(Circular) (Six-base) MAPSORT of: pFN21AA0628.seq Check: 4441 from: 1 to: 6313 pFN21AA0628 6313 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2233 2233 Size: 6313 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2108 3083 4123 278 Size: 53 83 186 936 572 975 1040 2468 Fragments arranged by size: 2468 1040 975 936 572 186 83 53 AccI GT'mk_AC Cuts at: 1937 3656 1937 Size: 1719 4594 Acc65I G'GTAC_C Cuts at: 3634 3634 Size: 6313 AclI AA'CG_TT Cuts at: 1563 3507 4440 4813 1563 Size: 1944 933 373 3063 Fragments arranged by size: 3063 1944 933 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2085 2327 2719 3055 3471 4376 5760 6028 Size: 453 242 392 336 416 905 1384 268 Cuts at: 6028 1632 Size: 1917 Fragments arranged by size: 1917 1384 905 453 416 392 336 268 242 AfeI AGC'GCT Cuts at: 3068 3068 Size: 6313 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 2457 5212 6189 1203 Size: 1254 2755 977 1327 Fragments arranged by size: 2755 1327 1254 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 2540 5042 2540 Size: 2502 3811 AleI CACnn'nnGTG Cuts at: 1166 2864 1166 Size: 1698 4615 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6313 AlwNI CAG_nnn'CTG Cuts at: 2206 2235 4056 5628 2206 Size: 29 1821 1572 2891 Fragments arranged by size: 2891 1821 1572 29 ApaLI G'TGCA_C Cuts at: 2293 4370 5526 2293 Size: 2077 1156 3080 Fragments arranged by size: 3080 2077 1156 ApoI r'AATT_y Cuts at: 1493 2517 2937 3105 3441 3622 3848 4000 6109 Size: 1024 420 168 336 181 226 152 2109 Cuts at: 6109 1493 Size: 1697 Fragments arranged by size: 2109 1697 1024 420 336 226 181 168 152 AseI AT'TA_AT Cuts at: 160 1032 4867 160 Size: 872 3835 1606 Fragments arranged by size: 3835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 2141 2830 3638 1870 Size: 73 198 689 808 4545 Fragments arranged by size: 4545 808 689 198 73 BamHI G'GATC_C Cuts at: 3021 3643 4010 3021 Size: 622 367 5324 Fragments arranged by size: 5324 622 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2676 3634 5089 618 Size: 325 217 627 889 958 1455 1842 Fragments arranged by size: 1842 1455 958 889 627 325 217 BanII G_rGCy'C Cuts at: 1391 2133 2166 2181 2355 2495 2747 2831 3335 Size: 742 33 15 174 140 252 84 504 Cuts at: 3335 3632 1391 Size: 297 4072 Fragments arranged by size: 4072 742 504 297 252 174 140 84 33 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4504 4538 1903 Size: 34 2567 34 3678 Fragments arranged by size: 3678 2567 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4205 5415 4205 Size: 1210 5103 BclI T'GATC_A Cuts at: 1548 1548 Size: 6313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4924 136 Size: 107 122 71 631 605 3252 1525 Fragments arranged by size: 3252 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6308 6308 Size: 6313 BlpI GC'TnA_GC Cuts at: 3730 3730 Size: 6313 Bme1580I G_kGCm'C Cuts at: 1790 2297 4374 5530 1790 Size: 507 2077 1156 2573 Fragments arranged by size: 2573 2077 1156 507 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3039 4044 5002 Size: 416 53 83 399 24 1622 1005 958 Cuts at: 5002 442 Size: 1753 Fragments arranged by size: 1753 1622 1005 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2152 2552 3140 3559 2152 Size: 400 588 419 4906 Fragments arranged by size: 4906 588 419 400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2547 3135 3219 3227 3387 4973 1239 Size: 1308 588 84 8 160 1586 2579 Fragments arranged by size: 2579 1586 1308 588 160 84 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2271 3568 4438 5303 5601 2271 Size: 1297 870 865 298 2983 Fragments arranged by size: 2983 1297 870 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2588 2695 2947 4976 882 Size: 617 1089 107 252 2029 2219 Fragments arranged by size: 2219 2029 1089 617 252 107 BsaAI yAC'GTr Cuts at: 493 1204 2460 3046 4104 493 Size: 711 1256 586 1058 2702 Fragments arranged by size: 2702 1256 1058 711 586 BsaBI GATnn'nnATC Cuts at: 6307 6307 Size: 6313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2105 3080 4120 4502 Size: 53 83 186 936 572 975 1040 382 Cuts at: 4502 5137 275 Size: 635 1451 Fragments arranged by size: 1451 1040 975 936 635 572 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2945 4746 5144 5418 5565 5976 2945 Size: 1801 398 274 147 411 3282 Fragments arranged by size: 3282 1801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3192 3222 1178 Size: 30 165 30 1789 30 4269 Fragments arranged by size: 4269 1789 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6313 BseYI C'CCAG_C Cuts at: 2441 3093 5516 6012 2441 Size: 652 2423 496 2742 Fragments arranged by size: 2742 2423 652 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3332 3406 1163 Size: 573 1596 74 4070 Fragments arranged by size: 4070 1596 573 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 2181 2297 2355 2495 2747 2831 3267 3335 Size: 48 116 58 140 252 84 436 68 Cuts at: 3335 4374 4459 5530 2133 Size: 1039 85 1071 2916 Fragments arranged by size: 2916 1071 1039 436 252 140 116 85 84 68 58 48 BsmI GAATG_Cn' Cuts at: 2717 2885 2900 2969 3824 3917 2717 Size: 168 15 69 855 93 5113 Fragments arranged by size: 5113 855 168 93 69 15 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2166 2181 2297 2355 2495 2747 Size: 399 343 33 15 116 58 140 252 Cuts at: 2747 2831 3267 3335 3632 4374 4459 5530 1391 Size: 84 436 68 297 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 436 399 343 297 252 140 116 85 84 68 58 33 15 BspEI T'CCGG_A Cuts at: 5976 5976 Size: 6313 BspHI T'CATG_A Cuts at: 4202 5932 4202 Size: 1730 4583 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2233 3668 844 Size: 1389 1435 3489 Fragments arranged by size: 3489 1435 1389 BsrBI CCG'CTC Cuts at: 1394 1823 4200 1394 Size: 429 2377 3507 Fragments arranged by size: 3507 2377 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2806 2974 3058 4808 4982 65 Size: 1183 1558 168 84 1750 174 1396 Fragments arranged by size: 1750 1558 1396 1183 174 168 84 BsrFI r'CCGG_y Cuts at: 1700 1810 4957 5144 1700 Size: 110 3147 187 2869 Fragments arranged by size: 3147 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 2159 4373 5385 2159 Size: 2214 1012 3087 Fragments arranged by size: 3087 2214 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6313 BstBI TT'CG_AA Cuts at: 3297 5117 3297 Size: 1820 4493 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstXI CCAn_nnnn'nTGG Cuts at: 3568 3568 Size: 6313 BstYI r'GATC_y Cuts at: 1972 2265 2853 3021 3273 3643 4010 4395 4412 Size: 293 588 168 252 370 367 385 17 Cuts at: 4412 5864 5950 5962 6308 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 588 385 370 367 346 293 252 168 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5761 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2894 487 Size: 683 56 1668 3906 Fragments arranged by size: 3906 1668 683 56 BtsI GCAGTG_nn' Cuts at: 2642 3829 4623 4643 2642 Size: 1187 794 20 4312 Fragments arranged by size: 4312 1187 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2704 2739 2788 2823 2872 2907 2956 Size: 35 2140 35 49 35 49 35 49 Cuts at: 2956 2991 3040 3075 529 Size: 35 49 35 3767 Fragments arranged by size: 3767 2140 49 49 49 49 35 35 35 35 35 35 DraI TTT'AAA Cuts at: 3617 3617 Size: 6313 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5320 809 Size: 507 4004 1802 Fragments arranged by size: 4004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2485 3760 4649 Size: 54 1425 50 275 18 655 1275 889 Cuts at: 4649 6278 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1275 889 655 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3447 4243 1353 Size: 2094 796 3423 Fragments arranged by size: 3423 2094 796 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4900 5274 5420 387 Size: 1064 138 3311 374 146 1280 Fragments arranged by size: 3311 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2131 2179 2353 2493 2745 2829 3333 2131 Size: 48 174 140 252 84 504 5111 Fragments arranged by size: 5111 504 252 174 140 84 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2327 2547 2719 3055 3135 3219 Size: 393 453 242 220 172 336 80 84 Cuts at: 3219 3227 3387 3471 4376 4973 5760 6028 1239 Size: 8 160 84 905 597 787 268 1524 Fragments arranged by size: 1524 905 787 597 453 393 336 268 242 220 172 160 84 84 80 8 EcoNI CCTnn'n_nnAGG Cuts at: 2821 2821 Size: 6313 EcoO109I rG'GnC_Cy Cuts at: 2787 3291 3556 2787 Size: 504 265 5544 Fragments arranged by size: 5544 504 265 EcoRI G'AATT_C Cuts at: 2517 2937 3105 3622 4000 2517 Size: 420 168 517 378 4830 Fragments arranged by size: 4830 517 420 378 168 EcoRV GAT'ATC Cuts at: 2383 2383 Size: 6313 FspI TGC'GCA Cuts at: 2634 4819 2634 Size: 2185 4128 HaeII r_GCGC'y Cuts at: 1678 3070 5460 1678 Size: 1392 2390 2531 Fragments arranged by size: 2531 2390 1392 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2526 2558 3479 Size: 32 135 32 503 32 419 32 921 Cuts at: 3479 3511 4954 4986 5028 5060 1373 Size: 32 1443 32 42 32 2626 Fragments arranged by size: 2626 1443 921 503 419 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3657 3903 669 Size: 988 281 1719 246 3079 Fragments arranged by size: 3079 1719 988 281 246 HindIII A'AGCT_T Cuts at: 748 3150 748 Size: 2402 3911 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KpnI G_GTAC'C Cuts at: 3638 3638 Size: 6313 MfeI C'AATT_G Cuts at: 3912 3912 Size: 6313 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5427 5611 1305 Size: 4122 184 2007 Fragments arranged by size: 4122 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2487 10 Size: 54 1425 343 655 3836 Fragments arranged by size: 3836 1425 655 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 2864 3570 4271 4630 4789 518 Size: 648 957 741 706 701 359 159 2042 Fragments arranged by size: 2042 957 741 706 701 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3729 4406 5554 5799 1606 Size: 2123 677 1148 245 2120 Fragments arranged by size: 2123 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5761 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 2441 3671 5216 1132 Size: 97 1212 1230 1545 2229 Fragments arranged by size: 2229 1545 1230 1212 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6313 PciI A'CATG_T Cuts at: 5212 5212 Size: 6313 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PmlI CAC'GTG Cuts at: 2460 2460 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6313 PsiI TTA'TAA Cuts at: 3883 3883 Size: 6313 PspXI vC'TCGA_Gb Cuts at: 2830 2830 Size: 6313 PstI C_TGCA'G Cuts at: 830 3665 830 Size: 2835 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 SacI G_AGCT'C Cuts at: 2133 2181 2355 2495 2747 2831 3335 2133 Size: 48 174 140 252 84 504 5111 Fragments arranged by size: 5111 504 252 174 140 84 48 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SfcI C'TryA_G Cuts at: 826 1045 3582 3661 4796 5477 5668 826 Size: 219 2537 79 1135 681 191 1471 Fragments arranged by size: 2537 1471 1135 681 219 191 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3640 1872 Size: 1768 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2286 2830 3547 4417 5318 5580 Size: 197 926 343 544 717 870 901 262 Cuts at: 5580 820 Size: 1553 Fragments arranged by size: 1553 926 901 870 717 544 343 262 197 SnaBI TAC'GTA Cuts at: 493 3046 4104 493 Size: 2553 1058 2702 Fragments arranged by size: 2702 2553 1058 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 2441 3671 1132 Size: 1309 1230 3774 Fragments arranged by size: 3774 1309 1230 SspI AAT'ATT Cuts at: 5 52 4237 5 Size: 47 4185 2081 Fragments arranged by size: 4185 2081 47 StuI AGG'CCT Cuts at: 1517 3040 1517 Size: 1523 4790 StyI C'CwwG_G Cuts at: 513 1065 3752 513 Size: 552 2687 3074 Fragments arranged by size: 3074 2687 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3107 3560 4340 4357 4510 4695 5140 Size: 1255 1527 453 780 17 153 185 445 Cuts at: 5140 325 Size: 1498 Fragments arranged by size: 1527 1498 1255 780 453 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4559 6271 96 Size: 274 80 33 51 1443 2582 1712 138 Fragments arranged by size: 2582 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3192 3224 6273 6305 3192 Size: 32 3049 32 3200 Fragments arranged by size: 3200 3049 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2104 1316 Size: 788 5525 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XhoI C'TCGA_G Cuts at: 1943 2830 1943 Size: 887 5426 XmaI C'CCGG_G Cuts at: 1870 3638 1870 Size: 1768 4545 XmnI GAAnn'nnTTC Cuts at: 2564 2732 2816 2900 3068 3152 3320 4442 2564 Size: 168 84 84 168 84 168 1122 4435 Fragments arranged by size: 4435 1122 168 168 168 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2106 3081 4121 276 Size: 53 83 186 936 572 975 1040 2468 Fragments arranged by size: 2468 1040 975 936 572 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI