(Circular) (Six-base) MAPSORT of: pFN21AA0605.seq Check: 9619 from: 1 to: 7558 pFN21AA0605 7558 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3749 3923 4439 3749 Size: 174 516 6868 Fragments arranged by size: 6868 516 174 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4638 5368 278 Size: 53 83 186 936 3102 730 2468 Fragments arranged by size: 3102 2468 936 730 186 83 53 AccI GT'mk_AC Cuts at: 1937 4901 1937 Size: 2964 4594 Acc65I G'GTAC_C Cuts at: 2292 4879 2292 Size: 2587 4971 AclI AA'CG_TT Cuts at: 1563 3603 5685 6058 1563 Size: 2040 2082 373 3063 Fragments arranged by size: 3063 2082 2040 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5621 7005 7273 1632 Size: 3989 1384 268 1917 Fragments arranged by size: 3989 1917 1384 268 AfeI AGC'GCT Cuts at: 3052 3052 Size: 7558 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7361 AflIII A'CryG_T Cuts at: 1203 2373 3696 4287 4547 6457 7434 1203 Size: 1170 1323 591 260 1910 977 1327 Fragments arranged by size: 1910 1327 1323 1170 977 591 260 AgeI A'CCGG_T Cuts at: 3702 6389 3702 Size: 2687 4871 AhdI GACnn_n'nnGTC Cuts at: 2077 6287 2077 Size: 4210 3348 AleI CACnn'nnGTG Cuts at: 1166 2648 4368 1166 Size: 1482 1720 4356 Fragments arranged by size: 4356 1720 1482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3632 1184 Size: 2448 5110 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3889 3921 3889 Size: 32 7526 AlwNI CAG_nnn'CTG Cuts at: 2271 2932 3067 3391 3404 3813 4587 5301 6873 Size: 661 135 324 13 409 774 714 1572 Cuts at: 6873 2271 Size: 2956 Fragments arranged by size: 2956 1572 774 714 661 409 324 135 13 ApaI G_GGCC'C Cuts at: 2258 2919 3194 3258 4065 4208 2258 Size: 661 275 64 807 143 5608 Fragments arranged by size: 5608 807 661 275 143 64 ApaLI G'TGCA_C Cuts at: 4093 4597 5615 6771 4093 Size: 504 1018 1156 4880 Fragments arranged by size: 4880 1156 1018 504 ApoI r'AATT_y Cuts at: 1493 4867 5093 5245 7354 1493 Size: 3374 226 152 2109 1697 Fragments arranged by size: 3374 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6112 160 Size: 872 5080 1606 Fragments arranged by size: 5080 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7558 AvaI C'yCGr_G Cuts at: 1870 1943 2251 2258 2721 3169 3833 4025 4508 Size: 73 308 7 463 448 664 192 483 Cuts at: 4508 4535 4883 1870 Size: 27 348 4545 Fragments arranged by size: 4545 664 483 463 448 348 308 192 73 27 7 BamHI G'GATC_C Cuts at: 4888 5255 4888 Size: 367 7191 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2292 2717 3458 4766 Size: 325 217 627 342 163 425 741 1308 Cuts at: 4766 4879 6334 618 Size: 113 1455 1842 Fragments arranged by size: 1842 1455 1308 741 627 425 342 325 217 163 113 BanII G_rGCy'C Cuts at: 1391 2258 2565 2919 3194 3258 3473 3729 4065 Size: 867 307 354 275 64 215 256 336 Cuts at: 4065 4208 4521 4737 4877 1391 Size: 143 313 216 140 4072 Fragments arranged by size: 4072 867 354 336 313 307 275 256 216 215 143 140 64 BbeI G_GCGC'C Cuts at: 3462 3462 Size: 7558 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6587 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2523 2557 3551 3585 4079 4113 4534 Size: 34 586 34 994 34 494 34 421 Cuts at: 4534 4568 5749 5783 1903 Size: 34 1181 34 3678 Fragments arranged by size: 3678 1181 994 586 494 421 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5450 6660 5450 Size: 1210 6348 BclI T'GATC_A Cuts at: 1548 1548 Size: 7558 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3171 6169 136 Size: 107 122 71 631 605 1499 2998 1525 Fragments arranged by size: 2998 1525 1499 631 605 122 107 71 BglII A'GATC_T Cuts at: 7553 7553 Size: 7558 BlpI GC'TnA_GC Cuts at: 3554 4975 3554 Size: 1421 6137 Bme1580I G_kGCm'C Cuts at: 1790 2132 2258 2282 2722 2919 3194 3258 4065 Size: 342 126 24 440 197 275 64 807 Cuts at: 4065 4097 4208 4601 4780 4809 5619 6775 1790 Size: 32 111 393 179 29 810 1156 2573 Fragments arranged by size: 2573 1156 810 807 440 393 342 275 197 179 126 111 64 32 29 24 BmgBI CAC'GTC Cuts at: 1318 2283 2761 1318 Size: 965 478 6115 Fragments arranged by size: 6115 965 478 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4060 4249 4324 Size: 416 53 83 399 24 2643 189 75 Cuts at: 4324 4420 5289 6247 442 Size: 96 869 958 1753 Fragments arranged by size: 2643 1753 958 869 416 399 189 96 83 75 53 24 BmtI G_CTAG'C Cuts at: 1056 2768 1056 Size: 1712 5846 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3379 3379 Size: 7558 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2419 3569 3916 6218 1239 Size: 902 278 1150 347 2302 2579 Fragments arranged by size: 2579 2302 1150 902 347 278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5683 6548 6846 5683 Size: 865 298 6395 Fragments arranged by size: 6395 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2879 3203 3273 4337 6221 882 Size: 617 1380 324 70 1064 1884 2219 Fragments arranged by size: 2219 1884 1380 1064 617 324 70 BsaAI yAC'GTr Cuts at: 493 1204 2374 2650 4288 4548 5349 493 Size: 711 1170 276 1638 260 801 2702 Fragments arranged by size: 2702 1638 1170 801 711 276 260 BsaBI GATnn'nnATC Cuts at: 7552 7552 Size: 7558 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 3459 3792 3819 Size: 53 83 186 936 603 1323 333 27 Cuts at: 3819 4635 5365 5747 6382 275 Size: 816 730 382 635 1451 Fragments arranged by size: 1451 1323 936 816 730 635 603 382 333 186 83 53 27 BsaWI w'CCGG_w Cuts at: 2888 3702 5991 6389 6663 6810 7221 2888 Size: 814 2289 398 274 147 411 3225 Fragments arranged by size: 3225 2289 814 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3092 3122 3283 3313 3332 Size: 30 165 30 1689 30 161 30 19 Cuts at: 3332 3362 3566 3596 3889 3919 4532 4562 1178 Size: 30 204 30 293 30 613 30 4174 Fragments arranged by size: 4174 1689 613 293 204 165 161 30 30 30 30 30 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2251 2347 2360 3118 3291 3518 3571 4553 Size: 1065 96 13 758 173 227 53 982 Cuts at: 4553 1186 Size: 4191 Fragments arranged by size: 4191 1065 982 758 227 173 96 53 13 BseYI C'CCAG_C Cuts at: 2031 2066 3021 3139 3187 3194 3391 3857 6761 Size: 35 955 118 48 7 197 466 2904 Cuts at: 6761 7257 2031 Size: 496 2332 Fragments arranged by size: 2904 2332 955 496 466 197 118 48 35 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2257 2330 2438 2626 3727 4183 4270 Size: 573 521 73 108 188 1101 456 87 Cuts at: 4270 1163 Size: 4451 Fragments arranged by size: 4451 1101 573 521 456 188 108 87 73 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3630 5008 5769 6797 1540 Size: 275 186 1629 1378 761 1028 2301 Fragments arranged by size: 2301 1629 1378 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3473 4097 4464 4547 4601 5619 5704 6775 3473 Size: 624 367 83 54 1018 85 1071 4256 Fragments arranged by size: 4256 1071 1018 624 367 85 83 54 BsiWI C'GTAC_G Cuts at: 3765 3765 Size: 7558 BsmI GAATG_Cn' Cuts at: 2680 5069 5162 2680 Size: 2389 93 5076 Fragments arranged by size: 5076 2389 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2362 3878 4237 4626 2362 Size: 1516 359 389 5294 Fragments arranged by size: 5294 1516 389 359 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 2258 2282 2565 2722 2919 3194 Size: 399 342 126 24 283 157 197 275 Cuts at: 3194 3258 3473 3729 4065 4097 4208 4464 4521 Size: 64 215 256 336 32 111 256 57 Cuts at: 4521 4547 4601 4737 4780 4809 4877 5619 5704 Size: 26 54 136 43 29 68 742 85 Cuts at: 5704 6775 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 342 336 283 275 256 256 215 197 157 136 126 111 85 68 64 57 54 43 32 29 26 24 BspEI T'CCGG_A Cuts at: 7221 7221 Size: 7558 BspHI T'CATG_A Cuts at: 5447 7177 5447 Size: 1730 5828 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2279 2460 2538 3537 3749 3923 4049 4439 Size: 1435 181 78 999 212 174 126 390 Cuts at: 4439 4616 4913 844 Size: 177 297 3489 Fragments arranged by size: 3489 1435 999 390 297 212 181 177 174 126 78 BsrBI CCG'CTC Cuts at: 1394 1823 2218 3136 4417 4836 5445 1394 Size: 429 395 918 1281 419 609 3507 Fragments arranged by size: 3507 1281 918 609 429 419 395 BsrDI GCAATG_nn' Cuts at: 65 1248 2027 6053 6227 65 Size: 1183 779 4026 174 1396 Fragments arranged by size: 4026 1396 1183 779 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2862 3153 3233 3702 4218 6202 6389 Size: 110 1052 291 80 469 516 1984 187 Cuts at: 6389 1700 Size: 2869 Fragments arranged by size: 2869 1984 1052 516 469 291 187 110 80 BsrGI T'GTAC_A Cuts at: 96 2376 3987 96 Size: 2280 1611 3667 Fragments arranged by size: 3667 2280 1611 BssHII G'CGCG_C Cuts at: 1925 3668 1925 Size: 1743 5815 BssSI C'ACGA_G Cuts at: 3008 3722 3845 5618 6630 3008 Size: 714 123 1773 1012 3936 Fragments arranged by size: 3936 1773 1012 714 123 BstAPI GCAn_nnn'nTGC Cuts at: 794 2227 2514 4041 4827 794 Size: 1433 287 1527 786 3525 Fragments arranged by size: 3525 1527 1433 786 287 BstBI TT'CG_AA Cuts at: 6362 6362 Size: 7558 BstEII G'GTnAC_C Cuts at: 1184 2698 4123 1184 Size: 1514 1425 4619 Fragments arranged by size: 4619 1514 1425 BstXI CCAn_nnnn'nTGG Cuts at: 2119 4354 4370 2119 Size: 2235 16 5307 Fragments arranged by size: 5307 2235 16 BstYI r'GATC_y Cuts at: 1972 4888 5255 5640 5657 7109 7195 7207 7553 Size: 2916 367 385 17 1452 86 12 346 Cuts at: 7553 1972 Size: 1977 Fragments arranged by size: 2916 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2091 2139 2466 2871 3690 4666 Size: 552 939 87 48 327 405 819 976 Cuts at: 4666 4728 513 Size: 62 3343 Fragments arranged by size: 3343 976 939 819 552 405 327 87 62 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2824 487 Size: 683 56 1598 5221 Fragments arranged by size: 5221 1598 683 56 BtsI GCAGTG_nn' Cuts at: 2221 2357 3739 4095 4123 4229 4258 4507 5074 Size: 136 1382 356 28 106 29 249 567 Cuts at: 5074 5868 5888 2221 Size: 794 20 3891 Fragments arranged by size: 3891 1382 794 567 356 249 136 106 29 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7558 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7523 DraI TTT'AAA Cuts at: 4862 4862 Size: 7558 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3287 6565 809 Size: 507 1971 3278 1802 Fragments arranged by size: 3278 1971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2473 3118 3163 Size: 54 1425 50 275 18 643 645 45 Cuts at: 3163 3477 3627 4356 4476 4782 5005 5894 7523 Size: 314 150 729 120 306 223 889 1629 Cuts at: 7523 8 Size: 43 Fragments arranged by size: 1629 1425 889 729 645 643 314 306 275 223 150 120 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3627 5005 1537 Size: 275 1815 1378 4090 Fragments arranged by size: 4090 1815 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3321 5488 1353 Size: 1968 2167 3423 Fragments arranged by size: 3423 2167 1968 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2310 2401 6145 6519 6665 387 Size: 1064 138 721 91 3744 374 146 1280 Fragments arranged by size: 3744 1280 1064 721 374 146 138 91 EcoICRI GAG'CTC Cuts at: 3471 3471 Size: 7558 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2419 3569 3916 5621 6218 7005 Size: 393 509 278 1150 347 1705 597 787 Cuts at: 7005 7273 1239 Size: 268 1524 Fragments arranged by size: 1705 1524 1150 787 597 509 393 347 278 268 EcoNI CCTnn'n_nnAGG Cuts at: 4001 4001 Size: 7558 EcoO109I rG'GnC_Cy Cuts at: 2255 2268 2915 2929 3064 3191 3243 3254 3255 Size: 13 647 14 135 127 52 11 1 Cuts at: 3255 3540 3589 4062 4560 4845 2255 Size: 285 49 473 498 285 4968 Fragments arranged by size: 4968 647 498 473 285 285 135 127 52 49 14 13 11 1 EcoRI G'AATT_C Cuts at: 4867 5245 4867 Size: 378 7180 FspI TGC'GCA Cuts at: 6064 6064 Size: 7558 HaeII r_GCGC'y Cuts at: 1678 3054 3462 6705 1678 Size: 1376 408 3243 2531 Fragments arranged by size: 3243 2531 1376 408 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2063 2095 2495 2527 4336 Size: 32 135 32 491 32 400 32 1809 Cuts at: 4336 4368 6199 6231 6273 6305 1373 Size: 32 1831 32 42 32 2626 Fragments arranged by size: 2626 1831 1809 491 400 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2082 3334 4902 5148 669 Size: 988 281 144 1252 1568 246 3079 Fragments arranged by size: 3079 1568 1252 988 281 246 144 HindIII A'AGCT_T Cuts at: 748 748 Size: 7558 HpaI GTT'AAC Cuts at: 5148 5148 Size: 7558 KasI G'GCGC_C Cuts at: 3458 3458 Size: 7558 KpnI G_GTAC'C Cuts at: 2296 4883 2296 Size: 2587 4971 MfeI C'AATT_G Cuts at: 5157 5157 Size: 7558 MluI A'CGCG_T Cuts at: 7434 7434 Size: 7558 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4059 4260 6672 6856 1305 Size: 2754 201 2412 184 2007 Fragments arranged by size: 2754 2412 2007 201 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4358 4478 4784 10 Size: 54 1425 343 2526 120 306 2784 Fragments arranged by size: 2784 2526 1425 343 306 120 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 4368 4790 5516 5875 6034 518 Size: 648 1482 1720 422 726 359 159 2042 Fragments arranged by size: 2042 1720 1482 726 648 422 359 159 MspA1I CmG'CkG Cuts at: 1606 2479 2640 2756 3388 3692 3894 4140 4749 Size: 873 161 116 632 304 202 246 609 Cuts at: 4749 4974 5651 6799 7044 1606 Size: 225 677 1148 245 2120 Fragments arranged by size: 2120 1148 873 677 632 609 304 246 245 225 202 161 116 NaeI GCC'GGC Cuts at: 3235 3235 Size: 7558 NarI GG'CG_CC Cuts at: 3459 3459 Size: 7558 NcoI C'CATG_G Cuts at: 513 1065 2004 2871 513 Size: 552 939 867 5200 Fragments arranged by size: 5200 939 867 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7558 NgoMIV G'CCGG_C Cuts at: 3233 3233 Size: 7558 NheI G'CTAG_C Cuts at: 1052 2764 1052 Size: 1712 5846 NotI GC'GGCC_GC Cuts at: 5005 5005 Size: 7558 NspI r_CATG'y Cuts at: 1132 1229 3700 4916 6461 1132 Size: 97 2471 1216 1545 2229 Fragments arranged by size: 2471 2229 1545 1216 97 PasI CC'CwG_GG Cuts at: 1348 3261 1348 Size: 1913 5645 PciI A'CATG_T Cuts at: 3696 6457 3696 Size: 2761 4797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2602 3067 3391 4399 4587 1108 Size: 1494 465 324 1008 188 4079 Fragments arranged by size: 4079 1494 1008 465 324 188 PmeI GTTT'AAAC Cuts at: 4862 4862 Size: 7558 PmlI CAC'GTG Cuts at: 2374 2650 4288 4548 2374 Size: 276 1638 260 5384 Fragments arranged by size: 5384 1638 276 260 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3889 3921 5627 5659 7140 7172 3889 Size: 32 1706 32 1481 32 4275 Fragments arranged by size: 4275 1706 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2268 3540 2268 Size: 1272 6286 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7558 PsiI TTA'TAA Cuts at: 5128 5128 Size: 7558 PspOMI G'GGCC_C Cuts at: 2254 2915 3190 3254 4061 4204 2254 Size: 661 275 64 807 143 5608 Fragments arranged by size: 5608 807 661 275 143 64 PstI C_TGCA'G Cuts at: 830 2232 2306 3000 3734 4719 4844 4910 830 Size: 1402 74 694 734 985 125 66 3478 Fragments arranged by size: 3478 1402 985 734 694 125 74 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7558 PvuII CAG'CTG Cuts at: 2640 3388 3894 4749 2640 Size: 748 506 855 5449 Fragments arranged by size: 5449 855 748 506 SacI G_AGCT'C Cuts at: 3473 3473 Size: 7558 SacII CC_GC'GG Cuts at: 3693 3693 Size: 7558 SalI G'TCGA_C Cuts at: 1936 4900 1936 Size: 2964 4594 SbfI CC_TGCA'GG Cuts at: 4844 4910 4844 Size: 66 7492 ScaI AGT'ACT Cuts at: 5806 7518 5806 Size: 1712 5846 SfcI C'TryA_G Cuts at: 826 1045 2228 2302 2996 3730 4182 4715 4816 Size: 219 1183 74 694 734 452 533 101 Cuts at: 4816 4840 4906 6041 6722 6913 826 Size: 24 66 1135 681 191 1471 Fragments arranged by size: 1471 1183 1135 734 694 681 533 452 219 191 101 74 66 24 SfiI GGCCn_nnn'nGGCC Cuts at: 3171 3171 Size: 7558 SfoI GGC'GCC Cuts at: 3460 3460 Size: 7558 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7558 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7558 SmaI CCC'GGG Cuts at: 1872 2253 2260 3171 4885 1872 Size: 381 7 911 1714 4545 Fragments arranged by size: 4545 1714 911 381 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 5662 6563 6825 820 Size: 197 926 3719 901 262 1553 Fragments arranged by size: 3719 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5349 493 Size: 4856 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7558 SphI G_CATG'C Cuts at: 1132 4916 1132 Size: 3784 3774 SspI AAT'ATT Cuts at: 5 52 5482 5 Size: 47 5430 2081 Fragments arranged by size: 5430 2081 47 StuI AGG'CCT Cuts at: 1517 3125 4180 4706 1517 Size: 1608 1055 526 4369 Fragments arranged by size: 4369 1608 1055 526 StyI C'CwwG_G Cuts at: 513 1065 2004 2683 2871 3464 3663 4997 513 Size: 552 939 679 188 593 199 1334 3074 Fragments arranged by size: 3074 1334 939 679 593 552 199 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2609 2660 3353 4670 5585 5602 5755 Size: 1255 1029 51 693 1317 915 17 153 Cuts at: 5755 5940 6385 325 Size: 185 445 1498 Fragments arranged by size: 1498 1317 1255 1029 915 693 445 185 153 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 2898 2985 Size: 274 80 33 51 1443 399 522 87 Cuts at: 2985 3987 4290 5804 7516 96 Size: 1002 303 1514 1712 138 Fragments arranged by size: 1712 1514 1443 1002 522 399 303 274 138 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3092 3124 3283 3315 3332 3364 4532 4564 7518 Size: 32 159 32 17 32 1168 32 2954 Cuts at: 7518 7550 3092 Size: 32 3100 Fragments arranged by size: 3100 2954 1168 159 32 32 32 32 32 17 Tth111I GACn'n_nGTC Cuts at: 1316 2423 4661 1316 Size: 1107 2238 4213 Fragments arranged by size: 4213 2238 1107 XbaI T'CTAG_A Cuts at: 4894 4894 Size: 7558 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2599 2711 4777 2599 Size: 112 2066 5380 Fragments arranged by size: 5380 2066 112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7558 XmaI C'CCGG_G Cuts at: 1870 2251 2258 3169 4883 1870 Size: 381 7 911 1714 4545 Fragments arranged by size: 4545 1714 911 381 7 XmnI GAAnn'nnTTC Cuts at: 3416 5687 3416 Size: 2271 5287 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4636 5366 276 Size: 53 83 186 936 3102 730 2468 Fragments arranged by size: 3102 2468 936 730 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI Bpu10I BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI