(Circular) (Six-base) MAPSORT of: pFN21AB7864.seq Check: 8061 from: 1 to: 6598 pFN21AB7864 6598 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2941 3744 3840 2941 Size: 803 96 5699 Fragments arranged by size: 5699 803 96 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4408 278 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2796 3038 3941 1937 Size: 859 242 903 4594 Fragments arranged by size: 4594 903 859 242 Acc65I G'GTAC_C Cuts at: 3919 3919 Size: 6598 AclI AA'CG_TT Cuts at: 1563 4725 5098 1563 Size: 3162 373 3063 Fragments arranged by size: 3162 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4661 6045 6313 1632 Size: 3029 1384 268 1917 Fragments arranged by size: 3029 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6401 AflIII A'CryG_T Cuts at: 1203 2856 5497 6474 1203 Size: 1653 2641 977 1327 Fragments arranged by size: 2641 1653 1327 977 AgeI A'CCGG_T Cuts at: 5429 5429 Size: 6598 AhdI GACnn_n'nnGTC Cuts at: 5327 5327 Size: 6598 AleI CACnn'nnGTG Cuts at: 1166 2786 1166 Size: 1620 4978 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3806 1184 Size: 2622 3976 AlwNI CAG_nnn'CTG Cuts at: 2092 2644 2764 3122 3406 3644 3736 4341 5913 Size: 552 120 358 284 238 92 605 1572 Cuts at: 5913 2092 Size: 2777 Fragments arranged by size: 2777 1572 605 552 358 284 238 120 92 ApaI G_GGCC'C Cuts at: 2109 2627 2871 3131 2109 Size: 518 244 260 5576 Fragments arranged by size: 5576 518 260 244 ApaLI G'TGCA_C Cuts at: 4655 5811 4655 Size: 1156 5442 ApoI r'AATT_y Cuts at: 1493 3907 4133 4285 6394 1493 Size: 2414 226 152 2109 1697 Fragments arranged by size: 2414 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5152 160 Size: 872 4120 1606 Fragments arranged by size: 4120 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 AvaI C'yCGr_G Cuts at: 1870 1943 2205 2887 3524 3923 1870 Size: 73 262 682 637 399 4545 Fragments arranged by size: 4545 682 637 399 262 73 BamHI G'GATC_C Cuts at: 3928 4295 3928 Size: 367 6231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3293 3919 5374 618 Size: 325 217 627 1506 626 1455 1842 Fragments arranged by size: 1842 1506 1455 627 626 325 217 BanII G_rGCy'C Cuts at: 1391 2040 2109 2213 2571 2627 2771 2871 3078 Size: 649 69 104 358 56 144 100 207 Cuts at: 3078 3131 3500 3806 3917 1391 Size: 53 369 306 111 4072 Fragments arranged by size: 4072 649 369 358 306 207 144 111 104 100 69 56 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2247 2474 3622 928 Size: 971 348 227 1148 3904 Fragments arranged by size: 3904 1148 971 348 227 BbvCI CC'TCA_GC Cuts at: 2279 2279 Size: 6598 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4789 4823 1903 Size: 34 2852 34 3678 Fragments arranged by size: 3678 2852 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4490 5700 4490 Size: 1210 5388 BclI T'GATC_A Cuts at: 1548 1548 Size: 6598 BfrBI ATG'CAT Cuts at: 2727 2727 Size: 6598 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2191 3482 5209 Size: 107 122 71 631 605 519 1291 1727 Cuts at: 5209 136 Size: 1525 Fragments arranged by size: 1727 1525 1291 631 605 519 122 107 71 BglII A'GATC_T Cuts at: 6593 6593 Size: 6598 BlpI GC'TnA_GC Cuts at: 2066 2178 3486 3755 4015 2066 Size: 112 1308 269 260 4649 Fragments arranged by size: 4649 1308 269 260 112 Bme1580I G_kGCm'C Cuts at: 1790 2109 2459 2627 2871 3131 4659 5815 1790 Size: 319 350 168 244 260 1528 1156 2573 Fragments arranged by size: 2573 1528 1156 350 319 260 244 168 BmgBI CAC'GTC Cuts at: 1318 3703 1318 Size: 2385 4213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2102 2940 3529 Size: 416 53 83 399 24 685 838 589 Cuts at: 3529 4329 5287 442 Size: 800 958 1753 Fragments arranged by size: 1753 958 838 800 685 589 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6598 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3280 3280 Size: 6598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2075 2142 2291 3092 3637 3763 3796 Size: 830 6 67 149 801 545 126 33 Cuts at: 3796 5258 1239 Size: 1462 2579 Fragments arranged by size: 2579 1462 830 801 545 149 126 67 33 6 Bpu10I CC'TnA_GC Cuts at: 2279 3593 2279 Size: 1314 5284 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2992 4723 5588 5886 2992 Size: 1731 865 298 3704 Fragments arranged by size: 3704 1731 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2312 3118 3854 5261 882 Size: 617 813 806 736 1407 2219 Fragments arranged by size: 2219 1407 813 806 736 617 BsaAI yAC'GTr Cuts at: 493 1204 2859 4389 493 Size: 711 1655 1530 2702 Fragments arranged by size: 2702 1655 1530 711 BsaBI GATnn'nnATC Cuts at: 6592 6592 Size: 6598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4405 4787 5422 275 Size: 53 83 186 936 2872 382 635 1451 Fragments arranged by size: 2872 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5031 5429 5703 5850 6261 5031 Size: 398 274 147 411 5368 Fragments arranged by size: 5368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2324 2354 2596 2626 1178 Size: 30 165 30 921 30 242 30 5150 Fragments arranged by size: 5150 921 242 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 2699 2720 1186 Size: 1117 396 21 5064 Fragments arranged by size: 5064 1117 396 21 BseYI C'CCAG_C Cuts at: 2194 2626 2634 2764 3077 3122 3399 3707 5801 Size: 432 8 130 313 45 277 308 2094 Cuts at: 5801 6297 2194 Size: 496 2495 Fragments arranged by size: 2495 2094 496 432 313 308 277 130 45 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3146 3392 3464 1163 Size: 573 1410 246 72 4297 Fragments arranged by size: 4297 1410 573 246 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2164 4048 4809 5837 1540 Size: 275 186 163 1884 761 1028 2301 Fragments arranged by size: 2301 1884 1028 761 275 186 163 BsiHKAI G_wGCw'C Cuts at: 2040 3806 4659 4744 5815 2040 Size: 1766 853 85 1071 2823 Fragments arranged by size: 2823 1766 1071 853 85 BsmI GAATG_Cn' Cuts at: 2729 4109 4202 2729 Size: 1380 93 5125 Fragments arranged by size: 5125 1380 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2216 2687 2216 Size: 471 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2109 2213 2459 2571 2627 2771 Size: 399 250 69 104 246 112 56 144 Cuts at: 2771 2871 3078 3131 3500 3806 3917 4659 4744 Size: 100 207 53 369 306 111 742 85 Cuts at: 4744 5815 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 306 250 246 207 144 112 111 104 100 85 69 56 53 BspEI T'CCGG_A Cuts at: 6261 6261 Size: 6598 BspHI T'CATG_A Cuts at: 3237 4487 6217 3237 Size: 1250 1730 3618 Fragments arranged by size: 3618 1730 1250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2379 2902 2941 3616 3744 3840 3953 844 Size: 1535 523 39 675 128 96 113 3489 Fragments arranged by size: 3489 1535 675 523 128 113 96 39 BsrBI CCG'CTC Cuts at: 1394 1823 2906 3430 4485 1394 Size: 429 1083 524 1055 3507 Fragments arranged by size: 3507 1083 1055 524 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3823 5093 5267 65 Size: 1183 2575 1270 174 1396 Fragments arranged by size: 2575 1396 1270 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2814 3343 3676 3722 3742 3750 5242 Size: 110 1004 529 333 46 20 8 1492 Cuts at: 5242 5429 1700 Size: 187 2869 Fragments arranged by size: 2869 1492 1004 529 333 187 110 46 20 8 BsrGI T'GTAC_A Cuts at: 96 2490 96 Size: 2394 4204 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6598 BssSI C'ACGA_G Cuts at: 4658 5670 4658 Size: 1012 5586 BstAPI GCAn_nnn'nTGC Cuts at: 794 3568 794 Size: 2774 3824 BstBI TT'CG_AA Cuts at: 5402 5402 Size: 6598 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6598 BstXI CCAn_nnnn'nTGG Cuts at: 2194 3070 3787 3880 2194 Size: 876 717 93 4912 Fragments arranged by size: 4912 876 717 93 BstYI r'GATC_y Cuts at: 1972 2640 3928 4295 4680 4697 6149 6235 6247 Size: 668 1288 367 385 17 1452 86 12 Cuts at: 6247 6593 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1288 668 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2286 2286 Size: 6598 BtgI C'CryG_G Cuts at: 513 1065 2004 2310 2339 2850 2956 3063 3480 Size: 552 939 306 29 511 106 107 417 Cuts at: 3480 513 Size: 3631 Fragments arranged by size: 3631 939 552 511 417 306 107 106 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5859 Fragments arranged by size: 5859 683 56 BtsI GCAGTG_nn' Cuts at: 2078 3158 4114 4908 4928 2078 Size: 1080 956 794 20 3748 Fragments arranged by size: 3748 1080 956 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6563 DraI TTT'AAA Cuts at: 3902 3902 Size: 6598 DraIII CAC_nnn'GTG Cuts at: 2096 2962 3055 3338 2096 Size: 866 93 283 5356 Fragments arranged by size: 5356 866 283 93 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5605 809 Size: 507 4289 1802 Fragments arranged by size: 4289 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2847 3474 3603 Size: 54 1425 50 275 18 1017 627 129 Cuts at: 3603 3820 4045 4934 6563 8 Size: 217 225 889 1629 43 Fragments arranged by size: 1629 1425 1017 889 627 275 225 217 129 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4045 1537 Size: 275 2233 4090 Fragments arranged by size: 4090 2233 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2559 3892 4528 1353 Size: 1206 1333 636 3423 Fragments arranged by size: 3423 1333 1206 636 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2617 2735 5185 5559 5705 387 Size: 1064 138 1028 118 2450 374 146 1280 Fragments arranged by size: 2450 1280 1064 1028 374 146 138 118 EcoICRI GAG'CTC Cuts at: 2038 3804 2038 Size: 1766 4832 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2075 2142 2291 3092 3637 3763 Size: 393 437 6 67 149 801 545 126 Cuts at: 3763 3796 4661 5258 6045 6313 1239 Size: 33 865 597 787 268 1524 Fragments arranged by size: 1524 865 801 787 597 545 437 393 268 149 126 67 33 6 EcoNI CCTnn'n_nnAGG Cuts at: 2138 2327 2450 2648 2659 2138 Size: 189 123 198 11 6077 Fragments arranged by size: 6077 198 189 123 11 EcoO109I rG'GnC_Cy Cuts at: 2105 2106 2129 2335 2623 2868 2891 3127 3128 Size: 1 23 206 288 245 23 236 1 Cuts at: 3128 3272 3715 2105 Size: 144 443 4988 Fragments arranged by size: 4988 443 288 245 236 206 144 23 23 1 1 EcoRI G'AATT_C Cuts at: 3907 4285 3907 Size: 378 6220 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3646 3646 Size: 6598 FspI TGC'GCA Cuts at: 5104 5104 Size: 6598 HaeII r_GCGC'y Cuts at: 1678 3600 3762 5745 1678 Size: 1922 162 1983 2531 Fragments arranged by size: 2531 1983 1922 162 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3164 3196 5239 5271 5313 Size: 32 135 32 1592 32 2043 32 42 Cuts at: 5313 5345 1373 Size: 32 2626 Fragments arranged by size: 2626 2043 1592 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3942 4188 669 Size: 988 281 2004 246 3079 Fragments arranged by size: 3079 2004 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6598 HpaI GTT'AAC Cuts at: 4188 4188 Size: 6598 KpnI G_GTAC'C Cuts at: 3923 3923 Size: 6598 MfeI C'AATT_G Cuts at: 4197 4197 Size: 6598 MluI A'CGCG_T Cuts at: 6474 6474 Size: 6598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2796 3867 5712 5896 1305 Size: 1491 1071 1845 184 2007 Fragments arranged by size: 2007 1845 1491 1071 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2849 3476 3605 3822 10 Size: 54 1425 343 1017 627 129 217 2786 Fragments arranged by size: 2786 1425 1017 627 343 217 129 54 MslI CAynn'nnrTG Cuts at: 518 1166 2462 2786 3878 4556 4915 5074 518 Size: 648 1296 324 1092 678 359 159 2042 Fragments arranged by size: 2042 1296 1092 678 648 359 324 159 MspA1I CmG'CkG Cuts at: 1606 2026 2395 2865 3015 3122 3406 3490 4014 Size: 420 369 470 150 107 284 84 524 Cuts at: 4014 4691 5839 6084 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 524 470 420 369 284 245 150 107 84 NaeI GCC'GGC Cuts at: 3678 3724 3744 3678 Size: 46 20 6532 Fragments arranged by size: 6532 46 20 NcoI C'CATG_G Cuts at: 513 1065 2004 2850 3480 513 Size: 552 939 846 630 3631 Fragments arranged by size: 3631 939 846 630 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6598 NgoMIV G'CCGG_C Cuts at: 3676 3722 3742 3676 Size: 46 20 6532 Fragments arranged by size: 6532 46 20 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6598 NotI GC'GGCC_GC Cuts at: 4045 4045 Size: 6598 NsiI A_TGCA'T Cuts at: 2729 2729 Size: 6598 NspI r_CATG'y Cuts at: 1132 1229 3956 5501 1132 Size: 97 2727 1545 2229 Fragments arranged by size: 2727 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2876 3268 1348 Size: 1528 392 4678 Fragments arranged by size: 4678 1528 392 PciI A'CATG_T Cuts at: 5497 5497 Size: 6598 PflMI CCAn_nnn'nTGG Cuts at: 1108 2092 1108 Size: 984 5614 PmeI GTTT'AAAC Cuts at: 3902 3902 Size: 6598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4667 4699 6180 6212 4667 Size: 32 1481 32 5053 Fragments arranged by size: 5053 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2335 2891 3272 3715 2335 Size: 556 381 443 5218 Fragments arranged by size: 5218 556 443 381 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6598 PsiI TTA'TAA Cuts at: 4168 4168 Size: 6598 PspOMI G'GGCC_C Cuts at: 2105 2623 2867 3127 2105 Size: 518 244 260 5576 Fragments arranged by size: 5576 518 260 244 PstI C_TGCA'G Cuts at: 830 2031 2331 2376 2998 3495 3741 3950 830 Size: 1201 300 45 622 497 246 209 3478 Fragments arranged by size: 3478 1201 622 497 300 246 209 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6598 PvuII CAG'CTG Cuts at: 2026 3122 3490 2026 Size: 1096 368 5134 Fragments arranged by size: 5134 1096 368 SacI G_AGCT'C Cuts at: 2040 3806 2040 Size: 1766 4832 SalI G'TCGA_C Cuts at: 1936 3940 1936 Size: 2004 4594 SapI GCTCTTCn'nnn_ Cuts at: 2559 2559 Size: 6598 SbfI CC_TGCA'GG Cuts at: 3950 3950 Size: 6598 ScaI AGT'ACT Cuts at: 4846 6558 4846 Size: 1712 4886 SexAI A'CCwGG_T Cuts at: 2495 2495 Size: 6598 SfcI C'TryA_G Cuts at: 826 1045 2027 2327 2372 2994 3491 3511 3737 Size: 219 982 300 45 622 497 20 226 Cuts at: 3737 3946 5081 5762 5953 826 Size: 209 1135 681 191 1471 Fragments arranged by size: 1471 1135 982 681 622 497 300 226 219 209 191 45 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6598 SmaI CCC'GGG Cuts at: 1872 2889 3925 1872 Size: 1017 1036 4545 Fragments arranged by size: 4545 1036 1017 SmlI C'TyrA_G Cuts at: 820 1017 1943 3007 4702 5603 5865 820 Size: 197 926 1064 1695 901 262 1553 Fragments arranged by size: 1695 1553 1064 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4389 493 Size: 3896 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6598 SphI G_CATG'C Cuts at: 1132 3956 1132 Size: 2824 3774 SspI AAT'ATT Cuts at: 5 52 4522 5 Size: 47 4470 2081 Fragments arranged by size: 4470 2081 47 StuI AGG'CCT Cuts at: 1517 2654 3189 3650 1517 Size: 1137 535 461 4465 Fragments arranged by size: 4465 1137 535 461 StyI C'CwwG_G Cuts at: 513 1065 2004 2850 3480 4037 513 Size: 552 939 846 630 557 3074 Fragments arranged by size: 3074 939 846 630 557 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 2269 2552 3557 3890 4625 4642 Size: 1255 504 185 283 1005 333 735 17 Cuts at: 4642 4795 4980 5425 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1005 735 504 445 333 283 185 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2490 3095 3537 Size: 274 80 33 51 1443 513 605 442 Cuts at: 3537 4844 6556 96 Size: 1307 1712 138 Fragments arranged by size: 1712 1443 1307 605 513 442 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2324 2356 3041 3073 6558 6590 2324 Size: 32 685 32 3485 32 2332 Fragments arranged by size: 3485 2332 685 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3034 1316 Size: 1718 4880 XbaI T'CTAG_A Cuts at: 3934 3934 Size: 6598 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6598 XmaI C'CCGG_G Cuts at: 1870 2887 3923 1870 Size: 1017 1036 4545 Fragments arranged by size: 4545 1036 1017 XmnI GAAnn'nnTTC Cuts at: 2612 3467 4727 2612 Size: 855 1260 4483 Fragments arranged by size: 4483 1260 855 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4406 276 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI