Restriction map for
pFN21AA0188
(Linear) (Six-base) MAPSORT of: KIAA0188.seq Check: 1834 from: 1 to: 2670
KIAA0188.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 1190 2537 2670
Size: 1190 1347 133
Fragments arranged by size:
1347 1190 133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 926 1241 2558 2583 2670
Size: 520 406 315 1317 25 87
Fragments arranged by size:
1317 520 406 315 87 25
AflIII A'CryG_T
Cuts at: 0 539 2670
Size: 539 2131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2254 2670
Size: 2254 416
ApaI G_GGCC'C
Cuts at: 0 2252 2670
Size: 2252 418
ApoI r'AATT_y
Cuts at: 0 899 2152 2670
Size: 899 1253 518
Fragments arranged by size:
1253 899 518
AvaI C'yCGr_G
Cuts at: 0 179 1332 2670
Size: 179 1153 1338
Fragments arranged by size:
1338 1153 179
BamHI G'GATC_C
Cuts at: 0 1215 2670
Size: 1215 1455
BanI G'GyrC_C
Cuts at: 0 1987 2670
Size: 1987 683
BanII G_rGCy'C
Cuts at: 0 53 370 562 1091 1296 2252 2670
Size: 53 317 192 529 205 956 418
Fragments arranged by size:
956 529 418 317 205 192 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 842 1043 1882 2670
Size: 842 201 839 788
Fragments arranged by size:
842 839 788 201
BbvCI CC'TCA_GC
Cuts at: 0 1372 1414 2664 2670
Size: 1372 42 1250 6
Fragments arranged by size:
1372 1250 42 6
BciVI GTATCCnnnnn_n'
Cuts at: 0 2586 2670
Size: 2586 84
BclI T'GATC_A
Cuts at: 0 282 2670
Size: 282 2388
BfrBI ATG'CAT
Cuts at: 0 1444 2670
Size: 1444 1226
BglI GCCn_nnn'nGGC
Cuts at: 0 2247 2670
Size: 2247 423
BlpI GC'TnA_GC
Cuts at: 0 1762 2117 2670
Size: 1762 355 553
Fragments arranged by size:
1762 553 355
Bme1580I G_kGCm'C
Cuts at: 0 987 1990 2233 2252 2670
Size: 987 1003 243 19 418
Fragments arranged by size:
1003 987 418 243 19
BmgBI CAC'GTC
Cuts at: 0 147 1938 2670
Size: 147 1791 732
Fragments arranged by size:
1791 732 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 713 2016 2223 2670
Size: 713 1303 207 447
Fragments arranged by size:
1303 713 447 207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 576 1377 2670
Size: 576 801 1293
Fragments arranged by size:
1293 801 576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1063 1471 1782 2670
Size: 604 459 408 311 888
Fragments arranged by size:
888 604 459 408 311
Bpu10I CC'TnA_GC
Cuts at: 0 1372 1414 2664 2670
Size: 1372 42 1250 6
Fragments arranged by size:
1372 1250 42 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 1039 2670
Size: 1039 1631
BsaAI yAC'GTr
Cuts at: 0 9 1973 2670
Size: 9 1964 697
Fragments arranged by size:
1964 697 9
BsaBI GATnn'nnATC
Cuts at: 0 281 2670
Size: 281 2389
BsaHI Gr'CG_yC
Cuts at: 0 433 1187 2670
Size: 433 754 1483
Fragments arranged by size:
1483 754 433
BsaWI w'CCGG_w
Cuts at: 0 727 1261 1741 2670
Size: 727 534 480 929
Fragments arranged by size:
929 727 534 480
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1122 1152 1550 1580 2465 2495 2670
Size: 1122 30 398 30 885 30 175
Fragments arranged by size:
1122 885 398 175 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 489 1099 1555 1822 2670
Size: 489 610 456 267 848
Fragments arranged by size:
848 610 489 456 267
BseYI C'CCAG_C
Cuts at: 0 397 424 2266 2670
Size: 397 27 1842 404
Fragments arranged by size:
1842 404 397 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2194 2272 2670
Size: 2194 78 398
Fragments arranged by size:
2194 398 78
BsiHKAI G_wGCw'C
Cuts at: 0 53 562 1091 1360 2670
Size: 53 509 529 269 1310
Fragments arranged by size:
1310 529 509 269 53
BsmI GAATG_Cn'
Cuts at: 0 355 1444 2670
Size: 355 1089 1226
Fragments arranged by size:
1226 1089 355
BsmBI CGTCTCn'nnnn_
Cuts at: 0 424 2670
Size: 424 2246
Bsp1286I G_dGCh'C
Cuts at: 0 53 370 562 987 1091 1296 1360 1990
Size: 53 317 192 425 104 205 64 630
Cuts at: 1990 2233 2252 2670
Size: 243 19 418
Fragments arranged by size:
630 425 418 317 243 205 192 104
64 53 19
BspEI T'CCGG_A
Cuts at: 0 1261 2670
Size: 1261 1409
BspHI T'CATG_A
Cuts at: 0 1622 2670
Size: 1622 1048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2216 2670
Size: 2216 454
BsrDI GCAATG_nn'
Cuts at: 0 1580 2670
Size: 1580 1090
BsrFI r'CCGG_y
Cuts at: 0 22 2670
Size: 22 2648
BssHII G'CGCG_C
Cuts at: 0 1812 2670
Size: 1812 858
BssSI C'ACGA_G
Cuts at: 0 1513 2670
Size: 1513 1157
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1276 2670
Size: 1276 1394
BstEII G'GTnAC_C
Cuts at: 0 707 2670
Size: 707 1963
BstXI CCAn_nnnn'nTGG
Cuts at: 0 118 1515 1938 2184 2670
Size: 118 1397 423 246 486
Fragments arranged by size:
1397 486 423 246 118
BstYI r'GATC_y
Cuts at: 0 1215 1254 2670
Size: 1215 39 1416
Fragments arranged by size:
1416 1215 39
Bsu36I CC'TnA_GG
Cuts at: 0 638 1050 2670
Size: 638 412 1620
Fragments arranged by size:
1620 638 412
BtgI C'CryG_G
Cuts at: 0 572 779 2670
Size: 572 207 1891
Fragments arranged by size:
1891 572 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1374 2097 2670
Size: 1374 723 573
Fragments arranged by size:
1374 723 573
BtsI GCAGTG_nn'
Cuts at: 0 1720 2626 2670
Size: 1720 906 44
Fragments arranged by size:
1720 906 44
ClaI AT'CG_AT
Cuts at: 0 1499 2670
Size: 1499 1171
DraI TTT'AAA
Cuts at: 0 2325 2670
Size: 2325 345
DrdI GACnn_nn'nnGTC
Cuts at: 0 100 2670
Size: 100 2570
EaeI y'GGCC_r
Cuts at: 0 24 308 1769 2067 2670
Size: 24 284 1461 298 603
Fragments arranged by size:
1461 603 298 284 24
EarI CTCTTCn'nnn_
Cuts at: 0 454 496 605 1856 2281 2514 2670
Size: 454 42 109 1251 425 233 156
Fragments arranged by size:
1251 454 425 233 156 109 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 97 2203 2670
Size: 97 2106 467
Fragments arranged by size:
2106 467 97
EcoICRI GAG'CTC
Cuts at: 0 51 560 1089 2670
Size: 51 509 529 1581
Fragments arranged by size:
1581 529 509 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 604 926 1063 1241 1471 1782 2558
Size: 520 84 322 137 178 230 311 776
Cuts at: 2558 2583 2670
Size: 25 87
Fragments arranged by size:
776 520 322 311 230 178 137 87
84 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2089 2670
Size: 2089 581
EcoO109I rG'GnC_Cy
Cuts at: 0 168 1377 1410 2248 2249 2670
Size: 168 1209 33 838 1 421
Fragments arranged by size:
1209 838 421 168 33 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2588 2670
Size: 2588 82
HaeII r_GCGC'y
Cuts at: 0 1066 2670
Size: 1066 1604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 654 686 1122 1154 2670
Size: 421 32 201 32 436 32 1516
Fragments arranged by size:
1516 436 421 201 32 32 32
HincII GTy'rAC
Cuts at: 0 195 2220 2463 2538 2670
Size: 195 2025 243 75 132
Fragments arranged by size:
2025 243 195 132 75
HindIII A'AGCT_T
Cuts at: 0 1908 2670
Size: 1908 762
MscI TGG'CCA
Cuts at: 0 310 1771 2069 2670
Size: 310 1461 298 601
Fragments arranged by size:
1461 601 310 298
MslI CAynn'nnrTG
Cuts at: 0 553 1175 1210 2182 2670
Size: 553 622 35 972 488
Fragments arranged by size:
972 622 553 488 35
MspA1I CmG'CkG
Cuts at: 0 357 489 1819 1979 2407 2670
Size: 357 132 1330 160 428 263
Fragments arranged by size:
1330 428 357 263 160 132
NaeI GCC'GGC
Cuts at: 0 24 2670
Size: 24 2646
NcoI C'CATG_G
Cuts at: 0 572 2670
Size: 572 2098
NdeI CA'TA_TG
Cuts at: 0 230 2670
Size: 230 2440
NgoMIV G'CCGG_C
Cuts at: 0 22 2670
Size: 22 2648
NsiI A_TGCA'T
Cuts at: 0 1446 2670
Size: 1446 1224
NspI r_CATG'y
Cuts at: 0 543 1279 2494 2670
Size: 543 736 1215 176
Fragments arranged by size:
1215 736 543 176
PasI CC'CwG_GG
Cuts at: 0 2244 2670
Size: 2244 426
PciI A'CATG_T
Cuts at: 0 539 2670
Size: 539 2131
PfoI T'CCnGG_A
Cuts at: 0 1144 1448 2670
Size: 1144 304 1222
Fragments arranged by size:
1222 1144 304
PmlI CAC'GTG
Cuts at: 0 1973 2670
Size: 1973 697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 526 558 2670
Size: 526 32 2112
Fragments arranged by size:
2112 526 32
PpuMI rG'GwC_Cy
Cuts at: 0 168 1377 2670
Size: 168 1209 1293
Fragments arranged by size:
1293 1209 168
PsiI TTA'TAA
Cuts at: 0 1541 2670
Size: 1541 1129
PspOMI G'GGCC_C
Cuts at: 0 2248 2670
Size: 2248 422
PvuII CAG'CTG
Cuts at: 0 357 489 1819 2407 2670
Size: 357 132 1330 588 263
Fragments arranged by size:
1330 588 357 263 132
SacI G_AGCT'C
Cuts at: 0 53 562 1091 2670
Size: 53 509 529 1579
Fragments arranged by size:
1579 529 509 53
SalI G'TCGA_C
Cuts at: 0 2536 2670
Size: 2536 134
SfcI C'TryA_G
Cuts at: 0 449 2670
Size: 449 2221
SspI AAT'ATT
Cuts at: 0 1537 2453 2670
Size: 1537 916 217
Fragments arranged by size:
1537 916 217
StuI AGG'CCT
Cuts at: 0 389 1582 2670
Size: 389 1193 1088
Fragments arranged by size:
1193 1088 389
StyI C'CwwG_G
Cuts at: 0 572 1965 2085 2670
Size: 572 1393 120 585
Fragments arranged by size:
1393 585 572 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 726 2670
Size: 726 1944
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 688 705 720 737 1550 1582 2670
Size: 688 17 15 17 813 32 1088
Fragments arranged by size:
1088 813 688 32 17 17 15
Tth111I GACn'n_nGTC
Cuts at: 0 435 1186 2670
Size: 435 751 1484
Fragments arranged by size:
1484 751 435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 151 579 812 2670
Size: 151 428 233 1858
Fragments arranged by size:
1858 428 233 151
XmnI GAAnn'nnTTC
Cuts at: 0 2568 2670
Size: 2568 102
Enzymes that do cut:
AccI AcuI AflIII AlfI ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PfoI PmlI PpiI
PpuMI PsiI PspOMI PvuII SacI SalI SfcI SspI
StuI StyI TaqII TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BglII BmtI BpuEI BsiEI BsiWI
BsrBI BsrGI BstBI BstZ17I CspCI DraIII EagI EcoRI
EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MmeI NarI NheI NotI NruI PacI PflMI
PmeI PshAI PspXI PsrI PstI PvuI RsrII SacII
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
TatI XbaI XhoI XmaI ZraI