(Circular) (Six-base) MAPSORT of: pFN21AA0011.seq Check: 5253 from: 1 to: 7438 pFN21AA0011 7438 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3619 3619 Size: 7438 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 5248 278 Size: 53 83 186 936 2404 1308 2468 Fragments arranged by size: 2468 2404 1308 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2073 3541 4781 1937 Size: 136 1468 1240 4594 Fragments arranged by size: 4594 1468 1240 136 Acc65I G'GTAC_C Cuts at: 4759 4759 Size: 7438 AclI AA'CG_TT Cuts at: 1563 5565 5938 1563 Size: 4002 373 3063 Fragments arranged by size: 4002 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2106 2244 2486 2573 2622 2787 2838 3123 Size: 474 138 242 87 49 165 51 285 Cuts at: 3123 4323 5501 6885 7153 1632 Size: 1200 1178 1384 268 1917 Fragments arranged by size: 1917 1384 1200 1178 474 285 268 242 165 138 87 51 49 AfeI AGC'GCT Cuts at: 3161 3968 3161 Size: 807 6631 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 6337 7314 1203 Size: 5134 977 1327 Fragments arranged by size: 5134 1327 977 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 6167 6167 Size: 7438 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7438 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3410 3442 3410 Size: 32 7406 AlwNI CAG_nnn'CTG Cuts at: 2596 5181 6753 2596 Size: 2585 1572 3281 Fragments arranged by size: 3281 2585 1572 ApaI G_GGCC'C Cuts at: 2651 2792 3376 2651 Size: 141 584 6713 Fragments arranged by size: 6713 584 141 ApaLI G'TGCA_C Cuts at: 5495 6651 5495 Size: 1156 6282 ApoI r'AATT_y Cuts at: 1493 3920 4747 4973 5125 7234 1493 Size: 2427 827 226 152 2109 1697 Fragments arranged by size: 2427 2109 1697 827 226 152 AseI AT'TA_AT Cuts at: 160 1032 5992 160 Size: 872 4960 1606 Fragments arranged by size: 4960 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 4763 1870 Size: 73 2820 4545 Fragments arranged by size: 4545 2820 73 AvrII C'CTAG_G Cuts at: 2285 2285 Size: 7438 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2567 2600 2567 Size: 33 7405 BamHI G'GATC_C Cuts at: 4142 4768 5135 4142 Size: 626 367 6445 Fragments arranged by size: 6445 626 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3110 3210 3458 4121 4759 Size: 325 217 627 1323 100 248 663 638 Cuts at: 4759 6214 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1323 663 638 627 325 248 217 100 BanII G_rGCy'C Cuts at: 1391 2610 2651 2792 2804 3202 3376 4191 4654 Size: 1219 41 141 12 398 174 815 463 Cuts at: 4654 4757 1391 Size: 103 4072 Fragments arranged by size: 4072 1219 815 463 398 174 141 103 41 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2748 928 Size: 971 849 5618 Fragments arranged by size: 5618 971 849 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3219 3253 5629 5663 1903 Size: 34 1282 34 2376 34 3678 Fragments arranged by size: 3678 2376 1282 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 3777 5330 6540 2002 Size: 1775 1553 1210 2900 Fragments arranged by size: 2900 1775 1553 1210 BclI T'GATC_A Cuts at: 1548 2197 1548 Size: 649 6789 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2573 3563 3700 Size: 107 122 71 631 605 901 990 137 Cuts at: 3700 6049 136 Size: 2349 1525 Fragments arranged by size: 2349 1525 990 901 631 605 137 122 107 71 BglII A'GATC_T Cuts at: 2251 4276 7433 2251 Size: 2025 3157 2256 Fragments arranged by size: 3157 2256 2025 BlpI GC'TnA_GC Cuts at: 3567 4855 3567 Size: 1288 6150 Bme1580I G_kGCm'C Cuts at: 1790 2651 2792 2829 3215 3376 5499 6655 1790 Size: 861 141 37 386 161 2123 1156 2573 Fragments arranged by size: 2573 2123 1156 861 386 161 141 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7438 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2986 3209 3429 Size: 416 53 83 399 24 1569 223 220 Cuts at: 3429 4631 5169 6127 442 Size: 1202 538 958 1753 Fragments arranged by size: 1753 1569 1202 958 538 416 399 223 220 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2229 2229 Size: 7438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2199 2218 2738 3244 3305 4526 6098 1239 Size: 960 19 520 506 61 1221 1572 2579 Fragments arranged by size: 2579 1572 1221 960 520 506 61 19 Bpu10I CC'TnA_GC Cuts at: 2814 3677 4023 2814 Size: 863 346 6229 Fragments arranged by size: 6229 863 346 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3053 3407 3994 5563 6428 6726 3053 Size: 354 587 1569 865 298 3765 Fragments arranged by size: 3765 1569 865 587 354 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2281 2390 2525 3500 6101 882 Size: 617 782 109 135 975 2601 2219 Fragments arranged by size: 2601 2219 975 782 617 135 109 BsaAI yAC'GTr Cuts at: 493 1204 5229 493 Size: 711 4025 2702 Fragments arranged by size: 4025 2702 711 BsaBI GATnn'nnATC Cuts at: 7432 7432 Size: 7438 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 5245 5627 6262 Size: 53 83 186 936 2404 1308 382 635 Cuts at: 6262 275 Size: 1451 Fragments arranged by size: 2404 1451 1308 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3550 3909 5871 6269 6543 6690 7101 3550 Size: 359 1962 398 274 147 411 3887 Fragments arranged by size: 3887 1962 411 398 359 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2703 2733 3050 3080 4529 Size: 30 165 30 1300 30 317 30 1449 Cuts at: 4529 4559 1178 Size: 30 4057 Fragments arranged by size: 4057 1449 1300 317 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2218 3074 3465 4101 1186 Size: 1032 856 391 636 4523 Fragments arranged by size: 4523 1032 856 636 391 BseYI C'CCAG_C Cuts at: 3162 6641 7137 3162 Size: 3479 496 3463 Fragments arranged by size: 3479 3463 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2157 1163 Size: 573 421 6444 Fragments arranged by size: 6444 573 421 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4888 5649 6677 1540 Size: 275 186 2887 761 1028 2301 Fragments arranged by size: 2887 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2610 2731 2804 4191 5499 5584 6655 2610 Size: 121 73 1387 1308 85 1071 3393 Fragments arranged by size: 3393 1387 1308 1071 121 85 73 BsmI GAATG_Cn' Cuts at: 4474 4949 5042 4474 Size: 475 93 6870 Fragments arranged by size: 6870 475 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2610 2651 2731 2792 2804 2829 3202 Size: 399 820 41 80 61 12 25 373 Cuts at: 3202 3215 3376 4191 4654 4757 5499 5584 6655 Size: 13 161 815 463 103 742 85 1071 Cuts at: 6655 1391 Size: 2174 Fragments arranged by size: 2174 1071 820 815 742 463 399 373 161 103 85 80 61 41 25 13 12 BspEI T'CCGG_A Cuts at: 3550 7101 3550 Size: 3551 3887 BspHI T'CATG_A Cuts at: 5327 7057 5327 Size: 1730 5708 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2021 3057 3619 4793 844 Size: 1177 1036 562 1174 3489 Fragments arranged by size: 3489 1177 1174 1036 562 BsrBI CCG'CTC Cuts at: 1394 1823 3041 3193 5325 1394 Size: 429 1218 152 2132 3507 Fragments arranged by size: 3507 2132 1218 429 152 BsrDI GCAATG_nn' Cuts at: 65 1248 4191 5933 6107 65 Size: 1183 2943 1742 174 1396 Fragments arranged by size: 2943 1742 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 6082 6269 1700 Size: 110 235 4037 187 2869 Fragments arranged by size: 4037 2869 235 187 110 BsrGI T'GTAC_A Cuts at: 96 3082 96 Size: 2986 4452 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7438 BssSI C'ACGA_G Cuts at: 2841 5498 6510 2841 Size: 2657 1012 3769 Fragments arranged by size: 3769 2657 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7438 BstBI TT'CG_AA Cuts at: 4588 6242 4588 Size: 1654 5784 BstEII G'GTnAC_C Cuts at: 1184 4079 1184 Size: 2895 4543 BstXI CCAn_nnnn'nTGG Cuts at: 3162 3162 Size: 7438 BstYI r'GATC_y Cuts at: 1972 2251 2682 2846 4142 4276 4768 5135 5520 Size: 279 431 164 1296 134 492 367 385 Cuts at: 5520 5537 6989 7075 7087 7433 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1296 492 431 385 367 346 279 164 134 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2520 2643 2520 Size: 123 7315 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2732 4603 513 Size: 552 939 48 680 1871 3348 Fragments arranged by size: 3348 1871 939 680 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4703 487 Size: 683 56 3477 3222 Fragments arranged by size: 3477 3222 683 56 BtsI GCAGTG_nn' Cuts at: 2872 3591 4104 4954 5748 5768 2872 Size: 719 513 850 794 20 4542 Fragments arranged by size: 4542 850 794 719 513 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7438 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7403 DraI TTT'AAA Cuts at: 4742 4742 Size: 7438 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6445 809 Size: 507 5129 1802 Fragments arranged by size: 5129 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2302 2489 4885 Size: 54 1425 50 275 18 472 187 2396 Cuts at: 4885 5774 7403 8 Size: 889 1629 43 Fragments arranged by size: 2396 1629 1425 889 472 275 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4885 1537 Size: 275 3073 4090 Fragments arranged by size: 4090 3073 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2125 2303 2598 2709 5368 1353 Size: 772 178 295 111 2659 3423 Fragments arranged by size: 3423 2659 772 295 178 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6025 6399 6545 387 Size: 1064 138 4436 374 146 1280 Fragments arranged by size: 4436 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2608 2802 4189 2608 Size: 194 1387 5857 Fragments arranged by size: 5857 1387 194 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2199 2218 2244 2486 2573 2622 Size: 393 474 93 19 26 242 87 49 Cuts at: 2622 2738 2787 2838 3123 3244 3305 4323 4526 Size: 116 49 51 285 121 61 1018 203 Cuts at: 4526 5501 6098 6885 7153 1239 Size: 975 597 787 268 1524 Fragments arranged by size: 1524 1018 975 787 597 474 393 285 268 242 203 121 116 93 87 61 51 49 49 26 19 EcoNI CCTnn'n_nnAGG Cuts at: 2039 3053 4116 2039 Size: 1014 1063 5361 Fragments arranged by size: 5361 1063 1014 EcoO109I rG'GnC_Cy Cuts at: 2289 2333 2343 2514 2647 2648 2788 3308 3331 Size: 44 10 171 133 1 140 520 23 Cuts at: 3331 3339 3372 3418 3556 3702 3928 4336 2289 Size: 8 33 46 138 146 226 408 5391 Fragments arranged by size: 5391 520 408 226 171 146 140 138 133 46 44 33 23 10 8 1 EcoRI G'AATT_C Cuts at: 4747 5125 4747 Size: 378 7060 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2594 3034 3988 4117 2594 Size: 440 954 129 5915 Fragments arranged by size: 5915 954 440 129 FseI GG_CCGG'CC Cuts at: 2049 2049 Size: 7438 FspI TGC'GCA Cuts at: 4454 5944 4454 Size: 1490 5948 HaeII r_GCGC'y Cuts at: 1678 3163 3970 6585 1678 Size: 1485 807 2615 2531 Fragments arranged by size: 2615 2531 1485 807 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2789 2821 3050 3082 4273 Size: 32 135 32 1217 32 229 32 1191 Cuts at: 4273 4305 6079 6111 6153 6185 1373 Size: 32 1774 32 42 32 2626 Fragments arranged by size: 2626 1774 1217 1191 229 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4378 4782 5028 669 Size: 988 281 2440 404 246 3079 Fragments arranged by size: 3079 2440 988 404 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7438 HpaI GTT'AAC Cuts at: 5028 5028 Size: 7438 KpnI G_GTAC'C Cuts at: 4763 4763 Size: 7438 MfeI C'AATT_G Cuts at: 5037 5037 Size: 7438 MluI A'CGCG_T Cuts at: 7314 7314 Size: 7438 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2424 4170 4696 4733 6552 6736 1305 Size: 1119 1746 526 37 1819 184 2007 Fragments arranged by size: 2007 1819 1746 1119 526 184 37 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5616 Fragments arranged by size: 5616 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2066 2417 4659 5396 5755 5914 518 Size: 648 900 351 2242 737 359 159 2042 Fragments arranged by size: 2242 2042 900 737 648 359 351 159 MspA1I CmG'CkG Cuts at: 1606 3781 4504 4854 5531 6679 6924 1606 Size: 2175 723 350 677 1148 245 2120 Fragments arranged by size: 2175 2120 1148 723 677 350 245 NaeI GCC'GGC Cuts at: 2047 2047 Size: 7438 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5947 Fragments arranged by size: 5947 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7438 NgoMIV G'CCGG_C Cuts at: 2045 2045 Size: 7438 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7438 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NspI r_CATG'y Cuts at: 1132 1229 4459 4796 6341 1132 Size: 97 3230 337 1545 2229 Fragments arranged by size: 3230 2229 1545 337 97 PasI CC'CwG_GG Cuts at: 1348 2035 1348 Size: 687 6751 PciI A'CATG_T Cuts at: 6337 6337 Size: 7438 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7438 PfoI T'CCnGG_A Cuts at: 2080 2080 Size: 7438 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5507 5539 7020 7052 5507 Size: 32 1481 32 5893 Fragments arranged by size: 5893 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3308 3339 3418 3928 4336 3308 Size: 31 79 510 408 6410 Fragments arranged by size: 6410 510 408 79 31 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7438 PsiI TTA'TAA Cuts at: 3732 5008 3732 Size: 1276 6162 PspOMI G'GGCC_C Cuts at: 2647 2788 3372 2647 Size: 141 584 6713 Fragments arranged by size: 6713 584 141 PstI C_TGCA'G Cuts at: 830 3630 3780 4171 4503 4790 830 Size: 2800 150 391 332 287 3478 Fragments arranged by size: 3478 2800 391 332 287 150 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 PvuII CAG'CTG Cuts at: 4504 4504 Size: 7438 SacI G_AGCT'C Cuts at: 2610 2804 4191 2610 Size: 194 1387 5857 Fragments arranged by size: 5857 1387 194 SalI G'TCGA_C Cuts at: 1936 4780 1936 Size: 2844 4594 SanDI GG'GwC_CC Cuts at: 4336 4336 Size: 7438 SapI GCTCTTCn'nnn_ Cuts at: 2598 2598 Size: 7438 SbfI CC_TGCA'GG Cuts at: 4790 4790 Size: 7438 ScaI AGT'ACT Cuts at: 3528 5686 7398 3528 Size: 2158 1712 3568 Fragments arranged by size: 3568 2158 1712 SfcI C'TryA_G Cuts at: 826 1045 2820 3626 3648 3776 4167 4245 4499 Size: 219 1775 806 22 128 391 78 254 Cuts at: 4499 4786 5921 6602 6793 826 Size: 287 1135 681 191 1471 Fragments arranged by size: 1775 1471 1135 806 681 391 287 254 219 191 128 78 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 4765 1872 Size: 2893 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3032 3422 3973 5542 6443 6705 Size: 197 926 1089 390 551 1569 901 262 Cuts at: 6705 820 Size: 1553 Fragments arranged by size: 1569 1553 1089 926 901 551 390 262 197 SnaBI TAC'GTA Cuts at: 493 5229 493 Size: 4736 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 4459 4796 1132 Size: 3327 337 3774 Fragments arranged by size: 3774 3327 337 SspI AAT'ATT Cuts at: 5 52 5362 5 Size: 47 5310 2081 Fragments arranged by size: 5310 2081 47 StuI AGG'CCT Cuts at: 1517 4097 1517 Size: 2580 4858 StyI C'CwwG_G Cuts at: 513 1065 2004 2285 2445 2652 2946 3334 4084 Size: 552 939 281 160 207 294 388 750 Cuts at: 4084 4350 4877 513 Size: 266 527 3074 Fragments arranged by size: 3074 939 750 552 527 388 294 281 266 207 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3300 5465 5482 5635 5820 6265 325 Size: 1255 1720 2165 17 153 185 445 1498 Fragments arranged by size: 2165 1720 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3082 3526 3977 Size: 274 80 33 51 1443 1105 444 451 Cuts at: 3977 5684 7396 96 Size: 1707 1712 138 Fragments arranged by size: 1712 1707 1443 1105 451 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 2299 2331 2703 2735 3136 3168 3382 Size: 32 191 32 372 32 401 32 214 Cuts at: 3382 3414 7398 7430 2076 Size: 32 3984 32 2084 Fragments arranged by size: 3984 2084 401 372 214 191 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7438 XbaI T'CTAG_A Cuts at: 4774 4774 Size: 7438 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3383 4601 3383 Size: 1218 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7438 XmaI C'CCGG_G Cuts at: 1870 4763 1870 Size: 2893 4545 XmnI GAAnn'nnTTC Cuts at: 5567 5567 Size: 7438 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 5246 276 Size: 53 83 186 936 2404 1308 2468 Fragments arranged by size: 2468 2404 1308 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI