(Circular) (Six-base) MAPSORT of: pFN21AB7874.seq Check: 5375 from: 1 to: 6616 pFN21AB7874 6616 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4426 278 Size: 53 83 186 936 2890 2468 Fragments arranged by size: 2890 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3959 1937 Size: 2022 4594 Acc65I G'GTAC_C Cuts at: 2342 2420 3937 2342 Size: 78 1517 5021 Fragments arranged by size: 5021 1517 78 AclI AA'CG_TT Cuts at: 1563 4743 5116 1563 Size: 3180 373 3063 Fragments arranged by size: 3180 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2916 4679 6063 6331 1632 Size: 1284 1763 1384 268 1917 Fragments arranged by size: 1917 1763 1384 1284 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6419 AflIII A'CryG_T Cuts at: 1203 2077 2272 5515 6492 1203 Size: 874 195 3243 977 1327 Fragments arranged by size: 3243 1327 977 874 195 AgeI A'CCGG_T Cuts at: 5447 5447 Size: 6616 AhdI GACnn_n'nnGTC Cuts at: 5345 5345 Size: 6616 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6616 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3666 3705 1184 Size: 2482 39 4095 Fragments arranged by size: 4095 2482 39 AlwNI CAG_nnn'CTG Cuts at: 2606 3176 4359 5931 2606 Size: 570 1183 1572 3291 Fragments arranged by size: 3291 1572 1183 570 ApaLI G'TGCA_C Cuts at: 3038 4673 5829 3038 Size: 1635 1156 3825 Fragments arranged by size: 3825 1635 1156 ApoI r'AATT_y Cuts at: 1493 3925 4151 4303 6412 1493 Size: 2432 226 152 2109 1697 Fragments arranged by size: 2432 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5170 160 Size: 872 4138 1606 Fragments arranged by size: 4138 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6616 AvaI C'yCGr_G Cuts at: 1870 1943 3941 1870 Size: 73 1998 4545 Fragments arranged by size: 4545 1998 73 BamHI G'GATC_C Cuts at: 3946 4313 3946 Size: 367 6249 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2342 2420 3744 3815 3937 Size: 325 217 627 555 78 1324 71 122 Cuts at: 3937 5392 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1324 627 555 325 217 122 78 71 BanII G_rGCy'C Cuts at: 1391 3336 3935 1391 Size: 1945 599 4072 Fragments arranged by size: 4072 1945 599 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3278 928 Size: 971 1379 4266 Fragments arranged by size: 4266 1379 971 BbvCI CC'TCA_GC Cuts at: 3631 3666 3631 Size: 35 6581 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4807 4841 1903 Size: 34 2870 34 3678 Fragments arranged by size: 3678 2870 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3132 4508 5718 3132 Size: 1376 1210 4030 Fragments arranged by size: 4030 1376 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6616 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5227 136 Size: 107 122 71 631 605 3555 1525 Fragments arranged by size: 3555 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2938 3285 6611 2938 Size: 347 3326 2943 Fragments arranged by size: 3326 2943 347 BlpI GC'TnA_GC Cuts at: 4033 4033 Size: 6616 Bme1580I G_kGCm'C Cuts at: 1790 3042 3747 4677 5833 1790 Size: 1252 705 930 1156 2573 Fragments arranged by size: 2573 1252 1156 930 705 BmgBI CAC'GTC Cuts at: 1318 2863 1318 Size: 1545 5071 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2523 4347 5305 Size: 416 53 83 399 24 1106 1824 958 Cuts at: 5305 442 Size: 1753 Fragments arranged by size: 1824 1753 1106 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6616 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2076 5276 1239 Size: 837 3200 2579 Fragments arranged by size: 3200 2579 837 Bpu10I CC'TnA_GC Cuts at: 3631 3666 3631 Size: 35 6581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2747 3522 3687 4741 5606 5904 2747 Size: 775 165 1054 865 298 3459 Fragments arranged by size: 3459 1054 865 775 298 165 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5279 882 Size: 617 3780 2219 Fragments arranged by size: 3780 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3414 4407 493 Size: 711 2210 993 2702 Fragments arranged by size: 2702 2210 993 711 BsaBI GATnn'nnATC Cuts at: 6610 6610 Size: 6616 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4423 4805 5440 275 Size: 53 83 186 936 2890 382 635 1451 Fragments arranged by size: 2890 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5049 5447 5721 5868 6279 5049 Size: 398 274 147 411 5386 Fragments arranged by size: 5386 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2351 2381 1178 Size: 30 165 30 948 30 5413 Fragments arranged by size: 5413 948 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2073 3356 3359 3619 1186 Size: 887 1283 3 260 4183 Fragments arranged by size: 4183 1283 887 260 3 BseYI C'CCAG_C Cuts at: 5819 6315 5819 Size: 496 6120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2492 3061 1163 Size: 573 756 569 4718 Fragments arranged by size: 4718 756 573 569 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3007 4066 4827 5855 1540 Size: 275 186 1006 1059 761 1028 2301 Fragments arranged by size: 2301 1059 1028 1006 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3042 4677 4762 5833 3042 Size: 1635 85 1071 3825 Fragments arranged by size: 3825 1635 1071 85 BsmI GAATG_Cn' Cuts at: 3716 4127 4220 3716 Size: 411 93 6112 Fragments arranged by size: 6112 411 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3042 3336 3747 3935 4677 4762 5833 Size: 399 1252 294 411 188 742 85 1071 Cuts at: 5833 1391 Size: 2174 Fragments arranged by size: 2174 1252 1071 742 411 399 294 188 85 BspEI T'CCGG_A Cuts at: 6279 6279 Size: 6616 BspHI T'CATG_A Cuts at: 4505 6235 4505 Size: 1730 4886 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2492 2591 3971 844 Size: 1648 99 1380 3489 Fragments arranged by size: 3489 1648 1380 99 BsrBI CCG'CTC Cuts at: 1394 1823 4503 1394 Size: 429 2680 3507 Fragments arranged by size: 3507 2680 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5111 5285 65 Size: 1183 3863 174 1396 Fragments arranged by size: 3863 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5260 5447 1700 Size: 110 3450 187 2869 Fragments arranged by size: 3450 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3167 3213 96 Size: 3071 46 3499 Fragments arranged by size: 3499 3071 46 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6616 BssSI C'ACGA_G Cuts at: 4676 5688 4676 Size: 1012 5604 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6616 BstBI TT'CG_AA Cuts at: 2994 5420 2994 Size: 2426 4190 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6616 BstXI CCAn_nnnn'nTGG Cuts at: 3574 3574 Size: 6616 BstYI r'GATC_y Cuts at: 1972 2938 3285 3946 4313 4698 4715 6167 6253 Size: 966 347 661 367 385 17 1452 86 Cuts at: 6253 6265 6611 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 966 661 385 367 347 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2109 513 Size: 552 1044 5020 Fragments arranged by size: 5020 1044 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5877 Fragments arranged by size: 5877 683 56 BtsI GCAGTG_nn' Cuts at: 2451 4132 4926 4946 2451 Size: 1681 794 20 4121 Fragments arranged by size: 4121 1681 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6616 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6581 DraI TTT'AAA Cuts at: 3920 3920 Size: 6616 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5623 809 Size: 507 4307 1802 Fragments arranged by size: 4307 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2227 3297 4063 Size: 54 1425 50 275 18 397 1070 766 Cuts at: 4063 4952 6581 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1070 889 766 397 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4063 1537 Size: 275 2251 4090 Fragments arranged by size: 4090 2251 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4546 1353 Size: 3193 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5203 5577 5723 387 Size: 1064 138 3614 374 146 1280 Fragments arranged by size: 3614 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 2916 4679 5276 6063 6331 1239 Size: 393 444 840 1763 597 787 268 1524 Fragments arranged by size: 1763 1524 840 787 597 444 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2058 2058 Size: 6616 EcoO109I rG'GnC_Cy Cuts at: 2976 3402 2976 Size: 426 6190 EcoRI G'AATT_C Cuts at: 3925 4303 3925 Size: 378 6238 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3007 3410 3007 Size: 403 6213 FspI TGC'GCA Cuts at: 5122 5122 Size: 6616 HaeII r_GCGC'y Cuts at: 1678 5763 1678 Size: 4085 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5257 5289 5331 5363 1373 Size: 32 135 32 3685 32 42 32 2626 Fragments arranged by size: 3685 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3488 3529 3960 4206 669 Size: 988 281 1550 41 431 246 3079 Fragments arranged by size: 3079 1550 988 431 281 246 41 HindIII A'AGCT_T Cuts at: 748 2149 748 Size: 1401 5215 HpaI GTT'AAC Cuts at: 4206 4206 Size: 6616 KpnI G_GTAC'C Cuts at: 2346 2424 3941 2346 Size: 78 1517 5021 Fragments arranged by size: 5021 1517 78 MfeI C'AATT_G Cuts at: 2526 3693 4215 2526 Size: 1167 522 4927 Fragments arranged by size: 4927 1167 522 MluI A'CGCG_T Cuts at: 6492 6492 Size: 6616 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2223 3888 3910 5730 5914 1305 Size: 918 1665 22 1820 184 2007 Fragments arranged by size: 2007 1820 1665 918 184 22 MscI TGG'CCA Cuts at: 10 64 1489 1832 2229 3299 10 Size: 54 1425 343 397 1070 3327 Fragments arranged by size: 3327 1425 1070 397 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2713 2893 3572 4574 4933 5092 518 Size: 648 1547 180 679 1002 359 159 2042 Fragments arranged by size: 2042 1547 1002 679 648 359 180 159 MspA1I CmG'CkG Cuts at: 1606 2410 4032 4709 5857 6102 1606 Size: 804 1622 677 1148 245 2120 Fragments arranged by size: 2120 1622 1148 804 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6064 NdeI CA'TA_TG Cuts at: 387 3160 387 Size: 2773 3843 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6616 NotI GC'GGCC_GC Cuts at: 4063 4063 Size: 6616 NspI r_CATG'y Cuts at: 1132 1229 2081 2276 2666 2816 3527 3550 3974 Size: 97 852 195 390 150 711 23 424 Cuts at: 3974 5519 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 852 711 424 390 195 150 97 23 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6616 PciI A'CATG_T Cuts at: 2077 2272 5515 2077 Size: 195 3243 3178 Fragments arranged by size: 3243 3178 195 PflMI CCAn_nnn'nTGG Cuts at: 1108 3092 1108 Size: 1984 4632 PfoI T'CCnGG_A Cuts at: 2053 2053 Size: 6616 PmeI GTTT'AAAC Cuts at: 3920 3920 Size: 6616 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4685 4717 6198 6230 4685 Size: 32 1481 32 5071 Fragments arranged by size: 5071 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3402 3402 Size: 6616 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6616 PsiI TTA'TAA Cuts at: 3123 4186 3123 Size: 1063 5553 PstI C_TGCA'G Cuts at: 830 3968 830 Size: 3138 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6616 PvuII CAG'CTG Cuts at: 2410 2410 Size: 6616 SalI G'TCGA_C Cuts at: 1936 3958 1936 Size: 2022 4594 SanDI GG'GwC_CC Cuts at: 3402 3402 Size: 6616 SbfI CC_TGCA'GG Cuts at: 3968 3968 Size: 6616 ScaI AGT'ACT Cuts at: 4864 6576 4864 Size: 1712 4904 SfcI C'TryA_G Cuts at: 826 1045 2942 3964 5099 5780 5971 826 Size: 219 1897 1022 1135 681 191 1471 Fragments arranged by size: 1897 1471 1135 1022 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6616 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6616 SmaI CCC'GGG Cuts at: 1872 3943 1872 Size: 2071 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 3537 3702 4720 5621 5883 Size: 197 926 819 775 165 1018 901 262 Cuts at: 5883 820 Size: 1553 Fragments arranged by size: 1553 1018 926 901 819 775 262 197 165 SnaBI TAC'GTA Cuts at: 493 3414 4407 493 Size: 2921 993 2702 Fragments arranged by size: 2921 2702 993 SpeI A'CTAG_T Cuts at: 152 152 Size: 6616 SphI G_CATG'C Cuts at: 1132 2816 3974 1132 Size: 1684 1158 3774 Fragments arranged by size: 3774 1684 1158 SspI AAT'ATT Cuts at: 5 52 3203 4540 5 Size: 47 3151 1337 2081 Fragments arranged by size: 3151 2081 1337 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6616 StyI C'CwwG_G Cuts at: 513 1065 4055 513 Size: 552 2990 3074 Fragments arranged by size: 3074 2990 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2997 3168 3833 4643 4660 4813 4998 Size: 1255 1417 171 665 810 17 153 185 Cuts at: 4998 5443 325 Size: 445 1498 Fragments arranged by size: 1498 1417 1255 810 665 445 185 171 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2326 2912 3167 Size: 274 80 33 51 1443 349 586 255 Cuts at: 3167 3213 4862 6574 96 Size: 46 1649 1712 138 Fragments arranged by size: 1712 1649 1443 586 349 274 255 138 80 51 46 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2351 2383 3224 3256 6576 6608 2351 Size: 32 841 32 3320 32 2359 Fragments arranged by size: 3320 2359 841 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3483 1316 Size: 2167 4449 XbaI T'CTAG_A Cuts at: 2308 3878 3952 2308 Size: 1570 74 4972 Fragments arranged by size: 4972 1570 74 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2548 2548 Size: 6616 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6616 XmaI C'CCGG_G Cuts at: 1870 3941 1870 Size: 2071 4545 XmnI GAAnn'nnTTC Cuts at: 3390 4745 3390 Size: 1355 5261 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4424 276 Size: 53 83 186 936 2890 2468 Fragments arranged by size: 2890 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI