Restriction map for
pFN21AB7661
(Circular) (Six-base) MAPSORT of: pFN21AB7661.seq Check: 1778 from: 1 to: 5710
pFN21AB7661 5710 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2356 2356
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2988 3053 1937
Size: 1051 65 4594
Fragments arranged by size:
4594 1051 65
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3773 5157 5425 1632
Size: 2141 1384 268 1917
Fragments arranged by size:
2141 1917 1384 268
AfeI AGC'GCT
Cuts at: 2585 2585
Size: 5710
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 2071 4439 2071
Size: 2368 3342
AleI CACnn'nnGTG
Cuts at: 1166 2682 1166
Size: 1516 4194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2558 2590 2558
Size: 32 5678
AlwNI CAG_nnn'CTG
Cuts at: 2167 2220 2536 3453 5025 2167
Size: 53 316 917 1572 2852
Fragments arranged by size:
2852 1572 917 316 53
ApaLI G'TGCA_C
Cuts at: 2999 3767 4923 2999
Size: 768 1156 3786
Fragments arranged by size:
3786 1156 768
ApoI r'AATT_y
Cuts at: 1493 3019 3245 3397 5506 1493
Size: 1526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2117 2156 3035 1870
Size: 73 77 97 39 879 4545
Fragments arranged by size:
4545 879 97 77 73 39
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 3031 4486 618
Size: 325 217 627 539 705 1455 1842
Fragments arranged by size:
1842 1455 705 627 539 325 217
BanII G_rGCy'C
Cuts at: 1391 2459 3029 1391
Size: 1068 570 4072
Fragments arranged by size:
4072 1068 570
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2047 3602 4812 2047
Size: 1555 1210 2945
Fragments arranged by size:
2945 1555 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 2335 2864
Size: 107 122 71 631 605 568 95 529
Cuts at: 2864 4321 136
Size: 1457 1525
Fragments arranged by size:
1525 1457 631 605 568 529 122 107
95 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 2918 3127 2918
Size: 209 5501
Bme1580I G_kGCm'C
Cuts at: 1790 2285 2331 3003 3771 4927 1790
Size: 495 46 672 768 1156 2573
Fragments arranged by size:
2573 1156 768 672 495 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2133 3441 4399
Size: 416 53 83 399 24 716 1308 958
Cuts at: 4399 442
Size: 1753
Fragments arranged by size:
1753 1308 958 716 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2743 2371
Size: 372 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2152 2381 2473 4370 1239
Size: 913 229 92 1897 2579
Fragments arranged by size:
2579 1897 913 229 92
Bpu10I CC'TnA_GC
Cuts at: 2274 2274
Size: 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 2753 2768 3835 4700 4998 2381
Size: 372 15 1067 865 298 3093
Fragments arranged by size:
3093 1067 865 372 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2120 4373 882
Size: 617 621 2253 2219
Fragments arranged by size:
2253 2219 621 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2958 4143 4541 4815 4962 5373 2958
Size: 1185 398 274 147 411 3295
Fragments arranged by size:
3295 1185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2125 2155 2558 2588 1178
Size: 30 165 30 722 30 403 30 4300
Fragments arranged by size:
4300 722 403 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2282 2363 2582 2714 1186
Size: 1084 12 81 219 132 4182
Fragments arranged by size:
4182 1084 219 132 81 12
BseYI C'CCAG_C
Cuts at: 2042 2220 2305 2459 2976 4913 5409 2042
Size: 178 85 154 517 1937 496 2343
Fragments arranged by size:
2343 1937 517 496 178 154 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2390 2489 2594 1163
Size: 573 654 99 105 4279
Fragments arranged by size:
4279 654 573 105 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2355 2397 2496 3003 3771 3856 4927 2355
Size: 42 99 507 768 85 1071 3138
Fragments arranged by size:
3138 1071 768 507 99 85 42
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
BsmBI CGTCTCn'nnnn_
Cuts at: 2116 2402 2116
Size: 286 5424
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2331 2355 2397 2459 2496 3003
Size: 399 495 46 24 42 62 37 507
Cuts at: 3003 3029 3771 3856 4927 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 507 495 399 85 62
46 42 37 26 24
BspEI T'CCGG_A
Cuts at: 2958 5373 2958
Size: 2415 3295
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2356 3065 844
Size: 1512 709 3489
Fragments arranged by size:
3489 1512 709
BsrBI CCG'CTC
Cuts at: 1394 1823 2656 2925 3597 1394
Size: 429 833 269 672 3507
Fragments arranged by size:
3507 833 672 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 2470 4205 4379 65
Size: 1183 1222 1735 174 1396
Fragments arranged by size:
1735 1396 1222 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2502 4354 4541 1700
Size: 110 692 1852 187 2869
Fragments arranged by size:
2869 1852 692 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 2798 2383
Size: 415 5295
BstYI r'GATC_y
Cuts at: 1972 2440 3040 3407 3792 3809 5261 5347 5359
Size: 468 600 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 600 468 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2208 2265 2208
Size: 57 5653
BtgI C'CryG_G
Cuts at: 513 1065 2004 2376 2445 2514 513
Size: 552 939 372 69 69 3709
Fragments arranged by size:
3709 939 552 372 69 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 2115 2285 3226 4020 4040 2115
Size: 170 941 794 20 3785
Fragments arranged by size:
3785 941 794 170 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2800 3157 4046
Size: 54 1425 50 275 18 970 357 889
Cuts at: 4046 5675 8
Size: 1629 43
Fragments arranged by size:
1629 1425 970 889 357 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3640 1353
Size: 2287 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2161 2567 2921 4297 4671 4817
Size: 1064 138 572 406 354 1376 374 146
Cuts at: 4817 387
Size: 1280
Fragments arranged by size:
1376 1280 1064 572 406 374 354 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2381 2473 3773 4370 5157 5425
Size: 393 520 229 92 1300 597 787 268
Cuts at: 5425 1239
Size: 1524
Fragments arranged by size:
1524 1300 787 597 520 393 268 229
92
EcoO109I rG'GnC_Cy
Cuts at: 2164 2233 2164
Size: 69 5641
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 5710
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2016 2587 2983 4857 1678
Size: 338 571 396 1874 2531
Fragments arranged by size:
2531 1874 571 396 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2125 2157 4351
Size: 32 135 32 485 32 36 32 2194
Cuts at: 4351 4383 4425 4457 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2194 485 135 42 36 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2544 4824 5008 1305
Size: 1239 2280 184 2007
Fragments arranged by size:
2280 2007 1239 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2682 2796 3668 4027 4186 518
Size: 648 1215 301 114 872 359 159 2042
Fragments arranged by size:
2042 1215 872 648 359 301 159 114
MspA1I CmG'CkG
Cuts at: 1606 2102 2220 2309 2639 3126 3803 4951 5196
Size: 496 118 89 330 487 677 1148 245
Cuts at: 5196 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 496 487 330 245 118
89
NcoI C'CATG_G
Cuts at: 513 1065 2004 2376 2445 2514 513
Size: 552 939 372 69 69 3709
Fragments arranged by size:
3709 939 552 372 69 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2200 3068 4613 1132
Size: 97 971 868 1545 2229
Fragments arranged by size:
2229 1545 971 868 97
PasI CC'CwG_GG
Cuts at: 1348 2237 2593 1348
Size: 889 356 4465
Fragments arranged by size:
4465 889 356
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2451 2536 1108
Size: 1343 85 4282
Fragments arranged by size:
4282 1343 85
PfoI T'CCnGG_A
Cuts at: 2529 2529
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2558 2590 3779 3811 5292 5324 2558
Size: 32 1189 32 1481 32 2944
Fragments arranged by size:
2944 1481 1189 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2133 1613
Size: 520 5190
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 3062 830
Size: 2232 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2220 2220
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 3058 4193 4874 5065 826
Size: 219 2013 1135 681 191 1471
Fragments arranged by size:
2013 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2240 2240
Size: 5710
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 2022 3037 1872
Size: 150 1015 4545
Fragments arranged by size:
4545 1015 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 2396 2768 2783 3814 4715
Size: 197 926 174 279 372 15 1031 901
Cuts at: 4715 4977 820
Size: 262 1553
Fragments arranged by size:
1553 1031 926 901 372 279 262 197
174 15
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 2525 1517
Size: 1008 4702
StyI C'CwwG_G
Cuts at: 513 1065 2004 2229 2343 2376 2445 2514 3149
Size: 552 939 225 114 33 69 69 635
Cuts at: 3149 513
Size: 3074
Fragments arranged by size:
3074 939 635 552 225 114 69 69
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2585 2726 3737 3754 3907 4092 4537
Size: 1255 1005 141 1011 17 153 185 445
Cuts at: 4537 325
Size: 1498
Fragments arranged by size:
1498 1255 1011 1005 445 185 153 141
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2425 2457 5670 5702 2425
Size: 32 3213 32 2433
Fragments arranged by size:
3213 2433 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 2117 1943
Size: 174 5536
XmaI C'CCGG_G
Cuts at: 1870 2020 3035 1870
Size: 150 1015 4545
Fragments arranged by size:
4545 1015 150
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI XcmI