(Circular) (Six-base) MAPSORT of: pFN21AA0188.seq Check: 2183 from: 1 to: 7375 pFN21AA0188 7375 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5185 278 Size: 53 83 186 936 3649 2468 Fragments arranged by size: 3649 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3195 4542 4718 1937 Size: 1258 1347 176 4594 Fragments arranged by size: 4594 1347 1258 176 Acc65I G'GTAC_C Cuts at: 4696 4696 Size: 7375 AclI AA'CG_TT Cuts at: 1563 5502 5875 1563 Size: 3939 373 3063 Fragments arranged by size: 3939 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 2931 3246 4563 4588 5438 6822 7090 Size: 893 406 315 1317 25 850 1384 268 Cuts at: 7090 1632 Size: 1917 Fragments arranged by size: 1917 1384 1317 893 850 406 315 268 25 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7178 AflIII A'CryG_T Cuts at: 1203 2544 6274 7251 1203 Size: 1341 3730 977 1327 Fragments arranged by size: 3730 1341 1327 977 AgeI A'CCGG_T Cuts at: 6206 6206 Size: 7375 AhdI GACnn_n'nnGTC Cuts at: 6104 6104 Size: 7375 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7375 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4259 1184 Size: 3075 4300 AlwNI CAG_nnn'CTG Cuts at: 5118 6690 5118 Size: 1572 5803 ApaI G_GGCC'C Cuts at: 4257 4257 Size: 7375 ApaLI G'TGCA_C Cuts at: 5432 6588 5432 Size: 1156 6219 ApoI r'AATT_y Cuts at: 1493 2904 4157 4684 4910 5062 7171 1493 Size: 1411 1253 527 226 152 2109 1697 Fragments arranged by size: 2109 1697 1411 1253 527 226 152 AseI AT'TA_AT Cuts at: 160 1032 5929 160 Size: 872 4897 1606 Fragments arranged by size: 4897 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7375 AvaI C'yCGr_G Cuts at: 1870 1943 2184 3337 4700 1870 Size: 73 241 1153 1363 4545 Fragments arranged by size: 4545 1363 1153 241 73 BamHI G'GATC_C Cuts at: 3220 4705 5072 3220 Size: 1485 367 5523 Fragments arranged by size: 5523 1485 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3992 4696 6151 618 Size: 325 217 627 2205 704 1455 1842 Fragments arranged by size: 2205 1842 1455 704 627 325 217 BanII G_rGCy'C Cuts at: 1391 2058 2375 2567 3096 3301 4257 4694 1391 Size: 667 317 192 529 205 956 437 4072 Fragments arranged by size: 4072 956 667 529 437 317 205 192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2847 3048 3887 928 Size: 971 948 201 839 4416 Fragments arranged by size: 4416 971 948 839 201 BbvCI CC'TCA_GC Cuts at: 3377 3419 4669 3377 Size: 42 1250 6083 Fragments arranged by size: 6083 1250 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5566 5600 1903 Size: 34 3629 34 3678 Fragments arranged by size: 3678 3629 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4591 5267 6477 4591 Size: 676 1210 5489 Fragments arranged by size: 5489 1210 676 BclI T'GATC_A Cuts at: 1548 2287 1548 Size: 739 6636 BfrBI ATG'CAT Cuts at: 3449 3449 Size: 7375 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4252 5986 136 Size: 107 122 71 631 605 2580 1734 1525 Fragments arranged by size: 2580 1734 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7370 7370 Size: 7375 BlpI GC'TnA_GC Cuts at: 3767 4122 4792 3767 Size: 355 670 6350 Fragments arranged by size: 6350 670 355 Bme1580I G_kGCm'C Cuts at: 1790 2992 3995 4238 4257 5436 6592 1790 Size: 1202 1003 243 19 1179 1156 2573 Fragments arranged by size: 2573 1202 1179 1156 1003 243 19 BmgBI CAC'GTC Cuts at: 1318 2152 3943 1318 Size: 834 1791 4750 Fragments arranged by size: 4750 1791 834 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2718 4021 4228 Size: 416 53 83 399 24 1301 1303 207 Cuts at: 4228 5106 6064 442 Size: 878 958 1753 Fragments arranged by size: 1753 1303 1301 958 878 416 399 207 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7375 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2581 3382 2581 Size: 801 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2609 3068 3476 3787 6035 1239 Size: 1370 459 408 311 2248 2579 Fragments arranged by size: 2579 2248 1370 459 408 311 Bpu10I CC'TnA_GC Cuts at: 3377 3419 4669 3377 Size: 42 1250 6083 Fragments arranged by size: 6083 1250 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5500 6365 6663 5500 Size: 865 298 6212 Fragments arranged by size: 6212 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3044 6038 882 Size: 617 1545 2994 2219 Fragments arranged by size: 2994 2219 1545 617 BsaAI yAC'GTr Cuts at: 493 1204 2014 3978 5166 493 Size: 711 810 1964 1188 2702 Fragments arranged by size: 2702 1964 1188 810 711 BsaBI GATnn'nnATC Cuts at: 2286 7369 2286 Size: 5083 2292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2438 3192 5182 5564 Size: 53 83 186 936 905 754 1990 382 Cuts at: 5564 6199 275 Size: 635 1451 Fragments arranged by size: 1990 1451 936 905 754 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2732 3266 3746 5808 6206 6480 6627 7038 2732 Size: 534 480 2062 398 274 147 411 3069 Fragments arranged by size: 3069 2062 534 480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3127 3157 3555 3585 4470 Size: 30 165 30 1724 30 398 30 885 Cuts at: 4470 4500 1178 Size: 30 4053 Fragments arranged by size: 4053 1724 885 398 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2494 3104 3560 3827 1186 Size: 1308 610 456 267 4734 Fragments arranged by size: 4734 1308 610 456 267 BseYI C'CCAG_C Cuts at: 2402 2429 4271 6578 7074 2402 Size: 27 1842 2307 496 2703 Fragments arranged by size: 2703 2307 1842 496 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4199 4277 1163 Size: 573 2463 78 4261 Fragments arranged by size: 4261 2463 573 78 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4825 5586 6614 1540 Size: 275 186 2824 761 1028 2301 Fragments arranged by size: 2824 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2058 2567 3096 3365 5436 5521 6592 2058 Size: 509 529 269 2071 85 1071 2841 Fragments arranged by size: 2841 2071 1071 529 509 269 85 BsmI GAATG_Cn' Cuts at: 2360 3449 4886 4979 2360 Size: 1089 1437 93 4756 Fragments arranged by size: 4756 1437 1089 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2429 2429 Size: 7375 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2375 2567 2992 3096 3301 3365 Size: 399 268 317 192 425 104 205 64 Cuts at: 3365 3995 4238 4257 4694 5436 5521 6592 1391 Size: 630 243 19 437 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 630 437 425 399 317 268 243 205 192 104 85 64 19 BspEI T'CCGG_A Cuts at: 3266 7038 3266 Size: 3772 3603 BspHI T'CATG_A Cuts at: 3627 5264 6994 3627 Size: 1637 1730 4008 Fragments arranged by size: 4008 1730 1637 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4221 4730 844 Size: 3377 509 3489 Fragments arranged by size: 3489 3377 509 BsrBI CCG'CTC Cuts at: 1394 1823 5262 1394 Size: 429 3439 3507 Fragments arranged by size: 3507 3439 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3585 5870 6044 65 Size: 1183 2337 2285 174 1396 Fragments arranged by size: 2337 2285 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 6019 6206 1700 Size: 110 217 3992 187 2869 Fragments arranged by size: 3992 2869 217 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7375 BssHII G'CGCG_C Cuts at: 1925 3817 1925 Size: 1892 5483 BssSI C'ACGA_G Cuts at: 3518 5435 6447 3518 Size: 1917 1012 4446 Fragments arranged by size: 4446 1917 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3281 794 Size: 2487 4888 BstBI TT'CG_AA Cuts at: 6179 6179 Size: 7375 BstEII G'GTnAC_C Cuts at: 1184 2712 1184 Size: 1528 5847 BstXI CCAn_nnnn'nTGG Cuts at: 2123 3520 3943 4189 2123 Size: 1397 423 246 5309 Fragments arranged by size: 5309 1397 423 246 BstYI r'GATC_y Cuts at: 1972 3220 3259 4705 5072 5457 5474 6926 7012 Size: 1248 39 1446 367 385 17 1452 86 Cuts at: 7012 7024 7370 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1446 1248 385 367 346 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2643 3055 2643 Size: 412 6963 BtgI C'CryG_G Cuts at: 513 1065 2577 2784 513 Size: 552 1512 207 5104 Fragments arranged by size: 5104 1512 552 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3379 4102 487 Size: 683 56 2153 723 3760 Fragments arranged by size: 3760 2153 723 683 56 BtsI GCAGTG_nn' Cuts at: 3725 4631 4891 5685 5705 3725 Size: 906 260 794 20 5395 Fragments arranged by size: 5395 906 794 260 20 ClaI AT'CG_AT Cuts at: 1554 3504 1554 Size: 1950 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7340 DraI TTT'AAA Cuts at: 4330 4679 4330 Size: 349 7026 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2105 6382 809 Size: 507 789 4277 1802 Fragments arranged by size: 4277 1802 789 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2313 3774 Size: 54 1425 50 275 18 199 284 1461 Cuts at: 3774 4072 4822 5711 7340 8 Size: 298 750 889 1629 43 Fragments arranged by size: 1629 1461 1425 889 750 298 284 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4822 1537 Size: 275 3010 4090 Fragments arranged by size: 4090 3010 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2459 2501 2610 3861 4286 4519 5305 1353 Size: 1106 42 109 1251 425 233 786 3423 Fragments arranged by size: 3423 1251 1106 786 425 233 109 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2102 4208 5962 6336 6482 387 Size: 1064 138 513 2106 1754 374 146 1280 Fragments arranged by size: 2106 1754 1280 1064 513 374 146 138 EcoICRI GAG'CTC Cuts at: 2056 2565 3094 2056 Size: 509 529 6337 Fragments arranged by size: 6337 529 509 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2525 2609 2931 3068 3246 3476 3787 Size: 393 893 84 322 137 178 230 311 Cuts at: 3787 4563 4588 5438 6035 6822 7090 1239 Size: 776 25 850 597 787 268 1524 Fragments arranged by size: 1524 893 850 787 776 597 393 322 311 268 230 178 137 84 25 EcoNI CCTnn'n_nnAGG Cuts at: 4094 4094 Size: 7375 EcoO109I rG'GnC_Cy Cuts at: 2173 3382 3415 4253 4254 2173 Size: 1209 33 838 1 5294 Fragments arranged by size: 5294 1209 838 33 1 EcoRI G'AATT_C Cuts at: 4684 5062 4684 Size: 378 6997 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4593 4593 Size: 7375 FspI TGC'GCA Cuts at: 5881 5881 Size: 7375 HaeII r_GCGC'y Cuts at: 1678 3071 6522 1678 Size: 1393 3451 2531 Fragments arranged by size: 3451 2531 1393 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2426 2458 2659 2691 3127 Size: 32 135 32 854 32 201 32 436 Cuts at: 3127 3159 6016 6048 6090 6122 1373 Size: 32 2857 32 42 32 2626 Fragments arranged by size: 2857 2626 854 436 201 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2200 4225 4468 4543 4719 4965 Size: 988 281 262 2025 243 75 176 246 Cuts at: 4965 669 Size: 3079 Fragments arranged by size: 3079 2025 988 281 262 246 243 176 75 HindIII A'AGCT_T Cuts at: 748 3913 748 Size: 3165 4210 HpaI GTT'AAC Cuts at: 4965 4965 Size: 7375 KpnI G_GTAC'C Cuts at: 4700 4700 Size: 7375 MfeI C'AATT_G Cuts at: 4974 4974 Size: 7375 MluI A'CGCG_T Cuts at: 7251 7251 Size: 7375 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6489 6673 1305 Size: 5184 184 2007 Fragments arranged by size: 5184 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2315 3776 4074 10 Size: 54 1425 343 483 1461 298 3311 Fragments arranged by size: 3311 1461 1425 483 343 298 54 MslI CAynn'nnrTG Cuts at: 518 1166 2558 3180 3215 4187 5333 5692 5851 Size: 648 1392 622 35 972 1146 359 159 Cuts at: 5851 518 Size: 2042 Fragments arranged by size: 2042 1392 1146 972 648 622 359 159 35 MspA1I CmG'CkG Cuts at: 1606 2362 2494 3824 3984 4412 4673 4791 5468 Size: 756 132 1330 160 428 261 118 677 Cuts at: 5468 6616 6861 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1330 1148 756 677 428 261 245 160 132 118 NaeI GCC'GGC Cuts at: 2029 2029 Size: 7375 NcoI C'CATG_G Cuts at: 513 1065 2577 513 Size: 552 1512 5311 Fragments arranged by size: 5311 1512 552 NdeI CA'TA_TG Cuts at: 387 2235 387 Size: 1848 5527 NgoMIV G'CCGG_C Cuts at: 2027 2027 Size: 7375 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7375 NotI GC'GGCC_GC Cuts at: 4822 4822 Size: 7375 NsiI A_TGCA'T Cuts at: 3451 3451 Size: 7375 NspI r_CATG'y Cuts at: 1132 1229 2548 3284 4499 4733 6278 1132 Size: 97 1319 736 1215 234 1545 2229 Fragments arranged by size: 2229 1545 1319 1215 736 234 97 PasI CC'CwG_GG Cuts at: 1348 4249 1348 Size: 2901 4474 PciI A'CATG_T Cuts at: 2544 6274 2544 Size: 3730 3645 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7375 PfoI T'CCnGG_A Cuts at: 3149 3453 3149 Size: 304 7071 PmeI GTTT'AAAC Cuts at: 4679 4679 Size: 7375 PmlI CAC'GTG Cuts at: 3978 3978 Size: 7375 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2531 2563 5444 5476 6957 6989 2531 Size: 32 2881 32 1481 32 2917 Fragments arranged by size: 2917 2881 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2173 3382 2173 Size: 1209 6166 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7375 PsiI TTA'TAA Cuts at: 3546 4945 3546 Size: 1399 5976 PspOMI G'GGCC_C Cuts at: 4253 4253 Size: 7375 PstI C_TGCA'G Cuts at: 830 4727 830 Size: 3897 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7375 PvuII CAG'CTG Cuts at: 2362 2494 3824 4412 2362 Size: 132 1330 588 5325 Fragments arranged by size: 5325 1330 588 132 SacI G_AGCT'C Cuts at: 2058 2567 3096 2058 Size: 509 529 6337 Fragments arranged by size: 6337 529 509 SalI G'TCGA_C Cuts at: 1936 4541 4717 1936 Size: 2605 176 4594 Fragments arranged by size: 4594 2605 176 SbfI CC_TGCA'GG Cuts at: 4727 4727 Size: 7375 ScaI AGT'ACT Cuts at: 5623 7335 5623 Size: 1712 5663 SfcI C'TryA_G Cuts at: 826 1045 2454 4723 5858 6539 6730 826 Size: 219 1409 2269 1135 681 191 1471 Fragments arranged by size: 2269 1471 1409 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7375 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7375 SmaI CCC'GGG Cuts at: 1872 4702 1872 Size: 2830 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5479 6380 6642 820 Size: 197 926 3536 901 262 1553 Fragments arranged by size: 3536 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5166 493 Size: 4673 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7375 SphI G_CATG'C Cuts at: 1132 4733 1132 Size: 3601 3774 SspI AAT'ATT Cuts at: 5 52 3542 4458 5299 5 Size: 47 3490 916 841 2081 Fragments arranged by size: 3490 2081 916 841 47 StuI AGG'CCT Cuts at: 1517 2394 3587 1517 Size: 877 1193 5305 Fragments arranged by size: 5305 1193 877 StyI C'CwwG_G Cuts at: 513 1065 2577 3970 4090 4814 513 Size: 552 1512 1393 120 724 3074 Fragments arranged by size: 3074 1512 1393 724 552 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2731 5402 5419 5572 5757 6202 325 Size: 1255 1151 2671 17 153 185 445 1498 Fragments arranged by size: 2671 1498 1255 1151 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5621 7333 96 Size: 274 80 33 51 1443 3644 1712 138 Fragments arranged by size: 3644 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2693 2710 2725 2742 3555 3587 7335 7367 2693 Size: 17 15 17 813 32 3748 32 2701 Fragments arranged by size: 3748 2701 813 32 32 17 17 15 Tth111I GACn'n_nGTC Cuts at: 1316 2440 3191 1316 Size: 1124 751 5500 Fragments arranged by size: 5500 1124 751 XbaI T'CTAG_A Cuts at: 4711 4711 Size: 7375 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2156 2584 2817 2011 Size: 145 428 233 6569 Fragments arranged by size: 6569 428 233 145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7375 XmaI C'CCGG_G Cuts at: 1870 4700 1870 Size: 2830 4545 XmnI GAAnn'nnTTC Cuts at: 4573 5504 4573 Size: 931 6444 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5183 276 Size: 53 83 186 936 3649 2468 Fragments arranged by size: 3649 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI