(Circular) (Six-base) MAPSORT of: pFN21AA0209.seq Check: 4267 from: 1 to: 10195 pFN21AA0209 10195 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4369 4484 4369 Size: 115 10080 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8005 278 Size: 53 83 186 936 6469 2468 Fragments arranged by size: 6469 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6363 7538 1937 Size: 4426 1175 4594 Fragments arranged by size: 4594 4426 1175 Acc65I G'GTAC_C Cuts at: 5670 7516 5670 Size: 1846 8349 AclI AA'CG_TT Cuts at: 1563 8322 8695 1563 Size: 6759 373 3063 Fragments arranged by size: 6759 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 2456 2852 3887 4175 4235 4619 5426 Size: 815 9 396 1035 288 60 384 807 Cuts at: 5426 6681 7184 7473 8258 9642 9910 1632 Size: 1255 503 289 785 1384 268 1917 Fragments arranged by size: 1917 1384 1255 1035 815 807 785 503 396 384 289 288 268 60 9 AflII C'TTAA_G Cuts at: 820 1017 2924 820 Size: 197 1907 8091 Fragments arranged by size: 8091 1907 197 AflIII A'CryG_T Cuts at: 1203 2123 4953 6462 9094 10071 1203 Size: 920 2830 1509 2632 977 1327 Fragments arranged by size: 2830 2632 1509 1327 977 920 AgeI A'CCGG_T Cuts at: 9026 9026 Size: 10195 AhdI GACnn_n'nnGTC Cuts at: 7480 8924 7480 Size: 1444 8751 AleI CACnn'nnGTG Cuts at: 1166 3221 3818 1166 Size: 2055 597 7543 Fragments arranged by size: 7543 2055 597 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2962 3398 4134 1184 Size: 1778 436 736 7245 Fragments arranged by size: 7245 1778 736 436 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4217 4249 4217 Size: 32 10163 AlwNI CAG_nnn'CTG Cuts at: 2959 4642 4750 5461 5723 5968 6284 7938 9510 Size: 1683 108 711 262 245 316 1654 1572 Cuts at: 9510 2959 Size: 3644 Fragments arranged by size: 3644 1683 1654 1572 711 316 262 245 108 ApaLI G'TGCA_C Cuts at: 2947 6458 8252 9408 2947 Size: 3511 1794 1156 3734 Fragments arranged by size: 3734 3511 1794 1156 ApoI r'AATT_y Cuts at: 1493 2256 2880 3901 4067 5629 5984 6958 7504 Size: 763 624 1021 166 1562 355 974 546 Cuts at: 7504 7730 7882 9991 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 1021 974 763 624 546 355 226 166 152 AseI AT'TA_AT Cuts at: 160 1032 8749 160 Size: 872 7717 1606 Fragments arranged by size: 7717 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10195 AvaI C'yCGr_G Cuts at: 1870 1943 3792 6184 7060 7520 1870 Size: 73 1849 2392 876 460 4545 Fragments arranged by size: 4545 2392 1849 876 460 73 BamHI G'GATC_C Cuts at: 3516 6435 7525 7892 3516 Size: 2919 1090 367 5819 Fragments arranged by size: 5819 2919 1090 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5670 7516 8971 618 Size: 325 217 627 3883 1846 1455 1842 Fragments arranged by size: 3883 1846 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2077 2724 3761 5313 7514 1391 Size: 686 647 1037 1552 2201 4072 Fragments arranged by size: 4072 2201 1552 1037 686 647 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2514 2833 3510 4163 4993 7397 928 Size: 971 615 319 677 653 830 2404 3726 Fragments arranged by size: 3726 2404 971 830 677 653 615 319 BbvCI CC'TCA_GC Cuts at: 7352 7352 Size: 10195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8386 8420 1903 Size: 34 6449 34 3678 Fragments arranged by size: 6449 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2138 2923 3949 4162 6035 8087 9297 2138 Size: 785 1026 213 1873 2052 1210 3036 Fragments arranged by size: 3036 2052 1873 1210 1026 785 213 BclI T'GATC_A Cuts at: 1548 1548 Size: 10195 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3096 8806 136 Size: 107 122 71 631 605 1424 5710 1525 Fragments arranged by size: 5710 1525 1424 631 605 122 107 71 BglII A'GATC_T Cuts at: 5937 10190 5937 Size: 4253 5942 BlpI GC'TnA_GC Cuts at: 2718 3749 5307 5957 7612 2718 Size: 1031 1558 650 1655 5301 Fragments arranged by size: 5301 1655 1558 1031 650 Bme1580I G_kGCm'C Cuts at: 1790 2951 6462 8256 9412 1790 Size: 1161 3511 1794 1156 2573 Fragments arranged by size: 3511 2573 1794 1161 1156 BmgBI CAC'GTC Cuts at: 1318 2124 2451 1318 Size: 806 327 9062 Fragments arranged by size: 9062 806 327 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6139 7926 8884 Size: 416 53 83 399 24 4722 1787 958 Cuts at: 8884 442 Size: 1753 Fragments arranged by size: 4722 1787 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10195 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3451 5161 6421 3451 Size: 1710 1260 7225 Fragments arranged by size: 7225 1710 1260 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 3745 3935 4112 4159 4481 4631 4664 Size: 1094 1412 190 177 47 322 150 33 Cuts at: 4664 4983 5156 5483 5570 6509 8855 1239 Size: 319 173 327 87 939 2346 2579 Fragments arranged by size: 2579 2346 1412 1094 939 327 322 319 190 177 173 150 87 47 33 Bpu10I CC'TnA_GC Cuts at: 4691 7352 4691 Size: 2661 7534 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2501 3644 5741 5816 6695 7217 8320 9185 9483 Size: 1143 2097 75 879 522 1103 865 298 Cuts at: 9483 2501 Size: 3213 Fragments arranged by size: 3213 2097 1143 1103 879 865 522 298 75 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4868 4910 5843 6557 8858 882 Size: 617 3369 42 933 714 2301 2219 Fragments arranged by size: 3369 2301 2219 933 714 617 42 BsaAI yAC'GTr Cuts at: 493 1204 6319 7986 493 Size: 711 5115 1667 2702 Fragments arranged by size: 5115 2702 1667 711 BsaBI GATnn'nnATC Cuts at: 5936 6255 6758 6935 10189 5936 Size: 319 503 177 3254 5942 Fragments arranged by size: 5942 3254 503 319 177 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7028 7238 8002 8384 Size: 53 83 186 936 5495 210 764 382 Cuts at: 8384 9019 275 Size: 635 1451 Fragments arranged by size: 5495 1451 936 764 635 382 210 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4764 5313 6804 8628 9026 9300 9447 9858 Size: 702 549 1491 1824 398 274 147 411 Cuts at: 9858 4062 Size: 4399 Fragments arranged by size: 4399 1824 1491 702 549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2372 2402 3921 3951 4637 Size: 30 165 30 969 30 1519 30 686 Cuts at: 4637 4667 5645 5675 6180 6210 6569 6599 6836 Size: 30 978 30 505 30 359 30 237 Cuts at: 6836 6866 1178 Size: 30 4507 Fragments arranged by size: 4507 1519 978 969 686 505 359 237 165 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2641 2977 5669 1186 Size: 1455 336 2692 5712 Fragments arranged by size: 5712 2692 1455 336 BseYI C'CCAG_C Cuts at: 2079 2391 3094 3235 3856 6749 6977 7288 9398 Size: 312 703 141 621 2893 228 311 2110 Cuts at: 9398 9894 2079 Size: 496 2380 Fragments arranged by size: 2893 2380 2110 703 621 496 312 311 228 141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2375 2719 5596 1163 Size: 573 639 344 2877 5762 Fragments arranged by size: 5762 2877 639 573 344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4253 7645 8406 9434 1540 Size: 275 186 2252 3392 761 1028 2301 Fragments arranged by size: 3392 2301 2252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2724 2951 3761 3827 5313 5496 6462 7070 8256 Size: 227 810 66 1486 183 966 608 1186 Cuts at: 8256 8341 9412 2724 Size: 85 1071 3507 Fragments arranged by size: 3507 1486 1186 1071 966 810 608 227 183 85 66 BsmI GAATG_Cn' Cuts at: 3991 4565 6208 6499 7706 7799 3991 Size: 574 1643 291 1207 93 6387 Fragments arranged by size: 6387 1643 1207 574 291 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2114 4309 5185 5831 2114 Size: 2195 876 646 6478 Fragments arranged by size: 6478 2195 876 646 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2724 2951 3761 3827 5313 5496 Size: 399 287 647 227 810 66 1486 183 Cuts at: 5496 6462 7070 7514 8256 8341 9412 1391 Size: 966 608 444 742 85 1071 2174 Fragments arranged by size: 2174 1486 1071 966 810 742 647 608 444 399 287 227 183 85 66 BspEI T'CCGG_A Cuts at: 5313 9858 5313 Size: 4545 5650 BspHI T'CATG_A Cuts at: 3981 6000 7191 8084 9814 3981 Size: 2019 1191 893 1730 4362 Fragments arranged by size: 4362 2019 1730 1191 893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4369 4484 5631 7550 844 Size: 3525 115 1147 1919 3489 Fragments arranged by size: 3525 3489 1919 1147 115 BsrBI CCG'CTC Cuts at: 1394 1823 2035 8082 1394 Size: 429 212 6047 3507 Fragments arranged by size: 6047 3507 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 2640 4053 4196 5502 8690 8864 65 Size: 1183 1392 1413 143 1306 3188 174 1396 Fragments arranged by size: 3188 1413 1396 1392 1306 1183 174 143 BsrFI r'CCGG_y Cuts at: 1700 1810 5823 8839 9026 1700 Size: 110 4013 3016 187 2869 Fragments arranged by size: 4013 3016 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5661 5676 96 Size: 5565 15 4615 Fragments arranged by size: 5565 4615 15 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10195 BssSI C'ACGA_G Cuts at: 5417 8255 9267 5417 Size: 2838 1012 6345 Fragments arranged by size: 6345 2838 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5452 5610 794 Size: 4658 158 5379 Fragments arranged by size: 5379 4658 158 BstBI TT'CG_AA Cuts at: 8999 8999 Size: 10195 BstEII G'GTnAC_C Cuts at: 1184 3145 4847 1184 Size: 1961 1702 6532 Fragments arranged by size: 6532 1961 1702 BstXI CCAn_nnnn'nTGG Cuts at: 3223 3235 4984 5515 7492 3223 Size: 12 1749 531 1977 5926 Fragments arranged by size: 5926 1977 1749 531 12 BstYI r'GATC_y Cuts at: 1972 2646 2848 2920 3516 5937 6435 6528 6732 Size: 674 202 72 596 2421 498 93 204 Cuts at: 6732 6759 7467 7525 7892 8277 8294 9746 9832 Size: 27 708 58 367 385 17 1452 86 Cuts at: 9832 9844 10190 1972 Size: 12 346 1977 Fragments arranged by size: 2421 1977 1452 708 674 596 498 385 367 346 204 202 93 86 72 58 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2805 5301 6011 6684 7077 2805 Size: 2496 710 673 393 5923 Fragments arranged by size: 5923 2496 710 673 393 BtgI C'CryG_G Cuts at: 513 1065 2004 3219 4779 6094 6513 7485 513 Size: 552 939 1215 1560 1315 419 972 3223 Fragments arranged by size: 3223 1560 1315 1215 972 939 552 419 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2115 2797 487 Size: 683 56 889 682 7885 Fragments arranged by size: 7885 889 683 682 56 BtsI GCAGTG_nn' Cuts at: 3772 5109 5780 7711 8505 8525 3772 Size: 1337 671 1931 794 20 5442 Fragments arranged by size: 5442 1931 1337 794 671 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10195 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3381 3416 529 Size: 35 2817 35 7308 Fragments arranged by size: 7308 2817 35 35 DraI TTT'AAA Cuts at: 4549 7499 4549 Size: 2950 7245 DraIII CAC_nnn'GTG Cuts at: 2044 3205 3727 4813 2044 Size: 1161 522 1086 7426 Fragments arranged by size: 7426 1161 1086 522 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3653 4019 6617 9202 809 Size: 507 2337 366 2598 2585 1802 Fragments arranged by size: 2598 2585 2337 1802 507 366 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2325 3225 Size: 54 1425 50 275 18 216 279 900 Cuts at: 3225 4974 6049 6516 7221 7437 7642 8531 10160 Size: 1749 1075 467 705 216 205 889 1629 Cuts at: 10160 8 Size: 43 Fragments arranged by size: 1749 1629 1425 1075 900 889 705 467 279 275 216 216 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7642 1537 Size: 275 5830 4090 Fragments arranged by size: 5830 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2729 3977 5883 6109 6657 7097 8125 1353 Size: 1376 1248 1906 226 548 440 1028 3423 Fragments arranged by size: 3423 1906 1376 1248 1028 548 440 226 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5933 8782 9156 9302 387 Size: 1064 138 4344 2849 374 146 1280 Fragments arranged by size: 4344 2849 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2722 3759 5311 2722 Size: 1037 1552 7606 Fragments arranged by size: 7606 1552 1037 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2447 2456 2852 3745 3887 3935 Size: 393 701 114 9 396 893 142 48 Cuts at: 3935 4112 4159 4175 4235 4481 4619 4631 4664 Size: 177 47 16 60 246 138 12 33 Cuts at: 4664 4983 5156 5426 5483 5570 6509 6681 7184 Size: 319 173 270 57 87 939 172 503 Cuts at: 7184 7473 8258 8855 9642 9910 1239 Size: 289 785 597 787 268 1524 Fragments arranged by size: 1524 939 893 787 785 701 597 503 396 393 319 289 270 268 246 177 173 172 142 138 114 87 60 57 48 47 33 16 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 4376 7076 4376 Size: 2700 7495 EcoO109I rG'GnC_Cy Cuts at: 2388 2967 4639 4936 5273 6715 2388 Size: 579 1672 297 337 1442 5868 Fragments arranged by size: 5868 1672 1442 579 337 297 EcoRI G'AATT_C Cuts at: 6958 7504 7882 6958 Size: 546 378 9271 Fragments arranged by size: 9271 546 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2857 3058 4180 6670 2857 Size: 201 1122 2490 6382 Fragments arranged by size: 6382 2490 1122 201 FspI TGC'GCA Cuts at: 6350 8701 6350 Size: 2351 7844 HaeII r_GCGC'y Cuts at: 1678 7301 9342 1678 Size: 5623 2041 2531 Fragments arranged by size: 5623 2531 2041 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2356 2372 2388 2404 3505 Size: 32 135 32 784 16 16 16 1101 Cuts at: 3505 3537 3788 3820 4336 4368 7466 7498 8836 Size: 32 251 32 516 32 3098 32 1338 Cuts at: 8836 8868 8910 8942 1373 Size: 32 42 32 2626 Fragments arranged by size: 3098 2626 1338 1101 784 516 251 135 42 32 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 7539 7785 669 Size: 988 281 5601 246 3079 Fragments arranged by size: 5601 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 5231 748 Size: 4483 5712 HpaI GTT'AAC Cuts at: 7785 7785 Size: 10195 KpnI G_GTAC'C Cuts at: 5674 7520 5674 Size: 1846 8349 MfeI C'AATT_G Cuts at: 7794 7794 Size: 10195 MluI A'CGCG_T Cuts at: 10071 10071 Size: 10195 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4129 5745 6019 6336 7137 7305 7393 9309 Size: 2824 1616 274 317 801 168 88 1916 Cuts at: 9309 9493 1305 Size: 184 2007 Fragments arranged by size: 2824 2007 1916 1616 801 317 274 184 168 88 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 2327 3227 4976 7439 Size: 54 1425 343 216 279 900 1749 2463 Cuts at: 7439 10 Size: 2766 Fragments arranged by size: 2766 2463 1749 1425 900 343 279 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2562 3155 3221 3356 3818 3983 4116 Size: 648 1396 593 66 135 462 165 133 Cuts at: 4116 4778 4982 8153 8512 8671 518 Size: 662 204 3171 359 159 2042 Fragments arranged by size: 3171 2042 1396 662 648 593 462 359 204 165 159 135 133 66 MspA1I CmG'CkG Cuts at: 1606 2070 3068 4747 5077 5134 5458 6096 6136 Size: 464 998 1679 330 57 324 638 40 Cuts at: 6136 7148 7611 8288 9436 9681 1606 Size: 1012 463 677 1148 245 2120 Fragments arranged by size: 2120 1679 1148 1012 998 677 638 464 463 330 324 245 57 40 NcoI C'CATG_G Cuts at: 513 1065 2004 4779 513 Size: 552 939 2775 5929 Fragments arranged by size: 5929 2775 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10195 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10195 NotI GC'GGCC_GC Cuts at: 7642 7642 Size: 10195 NspI r_CATG'y Cuts at: 1132 1229 3511 4630 4821 5455 6466 7220 7553 Size: 97 2282 1119 191 634 1011 754 333 Cuts at: 7553 9098 1132 Size: 1545 2229 Fragments arranged by size: 2282 2229 1545 1119 1011 754 634 333 191 97 PasI CC'CwG_GG Cuts at: 1348 7319 1348 Size: 5971 4224 PciI A'CATG_T Cuts at: 6462 9094 6462 Size: 2632 7563 PflMI CCAn_nnn'nTGG Cuts at: 1108 4642 1108 Size: 3534 6661 PfoI T'CCnGG_A Cuts at: 2642 3951 4641 5547 7175 2642 Size: 1309 690 906 1628 5662 Fragments arranged by size: 5662 1628 1309 906 690 PmeI GTTT'AAAC Cuts at: 7499 7499 Size: 10195 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6161 6193 8264 8296 9777 9809 6161 Size: 32 2071 32 1481 32 6547 Fragments arranged by size: 6547 2071 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2388 4639 4936 5273 6715 2388 Size: 2251 297 337 1442 5868 Fragments arranged by size: 5868 2251 1442 337 297 PshAI GACnn'nnGTC Cuts at: 1613 3544 6931 1613 Size: 1931 3387 4877 Fragments arranged by size: 4877 3387 1931 PsiI TTA'TAA Cuts at: 6296 7765 6296 Size: 1469 8726 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3266 3298 3266 Size: 32 10163 PstI C_TGCA'G Cuts at: 830 2094 2254 5133 5491 6971 7153 7547 830 Size: 1264 160 2879 358 1480 182 394 3478 Fragments arranged by size: 3478 2879 1480 1264 394 358 182 160 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10195 PvuII CAG'CTG Cuts at: 3068 4747 5077 5134 5458 6136 7148 3068 Size: 1679 330 57 324 678 1012 6115 Fragments arranged by size: 6115 1679 1012 678 330 324 57 SacI G_AGCT'C Cuts at: 2724 3761 5313 2724 Size: 1037 1552 7606 Fragments arranged by size: 7606 1552 1037 SacII CC_GC'GG Cuts at: 6097 6097 Size: 10195 SalI G'TCGA_C Cuts at: 1936 7537 1936 Size: 5601 4594 SapI GCTCTTCn'nnn_ Cuts at: 2729 2729 Size: 10195 SbfI CC_TGCA'GG Cuts at: 7547 7547 Size: 10195 ScaI AGT'ACT Cuts at: 4655 8443 10155 4655 Size: 3788 1712 4695 Fragments arranged by size: 4695 3788 1712 SexAI A'CCwGG_T Cuts at: 3201 4635 5532 7491 3201 Size: 1434 897 1959 5905 Fragments arranged by size: 5905 1959 1434 897 SfcI C'TryA_G Cuts at: 826 1045 2090 2250 3893 5129 5487 6967 7149 Size: 219 1045 160 1643 1236 358 1480 182 Cuts at: 7149 7543 8678 9359 9550 826 Size: 394 1135 681 191 1471 Fragments arranged by size: 1643 1480 1471 1236 1135 1045 681 394 358 219 191 182 160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10195 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10195 SmaI CCC'GGG Cuts at: 1872 3794 6186 7062 7522 1872 Size: 1922 2392 876 460 4545 Fragments arranged by size: 4545 2392 1922 876 460 SmlI C'TyrA_G Cuts at: 820 1017 1943 2480 2924 3659 5756 5831 6710 Size: 197 926 537 444 735 2097 75 879 Cuts at: 6710 7232 8299 9200 9462 820 Size: 522 1067 901 262 1553 Fragments arranged by size: 2097 1553 1067 926 901 879 735 537 522 444 262 197 75 SnaBI TAC'GTA Cuts at: 493 7986 493 Size: 7493 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10195 SphI G_CATG'C Cuts at: 1132 7220 7553 1132 Size: 6088 333 3774 Fragments arranged by size: 6088 3774 333 SspI AAT'ATT Cuts at: 5 52 8119 5 Size: 47 8067 2081 Fragments arranged by size: 8067 2081 47 StuI AGG'CCT Cuts at: 1517 3138 5544 6650 1517 Size: 1621 2406 1106 5062 Fragments arranged by size: 5062 2406 1621 1106 StyI C'CwwG_G Cuts at: 513 1065 2004 2062 2415 3114 4125 4779 7634 Size: 552 939 58 353 699 1011 654 2855 Cuts at: 7634 513 Size: 3074 Fragments arranged by size: 3074 2855 1011 939 699 654 552 353 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2678 3133 3155 3534 4239 4835 5132 Size: 1255 1098 455 22 379 705 596 297 Cuts at: 5132 5511 7036 8222 8239 8392 8577 9022 325 Size: 379 1525 1186 17 153 185 445 1498 Fragments arranged by size: 1525 1498 1255 1186 1098 705 596 455 445 379 379 297 185 153 22 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3315 3444 4510 Size: 274 80 33 51 1443 1338 129 1066 Cuts at: 4510 4653 5448 5661 5676 8441 10153 96 Size: 143 795 213 15 2765 1712 138 Fragments arranged by size: 2765 1712 1443 1338 1066 795 274 213 143 138 129 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3921 3953 4938 4970 5759 5791 6180 6212 6466 Size: 32 985 32 789 32 389 32 254 Cuts at: 6466 6498 10155 10187 3921 Size: 32 3657 32 3929 Fragments arranged by size: 3929 3657 985 789 389 254 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10195 XbaI T'CTAG_A Cuts at: 7531 7531 Size: 10195 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3157 3814 6943 7291 7376 3157 Size: 657 3129 348 85 5976 Fragments arranged by size: 5976 3129 657 348 85 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10195 XmaI C'CCGG_G Cuts at: 1870 3792 6184 7060 7520 1870 Size: 1922 2392 876 460 4545 Fragments arranged by size: 4545 2392 1922 876 460 XmnI GAAnn'nnTTC Cuts at: 3077 6650 8324 3077 Size: 3573 1674 4948 Fragments arranged by size: 4948 3573 1674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8003 276 Size: 53 83 186 936 6469 2468 Fragments arranged by size: 6469 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SanDI SfiI SfoI SrfI SwaI