(Circular) (Six-base) MAPSORT of: pFN21AA0433.seq Check: 7080 from: 1 to: 8434 pFN21AA0433 8434 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5569 5569 Size: 8434 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6244 278 Size: 53 83 186 936 4708 2468 Fragments arranged by size: 4708 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5555 5777 1937 Size: 3618 222 4594 Fragments arranged by size: 4594 3618 222 Acc65I G'GTAC_C Cuts at: 4967 5755 4967 Size: 788 7646 AclI AA'CG_TT Cuts at: 1563 4578 6561 6934 1563 Size: 3015 1983 373 3063 Fragments arranged by size: 3063 3015 1983 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2454 2802 2962 3117 3577 3594 3869 3930 Size: 822 348 160 155 460 17 275 61 Cuts at: 3930 4358 6497 7881 8149 1632 Size: 428 2139 1384 268 1917 Fragments arranged by size: 2139 1917 1384 822 460 428 348 275 268 160 155 61 17 AflII C'TTAA_G Cuts at: 820 1017 3389 820 Size: 197 2372 5865 Fragments arranged by size: 5865 2372 197 AflIII A'CryG_T Cuts at: 1203 3856 7333 8310 1203 Size: 2653 3477 977 1327 Fragments arranged by size: 3477 2653 1327 977 AgeI A'CCGG_T Cuts at: 7265 7265 Size: 8434 AhdI GACnn_n'nnGTC Cuts at: 7163 7163 Size: 8434 AleI CACnn'nnGTG Cuts at: 1166 5204 1166 Size: 4038 4396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5222 1184 Size: 4038 4396 AlwNI CAG_nnn'CTG Cuts at: 6177 7749 6177 Size: 1572 6862 ApaLI G'TGCA_C Cuts at: 6491 7647 6491 Size: 1156 7278 ApoI r'AATT_y Cuts at: 1493 2190 2992 3221 4359 4717 5438 5493 5743 Size: 697 802 229 1138 358 721 55 250 Cuts at: 5743 5969 6121 8230 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1138 802 721 697 358 250 229 226 152 55 AseI AT'TA_AT Cuts at: 160 1032 2879 6988 160 Size: 872 1847 4109 1606 Fragments arranged by size: 4109 1847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8434 AvaI C'yCGr_G Cuts at: 1870 1943 5210 5759 1870 Size: 73 3267 549 4545 Fragments arranged by size: 4545 3267 549 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2727 2760 2727 Size: 33 8401 BamHI G'GATC_C Cuts at: 4630 5764 6131 4630 Size: 1134 367 6933 Fragments arranged by size: 6933 1134 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4520 4967 5377 5755 7210 Size: 325 217 627 2733 447 410 378 1455 Cuts at: 7210 618 Size: 1842 Fragments arranged by size: 2733 1842 1455 627 447 410 378 325 217 BanII G_rGCy'C Cuts at: 1391 4593 4937 5753 1391 Size: 3202 344 816 4072 Fragments arranged by size: 4072 3202 816 344 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2100 3992 4716 4916 5017 5489 928 Size: 971 201 1892 724 200 101 472 3873 Fragments arranged by size: 3873 1892 971 724 472 201 200 101 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6625 6659 1903 Size: 34 4688 34 3678 Fragments arranged by size: 4688 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3615 6326 7536 3615 Size: 2711 1210 4513 Fragments arranged by size: 4513 2711 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8434 BfrBI ATG'CAT Cuts at: 3964 3964 Size: 8434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7045 136 Size: 107 122 71 631 605 5373 1525 Fragments arranged by size: 5373 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4901 8429 4901 Size: 3528 4906 BlpI GC'TnA_GC Cuts at: 5851 5851 Size: 8434 Bme1580I G_kGCm'C Cuts at: 1790 6495 7651 1790 Size: 4705 1156 2573 Fragments arranged by size: 4705 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5010 5730 6165 Size: 416 53 83 399 24 3593 720 435 Cuts at: 6165 7123 442 Size: 958 1753 Fragments arranged by size: 3593 1753 958 720 435 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8434 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2658 2781 3630 4340 7094 1239 Size: 1419 123 849 710 2754 2579 Fragments arranged by size: 2754 2579 1419 849 710 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6559 7424 7722 6559 Size: 865 298 7271 Fragments arranged by size: 7271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4765 5181 7097 882 Size: 617 3266 416 1916 2219 Fragments arranged by size: 3266 2219 1916 617 416 BsaAI yAC'GTr Cuts at: 493 1204 4109 5553 6225 493 Size: 711 2905 1444 672 2702 Fragments arranged by size: 2905 2702 1444 711 672 BsaBI GATnn'nnATC Cuts at: 3256 4613 8428 3256 Size: 1357 3815 3262 Fragments arranged by size: 3815 3262 1357 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5005 5295 6241 6623 Size: 53 83 186 936 3472 290 946 382 Cuts at: 6623 7258 275 Size: 635 1451 Fragments arranged by size: 3472 1451 946 936 635 382 290 186 83 53 BsaWI w'CCGG_w Cuts at: 6867 7265 7539 7686 8097 6867 Size: 398 274 147 411 7204 Fragments arranged by size: 7204 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8209 Fragments arranged by size: 8209 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2258 3503 4335 1186 Size: 931 141 1245 832 5285 Fragments arranged by size: 5285 1245 931 832 141 BseYI C'CCAG_C Cuts at: 7637 8133 7637 Size: 496 7938 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2595 5664 1163 Size: 573 859 3069 3933 Fragments arranged by size: 3933 3069 859 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5884 6645 7673 1540 Size: 275 186 3883 761 1028 2301 Fragments arranged by size: 3883 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4593 5211 5603 6495 6580 7651 4593 Size: 618 392 892 85 1071 5376 Fragments arranged by size: 5376 1071 892 618 392 85 BsmI GAATG_Cn' Cuts at: 5945 6038 5945 Size: 93 8341 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4593 4937 5211 5603 5753 6495 6580 Size: 399 2803 344 274 392 150 742 85 Cuts at: 6580 7651 1391 Size: 1071 2174 Fragments arranged by size: 2803 2174 1071 742 399 392 344 274 150 85 BspEI T'CCGG_A Cuts at: 8097 8097 Size: 8434 BspHI T'CATG_A Cuts at: 3874 6323 8053 3874 Size: 2449 1730 4255 Fragments arranged by size: 4255 2449 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5133 5569 5789 844 Size: 4289 436 220 3489 Fragments arranged by size: 4289 3489 436 220 BsrBI CCG'CTC Cuts at: 1394 1823 6321 1394 Size: 429 4498 3507 Fragments arranged by size: 4498 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2689 4537 5451 6929 7103 65 Size: 1183 1441 1848 914 1478 174 1396 Fragments arranged by size: 1848 1478 1441 1396 1183 914 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7078 7265 1700 Size: 110 5268 187 2869 Fragments arranged by size: 5268 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4489 96 Size: 4393 4041 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8434 BssSI C'ACGA_G Cuts at: 2868 6494 7506 2868 Size: 3626 1012 3796 Fragments arranged by size: 3796 3626 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8434 BstBI TT'CG_AA Cuts at: 4259 7238 4259 Size: 2979 5455 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8434 BstXI CCAn_nnnn'nTGG Cuts at: 4214 4993 5062 4214 Size: 779 69 7586 Fragments arranged by size: 7586 779 69 BstYI r'GATC_y Cuts at: 1972 2202 4051 4630 4642 4901 5568 5764 6131 Size: 230 1849 579 12 259 667 196 367 Cuts at: 6131 6516 6533 7985 8071 8083 8429 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1849 1452 667 579 385 367 346 259 230 196 86 17 12 12 BstZ17I GTA'TAC Cuts at: 5556 5556 Size: 8434 Bsu36I CC'TnA_GG Cuts at: 4287 4287 Size: 8434 BtgI C'CryG_G Cuts at: 513 1065 2154 3074 5373 5477 513 Size: 552 1089 920 2299 104 3470 Fragments arranged by size: 3470 2299 1089 920 552 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7695 Fragments arranged by size: 7695 683 56 BtsI GCAGTG_nn' Cuts at: 5950 6744 6764 5950 Size: 794 20 7620 Fragments arranged by size: 7620 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8434 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8399 DraI TTT'AAA Cuts at: 2215 4138 4575 4780 5424 5547 5738 2215 Size: 1923 437 205 644 123 191 4911 Fragments arranged by size: 4911 1923 644 437 205 191 123 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7441 809 Size: 507 6125 1802 Fragments arranged by size: 6125 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5881 6770 8399 Size: 54 1425 50 275 18 4051 889 1629 Cuts at: 8399 8 Size: 43 Fragments arranged by size: 4051 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5881 1537 Size: 275 4069 4090 Fragments arranged by size: 4090 4069 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2235 2709 3480 3496 3580 4923 5281 5409 Size: 882 474 771 16 84 1343 358 128 Cuts at: 5409 6364 1353 Size: 955 3423 Fragments arranged by size: 3423 1343 955 882 771 474 358 128 84 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7021 7395 7541 387 Size: 1064 138 5432 374 146 1280 Fragments arranged by size: 5432 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4591 4591 Size: 8434 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2454 2658 2781 2802 2962 3117 3577 Size: 393 822 204 123 21 160 155 460 Cuts at: 3577 3594 3630 3869 3930 4340 4358 6497 7094 Size: 17 36 239 61 410 18 2139 597 Cuts at: 7094 7881 8149 1239 Size: 787 268 1524 Fragments arranged by size: 2139 1524 822 787 597 460 410 393 268 239 204 160 155 123 61 36 21 18 17 EcoNI CCTnn'n_nnAGG Cuts at: 5169 5169 Size: 8434 EcoRI G'AATT_C Cuts at: 5743 6121 5743 Size: 378 8056 EcoRV GAT'ATC Cuts at: 3103 3103 Size: 8434 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2915 3782 5652 2915 Size: 867 1870 5697 Fragments arranged by size: 5697 1870 867 FspI TGC'GCA Cuts at: 6940 6940 Size: 8434 HaeII r_GCGC'y Cuts at: 1678 7581 1678 Size: 5903 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3397 3429 3666 3698 3818 Size: 32 135 32 1825 32 237 32 120 Cuts at: 3818 3832 3850 3864 4043 4075 4634 4666 7075 Size: 14 18 14 179 32 559 32 2409 Cuts at: 7075 7107 7149 7181 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2409 1825 559 237 179 135 120 42 32 32 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 3447 5778 6024 669 Size: 988 281 1509 2331 246 3079 Fragments arranged by size: 3079 2331 1509 988 281 246 HindIII A'AGCT_T Cuts at: 748 3923 748 Size: 3175 5259 HpaI GTT'AAC Cuts at: 6024 6024 Size: 8434 KpnI G_GTAC'C Cuts at: 4971 5759 4971 Size: 788 7646 MfeI C'AATT_G Cuts at: 3108 6033 3108 Size: 2925 5509 MluI A'CGCG_T Cuts at: 8310 8310 Size: 8434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2011 2122 7548 7732 1305 Size: 706 111 5426 184 2007 Fragments arranged by size: 5426 2007 706 184 111 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6612 Fragments arranged by size: 6612 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2088 5204 6392 6751 6910 518 Size: 648 922 3116 1188 359 159 2042 Fragments arranged by size: 3116 2042 1188 922 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3728 5489 5850 6527 7675 7920 1606 Size: 2122 1761 361 677 1148 245 2120 Fragments arranged by size: 2122 2120 1761 1148 677 361 245 NcoI C'CATG_G Cuts at: 513 1065 2154 3074 5373 513 Size: 552 1089 920 2299 3574 Fragments arranged by size: 3574 2299 1089 920 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8434 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8434 NotI GC'GGCC_GC Cuts at: 5881 5881 Size: 8434 NsiI A_TGCA'T Cuts at: 3966 3966 Size: 8434 NspI r_CATG'y Cuts at: 1132 1229 5792 7337 1132 Size: 97 4563 1545 2229 Fragments arranged by size: 4563 2229 1545 97 PacI TTA_AT'TAA Cuts at: 3972 3972 Size: 8434 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8434 PciI A'CATG_T Cuts at: 7333 7333 Size: 8434 PflMI CCAn_nnn'nTGG Cuts at: 1108 5354 1108 Size: 4246 4188 PfoI T'CCnGG_A Cuts at: 2056 3607 4687 2056 Size: 1551 1080 5803 Fragments arranged by size: 5803 1551 1080 PmeI GTTT'AAAC Cuts at: 5738 5738 Size: 8434 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6503 6535 8016 8048 6503 Size: 32 1481 32 6889 Fragments arranged by size: 6889 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8434 PsiI TTA'TAA Cuts at: 6004 6004 Size: 8434 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3501 3533 3501 Size: 32 8402 PstI C_TGCA'G Cuts at: 830 3559 3727 5786 830 Size: 2729 168 2059 3478 Fragments arranged by size: 3478 2729 2059 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8434 PvuII CAG'CTG Cuts at: 3728 3728 Size: 8434 SacI G_AGCT'C Cuts at: 4593 4593 Size: 8434 SalI G'TCGA_C Cuts at: 1936 5776 1936 Size: 3840 4594 SapI GCTCTTCn'nnn_ Cuts at: 3580 3580 Size: 8434 SbfI CC_TGCA'GG Cuts at: 3559 5786 3559 Size: 2227 6207 ScaI AGT'ACT Cuts at: 6682 8394 6682 Size: 1712 6722 SfcI C'TryA_G Cuts at: 826 1045 3555 3670 3723 5169 5237 5502 5782 Size: 219 2510 115 53 1446 68 265 280 Cuts at: 5782 6917 7598 7789 826 Size: 1135 681 191 1471 Fragments arranged by size: 2510 1471 1446 1135 681 280 265 219 191 115 68 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8434 SmaI CCC'GGG Cuts at: 1872 5761 1872 Size: 3889 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3389 6538 7439 7701 820 Size: 197 926 1446 3149 901 262 1553 Fragments arranged by size: 3149 1553 1446 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4109 5553 6225 493 Size: 3616 1444 672 2702 Fragments arranged by size: 3616 2702 1444 672 SpeI A'CTAG_T Cuts at: 152 152 Size: 8434 SphI G_CATG'C Cuts at: 1132 5792 1132 Size: 4660 3774 SspI AAT'ATT Cuts at: 5 52 3037 6358 5 Size: 47 2985 3321 2081 Fragments arranged by size: 3321 2985 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8434 StyI C'CwwG_G Cuts at: 513 1065 2154 3074 5373 5873 513 Size: 552 1089 920 2299 500 3074 Fragments arranged by size: 3074 2299 1089 920 552 500 SwaI ATTT'AAAT Cuts at: 2215 2215 Size: 8434 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3787 5361 6461 6478 6631 6816 7261 Size: 1255 2207 1574 1100 17 153 185 445 Cuts at: 7261 325 Size: 1498 Fragments arranged by size: 2207 1574 1498 1255 1100 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4340 4489 6680 Size: 274 80 33 51 1443 2363 149 2191 Cuts at: 6680 8392 96 Size: 1712 138 Fragments arranged by size: 2363 2191 1712 1443 274 149 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4535 4567 8394 8426 4535 Size: 32 3827 32 4543 Fragments arranged by size: 4543 3827 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8434 XbaI T'CTAG_A Cuts at: 4424 5395 5770 4424 Size: 971 375 7088 Fragments arranged by size: 7088 971 375 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2271 2271 Size: 8434 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8434 XmaI C'CCGG_G Cuts at: 1870 5759 1870 Size: 3889 4545 XmnI GAAnn'nnTTC Cuts at: 2149 2541 3882 3943 4662 5403 6563 2149 Size: 392 1341 61 719 741 1160 4020 Fragments arranged by size: 4020 1341 1160 741 719 392 61 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6242 276 Size: 53 83 186 936 4708 2468 Fragments arranged by size: 4708 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI