(Circular) (Six-base) MAPSORT of: pFN21AA0013.seq Check: 8904 from: 1 to: 7774 pFN21AA0013 7774 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3182 3691 3182 Size: 509 7265 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5584 278 Size: 53 83 186 936 4048 2468 Fragments arranged by size: 4048 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4835 5117 1937 Size: 2898 282 4594 Fragments arranged by size: 4594 2898 282 Acc65I G'GTAC_C Cuts at: 5095 5095 Size: 7774 AclI AA'CG_TT Cuts at: 1563 5901 6274 1563 Size: 4338 373 3063 Fragments arranged by size: 4338 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 5837 7221 7489 1632 Size: 968 3237 1384 268 1917 Fragments arranged by size: 3237 1917 1384 968 268 AflII C'TTAA_G Cuts at: 820 1017 2366 4496 820 Size: 197 1349 2130 4098 Fragments arranged by size: 4098 2130 1349 197 AflIII A'CryG_T Cuts at: 1203 4307 4373 6673 7650 1203 Size: 3104 66 2300 977 1327 Fragments arranged by size: 3104 2300 1327 977 66 AgeI A'CCGG_T Cuts at: 3100 6605 3100 Size: 3505 4269 AhdI GACnn_n'nnGTC Cuts at: 6503 6503 Size: 7774 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7774 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7774 AlwNI CAG_nnn'CTG Cuts at: 2020 2047 2276 4391 5517 7089 2020 Size: 27 229 2115 1126 1572 2705 Fragments arranged by size: 2705 2115 1572 1126 229 27 ApaLI G'TGCA_C Cuts at: 5831 6987 5831 Size: 1156 6618 ApoI r'AATT_y Cuts at: 1493 2891 3317 4151 5083 5309 5461 7570 1493 Size: 1398 426 834 932 226 152 2109 1697 Fragments arranged by size: 2109 1697 1398 932 834 426 226 152 AseI AT'TA_AT Cuts at: 160 1032 4539 6328 160 Size: 872 3507 1789 1606 Fragments arranged by size: 3507 1789 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7774 AvaI C'yCGr_G Cuts at: 1870 1943 5099 1870 Size: 73 3156 4545 Fragments arranged by size: 4545 3156 73 AvrII C'CTAG_G Cuts at: 4334 4334 Size: 7774 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3087 3120 4996 5029 3087 Size: 33 1876 33 5832 Fragments arranged by size: 5832 1876 33 33 BamHI G'GATC_C Cuts at: 5104 5471 5104 Size: 367 7407 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5095 6550 618 Size: 325 217 627 3308 1455 1842 Fragments arranged by size: 3308 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2397 5093 1391 Size: 1006 2696 4072 Fragments arranged by size: 4072 2696 1006 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2241 2480 2637 2857 928 Size: 971 342 239 157 220 5845 Fragments arranged by size: 5845 971 342 239 220 157 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2078 2112 5965 5999 1903 Size: 34 141 34 3853 34 3678 Fragments arranged by size: 3853 3678 141 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5666 6876 5666 Size: 1210 6564 BclI T'GATC_A Cuts at: 1548 3183 4429 1548 Size: 1635 1246 4893 Fragments arranged by size: 4893 1635 1246 BfrBI ATG'CAT Cuts at: 2708 2708 Size: 7774 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6385 136 Size: 107 122 71 631 605 4713 1525 Fragments arranged by size: 4713 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3302 4857 7769 3302 Size: 1555 2912 3307 Fragments arranged by size: 3307 2912 1555 BlpI GC'TnA_GC Cuts at: 2982 5191 2982 Size: 2209 5565 Bme1580I G_kGCm'C Cuts at: 1790 5835 6991 1790 Size: 4045 1156 2573 Fragments arranged by size: 4045 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7774 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5505 6463 442 Size: 416 53 83 399 24 4088 958 1753 Fragments arranged by size: 4088 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7774 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4792 4792 Size: 7774 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3513 4141 6434 1239 Size: 2274 628 2293 2579 Fragments arranged by size: 2579 2293 2274 628 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2910 3004 3656 5899 6764 7062 2910 Size: 94 652 2243 865 298 3622 Fragments arranged by size: 3622 2243 865 652 298 94 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2780 4972 6437 882 Size: 617 1281 2192 1465 2219 Fragments arranged by size: 2219 2192 1465 1281 617 BsaAI yAC'GTr Cuts at: 493 1204 5565 493 Size: 711 4361 2702 Fragments arranged by size: 4361 2702 711 BsaBI GATnn'nnATC Cuts at: 7768 7768 Size: 7774 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5581 5963 6598 275 Size: 53 83 186 936 4048 382 635 1451 Fragments arranged by size: 4048 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3100 3555 6207 6605 6879 7026 7437 3100 Size: 455 2652 398 274 147 411 3437 Fragments arranged by size: 3437 2652 455 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2856 2886 4591 4621 1178 Size: 30 165 30 1453 30 1705 30 4331 Fragments arranged by size: 4331 1705 1453 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7774 BseYI C'CCAG_C Cuts at: 2404 2943 6977 7473 2404 Size: 539 4034 496 2705 Fragments arranged by size: 4034 2705 539 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2322 4278 1163 Size: 573 586 1956 4659 Fragments arranged by size: 4659 1956 586 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2098 5224 5985 7013 1540 Size: 275 186 97 3126 761 1028 2301 Fragments arranged by size: 3126 2301 1028 761 275 186 97 BsiHKAI G_wGCw'C Cuts at: 2884 4642 5835 5920 6991 2884 Size: 1758 1193 85 1071 3667 Fragments arranged by size: 3667 1758 1193 1071 85 BsmI GAATG_Cn' Cuts at: 4825 5285 5378 4825 Size: 460 93 7221 Fragments arranged by size: 7221 460 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2397 2884 4642 5093 5835 5920 6991 Size: 399 607 487 1758 451 742 85 1071 Cuts at: 6991 1391 Size: 2174 Fragments arranged by size: 2174 1758 1071 742 607 487 451 399 85 BspEI T'CCGG_A Cuts at: 7437 7437 Size: 7774 BspHI T'CATG_A Cuts at: 4906 5663 7393 4906 Size: 757 1730 5287 Fragments arranged by size: 5287 1730 757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3182 3691 5129 844 Size: 2338 509 1438 3489 Fragments arranged by size: 3489 2338 1438 509 BsrBI CCG'CTC Cuts at: 1394 1823 5661 1394 Size: 429 3838 3507 Fragments arranged by size: 3838 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2990 6269 6443 65 Size: 1183 1742 3279 174 1396 Fragments arranged by size: 3279 1742 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3100 6418 6605 1700 Size: 110 1290 3318 187 2869 Fragments arranged by size: 3318 2869 1290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7774 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7774 BssSI C'ACGA_G Cuts at: 5834 6846 5834 Size: 1012 6762 BstAPI GCAn_nnn'nTGC Cuts at: 794 2047 794 Size: 1253 6521 BstBI TT'CG_AA Cuts at: 3298 6578 3298 Size: 3280 4494 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7774 BstYI r'GATC_y Cuts at: 1972 2273 2555 3302 4283 4857 5104 5471 5856 Size: 301 282 747 981 574 247 367 385 Cuts at: 5856 5873 7325 7411 7423 7769 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 981 747 574 385 367 346 301 282 247 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4973 4973 Size: 7774 BtgI C'CryG_G Cuts at: 513 1065 2083 2121 513 Size: 552 1018 38 6166 Fragments arranged by size: 6166 1018 552 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7035 Fragments arranged by size: 7035 683 56 BtsI GCAGTG_nn' Cuts at: 2096 2162 3186 5290 6084 6104 2096 Size: 66 1024 2104 794 20 3766 Fragments arranged by size: 3766 2104 1024 794 66 20 ClaI AT'CG_AT Cuts at: 1554 2700 3117 1554 Size: 1146 417 6211 Fragments arranged by size: 6211 1146 417 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7739 DraI TTT'AAA Cuts at: 2896 3414 3717 4461 5078 2896 Size: 518 303 744 617 5592 Fragments arranged by size: 5592 744 617 518 303 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6781 809 Size: 507 5465 1802 Fragments arranged by size: 5465 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4870 5221 6110 Size: 54 1425 50 275 18 3040 351 889 Cuts at: 6110 7739 8 Size: 1629 43 Fragments arranged by size: 3040 1629 1425 889 351 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5221 1537 Size: 275 3409 4090 Fragments arranged by size: 4090 3409 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3086 4725 5704 1353 Size: 1733 1639 979 3423 Fragments arranged by size: 3423 1733 1639 979 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6361 6735 6881 387 Size: 1064 138 4772 374 146 1280 Fragments arranged by size: 4772 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2600 3513 4141 5837 6434 7221 7489 Size: 393 968 913 628 1696 597 787 268 Cuts at: 7489 1239 Size: 1524 Fragments arranged by size: 1696 1524 968 913 787 628 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3572 4700 4969 4998 3572 Size: 1128 269 29 6348 Fragments arranged by size: 6348 1128 269 29 EcoRI G'AATT_C Cuts at: 4151 5083 5461 4151 Size: 932 378 6464 Fragments arranged by size: 6464 932 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4903 4903 Size: 7774 FspI TGC'GCA Cuts at: 6280 6280 Size: 7774 HaeII r_GCGC'y Cuts at: 1678 6921 1678 Size: 5243 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2693 2725 3110 3142 4268 Size: 32 135 32 1121 32 385 32 1126 Cuts at: 4268 4300 6415 6447 6489 6521 1373 Size: 32 2115 32 42 32 2626 Fragments arranged by size: 2626 2115 1126 1121 385 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5118 5364 669 Size: 988 281 3180 246 3079 Fragments arranged by size: 3180 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2203 3612 4451 748 Size: 1455 1409 839 4071 Fragments arranged by size: 4071 1455 1409 839 HpaI GTT'AAC Cuts at: 5364 5364 Size: 7774 KpnI G_GTAC'C Cuts at: 5099 5099 Size: 7774 MfeI C'AATT_G Cuts at: 5373 5373 Size: 7774 MluI A'CGCG_T Cuts at: 7650 7650 Size: 7774 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3523 4530 6888 7072 1305 Size: 2218 1007 2358 184 2007 Fragments arranged by size: 2358 2218 2007 1007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4872 10 Size: 54 1425 343 3040 2912 Fragments arranged by size: 3040 2912 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5732 6091 6250 518 Size: 648 4566 359 159 2042 Fragments arranged by size: 4566 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2408 5190 5867 7015 7260 1606 Size: 802 2782 677 1148 245 2120 Fragments arranged by size: 2782 2120 1148 802 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7222 NdeI CA'TA_TG Cuts at: 387 387 Size: 7774 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7774 NotI GC'GGCC_GC Cuts at: 5221 5221 Size: 7774 NsiI A_TGCA'T Cuts at: 2710 2710 Size: 7774 NspI r_CATG'y Cuts at: 1132 1229 4311 4377 5132 6677 1132 Size: 97 3082 66 755 1545 2229 Fragments arranged by size: 3082 2229 1545 755 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7774 PciI A'CATG_T Cuts at: 4307 4373 6673 4307 Size: 66 2300 5408 Fragments arranged by size: 5408 2300 66 PflMI CCAn_nnn'nTGG Cuts at: 1108 2189 1108 Size: 1081 6693 PfoI T'CCnGG_A Cuts at: 2739 2739 Size: 7774 PmeI GTTT'AAAC Cuts at: 5078 5078 Size: 7774 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5843 5875 7356 7388 5843 Size: 32 1481 32 6229 Fragments arranged by size: 6229 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3572 4700 4969 3572 Size: 1128 269 6377 Fragments arranged by size: 6377 1128 269 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7774 PsiI TTA'TAA Cuts at: 4848 5344 4848 Size: 496 7278 PstI C_TGCA'G Cuts at: 830 2683 3193 5126 830 Size: 1853 510 1933 3478 Fragments arranged by size: 3478 1933 1853 510 PvuI CG_AT'CG Cuts at: 2001 2098 2001 Size: 97 7677 PvuII CAG'CTG Cuts at: 2408 2408 Size: 7774 SalI G'TCGA_C Cuts at: 1936 5116 1936 Size: 3180 4594 SbfI CC_TGCA'GG Cuts at: 5126 5126 Size: 7774 ScaI AGT'ACT Cuts at: 6022 7734 6022 Size: 1712 6062 SfcI C'TryA_G Cuts at: 826 1045 2679 3189 3954 4569 4839 5064 5122 Size: 219 1634 510 765 615 270 225 58 Cuts at: 5122 6257 6938 7129 826 Size: 1135 681 191 1471 Fragments arranged by size: 1634 1471 1135 765 681 615 510 270 225 219 191 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7774 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7774 SmaI CCC'GGG Cuts at: 1872 5101 1872 Size: 3229 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 2925 3019 3635 4496 5878 Size: 197 926 423 559 94 616 861 1382 Cuts at: 5878 6779 7041 820 Size: 901 262 1553 Fragments arranged by size: 1553 1382 926 901 861 616 559 423 262 197 94 SnaBI TAC'GTA Cuts at: 493 5565 493 Size: 5072 2702 SpeI A'CTAG_T Cuts at: 152 3538 152 Size: 3386 4388 SphI G_CATG'C Cuts at: 1132 5132 1132 Size: 4000 3774 SspI AAT'ATT Cuts at: 5 52 2142 2598 4741 5698 5 Size: 47 2090 456 2143 957 2081 Fragments arranged by size: 2143 2090 2081 957 456 47 StuI AGG'CCT Cuts at: 1517 4339 1517 Size: 2822 4952 StyI C'CwwG_G Cuts at: 513 1065 3200 4334 4887 5213 513 Size: 552 2135 1134 553 326 3074 Fragments arranged by size: 3074 2135 1134 553 552 326 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4821 5801 5818 5971 6156 6601 325 Size: 1255 3241 980 17 153 185 445 1498 Fragments arranged by size: 3241 1498 1255 980 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2686 3160 4304 Size: 274 80 33 51 1443 709 474 1144 Cuts at: 4304 6020 7732 96 Size: 1716 1712 138 Fragments arranged by size: 1716 1712 1443 1144 709 474 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7734 7766 7734 Size: 32 7742 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7774 XbaI T'CTAG_A Cuts at: 5110 5110 Size: 7774 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3787 3787 Size: 7774 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7774 XmaI C'CCGG_G Cuts at: 1870 5099 1870 Size: 3229 4545 XmnI GAAnn'nnTTC Cuts at: 2911 3294 4423 4540 4784 5903 2911 Size: 383 1129 117 244 1119 4782 Fragments arranged by size: 4782 1129 1119 383 244 117 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5582 276 Size: 53 83 186 936 4048 2468 Fragments arranged by size: 4048 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI