Restriction map for
pFN21AB7874
(Linear) (Six-base) MAPSORT of: KIBB7874.seq Check: 1031 from: 1 to: 1911
KIBB7874.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
Acc65I G'GTAC_C
Cuts at: 0 337 415 1911
Size: 337 78 1496
Fragments arranged by size:
1496 337 78
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1911
Size: 911 1000
AflIII A'CryG_T
Cuts at: 0 72 267 1911
Size: 72 195 1644
Fragments arranged by size:
1644 195 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1661 1700 1911
Size: 1661 39 211
Fragments arranged by size:
1661 211 39
AlwNI CAG_nnn'CTG
Cuts at: 0 601 1171 1911
Size: 601 570 740
Fragments arranged by size:
740 601 570
ApaLI G'TGCA_C
Cuts at: 0 1033 1911
Size: 1033 878
BanI G'GyrC_C
Cuts at: 0 337 415 1739 1810 1911
Size: 337 78 1324 71 101
Fragments arranged by size:
1324 337 101 78 71
BanII G_rGCy'C
Cuts at: 0 1331 1911
Size: 1331 580
BbsI GAAGACnn'nnnn_
Cuts at: 0 1273 1911
Size: 1273 638
BbvCI CC'TCA_GC
Cuts at: 0 1626 1661 1911
Size: 1626 35 250
Fragments arranged by size:
1626 250 35
BciVI GTATCCnnnnn_n'
Cuts at: 0 1127 1911
Size: 1127 784
BglII A'GATC_T
Cuts at: 0 933 1280 1911
Size: 933 347 631
Fragments arranged by size:
933 631 347
Bme1580I G_kGCm'C
Cuts at: 0 1037 1742 1911
Size: 1037 705 169
Fragments arranged by size:
1037 705 169
BmgBI CAC'GTC
Cuts at: 0 858 1911
Size: 858 1053
BmrI ACTGGGnnnn_n'
Cuts at: 0 518 1911
Size: 518 1393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1911
Size: 71 1840
Bpu10I CC'TnA_GC
Cuts at: 0 1626 1661 1911
Size: 1626 35 250
Fragments arranged by size:
1626 250 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1517 1682 1911
Size: 742 775 165 229
Fragments arranged by size:
775 742 229 165
BsaAI yAC'GTr
Cuts at: 0 1409 1911
Size: 1409 502
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 346 376 1911
Size: 346 30 1535
Fragments arranged by size:
1535 346 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 68 1351 1354 1614 1911
Size: 68 1283 3 260 297
Fragments arranged by size:
1283 297 260 68 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1056 1911
Size: 487 569 855
Fragments arranged by size:
855 569 487
BsiEI CG_ry'CG
Cuts at: 0 1002 1911
Size: 1002 909
BsiHKAI G_wGCw'C
Cuts at: 0 1037 1911
Size: 1037 874
BsmI GAATG_Cn'
Cuts at: 0 1711 1911
Size: 1711 200
Bsp1286I G_dGCh'C
Cuts at: 0 1037 1331 1742 1911
Size: 1037 294 411 169
Fragments arranged by size:
1037 411 294 169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 487 586 1911
Size: 487 99 1325
Fragments arranged by size:
1325 487 99
BsrGI T'GTAC_A
Cuts at: 0 1162 1208 1911
Size: 1162 46 703
Fragments arranged by size:
1162 703 46
BstBI TT'CG_AA
Cuts at: 0 989 1911
Size: 989 922
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1569 1911
Size: 1569 342
BstYI r'GATC_y
Cuts at: 0 933 1280 1911
Size: 933 347 631
Fragments arranged by size:
933 631 347
BtgI C'CryG_G
Cuts at: 0 104 1911
Size: 104 1807
BtsI GCAGTG_nn'
Cuts at: 0 446 1911
Size: 446 1465
EaeI y'GGCC_r
Cuts at: 0 222 1292 1911
Size: 222 1070 619
Fragments arranged by size:
1070 619 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 911 1911
Size: 71 840 1000
Fragments arranged by size:
1000 840 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 53 1911
Size: 53 1858
EcoO109I rG'GnC_Cy
Cuts at: 0 971 1397 1911
Size: 971 426 514
Fragments arranged by size:
971 514 426
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1002 1405 1911
Size: 1002 403 506
Fragments arranged by size:
1002 506 403
HincII GTy'rAC
Cuts at: 0 1483 1524 1911
Size: 1483 41 387
Fragments arranged by size:
1483 387 41
HindIII A'AGCT_T
Cuts at: 0 144 1911
Size: 144 1767
KpnI G_GTAC'C
Cuts at: 0 341 419 1911
Size: 341 78 1492
Fragments arranged by size:
1492 341 78
MfeI C'AATT_G
Cuts at: 0 521 1688 1911
Size: 521 1167 223
Fragments arranged by size:
1167 521 223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1883 1905 1911
Size: 218 1665 22 6
Fragments arranged by size:
1665 218 22 6
MscI TGG'CCA
Cuts at: 0 224 1294 1911
Size: 224 1070 617
Fragments arranged by size:
1070 617 224
MslI CAynn'nnrTG
Cuts at: 0 708 888 1567 1911
Size: 708 180 679 344
Fragments arranged by size:
708 679 344 180
MspA1I CmG'CkG
Cuts at: 0 405 1911
Size: 405 1506
NdeI CA'TA_TG
Cuts at: 0 1155 1911
Size: 1155 756
NspI r_CATG'y
Cuts at: 0 76 271 661 811 1522 1545 1911
Size: 76 195 390 150 711 23 366
Fragments arranged by size:
711 390 366 195 150 76 23
PciI A'CATG_T
Cuts at: 0 72 267 1911
Size: 72 195 1644
Fragments arranged by size:
1644 195 72
PflMI CCAn_nnn'nTGG
Cuts at: 0 1087 1911
Size: 1087 824
PfoI T'CCnGG_A
Cuts at: 0 48 1911
Size: 48 1863
PpuMI rG'GwC_Cy
Cuts at: 0 1397 1911
Size: 1397 514
PsiI TTA'TAA
Cuts at: 0 1118 1911
Size: 1118 793
PvuII CAG'CTG
Cuts at: 0 405 1911
Size: 405 1506
SanDI GG'GwC_CC
Cuts at: 0 1397 1911
Size: 1397 514
SfcI C'TryA_G
Cuts at: 0 937 1911
Size: 937 974
SmlI C'TyrA_G
Cuts at: 0 757 1532 1697 1911
Size: 757 775 165 214
Fragments arranged by size:
775 757 214 165
SnaBI TAC'GTA
Cuts at: 0 1409 1911
Size: 1409 502
SphI G_CATG'C
Cuts at: 0 811 1911
Size: 811 1100
SspI AAT'ATT
Cuts at: 0 1198 1911
Size: 1198 713
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 992 1163 1828 1911
Size: 992 171 665 83
Fragments arranged by size:
992 665 171 83
TatI w'GTAC_w
Cuts at: 0 321 907 1162 1208 1911
Size: 321 586 255 46 703
Fragments arranged by size:
703 586 321 255 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 346 378 1219 1251 1911
Size: 346 32 841 32 660
Fragments arranged by size:
841 660 346 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1478 1911
Size: 1478 433
XbaI T'CTAG_A
Cuts at: 0 303 1873 1911
Size: 303 1570 38
Fragments arranged by size:
1570 303 38
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 543 1911
Size: 543 1368
XmnI GAAnn'nnTTC
Cuts at: 0 1385 1911
Size: 1385 526
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AlwNI ApaLI BanI BanII
BbsI BbvCI BciVI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaAI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrGI BstBI BstXI BstYI BtgI
BtsI EaeI Eco57MI EcoNI EcoO109I FalI HincII HindIII
KpnI MfeI MmeI MscI MslI MspA1I NdeI NspI
PciI PflMI PfoI PpuMI PsiI PvuII SanDI SfcI
SmlI SnaBI SphI SspI TaqII TatI TstI Tth111I
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglI
BlpI BmtI BplI BsaI BsaBI BsaHI BsaWI BseYI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HpaI KasI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SrfI StuI StyI SwaI XhoI XmaI
ZraI