(Circular) (Six-base) MAPSORT of: pFN21AA0291.seq Check: 5041 from: 1 to: 7738 pFN21AA0291 7738 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4906 4906 Size: 7738 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5548 278 Size: 53 83 186 936 4012 2468 Fragments arranged by size: 4012 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5081 1937 Size: 3144 4594 Acc65I G'GTAC_C Cuts at: 5059 5059 Size: 7738 AclI AA'CG_TT Cuts at: 1563 5865 6238 1563 Size: 4302 373 3063 Fragments arranged by size: 4302 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 3107 3632 3704 3866 4532 5801 7185 Size: 413 1062 525 72 162 666 1269 1384 Cuts at: 7185 7453 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1269 1062 666 525 413 268 162 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7541 AflIII A'CryG_T Cuts at: 1203 6637 7614 1203 Size: 5434 977 1327 Fragments arranged by size: 5434 1327 977 AgeI A'CCGG_T Cuts at: 6569 6569 Size: 7738 AhdI GACnn_n'nnGTC Cuts at: 2943 6467 2943 Size: 3524 4214 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7738 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3035 3240 1184 Size: 1851 205 5682 Fragments arranged by size: 5682 1851 205 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3326 3358 3326 Size: 32 7706 AlwNI CAG_nnn'CTG Cuts at: 2180 2296 2509 3118 3475 4603 5481 7053 2180 Size: 116 213 609 357 1128 878 1572 2865 Fragments arranged by size: 2865 1572 1128 878 609 357 213 116 ApaI G_GGCC'C Cuts at: 2201 3815 4015 4453 2201 Size: 1614 200 438 5486 Fragments arranged by size: 5486 1614 438 200 ApaLI G'TGCA_C Cuts at: 4094 5795 6951 4094 Size: 1701 1156 4881 Fragments arranged by size: 4881 1701 1156 ApoI r'AATT_y Cuts at: 1493 5047 5273 5425 7534 1493 Size: 3554 226 152 2109 1697 Fragments arranged by size: 3554 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6292 160 Size: 872 5260 1606 Fragments arranged by size: 5260 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7738 AvaI C'yCGr_G Cuts at: 1870 1943 2186 2464 3058 3484 3808 4573 4841 Size: 73 243 278 594 426 324 765 268 Cuts at: 4841 5063 1870 Size: 222 4545 Fragments arranged by size: 4545 765 594 426 324 278 268 243 222 73 BamHI G'GATC_C Cuts at: 2612 5068 5435 2612 Size: 2456 367 4915 Fragments arranged by size: 4915 2456 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2801 3062 3818 4524 4837 Size: 325 217 627 1014 261 756 706 313 Cuts at: 4837 5059 6514 618 Size: 222 1455 1842 Fragments arranged by size: 1842 1455 1014 756 706 627 325 313 261 222 217 BanII G_rGCy'C Cuts at: 1391 2201 2465 2772 3256 3435 3465 3815 4015 Size: 810 264 307 484 179 30 350 200 Cuts at: 4015 4453 5057 1391 Size: 438 604 4072 Fragments arranged by size: 4072 810 604 484 438 350 307 264 200 179 30 BbeI G_GCGC'C Cuts at: 3822 4528 4841 3822 Size: 706 313 6719 Fragments arranged by size: 6719 706 313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2600 2702 2909 4565 928 Size: 971 701 102 207 1656 4101 Fragments arranged by size: 4101 1656 971 701 207 102 BbvCI CC'TCA_GC Cuts at: 3144 3144 Size: 7738 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5929 5963 1903 Size: 34 50 34 3908 34 3678 Fragments arranged by size: 3908 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5630 6840 5630 Size: 1210 6528 BclI T'GATC_A Cuts at: 1548 1548 Size: 7738 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2064 3015 3614 Size: 107 122 71 631 605 392 951 599 Cuts at: 3614 3623 6349 136 Size: 9 2726 1525 Fragments arranged by size: 2726 1525 951 631 605 599 392 122 107 71 9 BglII A'GATC_T Cuts at: 3054 4164 7733 3054 Size: 1110 3569 3059 Fragments arranged by size: 3569 3059 1110 BlpI GC'TnA_GC Cuts at: 4911 5155 4911 Size: 244 7494 Bme1580I G_kGCm'C Cuts at: 1790 2201 2402 2528 2804 2822 3065 3815 4015 Size: 411 201 126 276 18 243 750 200 Cuts at: 4015 4098 4453 5799 6955 1790 Size: 83 355 1346 1156 2573 Fragments arranged by size: 2573 1346 1156 750 411 355 276 243 201 200 126 83 18 BmgBI CAC'GTC Cuts at: 1318 4117 1318 Size: 2799 4939 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2088 5469 6427 Size: 416 53 83 399 24 671 3381 958 Cuts at: 6427 442 Size: 1753 Fragments arranged by size: 3381 1753 958 671 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2115 1056 Size: 1059 6679 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4183 4183 Size: 7738 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2038 2393 3329 3401 3443 3449 3803 3905 Size: 799 355 936 72 42 6 354 102 Cuts at: 3905 3938 4040 4067 4265 4487 4550 4562 4670 Size: 33 102 27 198 222 63 12 108 Cuts at: 4670 6398 1239 Size: 1728 2579 Fragments arranged by size: 2579 1728 936 799 355 354 222 198 108 102 102 72 63 42 33 27 12 6 Bpu10I CC'TnA_GC Cuts at: 3144 4445 4688 3144 Size: 1301 243 6194 Fragments arranged by size: 6194 1301 243 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3185 4139 4721 5863 6728 7026 3185 Size: 954 582 1142 865 298 3897 Fragments arranged by size: 3897 1142 954 865 582 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2840 3023 3123 3369 3914 4538 6401 Size: 617 1341 183 100 246 545 624 1863 Cuts at: 6401 882 Size: 2219 Fragments arranged by size: 2219 1863 1341 624 617 545 246 183 100 BsaAI yAC'GTr Cuts at: 493 1204 2716 3934 5529 493 Size: 711 1512 1218 1595 2702 Fragments arranged by size: 2702 1595 1512 1218 711 BsaBI GATnn'nnATC Cuts at: 2864 7732 2864 Size: 4868 2870 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3513 3819 4503 4525 Size: 53 83 186 936 1980 306 684 22 Cuts at: 4525 4569 4838 4884 5545 5927 6562 275 Size: 44 269 46 661 382 635 1451 Fragments arranged by size: 1980 1451 936 684 661 635 382 306 269 186 83 53 46 44 22 BsaWI w'CCGG_w Cuts at: 3499 4979 6171 6569 6843 6990 7401 3499 Size: 1480 1192 398 274 147 411 3836 Fragments arranged by size: 3836 1480 1192 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3344 3374 4460 4490 4535 Size: 30 165 30 1941 30 1086 30 45 Cuts at: 4535 4565 1178 Size: 30 4351 Fragments arranged by size: 4351 1941 1086 165 45 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2149 2152 2977 3251 3326 3329 3334 3368 Size: 963 3 825 274 75 3 5 34 Cuts at: 3368 3371 3440 3443 3473 3995 4294 4361 4957 Size: 3 69 3 30 522 299 67 596 Cuts at: 4957 1186 Size: 3967 Fragments arranged by size: 3967 963 825 596 522 299 274 75 69 67 34 30 5 3 3 3 3 BseYI C'CCAG_C Cuts at: 2015 2887 2948 3005 3822 3889 3989 4014 4308 Size: 872 61 57 817 67 100 25 294 Cuts at: 4308 6941 7437 2015 Size: 2633 496 2316 Fragments arranged by size: 2633 2316 872 817 496 294 100 67 61 57 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2120 2309 1163 Size: 573 384 189 6592 Fragments arranged by size: 6592 573 384 189 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 2172 2457 4290 5188 5949 Size: 275 186 37 134 285 1833 898 761 Cuts at: 5949 6977 1540 Size: 1028 2301 Fragments arranged by size: 2301 1833 1028 898 761 285 275 186 134 37 BsiHKAI G_wGCw'C Cuts at: 3435 3465 3879 4098 4304 4877 5799 5884 6955 Size: 30 414 219 206 573 922 85 1071 Cuts at: 6955 3435 Size: 4218 Fragments arranged by size: 4218 1071 922 573 414 219 206 85 30 BsmI GAATG_Cn' Cuts at: 2518 5249 5342 2518 Size: 2731 93 4914 Fragments arranged by size: 4914 2731 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2297 4343 2297 Size: 2046 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2402 2465 2528 2772 2804 2822 Size: 399 411 201 63 63 244 32 18 Cuts at: 2822 3065 3256 3435 3465 3815 3879 4015 4098 Size: 243 191 179 30 350 64 136 83 Cuts at: 4098 4304 4453 4877 5057 5799 5884 6955 1391 Size: 206 149 424 180 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 411 399 350 244 243 206 201 191 180 179 149 136 85 83 64 63 63 32 30 18 BspEI T'CCGG_A Cuts at: 3499 7401 3499 Size: 3902 3836 BspHI T'CATG_A Cuts at: 5627 7357 5627 Size: 1730 6008 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2667 4906 5093 844 Size: 1823 2239 187 3489 Fragments arranged by size: 3489 2239 1823 187 BsrBI CCG'CTC Cuts at: 1394 1823 2254 3541 4201 4618 5625 1394 Size: 429 431 1287 660 417 1007 3507 Fragments arranged by size: 3507 1287 1007 660 431 429 417 BsrDI GCAATG_nn' Cuts at: 65 1248 2513 6233 6407 65 Size: 1183 1265 3720 174 1396 Fragments arranged by size: 3720 1396 1265 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2597 4041 6382 6569 1700 Size: 110 787 1444 2341 187 2869 Fragments arranged by size: 2869 2341 1444 787 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7738 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7738 BssSI C'ACGA_G Cuts at: 3263 4097 5798 6810 3263 Size: 834 1701 1012 4191 Fragments arranged by size: 4191 1701 1012 834 BstAPI GCAn_nnn'nTGC Cuts at: 794 2180 794 Size: 1386 6352 BstBI TT'CG_AA Cuts at: 6542 6542 Size: 7738 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7738 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2330 2914 3307 2062 Size: 268 584 393 6493 Fragments arranged by size: 6493 584 393 268 BstYI r'GATC_y Cuts at: 1972 2612 3054 3334 3522 4164 5068 5435 5820 Size: 640 442 280 188 642 904 367 385 Cuts at: 5820 5837 7289 7375 7387 7733 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 904 642 640 442 385 367 346 280 188 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2038 2061 2451 2484 2919 2991 3066 Size: 552 973 23 390 33 435 72 75 Cuts at: 3066 513 Size: 5185 Fragments arranged by size: 5185 973 552 435 390 75 72 33 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6999 Fragments arranged by size: 6999 683 56 BtsI GCAGTG_nn' Cuts at: 2132 3019 3476 3858 5254 6048 6068 2132 Size: 887 457 382 1396 794 20 3802 Fragments arranged by size: 3802 1396 887 794 457 382 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7738 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7703 DraI TTT'AAA Cuts at: 5042 5042 Size: 7738 DraIII CAC_nnn'GTG Cuts at: 2485 2857 2485 Size: 372 7366 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6745 809 Size: 507 5429 1802 Fragments arranged by size: 5429 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2106 2169 Size: 54 1425 50 275 18 205 71 63 Cuts at: 2169 2454 2529 2916 3150 3396 3552 3648 4173 Size: 285 75 387 234 246 156 96 525 Cuts at: 4173 5185 6074 7703 8 Size: 1012 889 1629 43 Fragments arranged by size: 1629 1425 1012 889 525 387 285 275 246 234 205 156 96 75 71 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2035 2169 2454 5185 1537 Size: 275 223 134 285 2731 4090 Fragments arranged by size: 4090 2731 285 275 223 134 EarI CTCTTCn'nnn_ Cuts at: 1353 2843 3040 5668 1353 Size: 1490 197 2628 3423 Fragments arranged by size: 3423 2628 1490 197 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2225 3641 4732 6325 6699 6845 Size: 1064 138 636 1416 1091 1593 374 146 Cuts at: 6845 387 Size: 1280 Fragments arranged by size: 1593 1416 1280 1091 1064 636 374 146 138 EcoICRI GAG'CTC Cuts at: 3433 3463 3433 Size: 30 7708 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2045 2393 3107 3329 3401 3443 Size: 393 406 7 348 714 222 72 42 Cuts at: 3443 3449 3632 3704 3803 3866 3905 3938 4040 Size: 6 183 72 99 63 39 33 102 Cuts at: 4040 4067 4265 4487 4532 4550 4562 4670 5801 Size: 27 198 222 45 18 12 108 1131 Cuts at: 5801 6398 7185 7453 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1131 787 714 597 406 393 348 268 222 222 198 183 108 102 99 72 72 63 45 42 39 33 27 18 12 7 6 EcoNI CCTnn'n_nnAGG Cuts at: 3200 4511 3200 Size: 1311 6427 EcoO109I rG'GnC_Cy Cuts at: 2198 2656 3488 3607 3787 3949 4294 4378 4449 Size: 458 832 119 180 162 345 84 71 Cuts at: 4449 4684 4753 2198 Size: 235 69 5183 Fragments arranged by size: 5183 832 458 345 235 180 162 119 84 71 69 EcoRI G'AATT_C Cuts at: 5047 5425 5047 Size: 378 7360 FspI TGC'GCA Cuts at: 6244 6244 Size: 7738 HaeII r_GCGC'y Cuts at: 1678 3822 4075 4528 4813 4841 6885 1678 Size: 2144 253 453 285 28 2044 2531 Fragments arranged by size: 2531 2144 2044 453 285 253 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2465 2497 2597 2604 2618 Size: 32 135 32 893 32 100 7 14 Cuts at: 2618 2629 2636 2650 2929 2961 3344 3376 3438 Size: 11 7 14 279 32 383 32 62 Cuts at: 3438 3470 4535 4567 4817 4849 6379 6411 6453 Size: 32 1065 32 250 32 1530 32 42 Cuts at: 6453 6485 1373 Size: 32 2626 Fragments arranged by size: 2626 1530 1065 893 383 279 250 135 100 62 42 32 32 32 32 32 32 32 32 32 32 14 14 11 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 5082 5328 669 Size: 988 281 3144 246 3079 Fragments arranged by size: 3144 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7738 HpaI GTT'AAC Cuts at: 5328 5328 Size: 7738 KasI G'GCGC_C Cuts at: 3818 4524 4837 3818 Size: 706 313 6719 Fragments arranged by size: 6719 706 313 KpnI G_GTAC'C Cuts at: 5063 5063 Size: 7738 MfeI C'AATT_G Cuts at: 5337 5337 Size: 7738 MluI A'CGCG_T Cuts at: 7614 7614 Size: 7738 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2640 3324 4170 4779 6852 7036 1305 Size: 1335 684 846 609 2073 184 2007 Fragments arranged by size: 2073 2007 1335 846 684 609 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2918 3152 10 Size: 54 1425 343 1086 234 4596 Fragments arranged by size: 4596 1425 1086 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2924 3869 3932 4319 5696 6055 6214 Size: 648 1758 945 63 387 1377 359 159 Cuts at: 6214 518 Size: 2042 Fragments arranged by size: 2042 1758 1377 945 648 387 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2019 2177 2542 2631 3115 3307 3421 3883 Size: 413 158 365 89 484 192 114 462 Cuts at: 3883 4000 4018 4210 4867 5154 5831 6979 7224 Size: 117 18 192 657 287 677 1148 245 Cuts at: 7224 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 657 484 462 413 365 287 245 192 192 158 117 114 89 18 NarI GG'CG_CC Cuts at: 3819 4525 4838 3819 Size: 706 313 6719 Fragments arranged by size: 6719 706 313 NcoI C'CATG_G Cuts at: 513 1065 2061 2919 513 Size: 552 996 858 5332 Fragments arranged by size: 5332 996 858 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7738 NheI G'CTAG_C Cuts at: 1052 2111 1052 Size: 1059 6679 NotI GC'GGCC_GC Cuts at: 5185 5185 Size: 7738 NspI r_CATG'y Cuts at: 1132 1229 5096 6641 1132 Size: 97 3867 1545 2229 Fragments arranged by size: 3867 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4222 4670 1348 Size: 2874 448 4416 Fragments arranged by size: 4416 2874 448 PciI A'CATG_T Cuts at: 6637 6637 Size: 7738 PflMI CCAn_nnn'nTGG Cuts at: 1108 2485 1108 Size: 1377 6361 PfoI T'CCnGG_A Cuts at: 4553 4553 Size: 7738 PmeI GTTT'AAAC Cuts at: 5042 5042 Size: 7738 PmlI CAC'GTG Cuts at: 3934 3934 Size: 7738 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2294 2326 5807 5839 7320 7352 2294 Size: 32 3481 32 1481 32 2680 Fragments arranged by size: 3481 2680 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2656 3488 3787 4684 2656 Size: 832 299 897 5710 Fragments arranged by size: 5710 897 832 299 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7738 PsiI TTA'TAA Cuts at: 5308 5308 Size: 7738 PspOMI G'GGCC_C Cuts at: 2197 3811 4011 4449 2197 Size: 1614 200 438 5486 Fragments arranged by size: 5486 1614 438 200 PstI C_TGCA'G Cuts at: 830 3249 3342 3420 3969 3999 4056 4209 4386 Size: 2419 93 78 549 30 57 153 177 Cuts at: 4386 4860 5090 830 Size: 474 230 3478 Fragments arranged by size: 3478 2419 549 474 230 177 153 93 78 57 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7738 PvuII CAG'CTG Cuts at: 2177 3115 3307 3421 3883 4018 4867 2177 Size: 938 192 114 462 135 849 5048 Fragments arranged by size: 5048 938 849 462 192 135 114 SacI G_AGCT'C Cuts at: 3435 3465 3435 Size: 30 7708 SalI G'TCGA_C Cuts at: 1936 5080 1936 Size: 3144 4594 SanDI GG'GwC_CC Cuts at: 3488 3488 Size: 7738 SbfI CC_TGCA'GG Cuts at: 4386 5090 4386 Size: 704 7034 ScaI AGT'ACT Cuts at: 5986 7698 5986 Size: 1712 6026 SexAI A'CCwGG_T Cuts at: 2804 2804 Size: 7738 SfcI C'TryA_G Cuts at: 826 1045 3245 3338 3416 3965 3995 4052 4205 Size: 219 2200 93 78 549 30 57 153 Cuts at: 4205 4382 4856 5086 6221 6902 7093 826 Size: 177 474 230 1135 681 191 1471 Fragments arranged by size: 2200 1471 1135 681 549 474 230 219 191 177 153 93 78 57 30 SfiI GGCCn_nnn'nGGCC Cuts at: 3614 3614 Size: 7738 SfoI GGC'GCC Cuts at: 3820 4526 4839 3820 Size: 706 313 6719 Fragments arranged by size: 6719 706 313 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7738 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7738 SmaI CCC'GGG Cuts at: 1872 5065 1872 Size: 3193 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3200 4154 4736 5842 6743 7005 Size: 197 926 1257 954 582 1106 901 262 Cuts at: 7005 820 Size: 1553 Fragments arranged by size: 1553 1257 1106 954 926 901 582 262 197 SnaBI TAC'GTA Cuts at: 493 5529 493 Size: 5036 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7738 SphI G_CATG'C Cuts at: 1132 5096 1132 Size: 3964 3774 SspI AAT'ATT Cuts at: 5 52 5662 5 Size: 47 5610 2081 Fragments arranged by size: 5610 2081 47 StuI AGG'CCT Cuts at: 1517 3617 1517 Size: 2100 5638 StyI C'CwwG_G Cuts at: 513 1065 2061 2118 2202 2736 2773 2892 2919 Size: 552 996 57 84 534 37 119 27 Cuts at: 2919 3153 3369 5177 513 Size: 234 216 1808 3074 Fragments arranged by size: 3074 1808 996 552 534 234 216 119 84 57 37 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2251 2839 2912 3522 4331 4451 5765 Size: 1255 671 588 73 610 809 120 1314 Cuts at: 5765 5782 5935 6120 6565 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1314 1255 809 671 610 588 445 185 153 120 73 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5984 7696 96 Size: 274 80 33 51 1443 4007 1712 138 Fragments arranged by size: 4007 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3761 3793 4460 4492 7698 7730 3761 Size: 32 667 32 3206 32 3769 Fragments arranged by size: 3769 3206 667 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4549 1316 Size: 3233 4505 XbaI T'CTAG_A Cuts at: 5074 5074 Size: 7738 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3376 3575 3715 3913 3376 Size: 199 140 198 7201 Fragments arranged by size: 7201 199 198 140 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7738 XmaI C'CCGG_G Cuts at: 1870 5063 1870 Size: 3193 4545 XmnI GAAnn'nnTTC Cuts at: 5867 5867 Size: 7738 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5546 276 Size: 53 83 186 936 4012 2468 Fragments arranged by size: 4012 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SapI SrfI SwaI