(Circular) (Six-base) MAPSORT of: pFN21AA0104.seq Check: 9831 from: 1 to: 5581 pFN21AA0104 5581 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3391 278 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2924 1937 Size: 987 4594 Acc65I G'GTAC_C Cuts at: 2902 2902 Size: 5581 AclI AA'CG_TT Cuts at: 1563 3708 4081 1563 Size: 2145 373 3063 Fragments arranged by size: 3063 2145 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3644 5028 5296 1632 Size: 2012 1384 268 1917 Fragments arranged by size: 2012 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5384 AflIII A'CryG_T Cuts at: 1203 4480 5457 1203 Size: 3277 977 1327 Fragments arranged by size: 3277 1327 977 AgeI A'CCGG_T Cuts at: 2182 4412 2182 Size: 2230 3351 AhdI GACnn_n'nnGTC Cuts at: 2480 4310 2480 Size: 1830 3751 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5581 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2052 1184 Size: 868 4713 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2210 2242 2210 Size: 32 5549 AlwNI CAG_nnn'CTG Cuts at: 2404 3324 4896 2404 Size: 920 1572 3089 Fragments arranged by size: 3089 1572 920 ApaLI G'TGCA_C Cuts at: 3638 4794 3638 Size: 1156 4425 ApoI r'AATT_y Cuts at: 1493 2240 2276 2713 2801 2890 3116 3268 5377 Size: 747 36 437 88 89 226 152 2109 Cuts at: 5377 1493 Size: 1697 Fragments arranged by size: 2109 1697 747 437 226 152 89 88 36 AseI AT'TA_AT Cuts at: 160 1032 4135 160 Size: 872 3103 1606 Fragments arranged by size: 3103 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 AvaI C'yCGr_G Cuts at: 1870 1943 2511 2906 1870 Size: 73 568 395 4545 Fragments arranged by size: 4545 568 395 73 AvrII C'CTAG_G Cuts at: 2047 2047 Size: 5581 BamHI G'GATC_C Cuts at: 2911 3278 2911 Size: 367 5214 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2902 4357 618 Size: 325 217 627 1115 1455 1842 Fragments arranged by size: 1842 1455 1115 627 325 217 BanII G_rGCy'C Cuts at: 1391 2157 2900 1391 Size: 766 743 4072 Fragments arranged by size: 4072 766 743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2141 2175 3772 3806 1903 Size: 34 204 34 1597 34 3678 Fragments arranged by size: 3678 1597 204 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3473 4683 3473 Size: 1210 4371 BclI T'GATC_A Cuts at: 1548 1548 Size: 5581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2128 4192 Size: 107 122 71 631 605 337 119 2064 Cuts at: 4192 136 Size: 1525 Fragments arranged by size: 2064 1525 631 605 337 122 119 107 71 BglII A'GATC_T Cuts at: 5576 5576 Size: 5581 BlpI GC'TnA_GC Cuts at: 2998 2998 Size: 5581 Bme1580I G_kGCm'C Cuts at: 1790 2031 3642 4798 1790 Size: 241 1611 1156 2573 Fragments arranged by size: 2573 1611 1156 241 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5581 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 2151 3312 Size: 416 53 83 399 24 624 110 1161 Cuts at: 3312 4270 442 Size: 958 1753 Fragments arranged by size: 1753 1161 958 624 416 399 110 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5581 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4241 1239 Size: 3002 2579 Bpu10I CC'TnA_GC Cuts at: 2682 2682 Size: 5581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2251 3706 4571 4869 2251 Size: 1455 865 298 2963 Fragments arranged by size: 2963 1455 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4244 882 Size: 617 2745 2219 Fragments arranged by size: 2745 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3372 493 Size: 711 2168 2702 Fragments arranged by size: 2702 2168 711 BsaBI GATnn'nnATC Cuts at: 5575 5575 Size: 5581 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3388 3770 4405 275 Size: 53 83 186 936 1855 382 635 1451 Fragments arranged by size: 1855 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2182 2215 4014 4412 4686 4833 5244 2182 Size: 33 1799 398 274 147 411 2519 Fragments arranged by size: 2519 1799 411 398 274 147 33 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5356 Fragments arranged by size: 5356 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5581 BseYI C'CCAG_C Cuts at: 2700 4784 5280 2700 Size: 2084 496 3001 Fragments arranged by size: 3001 2084 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5008 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3031 3792 4820 1540 Size: 275 186 1030 761 1028 2301 Fragments arranged by size: 2301 1030 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2141 3642 3727 4798 2141 Size: 1501 85 1071 2924 Fragments arranged by size: 2924 1501 1071 85 BsmI GAATG_Cn' Cuts at: 2415 3092 3185 2415 Size: 677 93 4811 Fragments arranged by size: 4811 677 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2141 2157 2900 3642 3727 4798 Size: 399 241 110 16 743 742 85 1071 Cuts at: 4798 1391 Size: 2174 Fragments arranged by size: 2174 1071 743 742 399 241 110 85 16 BspEI T'CCGG_A Cuts at: 5244 5244 Size: 5581 BspHI T'CATG_A Cuts at: 3470 5200 3470 Size: 1730 3851 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2214 2936 844 Size: 1370 722 3489 Fragments arranged by size: 3489 1370 722 BsrBI CCG'CTC Cuts at: 1394 1823 3468 1394 Size: 429 1645 3507 Fragments arranged by size: 3507 1645 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2016 2046 4076 4250 65 Size: 1183 768 30 2030 174 1396 Fragments arranged by size: 2030 1396 1183 768 174 30 BsrFI r'CCGG_y Cuts at: 1700 1810 2089 2182 4225 4412 1700 Size: 110 279 93 2043 187 2869 Fragments arranged by size: 2869 2043 279 187 110 93 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5581 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5581 BssSI C'ACGA_G Cuts at: 3641 4653 3641 Size: 1012 4569 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5581 BstBI TT'CG_AA Cuts at: 4385 4385 Size: 5581 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5581 BstYI r'GATC_y Cuts at: 1972 2266 2911 3278 3663 3680 5132 5218 5230 Size: 294 645 367 385 17 1452 86 12 Cuts at: 5230 5576 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 645 385 367 346 294 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2456 2456 Size: 5581 BtgI C'CryG_G Cuts at: 513 1065 2004 2208 2792 513 Size: 552 939 204 584 3302 Fragments arranged by size: 3302 939 584 552 204 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4842 Fragments arranged by size: 4842 683 56 BtsI GCAGTG_nn' Cuts at: 3097 3891 3911 3097 Size: 794 20 4767 Fragments arranged by size: 4767 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5581 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5546 DraI TTT'AAA Cuts at: 2885 2885 Size: 5581 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4588 809 Size: 507 3272 1802 Fragments arranged by size: 3272 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3028 3917 5546 Size: 54 1425 50 275 18 1198 889 1629 Cuts at: 5546 8 Size: 43 Fragments arranged by size: 1629 1425 1198 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3028 1537 Size: 275 1216 4090 Fragments arranged by size: 4090 1216 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2425 3511 1353 Size: 1072 1086 3423 Fragments arranged by size: 3423 1086 1072 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4168 4542 4688 387 Size: 1064 138 2579 374 146 1280 Fragments arranged by size: 2579 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3644 4241 5028 5296 1239 Size: 393 2012 597 787 268 1524 Fragments arranged by size: 2012 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2120 2120 Size: 5581 EcoRI G'AATT_C Cuts at: 2240 2890 3268 2240 Size: 650 378 4553 Fragments arranged by size: 4553 650 378 FspI TGC'GCA Cuts at: 4087 4087 Size: 5581 HaeII r_GCGC'y Cuts at: 1678 4728 1678 Size: 3050 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2466 2498 4222 4254 4296 Size: 32 135 32 894 32 1724 32 42 Cuts at: 4296 4328 1373 Size: 32 2626 Fragments arranged by size: 2626 1724 894 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2925 3171 669 Size: 988 281 987 246 3079 Fragments arranged by size: 3079 988 987 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5581 HpaI GTT'AAC Cuts at: 3171 3171 Size: 5581 KpnI G_GTAC'C Cuts at: 2906 2906 Size: 5581 MfeI C'AATT_G Cuts at: 3180 3180 Size: 5581 MluI A'CGCG_T Cuts at: 5457 5457 Size: 5581 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2191 2323 4695 4879 1305 Size: 886 132 2372 184 2007 Fragments arranged by size: 2372 2007 886 184 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3759 Fragments arranged by size: 3759 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3539 3898 4057 518 Size: 648 2373 359 159 2042 Fragments arranged by size: 2373 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2024 2369 2997 3674 4822 5067 1606 Size: 418 345 628 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 628 418 345 245 NaeI GCC'GGC Cuts at: 2091 2091 Size: 5581 NcoI C'CATG_G Cuts at: 513 1065 2004 2792 513 Size: 552 939 788 3302 Fragments arranged by size: 3302 939 788 552 NdeI CA'TA_TG Cuts at: 387 2377 387 Size: 1990 3591 NgoMIV G'CCGG_C Cuts at: 2089 2089 Size: 5581 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5581 NotI GC'GGCC_GC Cuts at: 3028 3028 Size: 5581 NspI r_CATG'y Cuts at: 1132 1229 2939 4484 1132 Size: 97 1710 1545 2229 Fragments arranged by size: 2229 1710 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5581 PciI A'CATG_T Cuts at: 4480 4480 Size: 5581 PflMI CCAn_nnn'nTGG Cuts at: 1108 2404 1108 Size: 1296 4285 PfoI T'CCnGG_A Cuts at: 2517 2517 Size: 5581 PmeI GTTT'AAAC Cuts at: 2885 2885 Size: 5581 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2254 2286 3650 3682 5163 5195 2254 Size: 32 1364 32 1481 32 2640 Fragments arranged by size: 2640 1481 1364 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5581 PsiI TTA'TAA Cuts at: 2505 3151 2505 Size: 646 4935 PstI C_TGCA'G Cuts at: 830 2108 2933 830 Size: 1278 825 3478 Fragments arranged by size: 3478 1278 825 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5581 PvuII CAG'CTG Cuts at: 2369 2369 Size: 5581 SalI G'TCGA_C Cuts at: 1936 2923 1936 Size: 987 4594 SbfI CC_TGCA'GG Cuts at: 2108 2933 2108 Size: 825 4756 ScaI AGT'ACT Cuts at: 2319 3829 5541 2319 Size: 1510 1712 2359 Fragments arranged by size: 2359 1712 1510 SfcI C'TryA_G Cuts at: 826 1045 2104 2364 2929 4064 4745 4936 826 Size: 219 1059 260 565 1135 681 191 1471 Fragments arranged by size: 1471 1135 1059 681 565 260 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5581 SmaI CCC'GGG Cuts at: 1872 2908 1872 Size: 1036 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2230 3685 4586 4848 820 Size: 197 926 287 1455 901 262 1553 Fragments arranged by size: 1553 1455 926 901 287 262 197 SnaBI TAC'GTA Cuts at: 493 3372 493 Size: 2879 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5581 SphI G_CATG'C Cuts at: 1132 2939 1132 Size: 1807 3774 SspI AAT'ATT Cuts at: 5 52 2722 3505 5 Size: 47 2670 783 2081 Fragments arranged by size: 2670 2081 783 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5581 StyI C'CwwG_G Cuts at: 513 1065 2004 2047 2792 3020 513 Size: 552 939 43 745 228 3074 Fragments arranged by size: 3074 939 745 552 228 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3608 3625 3778 3963 4408 325 Size: 1255 2028 17 153 185 445 1498 Fragments arranged by size: 2028 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2317 2626 3827 Size: 274 80 33 51 1443 340 309 1201 Cuts at: 3827 5539 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1201 340 309 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5541 5573 5541 Size: 32 5549 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5581 XbaI T'CTAG_A Cuts at: 2917 2917 Size: 5581 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5581 XmaI C'CCGG_G Cuts at: 1870 2906 1870 Size: 1036 4545 XmnI GAAnn'nnTTC Cuts at: 2244 3710 2244 Size: 1466 4115 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3389 276 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI