Restriction map for
pFN21AA0612
(Circular) (Six-base) MAPSORT of: pFN21AA0612.seq Check: 2442 from: 1 to: 10096
pFN21AA0612 10096 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3007 4597 4963 3007
Size: 1590 366 8140
Fragments arranged by size:
8140 1590 366
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7906 278
Size: 53 83 186 936 6370 2468
Fragments arranged by size:
6370 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 7439 1937
Size: 1362 4140 4594
Fragments arranged by size:
4594 4140 1362
Acc65I G'GTAC_C
Cuts at: 7417 7417
Size: 10096
AclI AA'CG_TT
Cuts at: 1563 8223 8596 1563
Size: 6660 373 3063
Fragments arranged by size:
6660 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 2354 2510 3158 3276 5118 6824 8159
Size: 624 98 156 648 118 1842 1706 1335
Cuts at: 8159 9543 9811 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1842 1706 1384 1335 648 624 268
156 118 98
AfeI AGC'GCT
Cuts at: 2411 2411
Size: 10096
AflII C'TTAA_G
Cuts at: 820 1017 2423 820
Size: 197 1406 8493
Fragments arranged by size:
8493 1406 197
AflIII A'CryG_T
Cuts at: 1203 8995 9972 1203
Size: 7792 977 1327
Fragments arranged by size:
7792 1327 977
AgeI A'CCGG_T
Cuts at: 8927 8927
Size: 10096
AhdI GACnn_n'nnGTC
Cuts at: 2091 5497 6739 8825 2091
Size: 3406 1242 2086 3362
Fragments arranged by size:
3406 3362 2086 1242
AleI CACnn'nnGTG
Cuts at: 1166 4529 1166
Size: 3363 6733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2430 4737 1184
Size: 1246 2307 6543
Fragments arranged by size:
6543 2307 1246
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4038 4070 4038
Size: 32 10064
AlwNI CAG_nnn'CTG
Cuts at: 2443 2842 3055 3103 3175 3292 4043 4327 4408
Size: 399 213 48 72 117 751 284 81
Cuts at: 4408 4969 5150 5501 5773 6381 6419 6802 6928
Size: 561 181 351 272 608 38 383 126
Cuts at: 6928 7839 9411 2443
Size: 911 1572 3128
Fragments arranged by size:
3128 1572 911 751 608 561 399 383
351 284 272 213 181 126 117 81
72 48 38
ApaI G_GGCC'C
Cuts at: 2392 2545 2717 2758 3416 4386 4686 4797 5455
Size: 153 172 41 658 970 300 111 658
Cuts at: 5455 6135 6158 6197 6239 6608 7310 2392
Size: 680 23 39 42 369 702 5178
Fragments arranged by size:
5178 970 702 680 658 658 369 300
172 153 111 42 41 39 23
ApaLI G'TGCA_C
Cuts at: 8153 9309 8153
Size: 1156 8940
ApoI r'AATT_y
Cuts at: 1493 7405 7631 7783 9892 1493
Size: 5912 226 152 2109 1697
Fragments arranged by size:
5912 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8650 160
Size: 872 7618 1606
Fragments arranged by size:
7618 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10096
AvaI C'yCGr_G
Cuts at: 1870 1943 2710 2750 2758 3321 3409 4461 5079
Size: 73 767 40 8 563 88 1052 618
Cuts at: 5079 5886 6552 7421 1870
Size: 807 666 869 4545
Fragments arranged by size:
4545 1052 869 807 767 666 618 563
88 73 40 8
BamHI G'GATC_C
Cuts at: 3786 7426 7793 3786
Size: 3640 367 6089
Fragments arranged by size:
6089 3640 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2740 4339 4457 4474 4531
Size: 325 217 627 953 1599 118 17 57
Cuts at: 4531 5309 5461 7417 8872 618
Size: 778 152 1956 1455 1842
Fragments arranged by size:
1956 1842 1599 1455 953 778 627 325
217 152 118 57 17
BanII G_rGCy'C
Cuts at: 1391 2210 2392 2545 2717 2758 2856 3336 3390
Size: 819 182 153 172 41 98 480 54
Cuts at: 3390 3416 3545 3930 4152 4163 4386 4686 4797
Size: 26 129 385 222 11 223 300 111
Cuts at: 4797 5455 6056 6135 6158 6197 6239 6608 6671
Size: 658 601 79 23 39 42 369 63
Cuts at: 6671 6974 7310 7415 1391
Size: 303 336 105 4072
Fragments arranged by size:
4072 819 658 601 480 385 369 336
303 300 223 222 182 172 153 129
111 105 98 79 63 54 42 41
39 26 23 11
BbeI G_GCGC'C
Cuts at: 2744 4343 5465 2744
Size: 1599 1122 7375
Fragments arranged by size:
7375 1599 1122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3561 3787 5956 6012 928
Size: 971 1662 226 2169 56 5012
Fragments arranged by size:
5012 2169 1662 971 226 56
BbvCI CC'TCA_GC
Cuts at: 2384 2444 3041 5507 6891 2384
Size: 60 597 2466 1384 5589
Fragments arranged by size:
5589 2466 1384 597 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4172 4206 4961 4995 5070 5104 5387
Size: 34 2235 34 755 34 75 34 283
Cuts at: 5387 5421 8287 8321 1903
Size: 34 2866 34 3678
Fragments arranged by size:
3678 2866 2235 755 283 75 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7988 9198 7988
Size: 1210 8886
BclI T'GATC_A
Cuts at: 1548 4812 1548
Size: 3264 6832
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2074 2510 2704
Size: 107 122 71 631 605 402 436 194
Cuts at: 2704 3623 3945 5423 5459 6234 6436 6864 8707
Size: 919 322 1478 36 775 202 428 1843
Cuts at: 8707 136
Size: 1525
Fragments arranged by size:
1843 1525 1478 919 775 631 605 436
428 402 322 202 194 122 107 71
36
BglII A'GATC_T
Cuts at: 5757 10091 5757
Size: 4334 5762
BlpI GC'TnA_GC
Cuts at: 2855 3203 4665 7513 2855
Size: 348 1462 2848 5438
Fragments arranged by size:
5438 2848 1462 348
Bme1580I G_kGCm'C
Cuts at: 1790 2065 2392 2545 2717 2758 3416 4386 4462
Size: 275 327 153 172 41 658 970 76
Cuts at: 4462 4479 4536 4586 4686 4797 5312 5455 6135
Size: 17 57 50 100 111 515 143 680
Cuts at: 6135 6158 6197 6239 6413 6608 7310 8157 9313
Size: 23 39 42 174 195 702 847 1156
Cuts at: 9313 1790
Size: 2573
Fragments arranged by size:
2573 1156 970 847 702 680 658 515
327 275 195 174 172 153 143 111
100 76 57 50 42 41 39 23
17
BmgBI CAC'GTC
Cuts at: 1318 7180 1318
Size: 5862 4234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2578 3606 4593
Size: 416 53 83 399 24 1161 1028 987
Cuts at: 4593 4618 4823 6161 7023 7827 8785 442
Size: 25 205 1338 862 804 958 1753
Fragments arranged by size:
1753 1338 1161 1028 987 958 862 804
416 399 205 83 53 25 24
BmtI G_CTAG'C
Cuts at: 1056 6219 1056
Size: 5163 4933
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2998 3094 3652 4936 6565 2998
Size: 96 558 1284 1629 6529
Fragments arranged by size:
6529 1629 1284 558 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2064 2073 2194 2396 2552 2708 2906 3137
Size: 825 9 121 202 156 156 198 231
Cuts at: 3137 3146 3197 3314 3374 3746 3993 4379 4730
Size: 9 51 117 60 372 247 386 351
Cuts at: 4730 5156 5172 5190 5250 5413 5771 5795 5968
Size: 426 16 18 60 163 358 24 173
Cuts at: 5968 6012 6072 6290 6411 6786 6917 7322 8756
Size: 44 60 218 121 375 131 405 1434
Cuts at: 8756 1239
Size: 2579
Fragments arranged by size:
2579 1434 825 426 405 386 375 372
358 351 247 231 218 202 198 173
163 156 156 131 121 121 117 60
60 60 51 44 24 18 16 9
9
Bpu10I CC'TnA_GC
Cuts at: 2384 2444 3041 4036 4269 5507 6891 6973 7089
Size: 60 597 995 233 1238 1384 82 116
Cuts at: 7089 2384
Size: 5391
Fragments arranged by size:
5391 1384 1238 995 597 233 116 82
60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4208 5181 6986 8221 9086 9384 4208
Size: 973 1805 1235 865 298 4920
Fragments arranged by size:
4920 1805 1235 973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5148 5484 5883 6557 8759 882
Size: 617 3649 336 399 674 2202 2219
Fragments arranged by size:
3649 2219 2202 674 617 399 336
BsaAI yAC'GTr
Cuts at: 493 1204 2596 7887 493
Size: 711 1392 5291 2702
Fragments arranged by size:
5291 2702 1392 711
BsaBI GATnn'nnATC
Cuts at: 4811 10090 4811
Size: 5279 4817
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2741 4340 5462 7903
Size: 53 83 186 936 1208 1599 1122 2441
Cuts at: 7903 8285 8920 275
Size: 382 635 1451
Fragments arranged by size:
2441 1599 1451 1208 1122 936 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3231 4168 6566 8529 8927 9201 9348 9759 3231
Size: 937 2398 1963 398 274 147 411 3568
Fragments arranged by size:
3568 2398 1963 937 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 3372 3402 3725
Size: 30 165 30 789 30 1150 30 323
Cuts at: 3725 3755 5384 5414 5849 5879 7003 7033 1178
Size: 30 1629 30 435 30 1124 30 4241
Fragments arranged by size:
4241 1629 1150 1124 789 435 323 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 2192 2549 2936 2990 3239 3634 3734
Size: 944 62 357 387 54 249 395 100
Cuts at: 3734 3792 4142 4145 5157 5300 5333 5502 5636
Size: 58 350 3 1012 143 33 169 134
Cuts at: 5636 5639 5642 5645 5648 5657 5768 5840 5843
Size: 3 3 3 3 9 111 72 3
Cuts at: 5843 5858 5867 5870 5873 6055 6060 6679 7011
Size: 15 9 3 3 182 5 619 332
Cuts at: 7011 7390 7393 1186
Size: 379 3 3889
Fragments arranged by size:
3889 1012 944 619 395 387 379 357
350 332 249 182 169 143 134 111
100 72 62 58 54 33 15 9
9 5 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2258 2628 3159 3495 3751 4478 4698 4747 4956
Size: 370 531 336 256 727 220 49 209
Cuts at: 4956 5159 5291 5305 5454 5647 5718 5917 5923
Size: 203 132 14 149 193 71 199 6
Cuts at: 5923 6232 6481 6914 7068 9299 9795 2258
Size: 309 249 433 154 2231 496 2559
Fragments arranged by size:
2559 2231 727 531 496 433 370 336
309 256 249 220 209 203 199 193
154 149 132 71 49 14 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2136 2855 3305 3389 3541 4799 1163
Size: 573 400 719 450 84 152 1258 6460
Fragments arranged by size:
6460 1258 719 573 450 400 152 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2611 5581 7546 8307 9335 1540
Size: 275 186 610 2970 1965 761 1028 2301
Fragments arranged by size:
2970 2301 1965 1028 761 610 275 186
BsiHKAI G_wGCw'C
Cuts at: 2210 2856 3336 3390 3930 4152 5215 5449 6254
Size: 646 480 54 540 222 1063 234 805
Cuts at: 6254 8157 8242 9313 2210
Size: 1903 85 1071 2993
Fragments arranged by size:
2993 1903 1071 1063 805 646 540 480
234 222 85 54
BsmI GAATG_Cn'
Cuts at: 2965 3037 7607 7700 2965
Size: 72 4570 93 5361
Fragments arranged by size:
5361 4570 93 72
BsmBI CGTCTCn'nnnn_
Cuts at: 2193 2675 2193
Size: 482 9614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2065 2210 2392 2545 2717 2758 2856
Size: 399 275 145 182 153 172 41 98
Cuts at: 2856 3336 3390 3416 3545 3930 4152 4163 4386
Size: 480 54 26 129 385 222 11 223
Cuts at: 4386 4462 4479 4536 4586 4686 4797 5215 5312
Size: 76 17 57 50 100 111 418 97
Cuts at: 5312 5449 5455 6056 6135 6158 6197 6239 6254
Size: 137 6 601 79 23 39 42 15
Cuts at: 6254 6413 6608 6671 6974 7310 7415 8157 8242
Size: 159 195 63 303 336 105 742 85
Cuts at: 8242 9313 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 601 480 418 399 385
336 303 275 223 222 195 182 172
159 153 145 137 129 111 105 100
98 97 85 79 76 63 57 54
50 42 41 39 26 23 17 15
11 6
BspEI T'CCGG_A
Cuts at: 3231 9759 3231
Size: 6528 3568
BspHI T'CATG_A
Cuts at: 7985 9715 7985
Size: 1730 8366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3007 4380 4597 4963 5668 5917 7451 844
Size: 2163 1373 217 366 705 249 1534 3489
Fragments arranged by size:
3489 2163 1534 1373 705 366 249 217
BsrBI CCG'CTC
Cuts at: 1394 1823 2692 6508 7038 7983 1394
Size: 429 869 3816 530 945 3507
Fragments arranged by size:
3816 3507 945 869 530 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2517 5912 8591 8765 65
Size: 1183 1269 3395 2679 174 1396
Fragments arranged by size:
3395 2679 1396 1269 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6228 8740 8927 1700
Size: 110 4418 2512 187 2869
Fragments arranged by size:
4418 2869 2512 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10096
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10096
BssSI C'ACGA_G
Cuts at: 5255 5567 8156 9168 5255
Size: 312 2589 1012 6183
Fragments arranged by size:
6183 2589 1012 312
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2833 4969 5219 794
Size: 2039 2136 250 5671
Fragments arranged by size:
5671 2136 2039 250
BstBI TT'CG_AA
Cuts at: 8900 8900
Size: 10096
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10096
BstXI CCAn_nnnn'nTGG
Cuts at: 2218 2530 6143 6307 7300 2218
Size: 312 3613 164 993 5014
Fragments arranged by size:
5014 3613 993 312 164
BstYI r'GATC_y
Cuts at: 1972 3421 3786 4806 5757 5769 6091 6708 6798
Size: 1449 365 1020 951 12 322 617 90
Cuts at: 6798 7426 7793 8178 8195 9647 9733 9745 10091
Size: 628 367 385 17 1452 86 12 346
Cuts at: 10091 1972
Size: 1977
Fragments arranged by size:
1977 1452 1449 1020 951 628 617 385
367 365 346 322 90 86 17 12
12
Bsu36I CC'TnA_GG
Cuts at: 2567 3693 4125 6632 7317 2567
Size: 1126 432 2507 685 5346
Fragments arranged by size:
5346 2507 1126 685 432
BtgI C'CryG_G
Cuts at: 513 1065 2004 2027 2049 2057 2217 4926 5011
Size: 552 939 23 22 8 160 2709 85
Cuts at: 5011 5575 6256 7128 513
Size: 564 681 872 3481
Fragments arranged by size:
3481 2709 939 872 681 564 552 160
85 23 22 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2113 2929 5763 487
Size: 683 56 887 816 2834 4820
Fragments arranged by size:
4820 2834 887 816 683 56
BtsI GCAGTG_nn'
Cuts at: 2063 2119 4105 4433 4942 5307 6103 6526 6642
Size: 56 1986 328 509 365 796 423 116
Cuts at: 6642 7612 8406 8426 2063
Size: 970 794 20 3733
Fragments arranged by size:
3733 1986 970 796 794 509 423 365
328 116 56 20
ClaI AT'CG_AT
Cuts at: 1554 4839 1554
Size: 3285 6811
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10061
DraI TTT'AAA
Cuts at: 7400 7400
Size: 10096
DraIII CAC_nnn'GTG
Cuts at: 4243 4243
Size: 10096
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9103 809
Size: 507 7787 1802
Fragments arranged by size:
7787 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3937 4017 5064
Size: 54 1425 50 275 18 2107 80 1047
Cuts at: 5064 5382 5578 5956 6259 6282 7543 8432 10061
Size: 318 196 378 303 23 1261 889 1629
Cuts at: 10061 8
Size: 43
Fragments arranged by size:
2107 1629 1425 1261 1047 889 378 318
303 275 196 80 54 50 43 23
18
EagI C'GGCC_G
Cuts at: 1537 1812 5578 7543 1537
Size: 275 3766 1965 4090
Fragments arranged by size:
4090 3766 1965 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 2466 2486 4572 5607 5613 5634 5829
Size: 1091 22 20 2086 1035 6 21 195
Cuts at: 5829 5835 6608 6768 7378 8026 1353
Size: 6 773 160 610 648 3423
Fragments arranged by size:
3423 2086 1091 1035 773 648 610 195
160 22 21 20 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3389 5039 6613 8683 9057 9203
Size: 1064 138 1800 1650 1574 2070 374 146
Cuts at: 9203 387
Size: 1280
Fragments arranged by size:
2070 1800 1650 1574 1280 1064 374 146
138
EcoICRI GAG'CTC
Cuts at: 2208 2854 3334 3388 3928 4150 2208
Size: 646 480 54 540 222 8154
Fragments arranged by size:
8154 646 540 480 222 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2073 2194 2256 2354 2396 2510
Size: 393 432 9 121 62 98 42 114
Cuts at: 2510 2552 2708 2906 3137 3146 3158 3197 3276
Size: 42 156 198 231 9 12 39 79
Cuts at: 3276 3314 3374 3746 3993 4379 4730 5118 5156
Size: 38 60 372 247 386 351 388 38
Cuts at: 5156 5172 5190 5250 5413 5771 5795 5968 6012
Size: 16 18 60 163 358 24 173 44
Cuts at: 6012 6072 6290 6411 6786 6824 6917 7322 8159
Size: 60 218 121 375 38 93 405 837
Cuts at: 8159 8756 9543 9811 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 837 787 597 432 405 393 388
386 375 372 358 351 268 247 231
218 198 173 163 156 121 121 114
98 93 79 62 60 60 60 44
42 42 39 38 38 38 24 18
16 12 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2299 2426 2888 2972 3380 7320 2299
Size: 127 462 84 408 3940 5075
Fragments arranged by size:
5075 3940 462 408 127 84
EcoO109I rG'GnC_Cy
Cuts at: 2038 2388 2389 2541 2579 2714 2754 2755 3100
Size: 350 1 152 38 135 40 1 345
Cuts at: 3100 3413 3601 3830 3946 4040 4174 4324 4331
Size: 313 188 229 116 94 134 150 7
Cuts at: 4331 4383 4405 4682 4683 4794 5147 5250 5260
Size: 52 22 277 1 111 353 103 10
Cuts at: 5260 5356 6132 6155 6193 6194 6236 6437 6458
Size: 96 776 23 38 1 42 201 21
Cuts at: 6458 6604 6605 7061 7082 7279 7306 7307 2038
Size: 146 1 456 21 197 27 1 4827
Fragments arranged by size:
4827 776 456 353 350 345 313 277
229 201 197 188 152 150 146 135
134 116 111 103 96 94 52 42
40 38 38 27 23 22 21 21
10 7 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 7405 7783 7405
Size: 378 9718
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 3872 5827 6794 2473
Size: 1399 1955 967 5775
Fragments arranged by size:
5775 1955 1399 967
FspI TGC'GCA
Cuts at: 2669 4997 8602 2669
Size: 2328 3605 4163
Fragments arranged by size:
4163 3605 2328
FspAI rTGC'GCAy
Cuts at: 4997 4997
Size: 10096
HaeII r_GCGC'y
Cuts at: 1678 2413 2530 2744 3556 4343 5126 5465 6269
Size: 735 117 214 812 787 783 339 804
Cuts at: 6269 9243 1678
Size: 2974 2531
Fragments arranged by size:
2974 2531 812 804 787 783 735 339
214 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 2192 2224 3725
Size: 32 135 32 505 32 83 32 1501
Cuts at: 3725 3757 3771 3803 3962 3994 5147 5179 5483
Size: 32 14 32 159 32 1153 32 304
Cuts at: 5483 5515 5849 5881 6725 6757 8737 8769 8811
Size: 32 334 32 844 32 1980 32 42
Cuts at: 8811 8843 1373
Size: 32 2626
Fragments arranged by size:
2626 1980 1501 1153 844 505 334 304
159 135 83 42 32 32 32 32
32 32 32 32 32 32 32 32
32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4503 7440 7686 669
Size: 988 281 2565 2937 246 3079
Fragments arranged by size:
3079 2937 2565 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10096
HpaI GTT'AAC
Cuts at: 7686 7686
Size: 10096
KasI G'GCGC_C
Cuts at: 2740 4339 5461 2740
Size: 1599 1122 7375
Fragments arranged by size:
7375 1599 1122
KpnI G_GTAC'C
Cuts at: 7421 7421
Size: 10096
MfeI C'AATT_G
Cuts at: 7695 7695
Size: 10096
MluI A'CGCG_T
Cuts at: 9972 9972
Size: 10096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3663 3960 4881 7035 7317 9210 9394 1305
Size: 2358 297 921 2154 282 1893 184 2007
Fragments arranged by size:
2358 2154 2007 1893 921 297 282 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5384 5958 6284 10
Size: 54 1425 343 3552 574 326 3822
Fragments arranged by size:
3822 3552 1425 574 343 326 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4299 4529 4913 7220 8054 8413 8572
Size: 648 3133 230 384 2307 834 359 159
Cuts at: 8572 518
Size: 2042
Fragments arranged by size:
3133 2307 2042 834 648 384 359 230
159
MspA1I CmG'CkG
Cuts at: 1606 2632 2677 2842 2938 3163 3175 3289 3755
Size: 1026 45 165 96 225 12 114 466
Cuts at: 3755 3862 3869 4231 4486 4717 4960 4966 6288
Size: 107 7 362 255 231 243 6 1322
Cuts at: 6288 6357 6937 7512 8189 9337 9582 1606
Size: 69 580 575 677 1148 245 2120
Fragments arranged by size:
2120 1322 1148 1026 677 580 575 466
362 255 245 243 231 225 165 114
107 96 69 45 12 7 6
NaeI GCC'GGC
Cuts at: 6230 6230
Size: 10096
NarI GG'CG_CC
Cuts at: 2741 4340 5462 2741
Size: 1599 1122 7375
Fragments arranged by size:
7375 1599 1122
NcoI C'CATG_G
Cuts at: 513 1065 2004 2049 2057 7128 513
Size: 552 939 45 8 5071 3481
Fragments arranged by size:
5071 3481 939 552 45 8
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10096
NgoMIV G'CCGG_C
Cuts at: 6228 6228
Size: 10096
NheI G'CTAG_C
Cuts at: 1052 6215 1052
Size: 5163 4933
NotI GC'GGCC_GC
Cuts at: 7543 7543
Size: 10096
NspI r_CATG'y
Cuts at: 1132 1229 3148 6013 7454 8999 1132
Size: 97 1919 2865 1441 1545 2229
Fragments arranged by size:
2865 2229 1919 1545 1441 97
PasI CC'CwG_GG
Cuts at: 1348 2392 3548 4327 4686 4802 5144 5344 5440
Size: 1044 1156 779 359 116 342 200 96
Cuts at: 5440 5495 5514 6432 7310 1348
Size: 55 19 918 878 4134
Fragments arranged by size:
4134 1156 1044 918 878 779 359 342
200 116 96 55 19
PciI A'CATG_T
Cuts at: 8995 8995
Size: 10096
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2033 3055 4243 5150 5501 7129 1108
Size: 925 1022 1188 907 351 1628 4075
Fragments arranged by size:
4075 1628 1188 1022 925 907 351
PfoI T'CCnGG_A
Cuts at: 5206 5604 5654 5772 5820 6242 7299 5206
Size: 398 50 118 48 422 1057 8003
Fragments arranged by size:
8003 1057 422 398 118 50 48
PmeI GTTT'AAAC
Cuts at: 7400 7400
Size: 10096
PmlI CAC'GTG
Cuts at: 2596 2596
Size: 10096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8165 8197 9678 9710 8165
Size: 32 1481 32 8551
Fragments arranged by size:
8551 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2038 2579 3601 3830 5250 5260 5356 2038
Size: 541 1022 229 1420 10 96 6778
Fragments arranged by size:
6778 1420 1022 541 229 96 10
PshAI GACnn'nnGTC
Cuts at: 1613 5937 1613
Size: 4324 5772
PsiI TTA'TAA
Cuts at: 7666 7666
Size: 10096
PspOMI G'GGCC_C
Cuts at: 2388 2541 2713 2754 3412 4382 4682 4793 5451
Size: 153 172 41 658 970 300 111 658
Cuts at: 5451 6131 6154 6193 6235 6604 7306 2388
Size: 680 23 39 42 369 702 5178
Fragments arranged by size:
5178 970 702 680 658 658 369 300
172 153 111 42 41 39 23
PstI C_TGCA'G
Cuts at: 830 2157 2556 2652 2937 3108 3243 4734 4965
Size: 1327 399 96 285 171 135 1491 231
Cuts at: 4965 4974 5410 6110 6649 7448 830
Size: 9 436 700 539 799 3478
Fragments arranged by size:
3478 1491 1327 799 700 539 436 399
285 231 171 135 96 9
PvuI CG_AT'CG
Cuts at: 2001 2611 2001
Size: 610 9486
PvuII CAG'CTG
Cuts at: 2842 2938 3175 3289 3755 3869 4231 4486 4960
Size: 96 237 114 466 114 362 255 474
Cuts at: 4960 4966 6288 2842
Size: 6 1322 6650
Fragments arranged by size:
6650 1322 474 466 362 255 237 114
114 96 6
SacI G_AGCT'C
Cuts at: 2210 2856 3336 3390 3930 4152 2210
Size: 646 480 54 540 222 8154
Fragments arranged by size:
8154 646 540 480 222 54
SalI G'TCGA_C
Cuts at: 1936 7438 1936
Size: 5502 4594
SanDI GG'GwC_CC
Cuts at: 2038 2579 3601 3830 5250 2038
Size: 541 1022 229 1420 6884
Fragments arranged by size:
6884 1420 1022 541 229
SapI GCTCTTCn'nnn_
Cuts at: 2444 6768 2444
Size: 4324 5772
SbfI CC_TGCA'GG
Cuts at: 7448 7448
Size: 10096
ScaI AGT'ACT
Cuts at: 8344 10056 8344
Size: 1712 8384
SfcI C'TryA_G
Cuts at: 826 1045 2153 2552 2648 2933 3104 3239 4730
Size: 219 1108 399 96 285 171 135 1491
Cuts at: 4730 4961 4970 5406 5797 6106 6420 6645 7444
Size: 231 9 436 391 309 314 225 799
Cuts at: 7444 8579 9260 9451 826
Size: 1135 681 191 1471
Fragments arranged by size:
1491 1471 1135 1108 799 681 436 399
391 314 309 285 231 225 219 191
171 135 96 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3945 5423 6234 6436 3945
Size: 1478 811 202 7605
Fragments arranged by size:
7605 1478 811 202
SfoI GGC'GCC
Cuts at: 2742 4341 5463 2742
Size: 1599 1122 7375
Fragments arranged by size:
7375 1599 1122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10096
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10096
SmaI CCC'GGG
Cuts at: 1872 2712 2752 2760 3323 3411 4463 6554 7423
Size: 840 40 8 563 88 1052 2091 869
Cuts at: 7423 1872
Size: 4545
Fragments arranged by size:
4545 2091 1052 869 840 563 88 40
8
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2423 4223 5196 6965 8200 9101
Size: 197 926 480 1800 973 1769 1235 901
Cuts at: 9101 9363 820
Size: 262 1553
Fragments arranged by size:
1800 1769 1553 1235 973 926 901 480
262 197
SnaBI TAC'GTA
Cuts at: 493 7887 493
Size: 7394 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10096
SphI G_CATG'C
Cuts at: 1132 7454 1132
Size: 6322 3774
SrfI GCCC'GGGC
Cuts at: 4463 4463
Size: 10096
SspI AAT'ATT
Cuts at: 5 52 8020 5
Size: 47 7968 2081
Fragments arranged by size:
7968 2081 47
StuI AGG'CCT
Cuts at: 1517 5417 6140 6404 1517
Size: 3900 723 264 5209
Fragments arranged by size:
5209 3900 723 264
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2057 3303 3417 3454 4347
Size: 552 939 45 8 1246 114 37 893
Cuts at: 4347 4678 5188 5482 6537 7128 7535 513
Size: 331 510 294 1055 591 407 3074
Fragments arranged by size:
3074 1246 1055 939 893 591 552 510
407 331 294 114 45 37 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4360 4445 4519 4939 5118 5336 5453
Size: 1255 2780 85 74 420 179 218 117
Cuts at: 5453 8123 8140 8293 8478 8923 325
Size: 2670 17 153 185 445 1498
Fragments arranged by size:
2780 2670 1498 1255 445 420 218 185
179 153 117 85 74 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3876 4939 6993
Size: 274 80 33 51 1443 1899 1063 2054
Cuts at: 6993 8342 10054 96
Size: 1349 1712 138
Fragments arranged by size:
2054 1899 1712 1443 1349 1063 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3372 3404 3641 3673 5384 5416 10056 10088 3372
Size: 32 237 32 1711 32 4640 32 3380
Fragments arranged by size:
4640 3380 1711 237 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3737 1316
Size: 2421 7675
XbaI T'CTAG_A
Cuts at: 7432 7432
Size: 10096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4354 4631 5288 6848 7177 4354
Size: 277 657 1560 329 7273
Fragments arranged by size:
7273 1560 657 329 277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10096
XmaI C'CCGG_G
Cuts at: 1870 2710 2750 2758 3321 3409 4461 6552 7421
Size: 840 40 8 563 88 1052 2091 869
Cuts at: 7421 1870
Size: 4545
Fragments arranged by size:
4545 2091 1052 869 840 563 88 40
8
XmnI GAAnn'nnTTC
Cuts at: 6777 8225 6777
Size: 1448 8648
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7904 276
Size: 53 83 186 936 6370 2468
Fragments arranged by size:
6370 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI
NruI NsiI PacI PspXI PsrI RsrII SacII SexAI
SwaI