 4702
Fragments arranged by size:
4702 2918 378 97
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 4165 2182
Size: 1983 6112
FseI GG_CCGG'CC
Cuts at: 2614 2614
Size: 8095
FspI TGC'GCA
Cuts at: 6601 6601
Size: 8095
HaeII r_GCGC'y
Cuts at: 1678 2076 2812 3382 3467 3532 3911 4032 4112
Size: 398 736 570 85 65 379 121 80
Cuts at: 4112 4744 5061 7242 1678
Size: 632 317 2181 2531
Fragments arranged by size:
2531 2181 736 632 570 398 379 317
121 85 80 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2704 3005 3037 3164
Size: 32 135 32 1100 32 301 32 127
Cuts at: 3164 3196 4892 4924 6736 6768 6810 6842 1373
Size: 32 1696 32 1812 32 42 32 2626
Fragments arranged by size:
2626 1812 1696 1100 301 135 127 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5439 5685 669
Size: 988 281 3501 246 3079
Fragments arranged by size:
3501 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8095
HpaI GTT'AAC
Cuts at: 5685 5685
Size: 8095
KasI G'GCGC_C
Cuts at: 2072 3463 4028 4108 2072
Size: 1391 565 80 6059
Fragments arranged by size:
6059 1391 565 80
KpnI G_GTAC'C
Cuts at: 2877 5306 5420 2877
Size: 2429 114 5552
Fragments arranged by size:
5552 2429 114
MfeI C'AATT_G
Cuts at: 5694 5694
Size: 8095
MluI A'CGCG_T
Cuts at: 7971 7971
Size: 8095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2278 3411 3495 7209 7393 1305
Size: 973 1133 84 3714 184 2007
Fragments arranged by size:
3714 2007 1133 973 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2657 3416 10
Size: 54 1425 343 825 759 4689
Fragments arranged by size:
4689 1425 825 759 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2651 3366 3869 6053 6412 6571 518
Size: 648 1485 715 503 2184 359 159 2042
Fragments arranged by size:
2184 2042 1485 715 648 503 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2037 2054 2079 2596 2991 3366 3605
Size: 422 9 17 25 517 395 375 239
Cuts at: 3605 3976 4018 4046 4597 4673 4695 5223 5511
Size: 371 42 28 551 76 22 528 288
Cuts at: 5511 6188 7336 7581 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 551 528 517 422 395
375 371 288 245 239 76 42 28
25 22 17 9
NaeI GCC'GGC
Cuts at: 2044 2612 3939 4107 2044
Size: 568 1327 168 6032
Fragments arranged by size:
6032 1327 568 168
NarI GG'CG_CC
Cuts at: 2073 3464 4029 4109 2073
Size: 1391 565 80 6059
Fragments arranged by size:
6059 1391 565 80
NcoI C'CATG_G
Cuts at: 513 1065 2004 4401 4563 513
Size: 552 939 2397 162 4045
Fragments arranged by size:
4045 2397 939 552 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8095
NgoMIV G'CCGG_C
Cuts at: 2042 2610 3937 4105 2042
Size: 568 1327 168 6032
Fragments arranged by size:
6032 1327 568 168
NheI G'CTAG_C
Cuts at: 1052 5255 1052
Size: 4203 3892
NotI GC'GGCC_GC
Cuts at: 5542 5542
Size: 8095
NsiI A_TGCA'T
Cuts at: 3181 3181
Size: 8095
NspI r_CATG'y
Cuts at: 1132 1229 2629 5134 5453 6998 1132
Size: 97 1400 2505 319 1545 2229
Fragments arranged by size:
2505 2229 1545 1400 319 97
PasI CC'CwG_GG
Cuts at: 1348 3502 4069 1348
Size: 2154 567 5374
Fragments arranged by size:
5374 2154 567
PciI A'CATG_T
Cuts at: 5130 6994 5130
Size: 1864 6231
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3016 4867 1108
Size: 1908 1851 4336
Fragments arranged by size:
4336 1908 1851
PfoI T'CCnGG_A
Cuts at: 2199 2242 2562 3399 3891 4461 4818 5030 2199
Size: 43 320 837 492 570 357 212 5264
Fragments arranged by size:
5264 837 570 492 357 320 212 43
PmeI GTTT'AAAC
Cuts at: 5399 5399
Size: 8095
PmlI CAC'GTG
Cuts at: 5335 5335
Size: 8095
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2659 4673 4705 6164 6196 7677 7709 2627
Size: 32 2014 32 1459 32 1481 32 3013
Fragments arranged by size:
3013 2014 1481 1459 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2680 4115 4846 5145 2680
Size: 1435 731 299 5630
Fragments arranged by size:
5630 1435 731 299
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8095
PsiI TTA'TAA
Cuts at: 5665 5665
Size: 8095
PspOMI G'GGCC_C
Cuts at: 5004 5004
Size: 8095
PstI C_TGCA'G
Cuts at: 830 2899 2922 3042 4785 4956 5447 830
Size: 2069 23 120 1743 171 491 3478
Fragments arranged by size:
3478 2069 1743 491 171 120 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8095
PvuII CAG'CTG
Cuts at: 2596 3976 4018 4046 4597 2596
Size: 1380 42 28 551 6094
Fragments arranged by size:
6094 1380 551 42 28
RsrII CG'GwC_CG
Cuts at: 4338 4338
Size: 8095
SacI G_AGCT'C
Cuts at: 3036 4644 4818 5030 3036
Size: 1608 174 212 6101
Fragments arranged by size:
6101 1608 212 174
SacII CC_GC'GG
Cuts at: 2055 2055
Size: 8095
SalI G'TCGA_C
Cuts at: 1936 5437 1936
Size: 3501 4594
SanDI GG'GwC_CC
Cuts at: 5145 5145
Size: 8095
SapI GCTCTTCn'nnn_
Cuts at: 2276 2954 2276
Size: 678 7417
SbfI CC_TGCA'GG
Cuts at: 5447 5447
Size: 8095
ScaI AGT'ACT
Cuts at: 4448 6343 8055 4448
Size: 1895 1712 4488
Fragments arranged by size:
4488 1895 1712
SexAI A'CCwGG_T
Cuts at: 2151 2151
Size: 8095
SfcI C'TryA_G
Cuts at: 826 1045 2551 2895 2918 3038 4222 4781 4952
Size: 219 1506 344 23 120 1184 559 171
Cuts at: 4952 5443 6578 7259 7450 826
Size: 491 1135 681 191 1471
Fragments arranged by size:
1506 1471 1184 1135 681 559 491 344
219 191 171 120 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 2055 2055
Size: 8095
SfoI GGC'GCC
Cuts at: 2074 3465 4030 4110 2074
Size: 1391 565 80 6059
Fragments arranged by size:
6059 1391 565 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8095
SgrAI Cr'CCGG_yG
Cuts at: 1700 4980 1700
Size: 3280 4815
SmaI CCC'GGG
Cuts at: 1872 5422 1872
Size: 3550 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3584 3710 4964 6199 7100 7362
Size: 197 926 1641 126 1254 1235 901 262
Cuts at: 7362 820
Size: 1553
Fragments arranged by size:
1641 1553 1254 1235 926 901 262 197
126
SnaBI TAC'GTA
Cuts at: 493 5886 493
Size: 5393 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8095
SphI G_CATG'C
Cuts at: 1132 5453 1132
Size: 4321 3774
SspI AAT'ATT
Cuts at: 5 52 6019 5
Size: 47 5967 2081
Fragments arranged by size:
5967 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8095
StyI C'CwwG_G
Cuts at: 513 1065 2004 3636 3756 4401 4563 5534 513
Size: 552 939 1632 120 645 162 971 3074
Fragments arranged by size:
3074 1632 971 939 645 552 162 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2556 3145 4355 4913 5270 6122 6139
Size: 1255 976 589 1210 558 357 852 17
Cuts at: 6139 6292 6477 6922 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1210 976 852 589 558 445
357 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4446 5370 6341
Size: 274 80 33 51 1443 2469 924 971
Cuts at: 6341 8053 96
Size: 1712 138
Fragments arranged by size:
2469 1712 1443 971 924 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 8055 8087 3632
Size: 32 4391 32 3640
Fragments arranged by size:
4391 3640 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2942 3395 4217 1316
Size: 1626 453 822 5194
Fragments arranged by size:
5194 1626 822 453
XbaI T'CTAG_A
Cuts at: 5431 5431
Size: 8095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2896 4570 4816 2896
Size: 1674 246 6175
Fragments arranged by size:
6175 1674 246
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8095
XmaI C'CCGG_G
Cuts at: 1870 5420 1870
Size: 3550 4545
XmnI GAAnn'nnTTC
Cuts at: 2216 4763 6224 2216
Size: 2547 1461 4087
Fragments arranged by size:
4087 2547 1461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4621 5903 276
Size: 53 83 186 936 3087 1282 2468
Fragments arranged by size:
3087 2468 1282 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI Bpu10I BstZ17I Bsu36I EcoRV
FspAI NruI PacI PspXI PsrI SrfI SwaI