(Circular) (Six-base) MAPSORT of: pFN21AB7643.seq Check: 9907 from: 1 to: 5602 pFN21AB7643 5602 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2365 2365 Size: 5602 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2945 1937 Size: 1008 4594 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3665 5049 5317 1632 Size: 2033 1384 268 1917 Fragments arranged by size: 2033 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 2223 4501 5478 1203 Size: 1020 2278 977 1327 Fragments arranged by size: 2278 1327 1020 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 2433 2642 2699 1166 Size: 1267 209 57 4069 Fragments arranged by size: 4069 1267 209 57 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AlwNI CAG_nnn'CTG Cuts at: 3345 4917 3345 Size: 1572 4030 ApaLI G'TGCA_C Cuts at: 2720 3659 4815 2720 Size: 939 1156 3507 Fragments arranged by size: 3507 1156 939 ApoI r'AATT_y Cuts at: 1493 2342 2911 3137 3289 5398 1493 Size: 849 569 226 152 2109 1697 Fragments arranged by size: 2109 1697 849 569 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2309 2927 1870 Size: 73 271 95 618 4545 Fragments arranged by size: 4545 618 271 95 73 BamHI G'GATC_C Cuts at: 2932 3299 2932 Size: 367 5235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2923 4378 618 Size: 325 217 627 1136 1455 1842 Fragments arranged by size: 1842 1455 1136 627 325 217 BanII G_rGCy'C Cuts at: 1391 2425 2921 1391 Size: 1034 496 4072 Fragments arranged by size: 4072 1034 496 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3793 3827 1903 Size: 34 1856 34 3678 Fragments arranged by size: 3678 1856 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3494 4704 3494 Size: 1210 4392 BclI T'GATC_A Cuts at: 1548 1548 Size: 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2270 4213 136 Size: 107 122 71 631 605 598 1943 1525 Fragments arranged by size: 1943 1525 631 605 598 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 3019 3019 Size: 5602 Bme1580I G_kGCm'C Cuts at: 1790 2322 2393 2724 3663 4819 1790 Size: 532 71 331 939 1156 2573 Fragments arranged by size: 2573 1156 939 532 331 71 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3333 4291 442 Size: 416 53 83 399 24 1916 958 1753 Fragments arranged by size: 1916 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 4262 1239 Size: 806 2217 2579 Fragments arranged by size: 2579 2217 806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4265 882 Size: 617 2766 2219 Fragments arranged by size: 2766 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2224 3393 493 Size: 711 1020 1169 2702 Fragments arranged by size: 2702 1169 1020 711 BsaBI GATnn'nnATC Cuts at: 2012 5596 2012 Size: 3584 2018 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3409 3791 4426 275 Size: 53 83 186 936 1876 382 635 1451 Fragments arranged by size: 1876 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4035 4433 4707 4854 5265 4035 Size: 398 274 147 411 4372 Fragments arranged by size: 4372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5377 Fragments arranged by size: 5377 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5602 BseYI C'CCAG_C Cuts at: 2841 4805 5301 2841 Size: 1964 496 3142 Fragments arranged by size: 3142 1964 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2638 2716 1163 Size: 573 902 78 4049 Fragments arranged by size: 4049 902 573 78 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2419 3052 3813 4841 1540 Size: 275 186 418 633 761 1028 2301 Fragments arranged by size: 2301 1028 761 633 418 275 186 BsiHKAI G_wGCw'C Cuts at: 2724 3663 3748 4819 2724 Size: 939 85 1071 3507 Fragments arranged by size: 3507 1071 939 85 BsmI GAATG_Cn' Cuts at: 2451 3113 3206 2451 Size: 662 93 4847 Fragments arranged by size: 4847 662 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2735 2735 Size: 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 2393 2425 2724 2921 3663 3748 Size: 399 532 71 32 299 197 742 85 Cuts at: 3748 4819 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 532 399 299 197 85 71 32 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 3491 5221 3491 Size: 1730 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2265 2365 2957 844 Size: 1421 100 592 3489 Fragments arranged by size: 3489 1421 592 100 BsrBI CCG'CTC Cuts at: 1394 1823 2044 3489 1394 Size: 429 221 1445 3507 Fragments arranged by size: 3507 1445 429 221 BsrDI GCAATG_nn' Cuts at: 65 1248 2590 4097 4271 65 Size: 1183 1342 1507 174 1396 Fragments arranged by size: 1507 1396 1342 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 2382 2535 96 Size: 2286 153 3163 Fragments arranged by size: 3163 2286 153 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2932 3299 3684 3701 5153 5239 5251 5597 Size: 960 367 385 17 1452 86 12 346 Cuts at: 5597 1972 Size: 1977 Fragments arranged by size: 1977 1452 960 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5050 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2122 487 Size: 683 56 896 3967 Fragments arranged by size: 3967 896 683 56 BtsI GCAGTG_nn' Cuts at: 2284 2322 2428 2440 3118 3912 3932 2284 Size: 38 106 12 678 794 20 3954 Fragments arranged by size: 3954 794 678 106 38 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2821 3049 3938 Size: 54 1425 50 275 18 991 228 889 Cuts at: 3938 5567 8 Size: 1629 43 Fragments arranged by size: 1629 1425 991 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2687 2763 3532 1353 Size: 1334 76 769 3423 Fragments arranged by size: 3423 1334 769 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4189 4563 4709 387 Size: 1064 138 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 3665 4262 5049 5317 1239 Size: 393 413 1620 597 787 268 1524 Fragments arranged by size: 1620 1524 787 597 413 393 268 EcoO109I rG'GnC_Cy Cuts at: 2674 2712 2674 Size: 38 5564 EcoRI G'AATT_C Cuts at: 2342 2911 3289 2342 Size: 569 378 4655 Fragments arranged by size: 4655 569 378 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 4749 1678 Size: 3071 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2197 2229 4243 4275 4317 Size: 32 135 32 625 32 2014 32 42 Cuts at: 4317 4349 1373 Size: 32 2626 Fragments arranged by size: 2626 2014 625 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2485 2946 3192 669 Size: 988 281 547 461 246 3079 Fragments arranged by size: 3079 988 547 461 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 2756 3201 2756 Size: 445 5157 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4716 4900 1305 Size: 3411 184 2007 Fragments arranged by size: 3411 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2823 10 Size: 54 1425 343 991 2789 Fragments arranged by size: 2789 1425 991 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2433 2570 2642 2699 3560 3919 4078 Size: 648 1267 137 72 57 861 359 159 Cuts at: 4078 518 Size: 2042 Fragments arranged by size: 2042 1267 861 648 359 159 137 72 57 MspA1I CmG'CkG Cuts at: 1606 3018 3695 4843 5088 1606 Size: 1412 677 1148 245 2120 Fragments arranged by size: 2120 1412 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5050 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2960 4505 1132 Size: 97 1731 1545 2229 Fragments arranged by size: 2229 1731 1545 97 PasI CC'CwG_GG Cuts at: 1348 2478 1348 Size: 1130 4472 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 2896 1108 Size: 1788 3814 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 5184 5216 3671 Size: 32 1481 32 4057 Fragments arranged by size: 4057 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2712 2712 Size: 5602 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PstI C_TGCA'G Cuts at: 830 2405 2435 2954 830 Size: 1575 30 519 3478 Fragments arranged by size: 3478 1575 519 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SanDI GG'GwC_CC Cuts at: 2712 2712 Size: 5602 SapI GCTCTTCn'nnn_ Cuts at: 2687 2687 Size: 5602 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 2516 3850 5562 2516 Size: 1334 1712 2556 Fragments arranged by size: 2556 1712 1334 SexAI A'CCwGG_T Cuts at: 2156 2156 Size: 5602 SfcI C'TryA_G Cuts at: 826 1045 2401 2431 2950 4085 4766 4957 826 Size: 219 1356 30 519 1135 681 191 1471 Fragments arranged by size: 1471 1356 1135 681 519 219 191 30 SfiI GGCCn_nnn'nGGCC Cuts at: 2270 2270 Size: 5602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2311 2929 1872 Size: 439 618 4545 Fragments arranged by size: 4545 618 439 SmlI C'TyrA_G Cuts at: 820 1017 1943 2214 3706 4607 4869 820 Size: 197 926 271 1492 901 262 1553 Fragments arranged by size: 1553 1492 926 901 271 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2869 3041 513 Size: 552 1804 172 3074 Fragments arranged by size: 3074 1804 552 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2036 2433 2650 3629 3646 3799 3984 Size: 1255 456 397 217 979 17 153 185 Cuts at: 3984 4429 325 Size: 445 1498 Fragments arranged by size: 1498 1255 979 456 445 397 217 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2382 2514 2535 Size: 274 80 33 51 1443 405 132 21 Cuts at: 2535 3848 5560 96 Size: 1313 1712 138 Fragments arranged by size: 1712 1443 1313 405 274 138 132 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 2214 1943 Size: 271 5331 XmaI C'CCGG_G Cuts at: 1870 2309 2927 1870 Size: 439 618 4545 Fragments arranged by size: 4545 618 439 XmnI GAAnn'nnTTC Cuts at: 2516 3731 2516 Size: 1215 4387 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SfoI SrfI SwaI XcmI