Restriction map for
pFN21AB7890
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BanI
BbeI BbvCI BciVI BfrI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco52I Eco81I EcoO65I EcoT14I FauI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MluI MroI MspA1I MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflFI PinAI PmeI PshBI
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AclI 1688 AACGTT AA^CGTT
AhdI 565 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 712 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1560 AGCGCT AGC^GCT
ApaI 1213 GGGCCC GGGCC^C
Asp700I 703 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 565 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1695 GAGCAC G[AT]GC[AT]^C
AvaI 123 CTCGGG C^[CT]CG[GA]G
BalI 1346 TGGCCA TGG^CCA
BamHI 1071 GGATCC G^GATCC
BanII 1213 GGGCCC G[GA]GC[CT]^C
BbrPI 1350 CACGTG CAC^GTG
BcgI 750 GCAGAAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 509 TGATCA T^GATCA
BcnI 285 CCGGG CC^[GC]GG
BfaI 1469 CTAG C^TAG
BglI 1518 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 309 AGATCT A^GATCT
BseRI 46 GAGGAG GAGGAG(10/8)
BsiHKAI 1695 GAGCAC G[AT]GC[AT]^C
BsmI 1413 GCATTC GAATGC(1/-1)
BsoBI 123 CTCGGG C^[CT]CG[GA]G
BspHI 162 TCATGA T^CATGA
Cac8I 812 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 1561 GCGC GCG^C
DraI 916 TTTAAA TTT^AAA
DraIII 788 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 113 CCACGG C^C[GA][CT]GG
EaeI 1346 TGGCCA [CT]^GGCC[GA]
Eam1105I 565 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1560 AGCGCT AGC^GCT
EcoNI 39 CCTCTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 707 GAATTC G^AATTC
EcoRV 1813 GATATC GAT^ATC
EcoT22I 1750 ATGCAT ATGCA^T
FbaI 509 TGATCA T^GATCA
HaeII 1560 AGCGCT [GA]GCGC^[CT]
HapII 285 CCGG C^CGG
HhaI 1561 GCGC GCG^C
HinP1I 1561 GCGC G^CGC
HindIII 413 AAGCTT A^AGCTT
HpaII 285 CCGG C^CGG
MaeI 1469 CTAG C^TAG
MfeI 436 CAATTG C^AATTG
MluNI 1346 TGGCCA TGG^CCA
MscI 1346 TGGCCA TGG^CCA
MslI 1188 CACTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 285 CCGG C^CGG
MunI 436 CAATTG C^AATTG
NciI 285 CCGGG CC^[GC]GG
NsiI 1750 ATGCAT ATGCA^T
PflMI 1062 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1350 CACGTG CAC^GTG
PmlI 1350 CACGTG CAC^GTG
PshAI 1130 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1688 AACGTT AA^CGTT
RcaI 162 TCATGA T^CATGA
ScaI 1540 AGTACT AGT^ACT
SexAI 1944 ACCTGGT A^CC[AT]GGT
SfcI 1218 CTACAG C^T[GA][CT]AG
SmlI 1544 CTCAAG C^T[CT][GA]AG
SspI 1623 AATATT AAT^ATT
Van91I 1062 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 703 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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BsaAI 1350 CACGTG [CT]AC^GT[GA]
1824 CACGTA
BsrDI 1445 CATTGC GCAATG(2/0)
1843 GCAATG
BstBI 145 TTCGAA TT^CGAA
1835 TTCGAA
EarI 142 CTCTTC CTCTTC(1/4)
248 GAAGAG
HphI 1810 GGTGA GGTGA(8/7)
101 TCACC
Ksp632I 142 CTCTTC CTCTTC(1/4)
248 GAAGAG
NspV 145 TTCGAA TT^CGAA
1835 TTCGAA
PpuMI 1420 AGGACCC [GA]G^G[AT]CC[CT]
1731 GGGTCCC
SfuI 145 TTCGAA TT^CGAA
1835 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BmyI
BpmI BpuAI BsaI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII