(Circular) (Six-base) MAPSORT of: pFN21AA0569.seq Check: 657 from: 1 to: 8347 pFN21AA0569 8347 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2913 2913 Size: 8347 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6157 278 Size: 53 83 186 936 4621 2468 Fragments arranged by size: 4621 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4095 5690 1937 Size: 2158 1595 4594 Fragments arranged by size: 4594 2158 1595 Acc65I G'GTAC_C Cuts at: 5668 5668 Size: 8347 AclI AA'CG_TT Cuts at: 1563 2956 3805 6474 6847 1563 Size: 1393 849 2669 373 3063 Fragments arranged by size: 3063 2669 1393 849 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2489 2702 3465 6410 7794 8062 1632 Size: 857 213 763 2945 1384 268 1917 Fragments arranged by size: 2945 1917 1384 857 763 268 213 AfeI AGC'GCT Cuts at: 2669 5192 2669 Size: 2523 5824 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8150 AflIII A'CryG_T Cuts at: 1203 2221 2748 3969 4920 7246 8223 1203 Size: 1018 527 1221 951 2326 977 1327 Fragments arranged by size: 2326 1327 1221 1018 977 951 527 AgeI A'CCGG_T Cuts at: 7178 7178 Size: 8347 AhdI GACnn_n'nnGTC Cuts at: 3970 4921 7076 3970 Size: 951 2155 5241 Fragments arranged by size: 5241 2155 951 AleI CACnn'nnGTG Cuts at: 1166 2919 3321 4251 5086 1166 Size: 1753 402 930 835 4427 Fragments arranged by size: 4427 1753 930 835 402 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8347 AlwNI CAG_nnn'CTG Cuts at: 2111 6090 7662 2111 Size: 3979 1572 2796 Fragments arranged by size: 3979 2796 1572 ApaLI G'TGCA_C Cuts at: 6404 7560 6404 Size: 1156 7191 ApoI r'AATT_y Cuts at: 1493 2910 3450 4032 4061 4108 4607 4615 5656 Size: 1417 540 582 29 47 499 8 1041 Cuts at: 5656 5882 6034 8143 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1417 1041 582 540 499 226 152 47 29 8 AseI AT'TA_AT Cuts at: 160 1032 6901 160 Size: 872 5869 1606 Fragments arranged by size: 5869 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8347 AvaI C'yCGr_G Cuts at: 1870 1943 2771 5152 5203 5672 1870 Size: 73 828 2381 51 469 4545 Fragments arranged by size: 4545 2381 828 469 73 51 BamHI G'GATC_C Cuts at: 3481 5527 5677 6044 3481 Size: 2046 150 367 5784 Fragments arranged by size: 5784 2046 367 150 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3563 5668 7123 618 Size: 325 217 627 1776 2105 1455 1842 Fragments arranged by size: 2105 1842 1776 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4011 4437 5210 5666 1391 Size: 2620 426 773 456 4072 Fragments arranged by size: 4072 2620 773 456 426 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2133 2355 3533 4988 5631 928 Size: 971 234 222 1178 1455 643 3644 Fragments arranged by size: 3644 1455 1178 971 643 234 222 BbvCI CC'TCA_GC Cuts at: 2145 2145 Size: 8347 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2462 2496 2752 2786 6538 6572 1903 Size: 34 525 34 256 34 3752 34 3678 Fragments arranged by size: 3752 3678 525 256 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5515 6239 7449 5515 Size: 724 1210 6413 Fragments arranged by size: 6413 1210 724 BclI T'GATC_A Cuts at: 1548 1548 Size: 8347 BfrBI ATG'CAT Cuts at: 5003 5003 Size: 8347 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6958 136 Size: 107 122 71 631 605 5286 1525 Fragments arranged by size: 5286 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8342 8342 Size: 8347 BlpI GC'TnA_GC Cuts at: 4443 5764 4443 Size: 1321 7026 Bme1580I G_kGCm'C Cuts at: 1790 2202 2942 5304 6408 7564 1790 Size: 412 740 2362 1104 1156 2573 Fragments arranged by size: 2573 2362 1156 1104 740 412 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8347 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3100 4643 6078 Size: 416 53 83 399 24 1683 1543 1435 Cuts at: 6078 7036 442 Size: 958 1753 Fragments arranged by size: 1753 1683 1543 1435 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8347 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2250 4030 4365 2250 Size: 1780 335 6232 Fragments arranged by size: 6232 1780 335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2483 2598 3608 4321 4421 4848 5580 7007 Size: 1244 115 1010 713 100 427 732 1427 Cuts at: 7007 1239 Size: 2579 Fragments arranged by size: 2579 1427 1244 1010 732 713 427 115 100 Bpu10I CC'TnA_GC Cuts at: 2145 2145 Size: 8347 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3628 5125 6472 7337 7635 3628 Size: 1497 1347 865 298 4340 Fragments arranged by size: 4340 1497 1347 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4200 7010 882 Size: 617 2701 2810 2219 Fragments arranged by size: 2810 2701 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2224 3683 6138 493 Size: 711 1020 1459 2455 2702 Fragments arranged by size: 2702 2455 1459 1020 711 BsaBI GATnn'nnATC Cuts at: 5526 8341 5526 Size: 2815 5532 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6154 6536 7171 275 Size: 53 83 186 936 4621 382 635 1451 Fragments arranged by size: 4621 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3544 6780 7178 7452 7599 8010 3544 Size: 3236 398 274 147 411 3881 Fragments arranged by size: 3881 3236 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3873 3903 4339 4369 4937 Size: 30 165 30 2470 30 436 30 568 Cuts at: 4937 4967 1178 Size: 30 4558 Fragments arranged by size: 4558 2470 568 436 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2510 2561 3896 4263 4445 5276 5393 5492 Size: 1324 51 1335 367 182 831 117 99 Cuts at: 5492 5501 1186 Size: 9 4032 Fragments arranged by size: 4032 1335 1324 831 367 182 117 99 51 9 BseYI C'CCAG_C Cuts at: 2766 5455 7550 8046 2766 Size: 2689 2095 496 3067 Fragments arranged by size: 3067 2689 2095 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7774 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2656 5797 6558 7586 1540 Size: 275 186 655 3141 761 1028 2301 Fragments arranged by size: 3141 2301 1028 761 655 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 4437 4912 5139 5424 6408 6493 7564 2691 Size: 1746 475 227 285 984 85 1071 3474 Fragments arranged by size: 3474 1746 1071 984 475 285 227 85 BsmI GAATG_Cn' Cuts at: 2419 5858 5951 2419 Size: 3439 93 4815 Fragments arranged by size: 4815 3439 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2174 2174 Size: 8347 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2691 2942 4011 4437 4912 5139 Size: 399 412 489 251 1069 426 475 227 Cuts at: 5139 5210 5304 5424 5666 6408 6493 7564 1391 Size: 71 94 120 242 742 85 1071 2174 Fragments arranged by size: 2174 1071 1069 742 489 475 426 412 399 251 242 227 120 94 85 71 BspEI T'CCGG_A Cuts at: 8010 8010 Size: 8347 BspHI T'CATG_A Cuts at: 3853 6236 7966 3853 Size: 2383 1730 4234 Fragments arranged by size: 4234 2383 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2607 2648 2825 2913 5702 844 Size: 1763 41 177 88 2789 3489 Fragments arranged by size: 3489 2789 1763 177 88 41 BsrBI CCG'CTC Cuts at: 1394 1823 2776 5266 6234 1394 Size: 429 953 2490 968 3507 Fragments arranged by size: 3507 2490 968 953 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4038 6842 7016 65 Size: 1183 2790 2804 174 1396 Fragments arranged by size: 2804 2790 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6991 7178 1700 Size: 110 5181 187 2869 Fragments arranged by size: 5181 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8347 BssHII G'CGCG_C Cuts at: 1925 5288 1925 Size: 3363 4984 BssSI C'ACGA_G Cuts at: 2705 5423 5618 6407 7419 2705 Size: 2718 195 789 1012 3633 Fragments arranged by size: 3633 2718 1012 789 195 BstAPI GCAn_nnn'nTGC Cuts at: 794 2818 3992 794 Size: 2024 1174 5149 Fragments arranged by size: 5149 2024 1174 BstBI TT'CG_AA Cuts at: 7151 7151 Size: 8347 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8347 BstXI CCAn_nnnn'nTGG Cuts at: 2170 4045 2170 Size: 1875 6472 BstYI r'GATC_y Cuts at: 1972 3481 5527 5677 6044 6429 6446 7898 7984 Size: 1509 2046 150 367 385 17 1452 86 Cuts at: 7984 7996 8342 1972 Size: 12 346 1977 Fragments arranged by size: 2046 1977 1509 1452 385 367 346 150 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2544 3498 4056 5358 2544 Size: 954 558 1302 5533 Fragments arranged by size: 5533 1302 954 558 BtgI C'CryG_G Cuts at: 513 1065 5571 513 Size: 552 4506 3289 Fragments arranged by size: 4506 3289 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2487 487 Size: 683 56 1261 6347 Fragments arranged by size: 6347 1261 683 56 BtsI GCAGTG_nn' Cuts at: 2518 5863 6657 6677 2518 Size: 3345 794 20 4188 Fragments arranged by size: 4188 3345 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8312 DraI TTT'AAA Cuts at: 5651 5651 Size: 8347 DraIII CAC_nnn'GTG Cuts at: 2862 2862 Size: 8347 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7354 809 Size: 507 6038 1802 Fragments arranged by size: 6038 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3028 5794 6683 Size: 54 1425 50 275 18 1198 2766 889 Cuts at: 6683 8312 8 Size: 1629 43 Fragments arranged by size: 2766 1629 1425 1198 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5794 1537 Size: 275 3982 4090 Fragments arranged by size: 4090 3982 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2247 2712 3822 4429 4701 5487 5547 6277 Size: 894 465 1110 607 272 786 60 730 Cuts at: 6277 1353 Size: 3423 Fragments arranged by size: 3423 1110 894 786 730 607 465 272 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 3519 5273 5291 6934 7308 Size: 1064 138 450 1480 1754 18 1643 374 Cuts at: 7308 7454 387 Size: 146 1280 Fragments arranged by size: 1754 1643 1480 1280 1064 450 374 146 138 18 EcoICRI GAG'CTC Cuts at: 4435 4435 Size: 8347 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2483 2489 2598 2702 3465 3608 4321 Size: 393 851 6 109 104 763 143 713 Cuts at: 4321 4421 4848 5580 6410 7007 7794 8062 1239 Size: 100 427 732 830 597 787 268 1524 Fragments arranged by size: 1524 851 830 787 763 732 713 597 427 393 268 143 109 104 100 6 EcoNI CCTnn'n_nnAGG Cuts at: 4055 4055 Size: 8347 EcoO109I rG'GnC_Cy Cuts at: 4116 4116 Size: 8347 EcoRI G'AATT_C Cuts at: 2910 5656 6034 2910 Size: 2746 378 5223 Fragments arranged by size: 5223 2746 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2150 2150 Size: 8347 FspI TGC'GCA Cuts at: 6853 6853 Size: 8347 HaeII r_GCGC'y Cuts at: 1678 2671 5194 7494 1678 Size: 993 2523 2300 2531 Fragments arranged by size: 2531 2523 2300 993 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 3473 3505 3956 Size: 32 135 32 965 32 904 32 451 Cuts at: 3956 3988 4907 4937 4939 4969 5548 5580 6988 Size: 32 919 30 2 30 579 32 1408 Cuts at: 6988 7020 7062 7094 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1408 965 919 904 579 451 135 42 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 5691 5937 669 Size: 988 281 3753 246 3079 Fragments arranged by size: 3753 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2132 3615 748 Size: 1384 1483 5480 Fragments arranged by size: 5480 1483 1384 HpaI GTT'AAC Cuts at: 5937 5937 Size: 8347 KpnI G_GTAC'C Cuts at: 5672 5672 Size: 8347 MfeI C'AATT_G Cuts at: 5946 5946 Size: 8347 MluI A'CGCG_T Cuts at: 8223 8223 Size: 8347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3102 3573 4974 7461 7645 1305 Size: 1797 471 1401 2487 184 2007 Fragments arranged by size: 2487 2007 1797 1401 471 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6525 Fragments arranged by size: 6525 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2919 3321 3561 3748 3798 4043 4251 Size: 648 1753 402 240 187 50 245 208 Cuts at: 4251 4545 5086 6305 6664 6823 518 Size: 294 541 1219 359 159 2042 Fragments arranged by size: 2042 1753 1219 648 541 402 359 294 245 240 208 187 159 50 MspA1I CmG'CkG Cuts at: 1606 4873 5763 6440 7588 7833 1606 Size: 3267 890 677 1148 245 2120 Fragments arranged by size: 3267 2120 1148 890 677 245 NcoI C'CATG_G Cuts at: 513 1065 5571 513 Size: 552 4506 3289 Fragments arranged by size: 4506 3289 552 NdeI CA'TA_TG Cuts at: 387 2781 4806 387 Size: 2394 2025 3928 Fragments arranged by size: 3928 2394 2025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8347 NotI GC'GGCC_GC Cuts at: 5794 5794 Size: 8347 NsiI A_TGCA'T Cuts at: 5005 5005 Size: 8347 NspI r_CATG'y Cuts at: 1132 1229 3973 4924 4999 5007 5705 7250 1132 Size: 97 2744 951 75 8 698 1545 2229 Fragments arranged by size: 2744 2229 1545 951 698 97 75 8 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8347 PciI A'CATG_T Cuts at: 3969 4920 7246 3969 Size: 951 2326 5070 Fragments arranged by size: 5070 2326 951 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8347 PfoI T'CCnGG_A Cuts at: 4337 4337 Size: 8347 PmeI GTTT'AAAC Cuts at: 5651 5651 Size: 8347 PmlI CAC'GTG Cuts at: 2224 2224 Size: 8347 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6416 6448 7929 7961 6416 Size: 32 1481 32 6802 Fragments arranged by size: 6802 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4116 4116 Size: 8347 PshAI GACnn'nnGTC Cuts at: 1613 4956 1613 Size: 3343 5004 PsiI TTA'TAA Cuts at: 5917 5917 Size: 8347 PspXI vC'TCGA_Gb Cuts at: 2771 2771 Size: 8347 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3375 3407 5321 5353 3375 Size: 32 1914 32 6369 Fragments arranged by size: 6369 1914 32 32 PstI C_TGCA'G Cuts at: 830 3849 5699 830 Size: 3019 1850 3478 Fragments arranged by size: 3478 3019 1850 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8347 SacI G_AGCT'C Cuts at: 4437 4437 Size: 8347 SalI G'TCGA_C Cuts at: 1936 5689 1936 Size: 3753 4594 SapI GCTCTTCn'nnn_ Cuts at: 4701 4701 Size: 8347 SbfI CC_TGCA'GG Cuts at: 5699 5699 Size: 8347 ScaI AGT'ACT Cuts at: 4103 6595 8307 4103 Size: 2492 1712 4143 Fragments arranged by size: 4143 2492 1712 SfcI C'TryA_G Cuts at: 826 1045 2322 2740 2977 3395 3845 4233 5695 Size: 219 1277 418 237 418 450 388 1462 Cuts at: 5695 6830 7511 7702 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1462 1277 1135 681 450 418 418 388 237 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8347 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8347 SmaI CCC'GGG Cuts at: 1872 5674 1872 Size: 3802 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2771 3643 5140 6451 7352 7614 Size: 197 926 828 872 1497 1311 901 262 Cuts at: 7614 820 Size: 1553 Fragments arranged by size: 1553 1497 1311 926 901 872 828 262 197 SnaBI TAC'GTA Cuts at: 493 3683 6138 493 Size: 3190 2455 2702 Fragments arranged by size: 3190 2702 2455 SpeI A'CTAG_T Cuts at: 152 3239 152 Size: 3087 5260 SphI G_CATG'C Cuts at: 1132 5705 1132 Size: 4573 3774 SspI AAT'ATT Cuts at: 5 52 3526 4309 6271 5 Size: 47 3474 783 1962 2081 Fragments arranged by size: 3474 2081 1962 783 47 StuI AGG'CCT Cuts at: 1517 2873 4929 1517 Size: 1356 2056 4935 Fragments arranged by size: 4935 2056 1356 StyI C'CwwG_G Cuts at: 513 1065 2560 2827 5571 5786 513 Size: 552 1495 267 2744 215 3074 Fragments arranged by size: 3074 2744 1495 552 267 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2780 3527 6374 6391 6544 6729 7174 Size: 1255 1200 747 2847 17 153 185 445 Cuts at: 7174 325 Size: 1498 Fragments arranged by size: 2847 1498 1255 1200 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3919 3976 4101 Size: 274 80 33 51 1443 1942 57 125 Cuts at: 4101 6593 8305 96 Size: 2492 1712 138 Fragments arranged by size: 2492 1942 1712 1443 274 138 125 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3719 3751 4681 4713 5222 5254 8307 8339 3719 Size: 32 930 32 509 32 3053 32 3727 Fragments arranged by size: 3727 3053 930 509 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8347 XbaI T'CTAG_A Cuts at: 5683 5683 Size: 8347 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2458 3427 2458 Size: 969 7378 XhoI C'TCGA_G Cuts at: 1943 2771 1943 Size: 828 7519 XmaI C'CCGG_G Cuts at: 1870 5672 1870 Size: 3802 4545 XmnI GAAnn'nnTTC Cuts at: 2957 3771 5108 6476 2957 Size: 814 1337 1368 4828 Fragments arranged by size: 4828 1368 1337 814 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6155 276 Size: 53 83 186 936 4621 2468 Fragments arranged by size: 4621 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI