(Circular) (Six-base) MAPSORT of: pFN21AA0008.seq Check: 9576 from: 1 to: 7243 pFN21AA0008 7243 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5053 278 Size: 53 83 186 936 3517 2468 Fragments arranged by size: 3517 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2029 4586 1937 Size: 92 2557 4594 Fragments arranged by size: 4594 2557 92 Acc65I G'GTAC_C Cuts at: 4564 4564 Size: 7243 AclI AA'CG_TT Cuts at: 1563 5370 5743 1563 Size: 3807 373 3063 Fragments arranged by size: 3807 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2984 3915 5306 6690 6958 1632 Size: 1352 931 1391 1384 268 1917 Fragments arranged by size: 1917 1391 1384 1352 931 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7046 AflIII A'CryG_T Cuts at: 1203 3181 4027 6142 7119 1203 Size: 1978 846 2115 977 1327 Fragments arranged by size: 2115 1978 1327 977 846 AgeI A'CCGG_T Cuts at: 6074 6074 Size: 7243 AhdI GACnn_n'nnGTC Cuts at: 3884 5972 3884 Size: 2088 5155 AleI CACnn'nnGTG Cuts at: 1166 3288 1166 Size: 2122 5121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7243 AlwNI CAG_nnn'CTG Cuts at: 2953 3326 3797 4986 6558 2953 Size: 373 471 1189 1572 3638 Fragments arranged by size: 3638 1572 1189 471 373 ApaLI G'TGCA_C Cuts at: 5300 6456 5300 Size: 1156 6087 ApoI r'AATT_y Cuts at: 1493 2178 2818 2917 3078 3214 3572 4236 4267 Size: 685 640 99 161 136 358 664 31 Cuts at: 4267 4359 4538 4552 4778 4930 7039 1493 Size: 92 179 14 226 152 2109 1697 Fragments arranged by size: 2109 1697 685 664 640 358 226 179 161 152 136 99 92 31 14 AseI AT'TA_AT Cuts at: 160 1032 4215 5797 160 Size: 872 3183 1582 1606 Fragments arranged by size: 3183 1606 1582 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7243 AvaI C'yCGr_G Cuts at: 1870 1943 4568 1870 Size: 73 2625 4545 Fragments arranged by size: 4545 2625 73 BamHI G'GATC_C Cuts at: 4573 4940 4573 Size: 367 6876 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2728 4564 6019 618 Size: 325 217 627 941 1836 1455 1842 Fragments arranged by size: 1842 1836 1455 941 627 325 217 BanII G_rGCy'C Cuts at: 1391 4562 1391 Size: 3171 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 2749 2972 4043 928 Size: 971 102 748 223 1071 4128 Fragments arranged by size: 4128 1071 971 748 223 102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5434 5468 1903 Size: 34 3497 34 3678 Fragments arranged by size: 3678 3497 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5135 6345 5135 Size: 1210 6033 BclI T'GATC_A Cuts at: 1548 2266 3585 1548 Size: 718 1319 5206 Fragments arranged by size: 5206 1319 718 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5854 136 Size: 107 122 71 631 605 4182 1525 Fragments arranged by size: 4182 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3397 3517 7238 3397 Size: 120 3721 3402 Fragments arranged by size: 3721 3402 120 BlpI GC'TnA_GC Cuts at: 2424 3276 4660 2424 Size: 852 1384 5007 Fragments arranged by size: 5007 1384 852 Bme1580I G_kGCm'C Cuts at: 1790 5304 6460 1790 Size: 3514 1156 2573 Fragments arranged by size: 3514 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2607 1318 Size: 1289 5954 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3015 4974 5932 Size: 416 53 83 399 24 1598 1959 958 Cuts at: 5932 442 Size: 1753 Fragments arranged by size: 1959 1753 1598 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7243 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2637 4200 2637 Size: 1563 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3299 4478 5903 1239 Size: 2060 1179 1425 2579 Fragments arranged by size: 2579 2060 1425 1179 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2190 2619 3048 3614 5368 6233 6531 2190 Size: 429 429 566 1754 865 298 2902 Fragments arranged by size: 2902 1754 865 566 429 429 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5906 882 Size: 617 4407 2219 Fragments arranged by size: 4407 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5034 493 Size: 711 3830 2702 Fragments arranged by size: 3830 2702 711 BsaBI GATnn'nnATC Cuts at: 7237 7237 Size: 7243 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5050 5432 6067 275 Size: 53 83 186 936 3517 382 635 1451 Fragments arranged by size: 3517 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5676 6074 6348 6495 6906 5676 Size: 398 274 147 411 6013 Fragments arranged by size: 6013 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2268 2298 3019 3049 4527 Size: 30 165 30 865 30 721 30 1478 Cuts at: 4527 4557 1178 Size: 30 3864 Fragments arranged by size: 3864 1478 865 721 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4477 1186 Size: 3291 3952 BseYI C'CCAG_C Cuts at: 6446 6942 6446 Size: 496 6747 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 1163 Size: 573 873 5797 Fragments arranged by size: 5797 873 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4693 5454 6482 1540 Size: 275 186 2692 761 1028 2301 Fragments arranged by size: 2692 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5304 5389 6460 5304 Size: 85 1071 6087 Fragments arranged by size: 6087 1071 85 BsmI GAATG_Cn' Cuts at: 2416 3969 4409 4754 4847 2416 Size: 1553 440 345 93 4812 Fragments arranged by size: 4812 1553 440 345 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4562 5304 5389 6460 1391 Size: 399 2772 742 85 1071 2174 Fragments arranged by size: 2772 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6906 6906 Size: 7243 BspHI T'CATG_A Cuts at: 5132 6862 5132 Size: 1730 5513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2638 2755 4598 844 Size: 1794 117 1843 3489 Fragments arranged by size: 3489 1843 1794 117 BsrBI CCG'CTC Cuts at: 1394 1823 5130 1394 Size: 429 3307 3507 Fragments arranged by size: 3507 3307 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2253 3343 3756 5738 5912 65 Size: 1183 1005 1090 413 1982 174 1396 Fragments arranged by size: 1982 1396 1183 1090 1005 413 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4001 5887 6074 1700 Size: 110 2191 1886 187 2869 Fragments arranged by size: 2869 2191 1886 187 110 BsrGI T'GTAC_A Cuts at: 96 3512 96 Size: 3416 3827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7243 BssSI C'ACGA_G Cuts at: 5303 6315 5303 Size: 1012 6231 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7243 BstBI TT'CG_AA Cuts at: 6047 6047 Size: 7243 BstEII G'GTnAC_C Cuts at: 1184 4502 1184 Size: 3318 3925 BstYI r'GATC_y Cuts at: 1972 3397 3517 4573 4940 5325 5342 6794 6880 Size: 1425 120 1056 367 385 17 1452 86 Cuts at: 6880 6892 7238 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1425 1056 385 367 346 120 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2931 2931 Size: 7243 BtgI C'CryG_G Cuts at: 513 1065 3286 513 Size: 552 2221 4470 Fragments arranged by size: 4470 2221 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6504 Fragments arranged by size: 6504 683 56 BtsI GCAGTG_nn' Cuts at: 4759 5553 5573 4759 Size: 794 20 6429 Fragments arranged by size: 6429 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7243 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7208 DraI TTT'AAA Cuts at: 2359 3042 3189 3219 4547 2359 Size: 683 147 30 1328 5055 Fragments arranged by size: 5055 1328 683 147 30 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6250 809 Size: 507 4934 1802 Fragments arranged by size: 4934 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4690 5579 7208 Size: 54 1425 50 275 18 2860 889 1629 Cuts at: 7208 8 Size: 43 Fragments arranged by size: 2860 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4690 1537 Size: 275 2878 4090 Fragments arranged by size: 4090 2878 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3895 4191 5173 1353 Size: 2542 296 982 3423 Fragments arranged by size: 3423 2542 982 296 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5830 6204 6350 387 Size: 1064 138 4241 374 146 1280 Fragments arranged by size: 4241 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2984 3299 3915 4478 5306 5903 6690 Size: 393 1352 315 616 563 828 597 787 Cuts at: 6690 6958 1239 Size: 268 1524 Fragments arranged by size: 1524 1352 828 787 616 597 563 393 315 268 EcoNI CCTnn'n_nnAGG Cuts at: 3437 3437 Size: 7243 EcoO109I rG'GnC_Cy Cuts at: 3155 3899 3155 Size: 744 6499 EcoRI G'AATT_C Cuts at: 2178 2818 2917 4267 4552 4930 2178 Size: 640 99 1350 285 378 4491 Fragments arranged by size: 4491 1350 640 378 285 99 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2565 2938 4159 2565 Size: 373 1221 5649 Fragments arranged by size: 5649 1221 373 FspI TGC'GCA Cuts at: 5749 5749 Size: 7243 HaeII r_GCGC'y Cuts at: 1678 6390 1678 Size: 4712 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 3870 3902 4145 Size: 32 135 32 614 32 1652 32 243 Cuts at: 4145 4177 4453 4485 5884 5916 5958 5990 1373 Size: 32 276 32 1399 32 42 32 2626 Fragments arranged by size: 2626 1652 1399 614 276 243 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2030 3934 4587 4833 669 Size: 988 281 92 1904 653 246 3079 Fragments arranged by size: 3079 1904 988 653 281 246 92 HindIII A'AGCT_T Cuts at: 748 748 Size: 7243 HpaI GTT'AAC Cuts at: 4833 4833 Size: 7243 KpnI G_GTAC'C Cuts at: 4568 4568 Size: 7243 MfeI C'AATT_G Cuts at: 4842 4842 Size: 7243 MluI A'CGCG_T Cuts at: 7119 7119 Size: 7243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2186 2554 6357 6541 1305 Size: 881 368 3803 184 2007 Fragments arranged by size: 3803 2007 881 368 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5421 Fragments arranged by size: 5421 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3091 3288 5201 5560 5719 518 Size: 648 1925 197 1913 359 159 2042 Fragments arranged by size: 2042 1925 1913 648 359 197 159 MspA1I CmG'CkG Cuts at: 1606 4456 4659 5336 6484 6729 1606 Size: 2850 203 677 1148 245 2120 Fragments arranged by size: 2850 2120 1148 677 245 203 NcoI C'CATG_G Cuts at: 513 1065 3286 513 Size: 552 2221 4470 Fragments arranged by size: 4470 2221 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7243 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7243 NotI GC'GGCC_GC Cuts at: 4690 4690 Size: 7243 NspI r_CATG'y Cuts at: 1132 1229 3185 4031 4481 4601 6146 1132 Size: 97 1956 846 450 120 1545 2229 Fragments arranged by size: 2229 1956 1545 846 450 120 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7243 PciI A'CATG_T Cuts at: 3181 4027 6142 3181 Size: 846 2115 4282 Fragments arranged by size: 4282 2115 846 PflMI CCAn_nnn'nTGG Cuts at: 1108 2957 1108 Size: 1849 5394 PfoI T'CCnGG_A Cuts at: 4093 4093 Size: 7243 PmeI GTTT'AAAC Cuts at: 4547 4547 Size: 7243 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5312 5344 6825 6857 5312 Size: 32 1481 32 5698 Fragments arranged by size: 5698 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3155 3155 Size: 7243 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7243 PsiI TTA'TAA Cuts at: 4813 4813 Size: 7243 PstI C_TGCA'G Cuts at: 830 4439 4595 830 Size: 3609 156 3478 Fragments arranged by size: 3609 3478 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7243 PvuII CAG'CTG Cuts at: 4456 4456 Size: 7243 SalI G'TCGA_C Cuts at: 1936 2028 4585 1936 Size: 92 2557 4594 Fragments arranged by size: 4594 2557 92 SbfI CC_TGCA'GG Cuts at: 4595 4595 Size: 7243 ScaI AGT'ACT Cuts at: 2050 5491 7203 2050 Size: 3441 1712 2090 Fragments arranged by size: 3441 2090 1712 SexAI A'CCwGG_T Cuts at: 2533 4423 2533 Size: 1890 5353 SfcI C'TryA_G Cuts at: 826 1045 4435 4591 5726 6407 6598 826 Size: 219 3390 156 1135 681 191 1471 Fragments arranged by size: 3390 1471 1135 681 219 191 156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7243 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7243 SmaI CCC'GGG Cuts at: 1872 4570 1872 Size: 2698 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2205 2634 3063 3593 5347 6248 Size: 197 926 262 429 429 530 1754 901 Cuts at: 6248 6510 820 Size: 262 1553 Fragments arranged by size: 1754 1553 926 901 530 429 429 262 262 197 SnaBI TAC'GTA Cuts at: 493 5034 493 Size: 4541 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7243 SphI G_CATG'C Cuts at: 1132 4601 1132 Size: 3469 3774 SspI AAT'ATT Cuts at: 5 52 2355 5167 5 Size: 47 2303 2812 2081 Fragments arranged by size: 2812 2303 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7243 StyI C'CwwG_G Cuts at: 513 1065 2166 2240 3286 4682 513 Size: 552 1101 74 1046 1396 3074 Fragments arranged by size: 3074 1396 1101 1046 552 74 SwaI ATTT'AAAT Cuts at: 3219 3219 Size: 7243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3034 3594 5270 5287 5440 5625 6070 Size: 1255 1454 560 1676 17 153 185 445 Cuts at: 6070 325 Size: 1498 Fragments arranged by size: 1676 1498 1454 1255 560 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2048 3512 5489 Size: 274 80 33 51 1443 71 1464 1977 Cuts at: 5489 7201 96 Size: 1712 138 Fragments arranged by size: 1977 1712 1464 1443 274 138 80 71 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3019 3051 3590 3622 7203 7235 3019 Size: 32 539 32 3581 32 3027 Fragments arranged by size: 3581 3027 539 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2535 1316 Size: 1219 6024 XbaI T'CTAG_A Cuts at: 4579 4579 Size: 7243 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2482 2482 Size: 7243 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7243 XmaI C'CCGG_G Cuts at: 1870 4568 1870 Size: 2698 4545 XmnI GAAnn'nnTTC Cuts at: 2921 5372 2921 Size: 2451 4792 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5051 276 Size: 53 83 186 936 3517 2468 Fragments arranged by size: 3517 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI