Restriction map for
pFN21AA0461
(Linear) (Six-base) MAPSORT of: KIAA0461.seq Check: 6906 from: 1 to: 4071
KIAA0461.orf
With 182 enzymes: SgfI *
September 25, 2009 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 729 2188 4071
Size: 729 1459 1883
Fragments arranged by size:
1883 1459 729
Acc65I G'GTAC_C
Cuts at: 0 415 4071
Size: 415 3656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1459 1735 2552 3718 3931 3986 4071
Size: 1022 437 276 817 1166 213 55 85
Fragments arranged by size:
1166 1022 817 437 276 213 85 55
AfeI AGC'GCT
Cuts at: 0 3802 4071
Size: 3802 269
AflIII A'CryG_T
Cuts at: 0 1019 4071
Size: 1019 3052
AleI CACnn'nnGTG
Cuts at: 0 1900 4071
Size: 1900 2171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2392 3529 4071
Size: 2392 1137 542
Fragments arranged by size:
2392 1137 542
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1073 1105 2974 3006 4071
Size: 1073 32 1869 32 1065
Fragments arranged by size:
1869 1073 1065 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1132 3709 4071
Size: 1132 2577 362
Fragments arranged by size:
2577 1132 362
ApoI r'AATT_y
Cuts at: 0 1296 2262 3620 4071
Size: 1296 966 1358 451
Fragments arranged by size:
1358 1296 966 451
AvaI C'yCGr_G
Cuts at: 0 1919 2189 3470 3692 4071
Size: 1919 270 1281 222 379
Fragments arranged by size:
1919 1281 379 270 222
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1172 1205 3230 3263 4071
Size: 1172 33 2025 33 808
Fragments arranged by size:
2025 1172 808 33 33
BanI G'GyrC_C
Cuts at: 0 415 1760 1894 3074 4071
Size: 415 1345 134 1180 997
Fragments arranged by size:
1345 1180 997 415 134
BanII G_rGCy'C
Cuts at: 0 2239 2689 4071
Size: 2239 450 1382
Fragments arranged by size:
2239 1382 450
BbsI GAAGACnn'nnnn_
Cuts at: 0 899 1713 2013 3929 3982 4071
Size: 899 814 300 1916 53 89
Fragments arranged by size:
1916 899 814 300 89 53
BciVI GTATCCnnnnn_n'
Cuts at: 0 1575 1677 1956 3231 4071
Size: 1575 102 279 1275 840
Fragments arranged by size:
1575 1275 840 279 102
BclI T'GATC_A
Cuts at: 0 2210 2670 4071
Size: 2210 460 1401
Fragments arranged by size:
2210 1401 460
BfrBI ATG'CAT
Cuts at: 0 1738 3641 4071
Size: 1738 1903 430
Fragments arranged by size:
1903 1738 430
BglI GCCn_nnn'nGGC
Cuts at: 0 501 3331 3839 4071
Size: 501 2830 508 232
Fragments arranged by size:
2830 508 501 232
BglII A'GATC_T
Cuts at: 0 1529 3038 3221 4056 4071
Size: 1529 1509 183 835 15
Fragments arranged by size:
1529 1509 835 183 15
BlpI GC'TnA_GC
Cuts at: 0 2525 3250 4071
Size: 2525 725 821
Fragments arranged by size:
2525 821 725
Bme1580I G_kGCm'C
Cuts at: 0 196 3295 4071
Size: 196 3099 776
Fragments arranged by size:
3099 776 196
BmrI ACTGGGnnnn_n'
Cuts at: 0 414 567 649 928 1079 2683 4071
Size: 414 153 82 279 151 1604 1388
Fragments arranged by size:
1604 1388 414 279 153 151 82
BmtI G_CTAG'C
Cuts at: 0 700 2713 4071
Size: 700 2013 1358
Fragments arranged by size:
2013 1358 700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 931 1552 1616 1897 2122 4071
Size: 647 284 621 64 281 225 1949
Fragments arranged by size:
1949 647 621 284 281 225 64
Bpu10I CC'TnA_GC
Cuts at: 0 2550 2705 4071
Size: 2550 155 1366
Fragments arranged by size:
2550 1366 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2391 4071
Size: 2391 1680
BsaI GGTCTCn'nnnn_
Cuts at: 0 2977 4012 4071
Size: 2977 1035 59
Fragments arranged by size:
2977 1035 59
BsaAI yAC'GTr
Cuts at: 0 1389 2151 4071
Size: 1389 762 1920
Fragments arranged by size:
1920 1389 762
BsaWI w'CCGG_w
Cuts at: 0 2441 4071
Size: 2441 1630
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 55 1550 1580 2743 2773 2974 3004
Size: 25 30 1495 30 1163 30 201 30
Cuts at: 3004 3448 3478 4071
Size: 444 30 593
Fragments arranged by size:
1495 1163 593 444 201 30 30 30
30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 49 52 1626 1989 2456 2995 3895
Size: 46 3 3 1574 363 467 539 900
Cuts at: 3895 3916 4071
Size: 21 155
Fragments arranged by size:
1574 900 539 467 363 155 46 21
3 3
BseYI C'CCAG_C
Cuts at: 0 578 598 1286 1966 2520 2556 2837 2951
Size: 578 20 688 680 554 36 281 114
Cuts at: 2951 3600 3753 4071
Size: 649 153 318
Fragments arranged by size:
688 680 649 578 554 318 281 153
114 36 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2127 2274 4071
Size: 2127 147 1797
Fragments arranged by size:
2127 1797 147
BsmI GAATG_Cn'
Cuts at: 0 246 1738 3144 3279 4071
Size: 246 1492 1406 135 792
Fragments arranged by size:
1492 1406 792 246 135
Bsp1286I G_dGCh'C
Cuts at: 0 196 2239 2689 3295 4071
Size: 196 2043 450 606 776
Fragments arranged by size:
2043 776 606 450 196
BspHI T'CATG_A
Cuts at: 0 1367 4071
Size: 1367 2704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 729 2188 4071
Size: 729 1459 1883
Fragments arranged by size:
1883 1459 729
BsrDI GCAATG_nn'
Cuts at: 0 344 731 789 1740 4071
Size: 344 387 58 951 2331
Fragments arranged by size:
2331 951 387 344 58
BsrFI r'CCGG_y
Cuts at: 0 1796 2087 4071
Size: 1796 291 1984
Fragments arranged by size:
1984 1796 291
BsrGI T'GTAC_A
Cuts at: 0 327 3162 4071
Size: 327 2835 909
Fragments arranged by size:
2835 909 327
BssSI C'ACGA_G
Cuts at: 0 2423 4071
Size: 2423 1648
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1793 3139 3709 4071
Size: 1793 1346 570 362
Fragments arranged by size:
1793 1346 570 362
BstBI TT'CG_AA
Cuts at: 0 552 4071
Size: 552 3519
BstXI CCAn_nnnn'nTGG
Cuts at: 0 148 841 955 1875 2247 2340 4071
Size: 148 693 114 920 372 93 1731
Fragments arranged by size:
1731 920 693 372 148 114 93
BstYI r'GATC_y
Cuts at: 0 1529 2117 2362 3038 3221 4056 4071
Size: 1529 588 245 676 183 835 15
Fragments arranged by size:
1529 835 676 588 245 183 15
Bsu36I CC'TnA_GG
Cuts at: 0 1304 3122 4071
Size: 1304 1818 949
Fragments arranged by size:
1818 1304 949
BtgI C'CryG_G
Cuts at: 0 49 2227 2380 4071
Size: 49 2178 153 1691
Fragments arranged by size:
2178 1691 153 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1229 2336 3517 4071
Size: 1229 1107 1181 554
Fragments arranged by size:
1229 1181 1107 554
BtsI GCAGTG_nn'
Cuts at: 0 617 731 1782 2154 3261 3599 4071
Size: 617 114 1051 372 1107 338 472
Fragments arranged by size:
1107 1051 617 472 372 338 114
DraI TTT'AAA
Cuts at: 0 2259 4071
Size: 2259 1812
EaeI y'GGCC_r
Cuts at: 0 536 791 2330 2415 4071
Size: 536 255 1539 85 1656
Fragments arranged by size:
1656 1539 536 255 85
EarI CTCTTCn'nnn_
Cuts at: 0 930 1155 1762 2050 2227 2528 2918 3154
Size: 930 225 607 288 177 301 390 236
Cuts at: 3154 3545 3962 4071
Size: 391 417 109
Fragments arranged by size:
930 607 417 391 390 301 288 236
225 177 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 931 1022 1459 1552 1616 1735 1897
Size: 647 284 91 437 93 64 119 162
Cuts at: 1897 2122 2552 3718 3931 3986 4071
Size: 225 430 1166 213 55 85
Fragments arranged by size:
1166 647 437 430 284 225 213 162
119 93 91 85 64 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3121 4071
Size: 3121 950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3532 4071
Size: 3532 539
HaeII r_GCGC'y
Cuts at: 0 1973 3804 4071
Size: 1973 1831 267
Fragments arranged by size:
1973 1831 267
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 159 175 191 672 704 2202 2234
Size: 143 16 16 16 481 32 1498 32
Cuts at: 2234 2698 2730 2743 2775 3208 3240 3448 3480
Size: 464 32 13 32 433 32 208 32
Cuts at: 3480 3513 3545 4009 4041 4071
Size: 33 32 464 32 30
Fragments arranged by size:
1498 481 464 464 433 208 143 33
32 32 32 32 32 32 32 32
30 16 16 16 13
HincII GTy'rAC
Cuts at: 0 3467 4071
Size: 3467 604
HindIII A'AGCT_T
Cuts at: 0 1004 1837 2770 4071
Size: 1004 833 933 1301
Fragments arranged by size:
1301 1004 933 833
KpnI G_GTAC'C
Cuts at: 0 419 4071
Size: 419 3652
MfeI C'AATT_G
Cuts at: 0 3974 4071
Size: 3974 97
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 420 884 1767 4071
Size: 23 397 464 883 2304
Fragments arranged by size:
2304 883 464 397 23
MscI TGG'CCA
Cuts at: 0 793 2332 2417 4071
Size: 793 1539 85 1654
Fragments arranged by size:
1654 1539 793 85
MslI CAynn'nnrTG
Cuts at: 0 1900 3517 4071
Size: 1900 1617 554
Fragments arranged by size:
1900 1617 554
MspA1I CmG'CkG
Cuts at: 0 124 896 1875 1992 2524 2634 2757 2818
Size: 124 772 979 117 532 110 123 61
Cuts at: 2818 3738 4071
Size: 920 333
Fragments arranged by size:
979 920 772 532 333 124 123 117
110 61
NaeI GCC'GGC
Cuts at: 0 1798 2089 4071
Size: 1798 291 1982
Fragments arranged by size:
1982 1798 291
NcoI C'CATG_G
Cuts at: 0 49 4071
Size: 49 4022
NdeI CA'TA_TG
Cuts at: 0 1376 1574 4071
Size: 1376 198 2497
Fragments arranged by size:
2497 1376 198
NgoMIV G'CCGG_C
Cuts at: 0 1796 2087 4071
Size: 1796 291 1984
Fragments arranged by size:
1984 1796 291
NheI G'CTAG_C
Cuts at: 0 696 2709 4071
Size: 696 2013 1362
Fragments arranged by size:
2013 1362 696
NsiI A_TGCA'T
Cuts at: 0 1740 3643 4071
Size: 1740 1903 428
Fragments arranged by size:
1903 1740 428
NspI r_CATG'y
Cuts at: 0 1023 2099 3399 3516 3716 3876 4071
Size: 1023 1076 1300 117 200 160 195
Fragments arranged by size:
1300 1076 1023 200 195 160 117
PasI CC'CwG_GG
Cuts at: 0 828 2880 4071
Size: 828 2052 1191
Fragments arranged by size:
2052 1191 828
PciI A'CATG_T
Cuts at: 0 1019 4071
Size: 1019 3052
PfoI T'CCnGG_A
Cuts at: 0 947 3233 4071
Size: 947 2286 838
Fragments arranged by size:
2286 947 838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2974 3006 4071
Size: 2974 32 1065
Fragments arranged by size:
2974 1065 32
PshAI GACnn'nnGTC
Cuts at: 0 903 4071
Size: 903 3168
PsiI TTA'TAA
Cuts at: 0 98 4071
Size: 98 3973
PspXI vC'TCGA_Gb
Cuts at: 0 3470 4071
Size: 3470 601
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2951 2983 4071
Size: 2951 32 1088
Fragments arranged by size:
2951 1088 32
PstI C_TGCA'G
Cuts at: 0 726 1805 3284 3594 4071
Size: 726 1079 1479 310 477
Fragments arranged by size:
1479 1079 726 477 310
PvuII CAG'CTG
Cuts at: 0 124 1875 2524 2757 2818 3738 4071
Size: 124 1751 649 233 61 920 333
Fragments arranged by size:
1751 920 649 333 233 124 61
SapI GCTCTTCn'nnn_
Cuts at: 0 2227 2528 4071
Size: 2227 301 1543
Fragments arranged by size:
2227 1543 301
ScaI AGT'ACT
Cuts at: 0 2783 4071
Size: 2783 1288
SexAI A'CCwGG_T
Cuts at: 0 759 4071
Size: 759 3312
SfcI C'TryA_G
Cuts at: 0 83 722 1801 2172 2639 3280 3366 3435
Size: 83 639 1079 371 467 641 86 69
Cuts at: 3435 3590 3609 4071
Size: 155 19 462
Fragments arranged by size:
1079 641 639 467 462 371 155 86
83 69 19
SmlI C'TyrA_G
Cuts at: 0 2189 2406 3470 4071
Size: 2189 217 1064 601
Fragments arranged by size:
2189 1064 601 217
SnaBI TAC'GTA
Cuts at: 0 2151 4071
Size: 2151 1920
SpeI A'CTAG_T
Cuts at: 0 256 399 4071
Size: 256 143 3672
Fragments arranged by size:
3672 256 143
SphI G_CATG'C
Cuts at: 0 3516 4071
Size: 3516 555
SspI AAT'ATT
Cuts at: 0 284 1359 4071
Size: 284 1075 2712
Fragments arranged by size:
2712 1075 284
StuI AGG'CCT
Cuts at: 0 222 2875 4071
Size: 222 2653 1196
Fragments arranged by size:
2653 1196 222
StyI C'CwwG_G
Cuts at: 0 49 1817 1898 3304 4071
Size: 49 1768 81 1406 767
Fragments arranged by size:
1768 1406 767 81 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2444 2710 2931 3745 4036 4071
Size: 2444 266 221 814 291 35
Fragments arranged by size:
2444 814 291 266 221 35
TatI w'GTAC_w
Cuts at: 0 327 2781 3162 4071
Size: 327 2454 381 909
Fragments arranged by size:
2454 909 381 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1550 1582 2061 2093 3292 3324 3333 3365
Size: 1550 32 479 32 1199 32 9 32
Cuts at: 3365 4071
Size: 706
Fragments arranged by size:
1550 1199 706 479 32 32 32 32
9
Tth111I GACn'n_nGTC
Cuts at: 0 588 4023 4071
Size: 588 3435 48
Fragments arranged by size:
3435 588 48
XbaI T'CTAG_A
Cuts at: 0 2892 4071
Size: 2892 1179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 825 4071
Size: 825 3246
XhoI C'TCGA_G
Cuts at: 0 2189 3470 4071
Size: 2189 1281 601
Fragments arranged by size:
2189 1281 601
XmnI GAAnn'nnTTC
Cuts at: 0 1493 1720 3989 4071
Size: 1493 227 2269 82
Fragments arranged by size:
2269 1493 227 82
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApoI AvaI BaeI BanI BanII BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI EaeI EarI Eco57MI EcoNI FalI
HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NsiI
NspI PasI PciI PfoI PpiI PshAI PsiI PspXI
PsrI PstI PvuII SapI ScaI SexAI SfcI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI ApaI ApaLI
AscI AseI AsiSI AvrII BamHI BbeI BbvCI BcgI
BmgBI BplI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BssHII BstEII BstZ17I ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI
FspI FspAI HpaI KasI MluI NarI NotI NruI
PacI PflMI PmeI PmlI PpuMI PspOMI PvuI RsrII
SacI SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SrfI SwaI XmaI ZraI