Restriction map for
pFN21AA0461
##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BfrI BlnI
BsaBI BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO109I
EcoO65I EcoRI EcoRV FseI FspI
HgaI Hin1I HpaI KasI KspI
MamI MluI MroI NarI NotI
NruI PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SmaI
Sse8387I SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 415 GGTACC G^GTACC
AccII 2956 CGCG CG^CG
AflIII 1019 ACATGT A^C[GA][CT]GT
Aor51HI 3800 AGCGCT AGC^GCT
Asp718I 415 GGTACC G^GTACC
BsaWI 2441 ACCGGA [AT]^CCGG[AT]
BspHI 1367 TCATGA T^CATGA
BspLU11I 1019 ACATGT A^CATGT
BssSI 2423 CTCGTG CACGAG(-5/-1)
BstBI 551 TTCGAA TT^CGAA
BstUI 2956 CGCG CG^CG
DraI 2257 TTTAAA TTT^AAA
Eco47III 3800 AGCGCT AGC^GCT
EcoNI 3117 CCTACCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 3465 GTCAAC GT[CT]^[GA]AC
HindII 3465 GTCAAC GT[CT]^[GA]AC
KpnI 415 GGTACC GGTAC^C
MfeI 3974 CAATTG C^AATTG
MunI 3974 CAATTG C^AATTG
MvnI 2956 CGCG CG^CG
NcoI 49 CCATGG C^CATGG
NspV 551 TTCGAA TT^CGAA
PshAI 899 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1367 TCATGA T^CATGA
ScaI 2781 AGTACT AGT^ACT
SexAI 759 ACCTGGT A^CC[AT]GGT
SfuI 551 TTCGAA TT^CGAA
SnaBI 2149 TACGTA TAC^GTA
SphI 3512 GCATGC GCATG^C
XbaI 2892 TCTAGA T^CTAGA
XcmI 818 CCATCCCTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AlwNI 1127 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3704 CAGATACTG
AocI 1303 CCTAAGG CC^T[ACGT]AGG
3121 CCTAAGG
AspI 585 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4020 GACCGAGTC
BanII 2235 GAGCCC G[GA]GC[CT]^C
2685 GAGCCC
BclI 2210 TGATCA T^GATCA
2670 TGATCA
BlpI 2524 GCTGAGC GC^T[ACGT]AGC
3249 GCTGAGC
Bpu1102I 2524 GCTGAGC GC^T[ACGT]AGC
3249 GCTGAGC
BsaAI 1387 CACGTA [CT]AC^GT[GA]
2149 TACGTA
BsaI 2983 GAGACC GGTCTC(1/5)
4018 GAGACC
BsgI 2289 CTGCAC GTGCAG(16/14)
2106 GTGCAG
Bsp1407I 327 TGTACA T^GTACA
3162 TGTACA
BspMI 720 ACCTGC ACCTGC(4/8)
2179 ACCTGC
BsrFI 1796 GCCGGC [GA]^CCGG[CT]
2087 GCCGGC
BsrGI 327 TGTACA T^GTACA
3162 TGTACA
Bsu36I 1303 CCTAAGG CC^T[ACGT]AGG
3121 CCTAAGG
CelII 2524 GCTGAGC GC^T[ACGT]AGC
3249 GCTGAGC
Cfr10I 1796 GCCGGC [GA]^CCGG[CT]
2087 GCCGGC
Eco81I 1303 CCTAAGG CC^T[ACGT]AGG
3121 CCTAAGG
EcoT22I 1736 ATGCAT ATGCA^T
3639 ATGCAT
FauI 2610 CCCGC CCCGC(4/6)
2509 GCGGG
FbaI 2210 TGATCA T^GATCA
2670 TGATCA
HaeII 1969 AGCGCC [GA]GCGC^[CT]
3800 AGCGCT
MslI 1896 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3513 CATGCTTGTG
NaeI 1796 GCCGGC GCC^GGC
2087 GCCGGC
NdeI 1375 CATATG CA^TATG
1573 CATATG
NgoMIV 1796 GCCGGC G^CCGGC
2087 GCCGGC
NheI 696 GCTAGC G^CTAGC
2709 GCTAGC
NsiI 1736 ATGCAT ATGCA^T
3639 ATGCAT
PaeR7I 2189 CTCGAG C^TCGAG
3470 CTCGAG
PflFI 585 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4020 GACCGAGTC
SapI 2232 GAAGAGC GCTCTTC(1/4)
2533 GAAGAGC
SpeI 256 ACTAGT A^CTAGT
399 ACTAGT
SspBI 327 TGTACA T^GTACA
3162 TGTACA
SspI 282 AATATT AAT^ATT
1357 AATATT
StuI 220 AGGCCT AGG^CCT
2873 AGGCCT
Tth111I 585 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4020 GACCGAGTC
XhoI 2189 CTCGAG C^TCGAG
3470 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaI AvaII BalI
BanI BbsI BbvI BciVI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI