(Circular) (Six-base) MAPSORT of: pFN21AB7875.seq Check: 2850 from: 1 to: 6415 pFN21AB7875 6415 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4225 278 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3758 1937 Size: 1821 4594 Acc65I G'GTAC_C Cuts at: 3736 3736 Size: 6415 AclI AA'CG_TT Cuts at: 1563 4542 4915 1563 Size: 2979 373 3063 Fragments arranged by size: 3063 2979 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 4478 5862 6130 1632 Size: 404 2442 1384 268 1917 Fragments arranged by size: 2442 1917 1384 404 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6218 AflIII A'CryG_T Cuts at: 1203 5314 6291 1203 Size: 4111 977 1327 Fragments arranged by size: 4111 1327 977 AgeI A'CCGG_T Cuts at: 2901 2985 3405 5246 2901 Size: 84 420 1841 4070 Fragments arranged by size: 4070 1841 420 84 AhdI GACnn_n'nnGTC Cuts at: 5144 5144 Size: 6415 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6415 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2068 1184 Size: 884 5531 AlwNI CAG_nnn'CTG Cuts at: 4158 5730 4158 Size: 1572 4843 ApaLI G'TGCA_C Cuts at: 4472 5628 4472 Size: 1156 5259 ApoI r'AATT_y Cuts at: 1493 2132 2567 2679 2695 2763 2931 3200 3267 Size: 639 435 112 16 68 168 269 67 Cuts at: 3267 3724 3950 4102 6211 1493 Size: 457 226 152 2109 1697 Fragments arranged by size: 2109 1697 639 457 435 269 226 168 152 112 68 67 16 AseI AT'TA_AT Cuts at: 160 1032 4969 160 Size: 872 3937 1606 Fragments arranged by size: 3937 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 AvaI C'yCGr_G Cuts at: 1870 1943 3740 1870 Size: 73 1797 4545 Fragments arranged by size: 4545 1797 73 BamHI G'GATC_C Cuts at: 3745 4112 3745 Size: 367 6048 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3736 5191 618 Size: 325 217 627 1949 1455 1842 Fragments arranged by size: 1949 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2286 3734 1391 Size: 895 1448 4072 Fragments arranged by size: 4072 1448 895 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2212 3570 928 Size: 971 313 1358 3773 Fragments arranged by size: 3773 1358 971 313 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4606 4640 1903 Size: 34 2669 34 3678 Fragments arranged by size: 3678 2669 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2040 4307 5517 2040 Size: 2267 1210 2938 Fragments arranged by size: 2938 2267 1210 BclI T'GATC_A Cuts at: 1548 2256 1548 Size: 708 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5026 136 Size: 107 122 71 631 605 3354 1525 Fragments arranged by size: 3354 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6410 6410 Size: 6415 BlpI GC'TnA_GC Cuts at: 2169 3832 2169 Size: 1663 4752 Bme1580I G_kGCm'C Cuts at: 1790 4476 5632 1790 Size: 2686 1156 2573 Fragments arranged by size: 2686 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6415 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2318 4146 5104 Size: 416 53 83 399 24 901 1828 958 Cuts at: 5104 442 Size: 1753 Fragments arranged by size: 1828 1753 958 901 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2043 2162 2793 2877 2961 3045 3129 3213 Size: 804 119 631 84 84 84 84 84 Cuts at: 3213 3297 3381 3465 3633 3715 5075 1239 Size: 84 84 84 168 82 1360 2579 Fragments arranged by size: 2579 1360 804 631 168 119 84 84 84 84 84 84 84 84 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2021 4540 5405 5703 2021 Size: 2519 865 298 2733 Fragments arranged by size: 2733 2519 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5078 882 Size: 617 3579 2219 Fragments arranged by size: 3579 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4206 493 Size: 711 3002 2702 Fragments arranged by size: 3002 2702 711 BsaBI GATnn'nnATC Cuts at: 6409 6409 Size: 6415 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4222 4604 5239 275 Size: 53 83 186 936 2689 382 635 1451 Fragments arranged by size: 2689 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2901 2985 3405 4848 5246 5520 5667 6078 2901 Size: 84 420 1443 398 274 147 411 3238 Fragments arranged by size: 3238 1443 420 411 398 274 147 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6190 Fragments arranged by size: 6190 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 1186 Size: 973 5442 BseYI C'CCAG_C Cuts at: 5618 6114 5618 Size: 496 5919 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 3324 3408 1163 Size: 573 723 865 84 4170 Fragments arranged by size: 4170 865 723 573 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3865 4626 5654 1540 Size: 275 186 1864 761 1028 2301 Fragments arranged by size: 2301 1864 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4476 4561 5632 4476 Size: 85 1071 5259 Fragments arranged by size: 5259 1071 85 BsmI GAATG_Cn' Cuts at: 3926 4019 3926 Size: 93 6322 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 3734 4476 4561 5632 1391 Size: 399 496 1448 742 85 1071 2174 Fragments arranged by size: 2174 1448 1071 742 496 399 85 BspEI T'CCGG_A Cuts at: 6078 6078 Size: 6415 BspHI T'CATG_A Cuts at: 4304 6034 4304 Size: 1730 4685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2219 3770 844 Size: 1375 1551 3489 Fragments arranged by size: 3489 1551 1375 BsrBI CCG'CTC Cuts at: 1394 1823 4302 1394 Size: 429 2479 3507 Fragments arranged by size: 3507 2479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2159 4910 5084 65 Size: 1183 911 2751 174 1396 Fragments arranged by size: 2751 1396 1183 911 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2901 2985 3405 5059 5246 1700 Size: 110 1091 84 420 1654 187 2869 Fragments arranged by size: 2869 1654 1091 420 187 110 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6415 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6415 BssSI C'ACGA_G Cuts at: 4475 5487 4475 Size: 1012 5403 BstAPI GCAn_nnn'nTGC Cuts at: 794 2234 794 Size: 1440 4975 BstBI TT'CG_AA Cuts at: 5219 5219 Size: 6415 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6415 BstYI r'GATC_y Cuts at: 1972 3745 4112 4497 4514 5966 6052 6064 6410 Size: 1773 367 385 17 1452 86 12 346 Cuts at: 6410 1972 Size: 1977 Fragments arranged by size: 1977 1773 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4924 Fragments arranged by size: 4924 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2321 487 Size: 683 56 1095 4581 Fragments arranged by size: 4581 1095 683 56 BtsI GCAGTG_nn' Cuts at: 2163 3931 4725 4745 2163 Size: 1768 794 20 3833 Fragments arranged by size: 3833 1768 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6415 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6380 DraI TTT'AAA Cuts at: 3719 3719 Size: 6415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5422 809 Size: 507 4106 1802 Fragments arranged by size: 4106 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2357 3862 Size: 54 1425 50 275 18 294 233 1505 Cuts at: 3862 4751 6380 8 Size: 889 1629 43 Fragments arranged by size: 1629 1505 1425 889 294 275 233 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3862 1537 Size: 275 2050 4090 Fragments arranged by size: 4090 2050 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 4345 1353 Size: 938 2054 3423 Fragments arranged by size: 3423 2054 938 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5002 5376 5522 387 Size: 1064 138 3413 374 146 1280 Fragments arranged by size: 3413 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2043 2162 2793 2877 2961 3045 Size: 393 404 7 119 631 84 84 84 Cuts at: 3045 3129 3213 3297 3381 3465 3633 3715 4478 Size: 84 84 84 84 84 168 82 763 Cuts at: 4478 5075 5862 6130 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 763 631 597 404 393 268 168 119 84 84 84 84 84 84 84 84 82 7 EcoRI G'AATT_C Cuts at: 2679 2763 2931 3267 3724 4102 2679 Size: 84 168 336 457 378 4992 Fragments arranged by size: 4992 457 378 336 168 84 FspI TGC'GCA Cuts at: 4921 4921 Size: 6415 HaeII r_GCGC'y Cuts at: 1678 5562 1678 Size: 3884 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2248 2280 2299 2331 5056 Size: 32 135 32 676 32 19 32 2725 Cuts at: 5056 5088 5130 5162 1373 Size: 32 42 32 2626 Fragments arranged by size: 2725 2626 676 135 42 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 3759 4005 669 Size: 988 281 1821 246 3079 Fragments arranged by size: 3079 1821 988 281 246 HindIII A'AGCT_T Cuts at: 748 2808 3060 3144 3312 3480 3648 748 Size: 2060 252 84 168 168 168 3515 Fragments arranged by size: 3515 2060 252 168 168 168 84 HpaI GTT'AAC Cuts at: 4005 4005 Size: 6415 KpnI G_GTAC'C Cuts at: 3740 3740 Size: 6415 MfeI C'AATT_G Cuts at: 4014 4014 Size: 6415 MluI A'CGCG_T Cuts at: 6291 6291 Size: 6415 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5529 5713 1305 Size: 4224 184 2007 Fragments arranged by size: 4224 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2126 2359 10 Size: 54 1425 343 294 233 4066 Fragments arranged by size: 4066 1425 343 294 233 54 MslI CAynn'nnrTG Cuts at: 518 1166 4373 4732 4891 518 Size: 648 3207 359 159 2042 Fragments arranged by size: 3207 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3831 4508 5656 5901 1606 Size: 2225 677 1148 245 2120 Fragments arranged by size: 2225 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4924 Fragments arranged by size: 4924 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6415 NotI GC'GGCC_GC Cuts at: 3862 3862 Size: 6415 NspI r_CATG'y Cuts at: 1132 1229 2306 2653 3773 5318 1132 Size: 97 1077 347 1120 1545 2229 Fragments arranged by size: 2229 1545 1120 1077 347 97 PasI CC'CwG_GG Cuts at: 1348 2057 1348 Size: 709 5706 PciI A'CATG_T Cuts at: 5314 5314 Size: 6415 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6415 PmeI GTTT'AAAC Cuts at: 3719 3719 Size: 6415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4484 4516 5997 6029 4484 Size: 32 1481 32 4870 Fragments arranged by size: 4870 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6415 PsiI TTA'TAA Cuts at: 2565 3985 2565 Size: 1420 4995 PstI C_TGCA'G Cuts at: 830 3767 830 Size: 2937 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6415 SalI G'TCGA_C Cuts at: 1936 3757 1936 Size: 1821 4594 SbfI CC_TGCA'GG Cuts at: 3767 3767 Size: 6415 ScaI AGT'ACT Cuts at: 4663 6375 4663 Size: 1712 4703 SexAI A'CCwGG_T Cuts at: 2214 2214 Size: 6415 SfcI C'TryA_G Cuts at: 826 1045 2206 2869 2953 3763 4898 5579 5770 Size: 219 1161 663 84 810 1135 681 191 Cuts at: 5770 826 Size: 1471 Fragments arranged by size: 1471 1161 1135 810 681 663 219 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6415 SmaI CCC'GGG Cuts at: 1872 3742 1872 Size: 1870 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2036 4519 5420 5682 820 Size: 197 926 93 2483 901 262 1553 Fragments arranged by size: 2483 1553 926 901 262 197 93 SnaBI TAC'GTA Cuts at: 493 4206 493 Size: 3713 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6415 SphI G_CATG'C Cuts at: 1132 2653 3773 1132 Size: 1521 1120 3774 Fragments arranged by size: 3774 1521 1120 SspI AAT'ATT Cuts at: 5 52 2532 4339 5 Size: 47 2480 1807 2081 Fragments arranged by size: 2480 2081 1807 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6415 StyI C'CwwG_G Cuts at: 513 1065 2004 3854 513 Size: 552 939 1850 3074 Fragments arranged by size: 3074 1850 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4442 4459 4612 4797 5242 325 Size: 1255 2862 17 153 185 445 1498 Fragments arranged by size: 2862 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2082 4661 6373 Size: 274 80 33 51 1443 105 2579 1712 Cuts at: 6373 96 Size: 138 Fragments arranged by size: 2579 1712 1443 274 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6375 6407 6375 Size: 32 6383 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6415 XbaI T'CTAG_A Cuts at: 2266 3751 2266 Size: 1485 4930 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6415 XmaI C'CCGG_G Cuts at: 1870 3740 1870 Size: 1870 4545 XmnI GAAnn'nnTTC Cuts at: 2532 2615 4544 2532 Size: 83 1929 4403 Fragments arranged by size: 4403 1929 83 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4223 276 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI