(Circular) (Six-base) MAPSORT of: pFN21AA0374.seq Check: 5166 from: 1 to: 6319 pFN21AA0374 6319 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2161 3662 1937 Size: 224 1501 4594 Fragments arranged by size: 4594 1501 224 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2435 2486 2561 3186 4382 5766 6034 1632 Size: 803 51 75 625 1196 1384 268 1917 Fragments arranged by size: 1917 1384 1196 803 625 268 75 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 3163 5048 3163 Size: 1885 4434 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3087 1184 Size: 1903 4416 AlwNI CAG_nnn'CTG Cuts at: 2362 3161 4062 5634 2362 Size: 799 901 1572 3047 Fragments arranged by size: 3047 1572 901 799 ApaI G_GGCC'C Cuts at: 2066 2990 3210 2066 Size: 924 220 5175 Fragments arranged by size: 5175 924 220 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 3628 3854 4006 6115 1493 Size: 2135 226 152 2109 1697 Fragments arranged by size: 2135 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2093 3644 1870 Size: 73 150 1551 4545 Fragments arranged by size: 4545 1551 150 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2225 2397 2948 3177 3302 Size: 325 217 627 438 172 551 229 125 Cuts at: 3302 3436 3480 3640 5095 618 Size: 134 44 160 1455 1842 Fragments arranged by size: 1842 1455 627 551 438 325 229 217 172 160 134 125 44 BanII G_rGCy'C Cuts at: 1391 2066 2990 3210 3270 3387 3638 1391 Size: 675 924 220 60 117 251 4072 Fragments arranged by size: 4072 924 675 251 220 117 60 BbeI G_GCGC'C Cuts at: 3484 3484 Size: 6319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2474 928 Size: 971 575 4773 Fragments arranged by size: 4773 971 575 BbvCI CC'TCA_GC Cuts at: 2086 2847 2086 Size: 761 5558 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4510 4544 1903 Size: 34 50 34 2489 34 3678 Fragments arranged by size: 3678 2489 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2091 4211 5421 2091 Size: 2120 1210 2989 Fragments arranged by size: 2989 2120 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2534 3600 4930 Size: 107 122 71 631 605 862 1066 1330 Cuts at: 4930 136 Size: 1525 Fragments arranged by size: 1525 1330 1066 862 631 605 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 2237 3575 3736 2237 Size: 1338 161 4820 Fragments arranged by size: 4820 1338 161 Bme1580I G_kGCm'C Cuts at: 1790 2066 2990 3182 3210 3344 3450 4380 5536 Size: 276 924 192 28 134 106 930 1156 Cuts at: 5536 1790 Size: 2573 Fragments arranged by size: 2573 1156 930 924 276 192 134 106 28 BmgBI CAC'GTC Cuts at: 1318 2037 1318 Size: 719 5600 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2736 4050 5008 Size: 416 53 83 399 24 1319 1314 958 Cuts at: 5008 442 Size: 1753 Fragments arranged by size: 1753 1319 1314 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2977 2977 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2687 2818 3143 3418 4979 1239 Size: 1181 267 131 325 275 1561 2579 Fragments arranged by size: 2579 1561 1181 325 275 267 131 Bpu10I CC'TnA_GC Cuts at: 2086 2847 2086 Size: 761 5558 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2555 4444 5309 5607 2555 Size: 1889 865 298 3267 Fragments arranged by size: 3267 1889 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2951 3207 4982 882 Size: 617 1452 256 1775 2219 Fragments arranged by size: 2219 1775 1452 617 256 BsaAI yAC'GTr Cuts at: 493 1204 4110 493 Size: 711 2906 2702 Fragments arranged by size: 2906 2702 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3481 4126 4508 5143 Size: 53 83 186 936 1948 645 382 635 Cuts at: 5143 275 Size: 1451 Fragments arranged by size: 1948 1451 936 645 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4752 5150 5424 5571 5982 4752 Size: 398 274 147 411 5089 Fragments arranged by size: 5089 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3494 3524 1178 Size: 30 165 30 2091 30 3973 Fragments arranged by size: 3973 2091 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2074 2519 2522 2969 3368 3371 3374 1186 Size: 888 445 3 447 399 3 3 4131 Fragments arranged by size: 4131 888 447 445 399 3 3 3 BseYI C'CCAG_C Cuts at: 2021 2529 3474 3590 3595 3612 5522 6018 2021 Size: 508 945 116 5 17 1910 496 2322 Fragments arranged by size: 2322 1910 945 508 496 116 17 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2338 2663 2951 3068 3113 3514 1163 Size: 573 602 325 288 117 45 401 3968 Fragments arranged by size: 3968 602 573 401 325 288 117 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3444 3769 4530 5558 1540 Size: 275 186 1443 325 761 1028 2301 Fragments arranged by size: 2301 1443 1028 761 325 275 186 BsiHKAI G_wGCw'C Cuts at: 4380 4465 5536 4380 Size: 85 1071 5163 Fragments arranged by size: 5163 1071 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 BsmBI CGTCTCn'nnnn_ Cuts at: 2663 2663 Size: 6319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2990 3182 3210 3270 3344 3387 Size: 399 276 924 192 28 60 74 43 Cuts at: 3387 3450 3638 4380 4465 5536 1391 Size: 63 188 742 85 1071 2174 Fragments arranged by size: 2174 1071 924 742 399 276 192 188 85 74 63 60 43 28 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2566 3674 844 Size: 1722 1108 3489 Fragments arranged by size: 3489 1722 1108 BsrBI CCG'CTC Cuts at: 1394 1823 2757 3471 3570 4206 1394 Size: 429 934 714 99 636 3507 Fragments arranged by size: 3507 934 714 636 429 99 BsrDI GCAATG_nn' Cuts at: 65 1248 4814 4988 65 Size: 1183 3566 174 1396 Fragments arranged by size: 3566 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2430 2738 3439 4963 5150 1700 Size: 110 620 308 701 1524 187 2869 Fragments arranged by size: 2869 1524 701 620 308 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6319 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 2810 1184 Size: 1626 4693 BstXI CCAn_nnnn'nTGG Cuts at: 3149 3149 Size: 6319 BstYI r'GATC_y Cuts at: 1972 3418 3649 4016 4401 4418 5870 5956 5968 Size: 1446 231 367 385 17 1452 86 12 Cuts at: 5968 6314 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1446 385 367 346 231 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2329 3277 3345 3369 3450 513 Size: 552 1264 948 68 24 81 3382 Fragments arranged by size: 3382 1264 948 552 81 68 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 2278 2326 3234 3835 4629 4649 2278 Size: 48 908 601 794 20 3948 Fragments arranged by size: 3948 908 794 601 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DraIII CAC_nnn'GTG Cuts at: 2316 2521 2316 Size: 205 6114 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2476 2801 5326 809 Size: 507 1160 325 2525 1802 Fragments arranged by size: 2525 1802 1160 507 325 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2709 3319 Size: 54 1425 50 275 18 309 570 610 Cuts at: 3319 3441 3766 4655 6284 8 Size: 122 325 889 1629 43 Fragments arranged by size: 1629 1425 889 610 570 325 309 275 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3441 3766 1537 Size: 275 1629 325 4090 Fragments arranged by size: 4090 1629 325 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2236 3345 4249 1353 Size: 883 1109 904 3423 Fragments arranged by size: 3423 1109 904 883 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2435 2486 2561 2687 2818 3143 Size: 393 788 15 51 75 126 131 325 Cuts at: 3143 3186 3418 4382 4979 5766 6034 1239 Size: 43 232 964 597 787 268 1524 Fragments arranged by size: 1524 964 788 787 597 393 325 268 232 131 126 75 51 43 15 EcoNI CCTnn'n_nnAGG Cuts at: 2543 3165 2543 Size: 622 5697 EcoO109I rG'GnC_Cy Cuts at: 2063 2745 2987 3206 3259 2063 Size: 682 242 219 53 5123 Fragments arranged by size: 5123 682 242 219 53 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2299 3484 5466 1678 Size: 621 1185 1982 2531 Fragments arranged by size: 2531 1982 1185 621 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3149 3181 3206 3238 4960 Size: 32 135 32 1577 32 25 32 1722 Cuts at: 4960 4992 5034 5066 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1722 1577 135 42 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KasI G'GCGC_C Cuts at: 3480 3480 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 2711 3321 10 Size: 54 1425 343 309 570 610 3008 Fragments arranged by size: 3008 1425 610 570 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2690 4277 4636 4795 518 Size: 648 975 549 1587 359 159 2042 Fragments arranged by size: 2042 1587 975 648 549 359 159 MspA1I CmG'CkG Cuts at: 1606 2016 2476 2533 2838 3057 3295 3478 3564 Size: 410 460 57 305 219 238 183 86 Cuts at: 3564 3599 3735 4412 5560 5805 1606 Size: 35 136 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 460 410 305 245 238 219 183 136 86 57 35 NaeI GCC'GGC Cuts at: 3441 3441 Size: 6319 NarI GG'CG_CC Cuts at: 3481 3481 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2329 3345 513 Size: 552 1264 1016 3487 Fragments arranged by size: 3487 1264 1016 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 3439 3439 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2488 3064 3677 5222 1132 Size: 97 1259 576 613 1545 2229 Fragments arranged by size: 2229 1545 1259 613 576 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 2521 1108 Size: 1413 4906 PfoI T'CCnGG_A Cuts at: 2436 3120 2436 Size: 684 5635 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2745 2745 Size: 6319 PshAI GACnn'nnGTC Cuts at: 1613 2280 1613 Size: 667 5652 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspOMI G'GGCC_C Cuts at: 2062 2986 3206 2062 Size: 924 220 5175 Fragments arranged by size: 5175 924 220 PstI C_TGCA'G Cuts at: 830 2376 2628 3671 830 Size: 1546 252 1043 3478 Fragments arranged by size: 3478 1546 1043 252 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 PvuII CAG'CTG Cuts at: 2476 2533 3057 3564 2476 Size: 57 524 507 5231 Fragments arranged by size: 5231 524 507 57 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SanDI GG'GwC_CC Cuts at: 2745 2745 Size: 6319 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 2633 4567 6279 2633 Size: 1934 1712 2673 Fragments arranged by size: 2673 1934 1712 SfcI C'TryA_G Cuts at: 826 1045 2372 2624 3667 4802 5483 5674 826 Size: 219 1327 252 1043 1135 681 191 1471 Fragments arranged by size: 1471 1327 1135 1043 681 252 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2534 2534 Size: 6319 SfoI GGC'GCC Cuts at: 3482 3482 Size: 6319 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 2095 3646 1872 Size: 223 1551 4545 Fragments arranged by size: 4545 1551 223 SmlI C'TyrA_G Cuts at: 820 1017 1943 2570 4423 5324 5586 820 Size: 197 926 627 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 627 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 2488 3064 3677 1132 Size: 1356 576 613 3774 Fragments arranged by size: 3774 1356 613 576 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2291 2329 2712 3345 3758 513 Size: 552 1226 38 383 633 413 3074 Fragments arranged by size: 3074 1226 633 552 413 383 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3233 4346 4363 4516 4701 5146 325 Size: 1255 1653 1113 17 153 185 445 1498 Fragments arranged by size: 1653 1498 1255 1113 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2631 4565 6277 Size: 274 80 33 51 1443 654 1934 1712 Cuts at: 6277 96 Size: 138 Fragments arranged by size: 1934 1712 1443 654 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 2588 1316 Size: 1272 5047 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3226 3226 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 2093 3644 1870 Size: 223 1551 4545 Fragments arranged by size: 4545 1551 223 XmnI GAAnn'nnTTC Cuts at: 2633 4448 2633 Size: 1815 4504 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SrfI SwaI