Restriction map for
pFN21AB7643
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaHI BsaI
BsaWI BseAI BseRI BsiWI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DdeI DpnII DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI HphI KasI
KpnI KspI MboI MflI MluI
MroI MseI MspA1I MvnI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI Sau3AI SbfI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tth111I XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 337 GAATTC [GA]^AATT[CT]
AflIII 218 ACGTGT A^C[GA][CT]GT
Alw44I 715 GTGCAC G^TGCAC
ApaLI 715 GTGCAC G^TGCAC
ApoI 337 GAATTC [GA]^AATT[CT]
Asp700I 507 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 715 GTGCAC G[AT]GC[AT]^C
BalI 816 TGGCCA TGG^CCA
BanII 416 GAGCCC G[GA]GC[CT]^C
BfaI 832 CTAG C^TAG
BglI 259 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 55 CTCCAG CTGGAG(16/14)
BsaAI 217 TACGTG [CT]AC^GT[GA]
BsaBI 3 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 411 CGACCG CG[GA][CT]^CG
BsiHKAI 715 GTGCAC G[AT]GC[AT]^C
BsmBI 736 GAGACG CGTCTC(1/5)
BsmI 446 GCATTC GAATGC(1/-1)
BsrBI 37 GAGCGG CCGCTC(-3/-3)
BsrDI 586 CATTGC GCAATG(2/0)
BsrI 885 CCAGT ACTGG(1/-1)
BstF5I 794 GGATG GGATG(2/0)
EaeI 816 TGGCCA [CT]^GGCC[GA]
EcoRI 337 GAATTC G^AATTC
EcoT14I 864 CCAAGG C^C[AT][AT]GG
FokI 794 GGATG GGATG(9/13)
HincII 478 GTCAAC GT[CT]^[GA]AC
HindII 478 GTCAAC GT[CT]^[GA]AC
MaeI 832 CTAG C^TAG
MaeII 218 ACGT A^CGT
MamI 3 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 751 CAATTG C^AATTG
MluNI 816 TGGCCA TGG^CCA
MscI 816 TGGCCA TGG^CCA
MunI 751 CAATTG C^AATTG
PaeR7I 209 CTCGAG C^TCGAG
PflMI 885 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 706 GGGACCC [GA]G^G[AT]CC[CT]
SapI 675 GCTCTTC GCTCTTC(1/4)
ScaI 509 AGTACT AGT^ACT
SexAI 151 ACCTGGT A^CC[AT]GGT
SfiI 258 GGCCTTCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 304 CCCGGG CCC^GGG
SmlI 209 CTCGAG C^T[CT][GA]AG
StyI 864 CCAAGG C^C[AT][AT]GG
Tsp45I 119 GTGAC ^GT[GC]AC
Van91I 885 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 209 CTCGAG C^TCGAG
XmaCI 304 CCCGGG C^CCGGG
XmaI 304 CCCGGG C^CCGGG
XmnI 507 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 537 AGCT AG^CT
616 AGCT
AvaI 209 CTCGAG C^[CT]CG[GA]G
304 CCCGGG
BsgI 726 CTGCAC GTGCAG(16/14)
612 GTGCAG
BsmAI 212 GAGAC GTCTC(1/5)
736 GAGAC
BsoBI 209 CTCGAG C^[CT]CG[GA]G
304 CCCGGG
Bsp1407I 377 TGTACA T^GTACA
530 TGTACA
BspMI 251 ACCTGC ACCTGC(4/8)
369 GCAGGT
BsrGI 377 TGTACA T^GTACA
530 TGTACA
DraII 668 AGGGCCT [GA]G^G[ACGT]CC[CT]
706 GGGACCC
EarI 676 CTCTTC CTCTTC(1/4)
763 GAAGAG
EcoO109I 668 AGGGCCT [GA]G^G[ACGT]CC[CT]
706 GGGACCC
HapII 285 CCGG C^CGG
305 CCGG
HpaII 285 CCGG C^CGG
305 CCGG
Ksp632I 676 CTCTTC CTCTTC(1/4)
763 GAAGAG
MaeIII 119 GTGAC ^GT[ACGT]AC
148 GTTAC
MspI 285 CCGG C^CGG
305 CCGG
PstI 396 CTGCAG CTGCA^G
426 CTGCAG
SfcI 396 CTGCAG C^T[GA][CT]AG
426 CTGCAG
SspBI 377 TGTACA T^GTACA
530 TGTACA
TaqI 210 TCGA T^CGA
344 TCGA
TthHB8I 210 TCGA T^CGA
344 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AfaI AvaII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I Cfr13I
EcoRII Fnu4HI HaeIII HinfI ItaI
MboII MnlI MslI MvaI MwoI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI TfiI TseI Tsp509I
TspRI