Restriction map for
pFN21AA0013
(Linear) (Six-base) MAPSORT of: KIAA0013.seq Check: 624 from: 1 to: 3069
KIAA0013.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1177 1686 3069
Size: 1177 509 1383
Fragments arranged by size:
1383 1177 509
AccI GT'mk_AC
Cuts at: 0 2830 3069
Size: 2830 239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 3069
Size: 595 2474
AflII C'TTAA_G
Cuts at: 0 361 2491 3069
Size: 361 2130 578
Fragments arranged by size:
2130 578 361
AflIII A'CryG_T
Cuts at: 0 2302 2368 3069
Size: 2302 66 701
Fragments arranged by size:
2302 701 66
AgeI A'CCGG_T
Cuts at: 0 1095 3069
Size: 1095 1974
AlwNI CAG_nnn'CTG
Cuts at: 0 15 42 271 2386 3069
Size: 15 27 229 2115 683
Fragments arranged by size:
2115 683 229 27 15
ApoI r'AATT_y
Cuts at: 0 886 1312 2146 3069
Size: 886 426 834 923
Fragments arranged by size:
923 886 834 426
AseI AT'TA_AT
Cuts at: 0 2534 3069
Size: 2534 535
AvrII C'CTAG_G
Cuts at: 0 2329 3069
Size: 2329 740
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1082 1115 2991 3024 3069
Size: 1082 33 1876 33 45
Fragments arranged by size:
1876 1082 45 33 33
BanII G_rGCy'C
Cuts at: 0 392 3069
Size: 392 2677
BbsI GAAGACnn'nnnn_
Cuts at: 0 236 475 632 852 3069
Size: 236 239 157 220 2217
Fragments arranged by size:
2217 239 236 220 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 107 3069
Size: 73 34 2962
Fragments arranged by size:
2962 73 34
BclI T'GATC_A
Cuts at: 0 1178 2424 3069
Size: 1178 1246 645
Fragments arranged by size:
1246 1178 645
BfrBI ATG'CAT
Cuts at: 0 703 3069
Size: 703 2366
BglII A'GATC_T
Cuts at: 0 1297 2852 3069
Size: 1297 1555 217
Fragments arranged by size:
1555 1297 217
BlpI GC'TnA_GC
Cuts at: 0 977 3069
Size: 977 2092
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2787 3069
Size: 2787 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1508 2136 3069
Size: 1508 628 933
Fragments arranged by size:
1508 933 628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 999 1651 3069
Size: 905 94 652 1418
Fragments arranged by size:
1418 905 652 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 775 2967 3069
Size: 775 2192 102
Fragments arranged by size:
2192 775 102
BsaWI w'CCGG_w
Cuts at: 0 1095 1550 3069
Size: 1095 455 1519
Fragments arranged by size:
1519 1095 455
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 851 881 2586 2616 3069
Size: 851 30 1705 30 453
Fragments arranged by size:
1705 851 453 30 30
BseYI C'CCAG_C
Cuts at: 0 399 938 3069
Size: 399 539 2131
Fragments arranged by size:
2131 539 399
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 2273 3069
Size: 317 1956 796
Fragments arranged by size:
1956 796 317
BsiEI CG_ry'CG
Cuts at: 0 93 3069
Size: 93 2976
BsiHKAI G_wGCw'C
Cuts at: 0 879 2637 3069
Size: 879 1758 432
Fragments arranged by size:
1758 879 432
BsmI GAATG_Cn'
Cuts at: 0 2820 3069
Size: 2820 249
Bsp1286I G_dGCh'C
Cuts at: 0 392 879 2637 3069
Size: 392 487 1758 432
Fragments arranged by size:
1758 487 432 392
BspHI T'CATG_A
Cuts at: 0 2901 3069
Size: 2901 168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1177 1686 3069
Size: 1177 509 1383
Fragments arranged by size:
1383 1177 509
BsrDI GCAATG_nn'
Cuts at: 0 985 3069
Size: 985 2084
BsrFI r'CCGG_y
Cuts at: 0 1095 3069
Size: 1095 1974
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 3069
Size: 42 3027
BstBI TT'CG_AA
Cuts at: 0 1293 3069
Size: 1293 1776
BstYI r'GATC_y
Cuts at: 0 268 550 1297 2278 2852 3069
Size: 268 282 747 981 574 217
Fragments arranged by size:
981 747 574 282 268 217
Bsu36I CC'TnA_GG
Cuts at: 0 2968 3069
Size: 2968 101
BtgI C'CryG_G
Cuts at: 0 78 116 3069
Size: 78 38 2953
Fragments arranged by size:
2953 78 38
BtsI GCAGTG_nn'
Cuts at: 0 91 157 1181 3069
Size: 91 66 1024 1888
Fragments arranged by size:
1888 1024 91 66
ClaI AT'CG_AT
Cuts at: 0 695 1112 3069
Size: 695 417 1957
Fragments arranged by size:
1957 695 417
DraI TTT'AAA
Cuts at: 0 891 1409 1712 2456 3069
Size: 891 518 303 744 613
Fragments arranged by size:
891 744 613 518 303
EaeI y'GGCC_r
Cuts at: 0 2865 3069
Size: 2865 204
EarI CTCTTCn'nnn_
Cuts at: 0 1081 2720 3069
Size: 1081 1639 349
Fragments arranged by size:
1639 1081 349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1508 2136 3069
Size: 595 913 628 933
Fragments arranged by size:
933 913 628 595
EcoO109I rG'GnC_Cy
Cuts at: 0 1567 2695 2964 2993 3069
Size: 1567 1128 269 29 76
Fragments arranged by size:
1567 1128 269 76 29
EcoRI G'AATT_C
Cuts at: 0 2146 3069
Size: 2146 923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2898 3069
Size: 2898 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 688 720 1105 1137 2263 2295 3069
Size: 688 32 385 32 1126 32 774
Fragments arranged by size:
1126 774 688 385 32 32 32
HindIII A'AGCT_T
Cuts at: 0 198 1607 2446 3069
Size: 198 1409 839 623
Fragments arranged by size:
1409 839 623 198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1518 2525 3069
Size: 1518 1007 544
Fragments arranged by size:
1518 1007 544
MscI TGG'CCA
Cuts at: 0 2867 3069
Size: 2867 202
MspA1I CmG'CkG
Cuts at: 0 403 3069
Size: 403 2666
NsiI A_TGCA'T
Cuts at: 0 705 3069
Size: 705 2364
NspI r_CATG'y
Cuts at: 0 2306 2372 3069
Size: 2306 66 697
Fragments arranged by size:
2306 697 66
PciI A'CATG_T
Cuts at: 0 2302 2368 3069
Size: 2302 66 701
Fragments arranged by size:
2302 701 66
PflMI CCAn_nnn'nTGG
Cuts at: 0 184 3069
Size: 184 2885
PfoI T'CCnGG_A
Cuts at: 0 734 3069
Size: 734 2335
PpuMI rG'GwC_Cy
Cuts at: 0 1567 2695 2964 3069
Size: 1567 1128 269 105
Fragments arranged by size:
1567 1128 269 105
PsiI TTA'TAA
Cuts at: 0 2843 3069
Size: 2843 226
PstI C_TGCA'G
Cuts at: 0 678 1188 3069
Size: 678 510 1881
Fragments arranged by size:
1881 678 510
PvuI CG_AT'CG
Cuts at: 0 93 3069
Size: 93 2976
PvuII CAG'CTG
Cuts at: 0 403 3069
Size: 403 2666
SfcI C'TryA_G
Cuts at: 0 674 1184 1949 2564 2834 3059 3069
Size: 674 510 765 615 270 225 10
Fragments arranged by size:
765 674 615 510 270 225 10
SmlI C'TyrA_G
Cuts at: 0 361 920 1014 1630 2491 3069
Size: 361 559 94 616 861 578
Fragments arranged by size:
861 616 578 559 361 94
SpeI A'CTAG_T
Cuts at: 0 1533 3069
Size: 1533 1536
SspI AAT'ATT
Cuts at: 0 137 593 2736 3069
Size: 137 456 2143 333
Fragments arranged by size:
2143 456 333 137
StuI AGG'CCT
Cuts at: 0 2334 3069
Size: 2334 735
StyI C'CwwG_G
Cuts at: 0 1195 2329 2882 3069
Size: 1195 1134 553 187
Fragments arranged by size:
1195 1134 553 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2816 3069
Size: 2816 253
TatI w'GTAC_w
Cuts at: 0 681 1155 2299 3069
Size: 681 474 1144 770
Fragments arranged by size:
1144 770 681 474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1782 3069
Size: 1782 1287
XmnI GAAnn'nnTTC
Cuts at: 0 906 1289 2418 2535 2779 3069
Size: 906 383 1129 117 244 290
Fragments arranged by size:
1129 906 383 290 244 117
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AgeI AlwNI ApoI
AseI AvrII BaeI BanII BbsI BcgI BclI BfrBI
BglII BlpI BplI BpmI BpuEI BsaI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstBI BstYI Bsu36I BtgI BtsI
ClaI DraI EaeI EarI Eco57MI EcoO109I EcoRI FalI
Hin4I HindIII MmeI MscI MspA1I NsiI NspI PciI
PflMI PfoI PpuMI PsiI PstI PvuI PvuII SfcI
SmlI SpeI SspI StuI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AhdI AleI AlfI AloI
ApaI ApaLI AscI AsiSI AvaI BamHI BanI BbeI
BbvCI BciVI BglI Bme1580I BmgBI BmrI BmtI Bpu10I
BsaAI BsaBI BsaHI BseRI BsiWI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI