Restriction map for
pFN21AA0605
(Linear) (Six-base) MAPSORT of: KIAA0605.seq Check: 310 from: 1 to: 2853
KIAA0605.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1744 1918 2434 2853
Size: 1744 174 516 419
Fragments arranged by size:
1744 516 419 174
AatII G_ACGT'C
Cuts at: 0 2633 2853
Size: 2633 220
Acc65I G'GTAC_C
Cuts at: 0 287 2853
Size: 287 2566
AclI AA'CG_TT
Cuts at: 0 1598 2853
Size: 1598 1255
AfeI AGC'GCT
Cuts at: 0 1047 2853
Size: 1047 1806
AflIII A'CryG_T
Cuts at: 0 368 1691 2282 2542 2853
Size: 368 1323 591 260 311
Fragments arranged by size:
1323 591 368 311 260
AgeI A'CCGG_T
Cuts at: 0 1697 2853
Size: 1697 1156
AhdI GACnn_n'nnGTC
Cuts at: 0 72 2853
Size: 72 2781
AleI CACnn'nnGTG
Cuts at: 0 643 2363 2853
Size: 643 1720 490
Fragments arranged by size:
1720 643 490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1627 2853
Size: 1627 1226
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1884 1916 2853
Size: 1884 32 937
Fragments arranged by size:
1884 937 32
AlwNI CAG_nnn'CTG
Cuts at: 0 266 927 1062 1386 1399 1808 2582 2853
Size: 266 661 135 324 13 409 774 271
Fragments arranged by size:
774 661 409 324 271 266 135 13
ApaI G_GGCC'C
Cuts at: 0 253 914 1189 1253 2060 2203 2853
Size: 253 661 275 64 807 143 650
Fragments arranged by size:
807 661 650 275 253 143 64
ApaLI G'TGCA_C
Cuts at: 0 2088 2592 2853
Size: 2088 504 261
Fragments arranged by size:
2088 504 261
AvaI C'yCGr_G
Cuts at: 0 246 253 716 1164 1828 2020 2503 2530
Size: 246 7 463 448 664 192 483 27
Cuts at: 2530 2853
Size: 323
Fragments arranged by size:
664 483 463 448 323 246 192 27
7
BanI G'GyrC_C
Cuts at: 0 124 287 712 1453 2761 2853
Size: 124 163 425 741 1308 92
Fragments arranged by size:
1308 741 425 163 124 92
BanII G_rGCy'C
Cuts at: 0 253 560 914 1189 1253 1468 1724 2060
Size: 253 307 354 275 64 215 256 336
Cuts at: 2060 2203 2516 2732 2853
Size: 143 313 216 121
Fragments arranged by size:
354 336 313 307 275 256 253 216
215 143 121 64
BbeI G_GCGC'C
Cuts at: 0 1457 2853
Size: 1457 1396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 518 552 1546 1580 2074 2108 2529 2563
Size: 518 34 994 34 494 34 421 34
Cuts at: 2563 2853
Size: 290
Fragments arranged by size:
994 518 494 421 290 34 34 34
34
BglI GCCn_nnn'nGGC
Cuts at: 0 1166 2853
Size: 1166 1687
BlpI GC'TnA_GC
Cuts at: 0 1549 2853
Size: 1549 1304
Bme1580I G_kGCm'C
Cuts at: 0 127 253 277 717 914 1189 1253 2060
Size: 127 126 24 440 197 275 64 807
Cuts at: 2060 2092 2203 2596 2775 2804 2853
Size: 32 111 393 179 29 49
Fragments arranged by size:
807 440 393 275 197 179 127 126
111 64 49 32 29 24
BmgBI CAC'GTC
Cuts at: 0 278 756 2853
Size: 278 478 2097
Fragments arranged by size:
2097 478 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 2055 2244 2319 2415 2853
Size: 2055 189 75 96 438
Fragments arranged by size:
2055 438 189 96 75
BmtI G_CTAG'C
Cuts at: 0 763 2853
Size: 763 2090
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1374 2853
Size: 1374 1479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 414 1564 1911 2853
Size: 136 278 1150 347 942
Fragments arranged by size:
1150 942 347 278 136
BsaI GGTCTCn'nnnn_
Cuts at: 0 874 1198 1268 2332 2853
Size: 874 324 70 1064 521
Fragments arranged by size:
1064 874 521 324 70
BsaAI yAC'GTr
Cuts at: 0 369 645 2283 2543 2853
Size: 369 276 1638 260 310
Fragments arranged by size:
1638 369 310 276 260
BsaHI Gr'CG_yC
Cuts at: 0 131 1454 1787 1814 2630 2853
Size: 131 1323 333 27 816 223
Fragments arranged by size:
1323 816 333 223 131 27
BsaWI w'CCGG_w
Cuts at: 0 883 1697 2853
Size: 883 814 1156
Fragments arranged by size:
1156 883 814
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1087 1117 1278 1308 1327 1357 1561 1591
Size: 1087 30 161 30 19 30 204 30
Cuts at: 1591 1884 1914 2527 2557 2853
Size: 293 30 613 30 296
Fragments arranged by size:
1087 613 296 293 204 161 30 30
30 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 246 342 355 1113 1286 1513 1566 2548
Size: 246 96 13 758 173 227 53 982
Cuts at: 2548 2853
Size: 305
Fragments arranged by size:
982 758 305 246 227 173 96 53
13
BseYI C'CCAG_C
Cuts at: 0 26 61 1016 1134 1182 1189 1386 1852
Size: 26 35 955 118 48 7 197 466
Cuts at: 1852 2853
Size: 1001
Fragments arranged by size:
1001 955 466 197 118 48 35 26
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 325 433 621 1722 2178 2265 2853
Size: 252 73 108 188 1101 456 87 588
Fragments arranged by size:
1101 588 456 252 188 108 87 73
BsiEI CG_ry'CG
Cuts at: 0 1625 2853
Size: 1625 1228
BsiHKAI G_wGCw'C
Cuts at: 0 1468 2092 2459 2542 2596 2853
Size: 1468 624 367 83 54 257
Fragments arranged by size:
1468 624 367 257 83 54
BsiWI C'GTAC_G
Cuts at: 0 1760 2853
Size: 1760 1093
BsmI GAATG_Cn'
Cuts at: 0 675 2853
Size: 675 2178
BsmBI CGTCTCn'nnnn_
Cuts at: 0 357 1873 2232 2621 2853
Size: 357 1516 359 389 232
Fragments arranged by size:
1516 389 359 357 232
Bsp1286I G_dGCh'C
Cuts at: 0 127 253 277 560 717 914 1189 1253
Size: 127 126 24 283 157 197 275 64
Cuts at: 1253 1468 1724 2060 2092 2203 2459 2516 2542
Size: 215 256 336 32 111 256 57 26
Cuts at: 2542 2596 2732 2775 2804 2853
Size: 54 136 43 29 49
Fragments arranged by size:
336 283 275 256 256 215 197 157
136 127 126 111 64 57 54 49
43 32 29 26 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 274 455 533 1532 1744 1918 2044 2434
Size: 274 181 78 999 212 174 126 390
Cuts at: 2434 2611 2853
Size: 177 242
Fragments arranged by size:
999 390 274 242 212 181 177 174
126 78
BsrBI CCG'CTC
Cuts at: 0 213 1131 2412 2831 2853
Size: 213 918 1281 419 22
Fragments arranged by size:
1281 918 419 213 22
BsrDI GCAATG_nn'
Cuts at: 0 22 2853
Size: 22 2831
BsrFI r'CCGG_y
Cuts at: 0 857 1148 1228 1697 2213 2853
Size: 857 291 80 469 516 640
Fragments arranged by size:
857 640 516 469 291 80
BsrGI T'GTAC_A
Cuts at: 0 371 1982 2853
Size: 371 1611 871
Fragments arranged by size:
1611 871 371
BssHII G'CGCG_C
Cuts at: 0 1663 2853
Size: 1663 1190
BssSI C'ACGA_G
Cuts at: 0 1003 1717 1840 2853
Size: 1003 714 123 1013
Fragments arranged by size:
1013 1003 714 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 222 509 2036 2822 2853
Size: 222 287 1527 786 31
Fragments arranged by size:
1527 786 287 222 31
BstEII G'GTnAC_C
Cuts at: 0 693 2118 2853
Size: 693 1425 735
Fragments arranged by size:
1425 735 693
BstXI CCAn_nnnn'nTGG
Cuts at: 0 114 2349 2365 2853
Size: 114 2235 16 488
Fragments arranged by size:
2235 488 114 16
BtgI C'CryG_G
Cuts at: 0 86 134 461 866 1685 2661 2723 2853
Size: 86 48 327 405 819 976 62 130
Fragments arranged by size:
976 819 405 327 130 86 62 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 819 2853
Size: 819 2034
BtsI GCAGTG_nn'
Cuts at: 0 216 352 1734 2090 2118 2224 2253 2502
Size: 216 136 1382 356 28 106 29 249
Cuts at: 2502 2853
Size: 351
Fragments arranged by size:
1382 356 351 249 216 136 106 29
28
DrdI GACnn_nn'nnGTC
Cuts at: 0 1282 2853
Size: 1282 1571
EaeI y'GGCC_r
Cuts at: 0 468 1113 1158 1472 1622 2351 2471 2777
Size: 468 645 45 314 150 729 120 306
Cuts at: 2777 2853
Size: 76
Fragments arranged by size:
729 645 468 314 306 150 120 76
45
EagI C'GGCC_G
Cuts at: 0 1622 2853
Size: 1622 1231
EarI CTCTTCn'nnn_
Cuts at: 0 1316 2853
Size: 1316 1537
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 305 396 2853
Size: 305 91 2457
Fragments arranged by size:
2457 305 91
EcoICRI GAG'CTC
Cuts at: 0 1466 2853
Size: 1466 1387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 414 1564 1911 2853
Size: 136 278 1150 347 942
Fragments arranged by size:
1150 942 347 278 136
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1996 2853
Size: 1996 857
EcoO109I rG'GnC_Cy
Cuts at: 0 250 263 910 924 1059 1186 1238 1249
Size: 250 13 647 14 135 127 52 11
Cuts at: 1249 1250 1535 1584 2057 2555 2840 2853
Size: 1 285 49 473 498 285 13
Fragments arranged by size:
647 498 473 285 285 250 135 127
52 49 14 13 13 11 1
HaeII r_GCGC'y
Cuts at: 0 1049 1457 2853
Size: 1049 408 1396
Fragments arranged by size:
1396 1049 408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 490 522 2331 2363 2853
Size: 58 32 400 32 1809 32 490
Fragments arranged by size:
1809 490 400 58 32 32 32
HincII GTy'rAC
Cuts at: 0 77 1329 2853
Size: 77 1252 1524
Fragments arranged by size:
1524 1252 77
KasI G'GCGC_C
Cuts at: 0 1453 2853
Size: 1453 1400
KpnI G_GTAC'C
Cuts at: 0 291 2853
Size: 291 2562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2054 2255 2853
Size: 2054 201 598
Fragments arranged by size:
2054 598 201
MscI TGG'CCA
Cuts at: 0 2353 2473 2779 2853
Size: 2353 120 306 74
Fragments arranged by size:
2353 306 120 74
MslI CAynn'nnrTG
Cuts at: 0 643 2363 2785 2853
Size: 643 1720 422 68
Fragments arranged by size:
1720 643 422 68
MspA1I CmG'CkG
Cuts at: 0 474 635 751 1383 1687 1889 2135 2744
Size: 474 161 116 632 304 202 246 609
Cuts at: 2744 2853
Size: 109
Fragments arranged by size:
632 609 474 304 246 202 161 116
109
NaeI GCC'GGC
Cuts at: 0 1230 2853
Size: 1230 1623
NarI GG'CG_CC
Cuts at: 0 1454 2853
Size: 1454 1399
NcoI C'CATG_G
Cuts at: 0 866 2853
Size: 866 1987
NgoMIV G'CCGG_C
Cuts at: 0 1228 2853
Size: 1228 1625
NheI G'CTAG_C
Cuts at: 0 759 2853
Size: 759 2094
NspI r_CATG'y
Cuts at: 0 1695 2853
Size: 1695 1158
PasI CC'CwG_GG
Cuts at: 0 1256 2853
Size: 1256 1597
PciI A'CATG_T
Cuts at: 0 1691 2853
Size: 1691 1162
PflMI CCAn_nnn'nTGG
Cuts at: 0 597 1062 1386 2394 2582 2853
Size: 597 465 324 1008 188 271
Fragments arranged by size:
1008 597 465 324 271 188
PmlI CAC'GTG
Cuts at: 0 369 645 2283 2543 2853
Size: 369 276 1638 260 310
Fragments arranged by size:
1638 369 310 276 260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1884 1916 2853
Size: 1884 32 937
Fragments arranged by size:
1884 937 32
PpuMI rG'GwC_Cy
Cuts at: 0 263 1535 2853
Size: 263 1272 1318
Fragments arranged by size:
1318 1272 263
PspOMI G'GGCC_C
Cuts at: 0 249 910 1185 1249 2056 2199 2853
Size: 249 661 275 64 807 143 654
Fragments arranged by size:
807 661 654 275 249 143 64
PstI C_TGCA'G
Cuts at: 0 227 301 995 1729 2714 2839 2853
Size: 227 74 694 734 985 125 14
Fragments arranged by size:
985 734 694 227 125 74 14
PvuII CAG'CTG
Cuts at: 0 635 1383 1889 2744 2853
Size: 635 748 506 855 109
Fragments arranged by size:
855 748 635 506 109
SacI G_AGCT'C
Cuts at: 0 1468 2853
Size: 1468 1385
SacII CC_GC'GG
Cuts at: 0 1688 2853
Size: 1688 1165
SbfI CC_TGCA'GG
Cuts at: 0 2839 2853
Size: 2839 14
SfcI C'TryA_G
Cuts at: 0 223 297 991 1725 2177 2710 2811 2835
Size: 223 74 694 734 452 533 101 24
Cuts at: 2835 2853
Size: 18
Fragments arranged by size:
734 694 533 452 223 101 74 24
18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1166 2853
Size: 1166 1687
SfoI GGC'GCC
Cuts at: 0 1455 2853
Size: 1455 1398
SmaI CCC'GGG
Cuts at: 0 248 255 1166 2853
Size: 248 7 911 1687
Fragments arranged by size:
1687 911 248 7
StuI AGG'CCT
Cuts at: 0 1120 2175 2701 2853
Size: 1120 1055 526 152
Fragments arranged by size:
1120 1055 526 152
StyI C'CwwG_G
Cuts at: 0 678 866 1459 1658 2853
Size: 678 188 593 199 1195
Fragments arranged by size:
1195 678 593 199 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 604 655 1348 2665 2853
Size: 604 51 693 1317 188
Fragments arranged by size:
1317 693 604 188 51
TatI w'GTAC_w
Cuts at: 0 371 893 980 1982 2285 2853
Size: 371 522 87 1002 303 568
Fragments arranged by size:
1002 568 522 371 303 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1087 1119 1278 1310 1327 1359 2527 2559
Size: 1087 32 159 32 17 32 1168 32
Cuts at: 2559 2853
Size: 294
Fragments arranged by size:
1168 1087 294 159 32 32 32 32
17
Tth111I GACn'n_nGTC
Cuts at: 0 418 2656 2853
Size: 418 2238 197
Fragments arranged by size:
2238 418 197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 594 706 2772 2853
Size: 594 112 2066 81
Fragments arranged by size:
2066 594 112 81
XmaI C'CCGG_G
Cuts at: 0 246 253 1164 2853
Size: 246 7 911 1689
Fragments arranged by size:
1689 911 246 7
XmnI GAAnn'nnTTC
Cuts at: 0 1411 2853
Size: 1411 1442
ZraI GAC'GTC
Cuts at: 0 2631 2853
Size: 2631 222
Enzymes that do cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BcgI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BtgI BtgZI BtsI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SacII SbfI SfcI SfiI SfoI SmaI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AcuI AflII ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
Bpu10I BpuEI BsaBI BspEI BspHI BstBI BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI MfeI MluI NdeI
NotI NruI NsiI PacI PfoI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI
SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI