Restriction map for
pFN21AA0700
(Linear) (Six-base) MAPSORT of: KIAA0700.seq Check: 3031 from: 1 to: 3390
KIAA0700.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 417 2781 3150 3390
Size: 417 2364 369 240
Fragments arranged by size:
2364 417 369 240
AatII G_ACGT'C
Cuts at: 0 2618 3390
Size: 2618 772
AccI GT'mk_AC
Cuts at: 0 1796 2216 3390
Size: 1796 420 1174
Fragments arranged by size:
1796 1174 420
Acc65I G'GTAC_C
Cuts at: 0 868 3297 3390
Size: 868 2429 93
Fragments arranged by size:
2429 868 93
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 973 1645 2311 2431 2644 3390
Size: 647 326 672 666 120 213 746
Fragments arranged by size:
746 672 666 647 326 213 120
AfeI AGC'GCT
Cuts at: 0 805 1375 1904 2737 3390
Size: 805 570 529 833 653
Fragments arranged by size:
833 805 653 570 529
AflIII A'CryG_T
Cuts at: 0 252 572 3125 3390
Size: 252 320 2553 265
Fragments arranged by size:
2553 320 265 252
AleI CACnn'nnGTG
Cuts at: 0 646 1361 3390
Size: 646 715 2029
Fragments arranged by size:
2029 715 646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2597 3390
Size: 2597 793
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 2668 2700 3390
Size: 1300 32 1336 32 690
Fragments arranged by size:
1336 1300 690 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 702 1011 1887 1974 2013 2274 2322 2862
Size: 702 309 876 87 39 261 48 540
Cuts at: 2862 3171 3218 3390
Size: 309 47 172
Fragments arranged by size:
876 702 540 309 309 261 172 87
48 47 39
ApaI G_GGCC'C
Cuts at: 0 3003 3390
Size: 3003 387
ApaLI G'TGCA_C
Cuts at: 0 115 3325 3331 3390
Size: 115 3210 6 59
Fragments arranged by size:
3210 115 59 6
ApoI r'AATT_y
Cuts at: 0 384 481 919 1060 3390
Size: 384 97 438 141 2330
Fragments arranged by size:
2330 438 384 141 97
AvaI C'yCGr_G
Cuts at: 0 84 3390
Size: 84 3306
BamHI G'GATC_C
Cuts at: 0 1192 3390
Size: 1192 2198
BanI G'GyrC_C
Cuts at: 0 34 67 429 456 868 1458 1957 2023
Size: 34 33 362 27 412 590 499 66
Cuts at: 2023 2103 2662 3297 3390
Size: 80 559 635 93
Fragments arranged by size:
635 590 559 499 412 362 93 80
66 34 33 27
BanII G_rGCy'C
Cuts at: 0 91 1031 2639 2813 3003 3025 3390
Size: 91 940 1608 174 190 22 365
Fragments arranged by size:
1608 940 365 190 174 91 22
BbeI G_GCGC'C
Cuts at: 0 71 1462 2027 2107 3390
Size: 71 1391 565 80 1283
Fragments arranged by size:
1391 1283 565 80 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 1339 1472 3390
Size: 131 1208 133 1918
Fragments arranged by size:
1918 1208 133 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2848 2882 3390
Size: 2848 34 508
Fragments arranged by size:
2848 508 34
BclI T'GATC_A
Cuts at: 0 2795 3390
Size: 2795 595
BfrBI ATG'CAT
Cuts at: 0 1174 3390
Size: 1174 2216
BglI GCCn_nnn'nGGC
Cuts at: 0 50 3390
Size: 50 3340
BglII A'GATC_T
Cuts at: 0 1673 2294 3390
Size: 1673 621 1096
Fragments arranged by size:
1673 1096 621
BlpI GC'TnA_GC
Cuts at: 0 61 2732 3082 3390
Size: 61 2671 350 308
Fragments arranged by size:
2671 350 308 61
Bme1580I G_kGCm'C
Cuts at: 0 119 461 1209 1839 1960 1991 3003 3329
Size: 119 342 748 630 121 31 1012 326
Cuts at: 3329 3335 3390
Size: 6 55
Fragments arranged by size:
1012 748 630 342 326 121 119 55
31 6
BmgBI CAC'GTC
Cuts at: 0 253 1508 3063 3390
Size: 253 1255 1555 327
Fragments arranged by size:
1555 1255 327 253
BmrI ACTGGGnnnn_n'
Cuts at: 0 2078 2414 3390
Size: 2078 336 976
Fragments arranged by size:
2078 976 336
BmtI G_CTAG'C
Cuts at: 0 3254 3390
Size: 3254 136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1836 3390
Size: 1836 1554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 260 484 580 1417 1909 2265 2344
Size: 217 43 224 96 837 492 356 79
Cuts at: 2344 2455 2500 3244 3390
Size: 111 45 744 146
Fragments arranged by size:
837 744 492 356 224 217 146 111
96 79 45 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1564 1690 2944 3390
Size: 1564 126 1254 446
Fragments arranged by size:
1564 1254 446 126
BsaI GGTCTCn'nnnn_
Cuts at: 0 725 1290 2704 3390
Size: 725 565 1414 686
Fragments arranged by size:
1414 725 686 565
BsaAI yAC'GTr
Cuts at: 0 120 575 1920 2547 2874 3330 3390
Size: 120 455 1345 627 327 456 60
Fragments arranged by size:
1345 627 456 455 327 120 60
BsaHI Gr'CG_yC
Cuts at: 0 68 128 1459 2024 2104 2615 3390
Size: 68 60 1331 565 80 511 775
Fragments arranged by size:
1331 775 565 511 80 68 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1000 1030 2668 2698 3390
Size: 1000 30 1638 30 692
Fragments arranged by size:
1638 1000 692 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 811 1360 1654 2047 2146 2452 2839
Size: 646 165 549 294 393 99 306 387
Cuts at: 2839 3217 3295 3390
Size: 378 78 95
Fragments arranged by size:
646 549 393 387 378 306 294 165
99 95 78
BseYI C'CCAG_C
Cuts at: 0 74 1655 1990 2187 3390
Size: 74 1581 335 197 1203
Fragments arranged by size:
1581 1203 335 197 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 553 781 821 1257 1456 1960 2248
Size: 428 125 228 40 436 199 504 288
Cuts at: 2248 2578 2593 3184 3280 3382 3390
Size: 330 15 591 96 102 8
Fragments arranged by size:
591 504 436 428 330 288 228 199
125 102 96 40 15 8
BsiHKAI G_wGCw'C
Cuts at: 0 119 278 776 794 1031 1808 2281 2639
Size: 119 159 498 18 237 777 473 358
Cuts at: 2639 2813 3025 3329 3335 3390
Size: 174 212 304 6 55
Fragments arranged by size:
777 498 473 358 304 237 212 174
159 119 55 18 6
BsiWI C'GTAC_G
Cuts at: 0 1340 3390
Size: 1340 2050
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1393 3390
Size: 1393 1997
Bsp1286I G_dGCh'C
Cuts at: 0 91 119 278 461 776 794 1031 1209
Size: 91 28 159 183 315 18 237 178
Cuts at: 1209 1808 1839 1960 1991 2281 2639 2813 3003
Size: 599 31 121 31 290 358 174 190
Cuts at: 3003 3025 3329 3335 3390
Size: 22 304 6 55
Fragments arranged by size:
599 358 315 304 290 237 190 183
178 174 159 121 91 55 31 31
28 22 18 6
BspHI T'CATG_A
Cuts at: 0 203 2798 3390
Size: 203 2595 592
Fragments arranged by size:
2595 592 203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 417 686 2781 3150 3390
Size: 417 269 2095 369 240
Fragments arranged by size:
2095 417 369 269 240
BsrBI CCG'CTC
Cuts at: 0 65 2091 2346 3390
Size: 65 2026 255 1044
Fragments arranged by size:
2026 1044 255 65
BsrDI GCAATG_nn'
Cuts at: 0 2579 3390
Size: 2579 811
BsrFI r'CCGG_y
Cuts at: 0 37 111 605 1877 1932 2100 2975 3390
Size: 37 74 494 1272 55 168 875 415
Fragments arranged by size:
1272 875 494 415 168 74 55 37
BssSI C'ACGA_G
Cuts at: 0 100 271 597 2602 3390
Size: 100 171 326 2005 788
Fragments arranged by size:
2005 788 326 171 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 429 759 3339 3390
Size: 429 330 2580 51
Fragments arranged by size:
2580 429 330 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2427 3390
Size: 2427 963
BstYI r'GATC_y
Cuts at: 0 155 563 1138 1192 1673 1883 2294 3390
Size: 155 408 575 54 481 210 411 1096
Fragments arranged by size:
1096 575 481 411 408 210 155 54
BtgI C'CryG_G
Cuts at: 0 47 396 476 2396 2558 3390
Size: 47 349 80 1920 162 832
Fragments arranged by size:
1920 832 349 162 80 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 870 975 2624 3390
Size: 870 105 1649 766
Fragments arranged by size:
1649 870 766 105
BtsI GCAGTG_nn'
Cuts at: 0 899 1245 1839 3390
Size: 899 346 594 1551
Fragments arranged by size:
1551 899 594 346
ClaI AT'CG_AT
Cuts at: 0 230 3390
Size: 230 3160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1221 1256 3390
Size: 1221 35 2134
Fragments arranged by size:
2134 1221 35
DraIII CAC_nnn'GTG
Cuts at: 0 759 2604 3390
Size: 759 1845 786
Fragments arranged by size:
1845 786 759
DrdI GACnn_nn'nnGTC
Cuts at: 0 1282 3390
Size: 1282 2108
EaeI y'GGCC_r
Cuts at: 0 607 650 1409 1934 2159 2744 3390
Size: 607 43 759 525 225 585 646
Fragments arranged by size:
759 646 607 585 525 225 43
EarI CTCTTCn'nnn_
Cuts at: 0 271 623 664 949 1003 1631 2002 3390
Size: 271 352 41 285 54 628 371 1388
Fragments arranged by size:
1388 628 371 352 285 271 54 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 814 931 2959 3172 3390
Size: 814 117 2028 213 218
Fragments arranged by size:
2028 814 218 213 117
EcoICRI GAG'CTC
Cuts at: 0 1029 2637 2811 3023 3390
Size: 1029 1608 174 212 367
Fragments arranged by size:
1608 1029 367 212 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 260 484 580 647 973 1417 1645
Size: 217 43 224 96 67 326 444 228
Cuts at: 1645 1909 2265 2311 2344 2431 2455 2500 2644
Size: 264 356 46 33 87 24 45 144
Cuts at: 2644 3244 3390
Size: 600 146
Fragments arranged by size:
600 444 356 326 264 228 224 217
146 144 96 87 67 46 45 43
33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2479 3259 3390
Size: 2479 780 131
Fragments arranged by size:
2479 780 131
EcoO109I rG'GnC_Cy
Cuts at: 0 675 1557 1755 2110 2141 2841 3000 3140
Size: 675 882 198 355 31 700 159 140
Cuts at: 3140 3390
Size: 250
Fragments arranged by size:
882 700 675 355 250 198 159 140
31
EcoRI G'AATT_C
Cuts at: 0 384 481 3390
Size: 384 97 2909
Fragments arranged by size:
2909 384 97
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 177 2160 3390
Size: 177 1983 1230
Fragments arranged by size:
1983 1230 177
FseI GG_CCGG'CC
Cuts at: 0 609 3390
Size: 609 2781
HaeII r_GCGC'y
Cuts at: 0 71 807 1377 1462 1527 1906 2027 2107
Size: 71 736 570 85 65 379 121 80
Cuts at: 2107 2739 3056 3390
Size: 632 317 334
Fragments arranged by size:
736 632 570 379 334 317 121 85
80 71 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 1000 1032 1159 1191 2887 2919
Size: 667 32 301 32 127 32 1696 32
Cuts at: 2919 3390
Size: 471
Fragments arranged by size:
1696 667 471 301 127 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 67 1458 2023 2103 3390
Size: 67 1391 565 80 1287
Fragments arranged by size:
1391 1287 565 80 67
KpnI G_GTAC'C
Cuts at: 0 872 3301 3390
Size: 872 2429 89
Fragments arranged by size:
2429 872 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 1406 1490 3390
Size: 273 1133 84 1900
Fragments arranged by size:
1900 1133 273 84
MscI TGG'CCA
Cuts at: 0 652 1411 3390
Size: 652 759 1979
Fragments arranged by size:
1979 759 652
MslI CAynn'nnrTG
Cuts at: 0 646 1361 1864 3390
Size: 646 715 503 1526
Fragments arranged by size:
1526 715 646 503
MspA1I CmG'CkG
Cuts at: 0 23 32 49 74 591 986 1361 1600
Size: 23 9 17 25 517 395 375 239
Cuts at: 1600 1971 2013 2041 2592 2668 2690 3218 3390
Size: 371 42 28 551 76 22 528 172
Fragments arranged by size:
551 528 517 395 375 371 239 172
76 42 28 25 23 22 17 9
NaeI GCC'GGC
Cuts at: 0 39 607 1934 2102 3390
Size: 39 568 1327 168 1288
Fragments arranged by size:
1327 1288 568 168 39
NarI GG'CG_CC
Cuts at: 0 68 1459 2024 2104 3390
Size: 68 1391 565 80 1286
Fragments arranged by size:
1391 1286 565 80 68
NcoI C'CATG_G
Cuts at: 0 2396 2558 3390
Size: 2396 162 832
Fragments arranged by size:
2396 832 162
NgoMIV G'CCGG_C
Cuts at: 0 37 605 1932 2100 3390
Size: 37 568 1327 168 1290
Fragments arranged by size:
1327 1290 568 168 37
NheI G'CTAG_C
Cuts at: 0 3250 3390
Size: 3250 140
NsiI A_TGCA'T
Cuts at: 0 1176 3390
Size: 1176 2214
NspI r_CATG'y
Cuts at: 0 624 3129 3390
Size: 624 2505 261
Fragments arranged by size:
2505 624 261
PasI CC'CwG_GG
Cuts at: 0 1497 2064 3390
Size: 1497 567 1326
Fragments arranged by size:
1497 1326 567
PciI A'CATG_T
Cuts at: 0 3125 3390
Size: 3125 265
PflMI CCAn_nnn'nTGG
Cuts at: 0 1011 2862 3390
Size: 1011 1851 528
Fragments arranged by size:
1851 1011 528
PfoI T'CCnGG_A
Cuts at: 0 194 237 557 1394 1886 2456 2813 3025
Size: 194 43 320 837 492 570 357 212
Cuts at: 3025 3390
Size: 365
Fragments arranged by size:
837 570 492 365 357 320 212 194
43
PmlI CAC'GTG
Cuts at: 0 3330 3390
Size: 3330 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 2668 2700 3390
Size: 622 32 2014 32 690
Fragments arranged by size:
2014 690 622 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 675 2110 2841 3140 3390
Size: 675 1435 731 299 250
Fragments arranged by size:
1435 731 675 299 250
PspOMI G'GGCC_C
Cuts at: 0 2999 3390
Size: 2999 391
PstI C_TGCA'G
Cuts at: 0 894 917 1037 2780 2951 3390
Size: 894 23 120 1743 171 439
Fragments arranged by size:
1743 894 439 171 120 23
PvuII CAG'CTG
Cuts at: 0 591 1971 2013 2041 2592 3390
Size: 591 1380 42 28 551 798
Fragments arranged by size:
1380 798 591 551 42 28
RsrII CG'GwC_CG
Cuts at: 0 2333 3390
Size: 2333 1057
SacI G_AGCT'C
Cuts at: 0 1031 2639 2813 3025 3390
Size: 1031 1608 174 212 365
Fragments arranged by size:
1608 1031 365 212 174
SacII CC_GC'GG
Cuts at: 0 50 3390
Size: 50 3340
SanDI GG'GwC_CC
Cuts at: 0 3140 3390
Size: 3140 250
SapI GCTCTTCn'nnn_
Cuts at: 0 271 949 3390
Size: 271 678 2441
Fragments arranged by size:
2441 678 271
ScaI AGT'ACT
Cuts at: 0 2443 3390
Size: 2443 947
SexAI A'CCwGG_T
Cuts at: 0 146 3390
Size: 146 3244
SfcI C'TryA_G
Cuts at: 0 546 890 913 1033 2217 2776 2947 3390
Size: 546 344 23 120 1184 559 171 443
Fragments arranged by size:
1184 559 546 443 344 171 120 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 50 3390
Size: 50 3340
SfoI GGC'GCC
Cuts at: 0 69 1460 2025 2105 3390
Size: 69 1391 565 80 1285
Fragments arranged by size:
1391 1285 565 80 69
SgrAI Cr'CCGG_yG
Cuts at: 0 2975 3390
Size: 2975 415
SmlI C'TyrA_G
Cuts at: 0 1579 1705 2959 3390
Size: 1579 126 1254 431
Fragments arranged by size:
1579 1254 431 126
StyI C'CwwG_G
Cuts at: 0 1631 1751 2396 2558 3390
Size: 1631 120 645 162 832
Fragments arranged by size:
1631 832 645 162 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 551 1140 2350 2908 3265 3390
Size: 551 589 1210 558 357 125
Fragments arranged by size:
1210 589 558 551 357 125
TatI w'GTAC_w
Cuts at: 0 2441 3365 3390
Size: 2441 924 25
Fragments arranged by size:
2441 924 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1627 1659 3390
Size: 1627 32 1731
Fragments arranged by size:
1731 1627 32
Tth111I GACn'n_nGTC
Cuts at: 0 937 1390 2212 3390
Size: 937 453 822 1178
Fragments arranged by size:
1178 937 822 453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 891 2565 2811 3390
Size: 891 1674 246 579
Fragments arranged by size:
1674 891 579 246
XmnI GAAnn'nnTTC
Cuts at: 0 211 2758 3390
Size: 211 2547 632
Fragments arranged by size:
2547 632 211
ZraI GAC'GTC
Cuts at: 0 2616 3390
Size: 2616 774
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII RsrII
SacI SacII SanDI SapI ScaI SexAI SfcI SfiI
SfoI SgrAI SmlI StyI TaqII TatI TstI Tth111I
XcmI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AscI AseI AsiSI AvrII
BaeI BbvCI BciVI Bpu10I BsaBI BsaWI BsiEI BsmI
BspEI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I DraI
EagI EcoRV FspI FspAI HincII HindIII HpaI MfeI
MluI NdeI NotI NruI PacI PmeI PshAI PsiI
PspXI PsrI PvuI SalI SbfI SgfI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XhoI
XmaI