Restriction map for
pFN21AA0586
(Linear) (Six-base) MAPSORT of: KIAA0586.seq Check: 4208 from: 1 to: 4599
KIAA0586.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 623 2250 3168 4599
Size: 623 1627 918 1431
Fragments arranged by size:
1627 1431 918 623
AccI GT'mk_AC
Cuts at: 0 319 4599
Size: 319 4280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3710 4283 4599
Size: 3710 573 316
Fragments arranged by size:
3710 573 316
AflIII A'CryG_T
Cuts at: 0 621 4341 4435 4599
Size: 621 3720 94 164
Fragments arranged by size:
3720 621 164 94
AhdI GACnn_n'nnGTC
Cuts at: 0 4575 4599
Size: 4575 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2855 3123 4599
Size: 2855 268 1476
Fragments arranged by size:
2855 1476 268
AlwNI CAG_nnn'CTG
Cuts at: 0 502 2620 3616 4545 4599
Size: 502 2118 996 929 54
Fragments arranged by size:
2118 996 929 502 54
ApaLI G'TGCA_C
Cuts at: 0 3108 4599
Size: 3108 1491
ApoI r'AATT_y
Cuts at: 0 455 1106 1362 1604 2609 3862 4191 4599
Size: 455 651 256 242 1005 1253 329 408
Fragments arranged by size:
1253 1005 651 455 408 329 256 242
AvaI C'yCGr_G
Cuts at: 0 4525 4599
Size: 4525 74
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 391 424 4599
Size: 391 33 4175
Fragments arranged by size:
4175 391 33
BamHI G'GATC_C
Cuts at: 0 2373 4599
Size: 2373 2226
BanI G'GyrC_C
Cuts at: 0 2917 3580 4599
Size: 2917 663 1019
Fragments arranged by size:
2917 1019 663
BanII G_rGCy'C
Cuts at: 0 2819 4599
Size: 2819 1780
BbeI G_GCGC'C
Cuts at: 0 2921 4599
Size: 2921 1678
BbsI GAAGACnn'nnnn_
Cuts at: 0 103 4571 4599
Size: 103 4468 28
Fragments arranged by size:
4468 103 28
BbvCI CC'TCA_GC
Cuts at: 0 2381 4599
Size: 2381 2218
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2974 3008 4599
Size: 2974 34 1591
Fragments arranged by size:
2974 1591 34
BclI T'GATC_A
Cuts at: 0 678 1650 4599
Size: 678 972 2949
Fragments arranged by size:
2949 972 678
BglII A'GATC_T
Cuts at: 0 1350 1396 4599
Size: 1350 46 3203
Fragments arranged by size:
3203 1350 46
BlpI GC'TnA_GC
Cuts at: 0 3533 4599
Size: 3533 1066
Bme1580I G_kGCm'C
Cuts at: 0 3112 4599
Size: 3112 1487
BmrI ACTGGGnnnn_n'
Cuts at: 0 458 2399 4269 4599
Size: 458 1941 1870 330
Fragments arranged by size:
1941 1870 458 330
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4551 4599
Size: 4551 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 2267 2342 2495 2653 2908 3173 3308
Size: 807 1460 75 153 158 255 265 135
Cuts at: 3308 3530 3652 4599
Size: 222 122 947
Fragments arranged by size:
1460 947 807 265 255 222 158 153
135 122 75
Bpu10I CC'TnA_GC
Cuts at: 0 2381 3164 3497 4599
Size: 2381 783 333 1102
Fragments arranged by size:
2381 1102 783 333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 3440 4599
Size: 769 2671 1159
Fragments arranged by size:
2671 1159 769
BsaI GGTCTCn'nnnn_
Cuts at: 0 1980 2992 3413 4599
Size: 1980 1012 421 1186
Fragments arranged by size:
1980 1186 1012 421
BsaAI yAC'GTr
Cuts at: 0 151 4342 4599
Size: 151 4191 257
Fragments arranged by size:
4191 257 151
BsaBI GATnn'nnATC
Cuts at: 0 3212 4400 4599
Size: 3212 1188 199
Fragments arranged by size:
3212 1188 199
BsaHI Gr'CG_yC
Cuts at: 0 2918 4599
Size: 2918 1681
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 510 540 2240 2270 3303 3306 3333 3336
Size: 510 30 1700 30 1033 3 27 3
Cuts at: 3336 4599
Size: 1263
Fragments arranged by size:
1700 1263 1033 510 30 30 27 3
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 810 1445 2369 2471 2474 3311 4599
Size: 810 635 924 102 3 837 1288
Fragments arranged by size:
1288 924 837 810 635 102 3
BseYI C'CCAG_C
Cuts at: 0 3015 3920 3989 4599
Size: 3015 905 69 610
Fragments arranged by size:
3015 905 610 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 764 2413 4599
Size: 562 202 1649 2186
Fragments arranged by size:
2186 1649 562 202
BsiHKAI G_wGCw'C
Cuts at: 0 3112 3640 4599
Size: 3112 528 959
Fragments arranged by size:
3112 959 528
BsmI GAATG_Cn'
Cuts at: 0 644 4599
Size: 644 3955
Bsp1286I G_dGCh'C
Cuts at: 0 2819 3112 3640 4599
Size: 2819 293 528 959
Fragments arranged by size:
2819 959 528 293
BspHI T'CATG_A
Cuts at: 0 675 1272 3216 4599
Size: 675 597 1944 1383
Fragments arranged by size:
1944 1383 675 597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 623 2250 2385 3168 3431 4599
Size: 623 1627 135 783 263 1168
Fragments arranged by size:
1627 1168 783 623 263 135
BsrDI GCAATG_nn'
Cuts at: 0 512 3002 4599
Size: 512 2490 1597
Fragments arranged by size:
2490 1597 512
BsrFI r'CCGG_y
Cuts at: 0 3096 3577 4599
Size: 3096 481 1022
Fragments arranged by size:
3096 1022 481
BstAPI GCAn_nnn'nTGC
Cuts at: 0 97 255 502 505 4599
Size: 97 158 247 3 4094
Fragments arranged by size:
4094 247 158 97 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 4599
Size: 831 3768
BstYI r'GATC_y
Cuts at: 0 1350 1396 2373 4599
Size: 1350 46 977 2226
Fragments arranged by size:
2226 1350 977 46
BstZ17I GTA'TAC
Cuts at: 0 320 4599
Size: 320 4279
BtgI C'CryG_G
Cuts at: 0 209 488 3406 4599
Size: 209 279 2918 1193
Fragments arranged by size:
2918 1193 279 209
BtsI GCAGTG_nn'
Cuts at: 0 251 836 2714 2919 4466 4599
Size: 251 585 1878 205 1547 133
Fragments arranged by size:
1878 1547 585 251 205 133
ClaI AT'CG_AT
Cuts at: 0 2576 4599
Size: 2576 2023
DraI TTT'AAA
Cuts at: 0 356 923 4599
Size: 356 567 3676
Fragments arranged by size:
3676 567 356
DraIII CAC_nnn'GTG
Cuts at: 0 466 4393 4599
Size: 466 3927 206
Fragments arranged by size:
3927 466 206
EaeI y'GGCC_r
Cuts at: 0 1281 4599
Size: 1281 3318
EarI CTCTTCn'nnn_
Cuts at: 0 4 269 1217 1766 2531 3431 4599
Size: 4 265 948 549 765 900 1168
Fragments arranged by size:
1168 948 900 765 549 265 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2678 4599
Size: 2678 1921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 2267 2342 2495 2653 2908 3173 3308
Size: 807 1460 75 153 158 255 265 135
Cuts at: 3308 3530 3652 3710 4283 4599
Size: 222 122 58 573 316
Fragments arranged by size:
1460 807 573 316 265 255 222 158
153 135 122 75 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3830 4599
Size: 3830 769
EcoO109I rG'GnC_Cy
Cuts at: 0 3043 3588 4599
Size: 3043 545 1011
Fragments arranged by size:
3043 1011 545
EcoRI G'AATT_C
Cuts at: 0 1362 4599
Size: 1362 3237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3481 4599
Size: 3481 1118
HaeII r_GCGC'y
Cuts at: 0 2921 4599
Size: 2921 1678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1009 1023 1041 1055 1603 1635 1931 1963
Size: 1009 14 18 14 548 32 296 32
Cuts at: 1963 2240 2272 2493 2525 3766 3798 4016 4048
Size: 277 32 221 32 1241 32 218 32
Cuts at: 4048 4561 4593 4599
Size: 513 32 6
Fragments arranged by size:
1241 1009 548 513 296 277 221 218
32 32 32 32 32 32 32 18
14 14 6
HincII GTy'rAC
Cuts at: 0 1728 2982 4599
Size: 1728 1254 1617
Fragments arranged by size:
1728 1617 1254
HindIII A'AGCT_T
Cuts at: 0 1342 1372 4599
Size: 1342 30 3227
Fragments arranged by size:
3227 1342 30
HpaI GTT'AAC
Cuts at: 0 2982 4599
Size: 2982 1617
KasI G'GCGC_C
Cuts at: 0 2917 4599
Size: 2917 1682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 3319 4163 4599
Size: 99 3220 844 436
Fragments arranged by size:
3220 844 436 99
MscI TGG'CCA
Cuts at: 0 1283 4599
Size: 1283 3316
MslI CAynn'nnrTG
Cuts at: 0 674 3872 4018 4599
Size: 674 3198 146 581
Fragments arranged by size:
3198 674 581 146
MspA1I CmG'CkG
Cuts at: 0 3328 4000 4261 4599
Size: 3328 672 261 338
Fragments arranged by size:
3328 672 338 261
NaeI GCC'GGC
Cuts at: 0 3098 3579 4599
Size: 3098 481 1020
Fragments arranged by size:
3098 1020 481
NarI GG'CG_CC
Cuts at: 0 2918 4599
Size: 2918 1681
NcoI C'CATG_G
Cuts at: 0 209 3406 4599
Size: 209 3197 1193
Fragments arranged by size:
3197 1193 209
NgoMIV G'CCGG_C
Cuts at: 0 3096 3577 4599
Size: 3096 481 1022
Fragments arranged by size:
3096 1022 481
NspI r_CATG'y
Cuts at: 0 378 1806 4439 4599
Size: 378 1428 2633 160
Fragments arranged by size:
2633 1428 378 160
PciI A'CATG_T
Cuts at: 0 4435 4599
Size: 4435 164
PflMI CCAn_nnn'nTGG
Cuts at: 0 2245 3407 4599
Size: 2245 1162 1192
Fragments arranged by size:
2245 1192 1162
PfoI T'CCnGG_A
Cuts at: 0 2550 4599
Size: 2550 2049
PmlI CAC'GTG
Cuts at: 0 151 4599
Size: 151 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3433 3465 4599
Size: 3433 32 1134
Fragments arranged by size:
3433 1134 32
PpuMI rG'GwC_Cy
Cuts at: 0 3043 4599
Size: 3043 1556
PspXI vC'TCGA_Gb
Cuts at: 0 4525 4599
Size: 4525 74
PstI C_TGCA'G
Cuts at: 0 498 1602 4599
Size: 498 1104 2997
Fragments arranged by size:
2997 1104 498
SapI GCTCTTCn'nnn_
Cuts at: 0 1217 1766 4599
Size: 1217 549 2833
Fragments arranged by size:
2833 1217 549
ScaI AGT'ACT
Cuts at: 0 1454 4599
Size: 1454 3145
SexAI A'CCwGG_T
Cuts at: 0 4389 4599
Size: 4389 210
SfcI C'TryA_G
Cuts at: 0 494 1598 3053 4599
Size: 494 1104 1455 1546
Fragments arranged by size:
1546 1455 1104 494
SfoI GGC'GCC
Cuts at: 0 2919 4599
Size: 2919 1680
SmlI C'TyrA_G
Cuts at: 0 784 3455 4525 4599
Size: 784 2671 1070 74
Fragments arranged by size:
2671 1070 784 74
SpeI A'CTAG_T
Cuts at: 0 130 790 4599
Size: 130 660 3809
Fragments arranged by size:
3809 660 130
SphI G_CATG'C
Cuts at: 0 378 1806 4599
Size: 378 1428 2793
Fragments arranged by size:
2793 1428 378
SspI AAT'ATT
Cuts at: 0 2128 4599
Size: 2128 2471
StyI C'CwwG_G
Cuts at: 0 209 1278 3406 3584 4599
Size: 209 1069 2128 178 1015
Fragments arranged by size:
2128 1069 1015 209 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 3007 3952 4599
Size: 477 2530 945 647
Fragments arranged by size:
2530 945 647 477
TatI w'GTAC_w
Cuts at: 0 1434 1452 4599
Size: 1434 18 3147
Fragments arranged by size:
3147 1434 18
XhoI C'TCGA_G
Cuts at: 0 4525 4599
Size: 4525 74
XmnI GAAnn'nnTTC
Cuts at: 0 474 1752 2627 3464 4599
Size: 474 1278 875 837 1135
Fragments arranged by size:
1278 1135 875 837 474
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstXI BstYI BstZ17I BtgI BtsI
ClaI DraI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmlI PpiI PpuMI
PspXI PstI SapI ScaI SexAI SfcI SfoI SmlI
SpeI SphI SspI StyI TaqII TatI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
ApaI AscI AseI AsiSI AvrII BciVI BfrBI BglI
BmgBI BmtI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstBI BstEII Bsu36I BtgZI CspCI
DrdI EagI EcoICRI EcoRV FseI FspI FspAI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PasI PmeI PshAI PsiI PspOMI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI SfiI SgfI
SgrAI SmaI SnaBI SrfI StuI SwaI TstI Tth111I
XbaI XcmI XmaI ZraI