Restriction map for
pFN21AA0188
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbeI BcgI BfaI
BfrI BglII BlnI BsiEI BsiWI
Bsp1407I BsrBI BsrGI Bst1107I BstBI
BstZ17I CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FseI FspI HpaI KasI KpnI
KspI MaeI MfeI MluI MunI
NarI NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 1261 TCCGGA T^CCGGA
AflIII 539 ACATGT A^C[GA][CT]GT
ApaI 2248 GGGCCC GGGCC^C
Asp700I 2564 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1215 GGATCC G^GATCC
BanI 1987 GGCACC G^G[CT][GA]CC
BbrPI 1971 CACGTG CAC^GTG
BciVI 2592 GGATAC GTATCC(6/5)
BclI 282 TGATCA T^GATCA
BglI 2241 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 277 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1045 GAGACC GGTCTC(1/5)
BseAI 1261 TCCGGA T^CCGGA
BsmBI 430 GAGACG CGTCTC(1/5)
BspDI 1498 ATCGAT AT^CGAT
BspEI 1261 TCCGGA T^CCGGA
BspHI 1622 TCATGA T^CATGA
BspLU11I 539 ACATGT A^CATGT
BspMI 2207 ACCTGC ACCTGC(4/8)
BsrDI 1573 GCAATG GCAATG(2/0)
BsrFI 22 GCCGGC [GA]^CCGG[CT]
BssHII 1812 GCGCGC G^CGCGC
BssSI 1513 CTCGTG CACGAG(-5/-1)
BstAPI 1270 GCAAAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 707 GGTCACC G^GT[ACGT]ACC
BstPI 707 GGTCACC G^GT[ACGT]ACC
Cfr10I 22 GCCGGC [GA]^CCGG[CT]
ClaI 1498 ATCGAT AT^CGAT
DraI 2323 TTTAAA TTT^AAA
DrdI 94 GACATCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2085 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 707 GGTCACC G^GT[ACGT]ACC
EcoT22I 1442 ATGCAT ATGCA^T
FbaI 282 TGATCA T^GATCA
HaeII 1062 AGCGCC [GA]GCGC^[CT]
HindIII 1908 AAGCTT A^AGCTT
MamI 277 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1261 TCCGGA T^CCGGA
NaeI 22 GCCGGC GCC^GGC
NcoI 572 CCATGG C^CATGG
NdeI 229 CATATG CA^TATG
NgoMIV 22 GCCGGC G^CCGGC
NsiI 1442 ATGCAT ATGCA^T
PmaCI 1971 CACGTG CAC^GTG
PmlI 1971 CACGTG CAC^GTG
RcaI 1622 TCATGA T^CATGA
SalI 2536 GTCGAC G^TCGAC
SfcI 449 CTGTAG C^T[GA][CT]AG
XmnI 2564 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1189 GTCTAC GT^[AC][GT]AC
2536 GTCGAC
AccII 1813 CGCG CG^CG
2198 CGCG
AcsI 899 AAATTT [GA]^AATT[CT]
2152 AAATTT
AcyI 432 GACGCC G[GA]^CG[CT]C
1186 GGCGTC
AocI 637 CCTGAGG CC^T[ACGT]AGG
1049 CCTTAGG
ApoI 899 AAATTT [GA]^AATT[CT]
2152 AAATTT
AspI 432 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1183 GACGGCGTC
AvaI 179 CCCGAG C^[CT]CG[GA]G
1332 CTCGGG
BcnI 1295 CCCGG CC^[GC]GG
1425 CCGGG
BlpI 1761 GCTCAGC GC^T[ACGT]AGC
2116 GCTAAGC
Bpu1102I 1761 GCTCAGC GC^T[ACGT]AGC
2116 GCTAAGC
BsaAI 7 TACGTG [CT]AC^GT[GA]
1971 CACGTG
BsaHI 432 GACGCC G[GA]^CG[CT]C
1186 GGCGTC
BsgI 2209 CTGCAC GTGCAG(16/14)
2287 CTGCAC
BsmI 349 GAATGC GAATGC(1/-1)
1444 GCATTC
BsoBI 179 CCCGAG C^[CT]CG[GA]G
1332 CTCGGG
BstUI 1813 CGCG CG^CG
2198 CGCG
BstYI 1215 GGATCC [GA]^GATC[CT]
1254 AGATCC
Bsu36I 637 CCTGAGG CC^T[ACGT]AGG
1049 CCTTAGG
CelII 1761 GCTCAGC GC^T[ACGT]AGC
2116 GCTAAGC
DsaI 572 CCATGG C^C[GA][CT]GG
779 CCACGG
Eco81I 637 CCTGAGG CC^T[ACGT]AGG
1049 CCTTAGG
Hin1I 432 GACGCC G[GA]^CG[CT]C
1186 GGCGTC
MflI 1215 GGATCC [GA]^GATC[CT]
1254 AGATCC
MseI 1904 TTAA T^TAA
2324 TTAA
MvnI 1813 CGCG CG^CG
2198 CGCG
NciI 1295 CCCGG CC^[GC]GG
1425 CCGGG
PflFI 432 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1183 GACGGCGTC
PpuMI 167 GGGTCCT [GA]G^G[AT]CC[CT]
1376 GGGACCT
SfaNI 1441 GATGC GCATC(5/9)
2111 GCATC
SspI 1535 AATATT AAT^ATT
2451 AATATT
StuI 387 AGGCCT AGG^CCT
1580 AGGCCT
Tru9I 1904 TTAA T^TAA
2324 TTAA
Tth111I 432 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1183 GACGGCGTC
XhoII 1215 GGATCC [GA]^GATC[CT]
1254 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanII BbsI BbvCI
BbvI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI