Restriction map for
pFN21AA0586
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspI
AviII AvrII BciVI BcnI BfrI
BglI BlnI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspEI BsrBI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI Bsu36I CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HgaI KpnI KspI MfeI
MluI MroI MunI MvnI NciI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 318 GTATAC GT^[AC][GT]AC
AcyI 2917 GGCGCC G[GA]^CG[CT]C
AhdI 4570 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3108 GTGCAC G^TGCAC
ApaLI 3108 GTGCAC G^TGCAC
AspEI 4570 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4525 CTCGAG C^[CT]CG[GA]G
BalI 1281 TGGCCA TGG^CCA
BamHI 2373 GGATCC G^GATCC
BanII 2815 GGGCTC G[GA]GC[CT]^C
BbeI 2917 GGCGCC GGCGC^C
BbrPI 149 CACGTG CAC^GTG
BbvCI 2380 CCTCAGC CCTCAGC(-5/-2)
BcgI 2985 CGAAGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 3532 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3532 GCTCAGC GC^T[ACGT]AGC
BsaHI 2917 GGCGCC G[GA]^CG[CT]C
BsmI 644 GCATTC GAATGC(1/-1)
BsoBI 4525 CTCGAG C^[CT]CG[GA]G
BspDI 2575 ATCGAT AT^CGAT
BspLU11I 4435 ACATGT A^CATGT
Bst1107I 318 GTATAC GTA^TAC
BstXI 824 CCAGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 318 GTATAC GTA^TAC
CelII 3532 GCTCAGC GC^T[ACGT]AGC
CfoI 2918 GCGC GCG^C
ClaI 2575 ATCGAT AT^CGAT
EaeI 1281 TGGCCA [CT]^GGCC[GA]
Eam1105I 4570 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3826 CCTAGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1362 GAATTC G^AATTC
HaeII 2917 GGCGCC [GA]GCGC^[CT]
HhaI 2918 GCGC GCG^C
Hin1I 2917 GGCGCC G[GA]^CG[CT]C
HinP1I 2918 GCGC G^CGC
HpaI 2980 GTTAAC GTT^AAC
KasI 2917 GGCGCC G^GCGCC
MluNI 1281 TGGCCA TGG^CCA
MscI 1281 TGGCCA TGG^CCA
NarI 2917 GGCGCC GG^CGCC
PaeR7I 4525 CTCGAG C^TCGAG
PmaCI 149 CACGTG CAC^GTG
PmlI 149 CACGTG CAC^GTG
PpuMI 3042 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 1452 AGTACT AGT^ACT
SexAI 4389 ACCAGGT A^CC[AT]GGT
SfoI 2917 GGCGCC GGC^GCC
SspI 2126 AATATT AAT^ATT
XhoI 4525 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspHI 3108 GTGCAC G[AT]GC[AT]^C
3636 GAGCAC
BanI 2917 GGCGCC G^G[CT][GA]CC
3580 GGCACC
BbsI 110 GTCTTC GAAGAC(2/6)
4578 GTCTTC
BclI 678 TGATCA T^GATCA
1650 TGATCA
BglII 1350 AGATCT A^GATCT
1396 AGATCT
BpuAI 110 GTCTTC GAAGAC(2/6)
4578 GTCTTC
BsaAI 149 CACGTG [CT]AC^GT[GA]
4340 TACGTG
BsaBI 3208 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4396 GATATGGATC
BsiHKAI 3108 GTGCAC G[AT]GC[AT]^C
3636 GAGCAC
BsrDI 513 CATTGC GCAATG(2/0)
3003 CATTGC
BsrFI 3096 GCCGGC [GA]^CCGG[CT]
3577 GCCGGC
Cfr10I 3096 GCCGGC [GA]^CCGG[CT]
3577 GCCGGC
DraI 354 TTTAAA TTT^AAA
921 TTTAAA
DraII 3042 AGGTCCT [GA]G^G[ACGT]CC[CT]
3587 AGGCCCC
DraIII 461 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4388 CACCAGGTG
EcoO109I 3042 AGGTCCT [GA]G^G[ACGT]CC[CT]
3587 AGGCCCC
FbaI 678 TGATCA T^GATCA
1650 TGATCA
HincII 1726 GTCAAC GT[CT]^[GA]AC
2980 GTTAAC
HindII 1726 GTCAAC GT[CT]^[GA]AC
2980 GTTAAC
HindIII 1342 AAGCTT A^AGCTT
1372 AAGCTT
MamI 3208 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4396 GATATGGATC
NaeI 3096 GCCGGC GCC^GGC
3577 GCCGGC
NcoI 209 CCATGG C^CATGG
3406 CCATGG
NgoMIV 3096 GCCGGC G^CCGGC
3577 GCCGGC
PflMI 2239 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3401 CCAAGCCATGG
PstI 494 CTGCAG CTGCA^G
1598 CTGCAG
SapI 1222 GAAGAGC GCTCTTC(1/4)
1771 GAAGAGC
SpeI 130 ACTAGT A^CTAGT
790 ACTAGT
SphI 374 GCATGC GCATG^C
1802 GCATGC
Van91I 2239 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3401 CCAAGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BbvI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI